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1. 发明申请

WO2007128496A3 A GROUP OF ESTERASES FOR THE ENANTIOSELECTIVE PRODUCTION OF FINE AND SPECIALITY CHEMICALS 审中-公开
标题翻译：一组用于精细和特殊化学品生产的产品
公开(公告)号：WO2007128496A3
公开(公告)日：2008-02-21
申请号：PCT/EP2007003919
申请日：2007-05-03
申请人： B R A I N AG , LIEBETON KLAUS , ECK JUERGEN , BORNSCHEUER UWE , BOETTCHER DOMINIQUE , LANGER PETER , BELLUR ESEN
发明人： LIEBETON KLAUS , ECK JUERGEN , BORNSCHEUER UWE , BOETTCHER DOMINIQUE , LANGER PETER , BELLUR ESEN
IPC分类号： C12N9/18 , C07K16/40 , C12N5/10 , C12N15/55
CPC分类号： C12N9/18
摘要： The present invention relates to a polynucleotide encoding an enzyme having carboxylesterase [E. C. 3.1.1.1] activity, wherein the coding sequence is selected from the group consisting of (a) a polynucleotide encoding an amino acid sequence as depicted in any one of SEQ ID NOs: 2, 4, 6, 8, 10 and 12; (b) a polynucleotide having or comprising a nucleotide sequence encoding an enzyme, wherein the nucleic acid sequence is as shown in any one of SEQ ID NOs: 1, 3, 5, 7, 9 and 11; (c) a polynucleotide having or comprising a nucleotide sequence encoding a fragment or derivative of an enzyme encoded by a polynucleotide of (a) or (b), wherein in said derivative one or more amino acid residues are conservatively substituted compared to the amino acid sequence of (a).
摘要翻译：本发明涉及编码具有羧酸酯酶[E. 3.1.1.1]活性，其中编码序列选自（a）编码SEQ ID NO：2,4,6,8,10和12中任一个所示的氨基酸序列的多核苷酸 ; （b）具有或包含编码酶的核苷酸序列的多核苷酸，其中所述核酸序列如SEQ ID NO：1,3,5,7,9和11中任一个所示; （c）具有或包含编码由（a）或（b）的多核苷酸编码的酶的片段或衍生物的核苷酸序列的多核苷酸，其中在所述衍生物中，与氨基酸相比，一个或多个氨基酸残基被保守取代（a）的顺序。

2. 发明申请

WO9829540A3 RECOMBINANT FUSION PROTEINS BASED ON RIBOSOME-INACTIVATING PROTEINS OF EUROPEAN MISTLETOE VISCUM ALBUM 审中-公开
标题翻译：基于欧洲麻醉药物抗坏血酸蛋白酶抑制蛋白的重组融合蛋白
公开(公告)号：WO9829540A3
公开(公告)日：1998-11-12
申请号：PCT/EP9800009
申请日：1998-01-02
申请人： B R A I N BIOTECHNOLOGY RESEAR , ECK JUERGEN , SCHMIDT ARNO , ZINKE HOLGER
发明人： ECK JUERGEN , SCHMIDT ARNO , ZINKE HOLGER
IPC分类号： A61K38/00 , A61K38/16 , A61K47/48 , C07K14/42 , C07K19/00 , C12N15/29 , C12N15/62 , C12P21/02 , C12Q1/68 , C12N15/00
CPC分类号： C07K14/42 , A61K38/00 , C07K2319/02 , C07K2319/21 , C07K2319/50 , C07K2319/55 , C07K2319/75 , C12N15/62
摘要： Nucleic acid molecules code for fusion proteins which contain at least one effector module, a processing module and a targeting module. The disclosed nucleic acid molecules preferably further contain a modulator module and/or an affinity module. Also disclosed are vectors containing these nucleic acid molecules, hosts transformed by the disclosed vectors, fusion proteins coded by the disclosed nucleic acids or produced by the disclosed hosts, and medicaments which contain the disclosed polypeptides or vectors. These medicaments are particularly significant for the therapy of diseases associated with a pathological reproduction and/or increased activity of cell populations. A temporary, abrupt and strong proliferation, infiltration and immune activity of cells of the immune system is found in auto-immune diseases and allergies, the specificity of these immune cells being due to their reaction to a particular antigen or allergen. These medicaments may also be advantageously used for treating tumours. The disclosed polypeptides and vectors can be used to develop medicaments and to test toxin activity-modulating factors. The invention thus also concerns corresponding processes, uses and kits. The modules, with the exception of the affinity and targeting modules, are preferably coded by nucleic acids extracted or derived from the mistletoe lectin proprotein coding sequence.

3. 发明申请

WO2007115797A2 A GROUP OF NOVEL ENANTIOSELECTIVE MICROBIAL NITRILE HYDRATASES WITH BROAD SUBSTRATE SPECIFICITY 审中-公开
标题翻译：一种具有广泛基质特征的新型微囊藻酸盐水溶液
公开(公告)号：WO2007115797A2
公开(公告)日：2007-10-18
申请号：PCT/EP2007003114
申请日：2007-04-05
申请人： B R A I N AG , ECK JUERGEN , LIEBETON KLAUS , FISCHER LUTZ , LUTZ-WAHL SABINE , HENSEL MARTINA , EWERT CHRISTIAN , WAELZ MARCUS
发明人： ECK JUERGEN , LIEBETON KLAUS , FISCHER LUTZ , LUTZ-WAHL SABINE , HENSEL MARTINA , EWERT CHRISTIAN , WAELZ MARCUS
IPC分类号： C12N9/14 , C07K14/195 , C12N1/20 , C12N9/78 , C12P13/02 , C12P41/00
CPC分类号： C12N9/88 , C12P13/02 , C12P41/006
摘要： The present invention provides a polynucleotide or a pair of polynucleotides encoding an enzyme having nitrile hydratase (NHase) [E. C. 4.2.1.84] activity. Furthermore, a vector and a host comprising the disclosed polynucleotide or pair of polynucleotides and methods for the production of the same are provided. Moreover, the invention relates to a pair of polypeptides or a fusion protein having NHase activity, an antibody specifically binding to the pair of polypeptides or fusion protein, a primer or probe, which specifically hybridizes under stringent conditions to the disclosed polynucleotide or either one of the pair of polynucleotides, a composition comprising the polynucleotide or pair of polynucleotides, the pair of polypeptides or fusion protein, the antibody and/or one or more primers or probes of the invention and a method for the production of amides comprising the enantioselective conversion of nitriles.
摘要翻译：本发明提供了编码具有腈水合酶（NHase）的酶的多核苷酸或一对多核苷酸。 C. 4.2.1.84]活动。此外，提供了包含所公开的多核苷酸或一对多核苷酸的载体和宿主及其生产方法。此外，本发明涉及一对多肽或具有NHase活性的融合蛋白，特异性结合所述一对多肽或融合蛋白的抗体，在严格条件下与所公开的多核苷酸特异性杂交的引物或探针，或者一对多核苷酸，包含多核苷酸或一对多核苷酸的组合物，一对多肽或融合蛋白，本发明的抗体和/或一种或多种引物或探针以及生产酰胺的方法，其包括对映选择性转化腈。

4. 发明申请

WO2005103239A3 NOVEL ALCOHOL DEHYDROGENASES 审中-公开
标题翻译：新型酒精脱水剂
公开(公告)号：WO2005103239A3
公开(公告)日：2006-03-09
申请号：PCT/EP2005002557
申请日：2005-03-10
申请人： DEGUSSA , SCHULZE RENATE , LORENZ PATRICK , ECK JUERGEN , MAY OLIVER , GROEGER HARALD , TRAUTHWEIN HARALD
发明人： SCHULZE RENATE , LORENZ PATRICK , ECK JUERGEN , MAY OLIVER , GROEGER HARALD , TRAUTHWEIN HARALD
IPC分类号： C12N9/04 , C12N15/31
CPC分类号： C12P7/02 , C12N9/0006 , C12Y101/01002
摘要： The invention relates to novel polypeptides which have the biological activity of an NAD- or NADP-dependent alcohol dehydrogenase. The invention furthermore relates to nucleic acids encoding said polypeptides, to non-human hosts or host cells and to reaction systems which may be used for preparing desired products. The polypeptides of the invention are preferably used in the preparation, starting from aldehydes or ketones, of primary and enantiomerically pure secondary alcohol's which may serve as intermediates for medicaments. Alternatively, the polypeptides of the invention may also be employed in the reverse reaction, i.e. the oxidation of alcohol's with the formation of aldehydes or ketones.
摘要翻译：本发明涉及具有NAD-或NADP依赖性醇脱氢酶的生物活性的新型多肽。本发明还涉及编码所述多肽，非人宿主或宿主细胞的核酸和可用于制备所需产物的反应体系。本发明的多肽优选用于从醛或酮开始制备可以作为药物中间体的初级和对映异构纯的仲醇。或者，本发明的多肽也可以用于反向反应，即醇与醛或酮的形成的氧化。

5. 发明申请

WO2004018673A3 ISOLATION AND CLONING OF DNA FROM UNCULTIVATED ORGANISMS 审中-公开
标题翻译：分离和克隆来自不同组织的DNA
公开(公告)号：WO2004018673A3
公开(公告)日：2004-08-12
申请号：PCT/EP0309223
申请日：2003-08-20
申请人： B R A I N BIOTECHNOLOGY RES AN , QUAISER ACHIM , OCHSENREITER TORSTEN , TREUSCH ALEXANDER H , KLETZIN ARNULF , SCHLEPER CHRISTA , LORENZ PATRICK , ECK JUERGEN
发明人： QUAISER ACHIM , OCHSENREITER TORSTEN , TREUSCH ALEXANDER H , KLETZIN ARNULF , SCHLEPER CHRISTA , LORENZ PATRICK , ECK JUERGEN
IPC分类号： C12N15/10
CPC分类号： C12N15/1003
摘要： The present invention relates to a device for the isolation and/or purification of nucleic acid molecules suitable to bind and/or inactivate inhibitors of the activity of reagents or enzymes used for DNA manipulation and to separate a plurality of nucleic acid molecules with respect to their size. Moreover, the invention relates to a method for the isolation of a nucleic acid molecule comprising applying a sample to the device of the invention wherein said nucleic acid molecule preferably represents a fraction of the metagenome of a given habitat. Furthermore, the invention relates to a method for the generation of at least one gene library comprising nucleic acid molecules isolated by the method of the invention and to a nucleic acid molecule isolated by the method of the invention.
摘要翻译：本发明涉及用于分离和/或纯化适于结合和/或失活用于DNA操作的试剂或酶的活性抑制剂的核酸分子的装置，并且相对于它们分离多个核酸分子尺寸。此外，本发明涉及用于分离核酸分子的方法，包括将样品施加到本发明的装置中，其中所述核酸分子优选代表给定栖息地的元素的一部分。此外，本发明涉及产生至少一种包含通过本发明方法分离的核酸分子的基因文库以及通过本发明的方法分离的核酸分子的方法。

6. 发明申请

WO2011078667A3 METHOD OF FINDING A BIOCATALYST HAVING AMMONIA LYASE ACTIVITY 审中-公开
标题翻译：寻找具有氨活性的生物催化剂的方法
公开(公告)号：WO2011078667A3
公开(公告)日：2011-08-18
申请号：PCT/NL2010050876
申请日：2010-12-22
申请人： DSM IP ASSETS BV , RAEMAKERS-FRANKEN PETRONELLA CATHARINA , MEURER GUIDO , ECK JUERGEN WOLFGANG , LIEBETON KLAUS JUERGEN , TREFZER AXEL CHRISTOPH
发明人： RAEMAKERS-FRANKEN PETRONELLA CATHARINA , MEURER GUIDO , ECK JUERGEN WOLFGANG , LIEBETON KLAUS JUERGEN , TREFZER AXEL CHRISTOPH
IPC分类号： C12Q1/02 , C12Q1/527
CPC分类号： C12Q1/527 , C07C229/08 , C07C229/26 , C07C233/47 , C12N9/88 , C12P13/005
摘要： A method of finding a biocatalyst having ammonia lyase activity, in particular a lysine ammonia lyase, comprising - providing a library comprising a plurality of cells in one or more cell cultures, which cells are candidates for having an ammonia lyase activity, which one or more cultures comprise a culture medium containing at least one nitrogen source selected from the group of amines, including amino acids, and structural analogues thereof, as sole nitrogen source or sources for the cells; - selecting at least one candidate which grows in said culture medium; and - screening the selected candidate or candidates which grow in said culture for the ammonia lyase activity.
摘要翻译：一种发现具有氨裂解酶活性的生物催化剂，特别是赖氨酸氨裂合酶的方法，包括 - 提供包含一种或多种细胞培养物中的多种细胞的文库，所述细胞是具有氨裂解酶活性的候选物，所述文库中的一种或多种培养物包含培养基，该培养基含有至少一种选自胺（包括氨基酸）及其结构类似物的氮源作为唯一氮源或细胞来源; - 选择在所述培养基中生长的至少一种候选物; 和 - 筛选在所述培养物中生长的选择的一种或多种候选氨裂解酶活性的候选物。

7. 发明申请

WO2010099955A8 PROTEASE FOR WOUND CONDITIONING AND SKIN CARE 审中-公开
标题翻译：保护皮肤护理和皮肤护理
公开(公告)号：WO2010099955A8
公开(公告)日：2010-12-16
申请号：PCT/EP2010001328
申请日：2010-03-03
申请人： BRAIN BIOTECHNOLOGY RES & INFORMATION NETWORK AG , NIEHAUS FRANK , ECK JUERGEN , SCHULZE RENATE
发明人： NIEHAUS FRANK , ECK JUERGEN , SCHULZE RENATE
IPC分类号： C12N9/64 , A61K38/43 , C07K16/40 , C12N5/10 , C12N15/62
CPC分类号： C12N9/6408
摘要： We have identified by molecular cloning a protease which originates from the larvae of Lucilia sericata and which was termed debrilase due to its activities useful for debridement of wounds. Described is a nucleic acid molecule encoding a serine protease having the ability to cleave fibrin and casein which is (a) a nucleic acid molecule encoding the serine protease comprising or consisting of the amino acid sequence of SEQ ID NO: 4 as well as to nucleic acid molecules encoding precursors or fragments of said serine protease; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 3; (c) a nucleic acid molecule encoding a serine protease the amino acid sequence of which is at least 80 % identical to the amino acid sequence of (a), preferably at least 85 % identical, more preferably at least 90 % identical, and most preferred 95% identical; (d) a nucleic acid molecule comprising or consisting of a nucleotide sequence which is at least 80 % identical to the nucleotide sequence of (b), preferably at least 85 % identical, more preferably at least 90 % identical, and most preferred 95% identical; (e) a nucleic acid molecule which is degenerate with respect to the nucleic acid molecule of (b) or (d); or (f) a nucleic acid molecule corresponding to the nucleic acid molecule of any one of (a) to (d) wherein T is replaced by U.
摘要翻译：我们已经通过分子克隆鉴定了源自丝蚕幼虫的蛋白酶，由于其可用于清创伤口的活性，所以被称为脱氨酶。描述了编码具有裂解纤维蛋白和酪蛋白的能力的丝氨酸蛋白酶的核酸分子，其是（a）编码包含或由SEQ ID NO：4的氨基酸序列组成的丝氨酸蛋白酶的核酸分子以及核酸编码所述丝氨酸蛋白酶的前体或片段的酸分子; （b）包含SEQ ID NO：3的核苷酸序列或由其组成的核酸分子; （c）编码丝氨酸蛋白酶的核酸分子，其氨基酸序列与（a）的氨基酸序列至少80％相同，优选至少85％相同，更优选至少90％相同，最多优选95％相同; （d）核酸分子，其包含与（b）的核苷酸序列至少80％相同的核苷酸序列或由其组成，优选至少85％相同，更优选至少90％相同，最优选95％相同; （e）相对于（b）或（d）的核酸分子简并的核酸分子; 或（f）对应于（a）至（d）中任一项的核酸分子的核酸分子，其中T被U.替代

8. 发明申请

WO2010060811A3 HYDROPHOBINS AS SURFACE ACTIVE PROTEINS AS EXCIPIENTS IN SOLID PHARMACEUTICAL FORMULATIONS 审中-公开
标题翻译：作为表面活性蛋白的水解物作为固体药物制剂中的主要成分
公开(公告)号：WO2010060811A3
公开(公告)日：2010-10-21
申请号：PCT/EP2009065100
申请日：2009-11-13
申请人： BASF SE , BUTHE ANDREAS , HAFNER ANDREAS , KAUFMANN FRANZ , GABOR ESTHER , MEURER GUIDO , ECK JUERGEN , BRADLEY GORDON
发明人： BUTHE ANDREAS , HAFNER ANDREAS , KAUFMANN FRANZ , GABOR ESTHER , MEURER GUIDO , ECK JUERGEN , BRADLEY GORDON
IPC分类号： A61K9/20 , A61K9/00 , A61K9/28 , C07K14/37
CPC分类号： A61K9/4891 , A61K9/0065 , A61K9/2063 , A61K9/2095 , A61K9/2873 , C07K14/37
摘要： The invention relates to a use of surface active hydrophobins for applications in pharmaceutical technology, in particular as excipients for galenic use. Provided is a method for either admixture of hydrophobins to galenic compositions or for treating the surface of pharmaceutical forms with a hydrophobin-containing solution to modify the pharmaceutical properties of the galenic form. In a preferred embodiment of the invention hydrophobins are used to improve the properties of a pharmaceutical composition, e. g. to act as a surfactant or to increase resistance to disintegration of the galenic forms to achieve a retarded drug release. The galenic form to be modified by the use of surface active proteins as excipients can be capsules, tablets, pills, microparticles, vesicles, and suppositories, although further galenic forms are envisioned. The surface active proteins used for the purpose of present invention can either be isolated from their respective natural source or prepared by recombinant techniques and expression in a suitable host.
摘要翻译：本发明涉及用于制药技术中的表面活性疏水蛋白的用途，特别是作为涂覆液使用的赋形剂。提供了将疏水蛋白与盖仑组合物混合或用含疏水蛋白的溶液处理药物形式的表面以改变盖仑膜形式的药物性质的方法。在本发明的优选实施方案中，疏水蛋白用于改善药物组合物的性质，例如， G。作为表面活性剂或增加耐受霜冻形式的崩解以实现延缓药物释放。通过使用表面活性蛋白质作为赋形剂来修饰的盖仑形式可以是胶囊，片剂，丸剂，微粒，囊泡和栓剂，尽管可以预见到更多的盖仑制剂形式。用于本发明目的的表面活性蛋白质可以从它们各自的天然来源中分离或者通过重组技术制备并在合适的宿主中表达。

9. 发明申请

WO2012028709A3 NOVEL MONOOXYGENASE VARIANTS 审中-公开
标题翻译：新的MONOOXYGENASE变体
公开(公告)号：WO2012028709A3
公开(公告)日：2012-04-26
申请号：PCT/EP2011065179
申请日：2011-09-02
申请人： BRAIN BIOTECHNOLOGY RES & INFORMATION NETWORK AG , SCHWANEBERG ULRICH , BLANUSA MILAN , NIEHAUS FRANK , ECK JUERGEN
发明人： SCHWANEBERG ULRICH , BLANUSA MILAN , NIEHAUS FRANK , ECK JUERGEN
IPC分类号： C12N9/02
CPC分类号： C12Y106/02004 , C12N9/0042
摘要： The present invention relates to a nucleic acid molecule encoding a polypeptide having cytochrome P450 monooxygenase activity, wherein said polypeptide comprises a reductase domain that deviates by at least one mutation from (a) the reductase domain of cytochrome P450 BM3, wherein the reductase domain of cytochrome P450 BM3 is represented by SEQ ID NO: 1; or (b) a reductase domain having at least 95% sequence identity to SEQ ID NO:1; and wherein said mutation(s) result(s) in an increased cytochrome P450 monooxygenase activity as compared to a polypeptide comprising the reductase domain of SEQ ID NO: 1. The present invention also relates to a vector comprising the nucleic acid molecule of the invention and a host transformed with the vector. Furthermore, the invention relates to a method of producing a polypeptide comprising culturing the host of the invention as well as to a polypeptide encoded by the nucleic acid molecule of the invention or produced by the method of the invention. The present invention further relates to the use of the polypeptide of the invention in biotransformation or fine chemical synthesis, to an oligo- or polynucleotide which specifically hybridizes to the nucleic acid molecule of the invention as well as to a composition and to a kit.
摘要翻译：本发明涉及编码具有细胞色素P450单加氧酶活性的多肽的核酸分子，其中所述多肽包含与（a）细胞色素P450 BM3的还原酶结构域至少一个突变偏差的还原酶结构域，其中细胞色素的还原酶结构域 P450 BM3由SEQ ID NO：1表示; 或（b）与SEQ ID NO：1具有至少95％序列同一性的还原酶结构域; 并且其中与包含SEQ ID NO：1的还原酶结构域的多肽相比，所述突变导致细胞色素P450单加氧酶活性增加。本发明还涉及包含本发明的核酸分子的载体和用载体转化的宿主。此外，本发明涉及生产多肽的方法，其包括培养本发明的宿主以及由本发明的核酸分子编码的多肽或通过本发明的方法产生的多肽。本发明进一步涉及本发明的多肽在生物转化或精细化学合成中的用途，涉及与本发明的核酸分子以及组合物和试剂盒特异性杂交的寡核苷酸或多核苷酸。

10. 发明申请

WO2011057710A2 MICROORGANISMS HAVING ENHANCED SUCROSE MUTASE ACTIVITY 审中-公开
标题翻译：增加SUCROSEMUTASEAKTIVITÄT微生物
公开(公告)号：WO2011057710A2
公开(公告)日：2011-05-19
申请号：PCT/EP2010006487
申请日：2010-10-23
申请人： SUEDZUCKER AG , PELZER STEFAN , ZUREK CHRISTIAN , ROSE THOMAS , ECK JUERGEN , WACH WOLFGANG , KLINGEBERG MICHAEL , HARMS KARSTEN
发明人： PELZER STEFAN , ZUREK CHRISTIAN , ROSE THOMAS , ECK JUERGEN , WACH WOLFGANG , KLINGEBERG MICHAEL , HARMS KARSTEN
IPC分类号： C12P19/12
CPC分类号： C12N9/90 , C12P19/12 , C12P19/24 , C12Y504/99011
摘要： The invention relates to the biotechnological production of isomaltulose and isomaltulose-containing compositions and to improved agents, in particular microbial cells, for said purpose.
摘要翻译：本发明涉及生物技术生产异麦芽酮糖和异麦芽酮糖含有的组合物和改进的方法，特别是微生物细胞。

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