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1. 发明申请

WO2012045088A2 COMBINATORIAL DESIGN OF HIGHLY EFFICIENT HETEROLOGOUS PATHWAYS 审中-公开
标题翻译：高效率异质途径的组合设计
公开(公告)号：WO2012045088A2
公开(公告)日：2012-04-05
申请号：PCT/US2011/054650
申请日：2011-10-03
申请人： THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS , ZHAO, Huimin , KIM, Byoungjin , DU, Jing , YUAN, Yongbo , ERIKSEN, Dawn , SI, Tong
发明人： ZHAO, Huimin , KIM, Byoungjin , DU, Jing , YUAN, Yongbo , ERIKSEN, Dawn , SI, Tong
IPC分类号： C12N15/52
CPC分类号： C12P7/06 , C07K14/39 , C12N9/0006 , C12N9/1205 , C12N9/2445 , C12N15/1093 , C12N15/52 , C12N15/81 , C12P7/10 , C12Y101/01009 , C12Y101/0101 , C12Y101/01307 , C12Y207/01017 , C12Y302/01021 , Y02E50/16 , Y02E50/17
摘要： The present disclosure relates to the production of highly efficient heterologous pathways in host cells by identifying favorable enzyme and/or promoter combinations. In particular the present disclosure provides methods for assembly and selection of multi-step xylose and arabinose/xylose utilization pathways from a library of fungal enzymes. The present disclosure further provides compositions containing favorable enzyme combinations, as well as recombinant yeast expressing such combinations, and methods of use for bioconversion of pentose sugars. Also provided are compositions and methods involving favorable expression patterns identified by utilization of combinations of promoters of varying strengths. Provided herein are methods for assembly and selection of multi-step xylose, arabinose/xylose, and cellobiose utilization pathways from a library of promoters of varying strengths. The present disclosure further provides compositions containing heterologous enzyme-coding polynucleotides under the control of favorable promoters, as well as recombinant yeast expressing such enzymes, and methods of their use for bioconversion of pentose and/or hexose sugars.
摘要翻译：本公开涉及通过鉴定有利的酶和/或启动子组合而在宿主细胞中产生高效异源途径。特别地，本公开提供了用于从真菌酶库组装和选择多步骤木糖和阿拉伯糖/木糖利用途径的方法。本公开内容进一步提供了含有有利的酶组合的组合物，以及表达这种组合的重组酵母，以及用于生物转化戊糖的方法。还提供了涉及利用不同强度的启动子组合鉴定的有利表达模式的组合物和方法。本文提供了用于从不同强度的启动子文库组装和选择多步骤木糖，阿拉伯糖/木糖和纤维二糖利用途径的方法。本发明进一步提供了在有利启动子控制下含有异源酶编码多核苷酸的组合物，以及表达这些酶的重组酵母，以及它们用于生物转化戊糖和/或己糖的方法。

2. 发明申请

WO2012045088A9 COMBINATORIAL DESIGN OF HIGHLY EFFICIENT HETEROLOGOUS PATHWAYS 审中-公开
标题翻译：高效率异常路径的组合设计
公开(公告)号：WO2012045088A9
公开(公告)日：2012-07-26
申请号：PCT/US2011054650
申请日：2011-10-03
申请人： UNIV ILLINOIS , ZHAO HUIMIN , KIM BYOUNGJIN , DU JING , YUAN YONGBO , ERIKSEN DAWN , SI TONG
发明人： ZHAO HUIMIN , KIM BYOUNGJIN , DU JING , YUAN YONGBO , ERIKSEN DAWN , SI TONG
IPC分类号： C12N15/52
CPC分类号： C12P7/06 , C07K14/39 , C12N9/0006 , C12N9/1205 , C12N9/2445 , C12N15/1093 , C12N15/52 , C12N15/81 , C12P7/10 , C12Y101/01009 , C12Y101/0101 , C12Y101/01307 , C12Y207/01017 , C12Y302/01021 , Y02E50/16 , Y02E50/17
摘要： The present disclosure relates to the production of highly efficient heterologous pathways in host cells by identifying favorable enzyme and/or promoter combinations. In particular the present disclosure provides methods for assembly and selection of multi-step xylose and arabinose/xylose utilization pathways from a library of fungal enzymes. The present disclosure further provides compositions containing favorable enzyme combinations, as well as recombinant yeast expressing such combinations, and methods of use for bioconversion of pentose sugars. Also provided are compositions and methods involving favorable expression patterns identified by utilization of combinations of promoters of varying strengths. Provided herein are methods for assembly and selection of multi-step xylose, arabinose/xylose, and cellobiose utilization pathways from a library of promoters of varying strengths. The present disclosure further provides compositions containing heterologous enzyme-coding polynucleotides under the control of favorable promoters, as well as recombinant yeast expressing such enzymes, and methods of their use for bioconversion of pentose and/or hexose sugars.
摘要翻译：本公开涉及通过鉴定有利的酶和/或启动子组合在宿主细胞中产生高效的异源途径。特别地，本公开提供了从真菌酶文库中组装和选择多步木糖和阿拉伯糖/木糖利用途径的方法。本公开进一步提供含有有利的酶组合的组合物，以及表达这种组合的重组酵母以及用于戊糖的生物转化的方法。还提供了包括通过利用不同强度的启动子的组合鉴定的有利的表达模式的组合物和方法。本文提供了用于从不同强度的启动子文库中组装和选择多步木糖，阿拉伯糖/木糖和纤维二糖利用途径的方法。本公开进一步提供了含有有利启动子控制的异源酶编码多核苷酸的组合物，以及表达这些酶的重组酵母，以及它们用于戊糖和/或己糖的生物转化的方法。

3. 发明申请

WO2012045088A3 COMBINATORIAL DESIGN OF HIGHLY EFFICIENT HETEROLOGOUS PATHWAYS 审中-公开
标题翻译：高效率异质途径的组合设计
公开(公告)号：WO2012045088A3
公开(公告)日：2012-09-13
申请号：PCT/US2011054650
申请日：2011-10-03
申请人： UNIV ILLINOIS , ZHAO HUIMIN , KIM BYOUNGJIN , DU JING , YUAN YONGBO , ERIKSEN DAWN , SI TONG
发明人： ZHAO HUIMIN , KIM BYOUNGJIN , DU JING , YUAN YONGBO , ERIKSEN DAWN , SI TONG
IPC分类号： C12N15/52 , C12N9/02 , C12N9/04 , C12N9/12 , C12N15/10 , C12P7/06 , C12P7/08
CPC分类号： C12P7/06 , C07K14/39 , C12N9/0006 , C12N9/1205 , C12N9/2445 , C12N15/1093 , C12N15/52 , C12N15/81 , C12P7/10 , C12Y101/01009 , C12Y101/0101 , C12Y101/01307 , C12Y207/01017 , C12Y302/01021 , Y02E50/16 , Y02E50/17
摘要： The present disclosure relates to the production of highly efficient heterologous pathways in host cells by identifying favorable enzyme and/or promoter combinations. In particular the present disclosure provides methods for assembly and selection of multi-step xylose and arabinose/xylose utilization pathways from a library of fungal enzymes. The present disclosure further provides compositions containing favorable enzyme combinations, as well as recombinant yeast expressing such combinations, and methods of use for bioconversion of pentose sugars. Also provided are compositions and methods involving favorable expression patterns identified by utilization of combinations of promoters of varying strengths. Provided herein are methods for assembly and selection of multi-step xylose, arabinose/xylose, and cellobiose utilization pathways from a library of promoters of varying strengths. The present disclosure further provides compositions containing heterologous enzyme-coding polynucleotides under the control of favorable promoters, as well as recombinant yeast expressing such enzymes, and methods of their use for bioconversion of pentose and/or hexose sugars.
摘要翻译：本公开涉及通过鉴定有利的酶和/或启动子组合来在宿主细胞中产生高效异源途径。特别地，本公开提供了用于从真菌酶库组装和选择多步骤木糖和阿拉伯糖/木糖利用途径的方法。本公开内容进一步提供了含有有利的酶组合的组合物，以及表达这种组合的重组酵母，以及用于生物转化戊糖的方法。还提供了涉及利用不同强度的启动子组合鉴定的有利表达模式的组合物和方法。本文提供了用于从不同强度的启动子文库组装和选择多步骤木糖，阿拉伯糖/木糖和纤维二糖利用途径的方法。本发明进一步提供了在有利启动子控制下含有异源酶编码多核苷酸的组合物，以及表达这些酶的重组酵母，以及它们用于生物转化戊糖和/或己糖的方法。

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