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1. 发明申请

WO2004018673A3 ISOLATION AND CLONING OF DNA FROM UNCULTIVATED ORGANISMS 审中-公开
标题翻译：分离和克隆来自不同组织的DNA
公开(公告)号：WO2004018673A3
公开(公告)日：2004-08-12
申请号：PCT/EP0309223
申请日：2003-08-20
申请人： B R A I N BIOTECHNOLOGY RES AN , QUAISER ACHIM , OCHSENREITER TORSTEN , TREUSCH ALEXANDER H , KLETZIN ARNULF , SCHLEPER CHRISTA , LORENZ PATRICK , ECK JUERGEN
发明人： QUAISER ACHIM , OCHSENREITER TORSTEN , TREUSCH ALEXANDER H , KLETZIN ARNULF , SCHLEPER CHRISTA , LORENZ PATRICK , ECK JUERGEN
IPC分类号： C12N15/10
CPC分类号： C12N15/1003
摘要： The present invention relates to a device for the isolation and/or purification of nucleic acid molecules suitable to bind and/or inactivate inhibitors of the activity of reagents or enzymes used for DNA manipulation and to separate a plurality of nucleic acid molecules with respect to their size. Moreover, the invention relates to a method for the isolation of a nucleic acid molecule comprising applying a sample to the device of the invention wherein said nucleic acid molecule preferably represents a fraction of the metagenome of a given habitat. Furthermore, the invention relates to a method for the generation of at least one gene library comprising nucleic acid molecules isolated by the method of the invention and to a nucleic acid molecule isolated by the method of the invention.
摘要翻译：本发明涉及用于分离和/或纯化适于结合和/或失活用于DNA操作的试剂或酶的活性抑制剂的核酸分子的装置，并且相对于它们分离多个核酸分子尺寸。此外，本发明涉及用于分离核酸分子的方法，包括将样品施加到本发明的装置中，其中所述核酸分子优选代表给定栖息地的元素的一部分。此外，本发明涉及产生至少一种包含通过本发明方法分离的核酸分子的基因文库以及通过本发明的方法分离的核酸分子的方法。

2. 发明专利

IL166979A DEVICE FOR ISOLATION AND CLONING OF DNA FROM UNCULTIVATED ORGANISMS 未知
公开(公告)号：IL166979A
公开(公告)日：2012-04-30
申请号：IL16697905
申请日：2005-02-17
申请人： BRAIN BIOTECHNOLOGY RES & INFORMATION NETWORK AG , QUAISER ACHIM , OCHSENREITER TORSTEN , TREUSCH ALXANDER H , KLETZIN ARNULF , SCHLEPER CHRISTA , LORENZ PATRICK , ECK JUERGEN
发明人： QUAISER ACHIM , OCHSENREITER TORSTEN , TREUSCH ALXANDER H , KLETZIN ARNULF , SCHLEPER CHRISTA , LORENZ PATRICK , ECK JUERGEN
IPC分类号： C12N15/10
摘要： The present invention relates to a device for the isolation and/or purification of nucleic acid molecules suitable to bind and/or inactivate inhibitors of the activity of reagents or enzymes used for DNA manipulation and to separate a plurality of nucleic acid molecules with respect to their size. Moreover, the invention relates to a method for the isolation of a nucleic acid molecule comprising applying a sample to the device of the invention wherein said nucleic acid molecule preferably represents a fraction of the metagenome of a given habitat. Furthermore, the invention relates to a method for the generation of at least one gene library comprising nucleic acid molecules isolated by the method of the invention and to a nucleic acid molecule isolated by the method of the invention.

3. 发明申请

WO2004106527B1 ARCHAEON EXPRESSION SYSTEM 审中-公开
标题翻译： ARCHAEON表达系统
公开(公告)号：WO2004106527B1
公开(公告)日：2005-01-20
申请号：PCT/EP2004005936
申请日：2004-06-02
申请人： B R A I N BIOTECHNOLOGY RES AN , SCHLEPER CHRISTA , JONUSCHEIT MELANIE , ECK JUERGEN , NIEHAUS FRANK , ALBERS SONJA-VERENA , FROELS SABRINA
发明人： SCHLEPER CHRISTA , JONUSCHEIT MELANIE , ECK JUERGEN , NIEHAUS FRANK , ALBERS SONJA-VERENA , FROELS SABRINA
IPC分类号： C12N15/74
CPC分类号： C12N15/74
摘要： The present invention relates to a sulfolobus expression vector comprising: (a) sulfolobus origin of replication; (b) the genes encoding the structural proteins and the site-specific integrase of SSVI, SSV2 or pSSVx, operatively linked to expression control sequences and a packaging signal; (c) one or more selectable marker gene(s), operatively linked to sulfolobus expression control sequences; and (d) a sulfolobus promoter followed 3' by a restriction enzyme recognition site or a multiple cloning site for insertion of a gene of interest and optionally a 3' regulatory element. Moreover, the present invention relates to a shuttle vector comprising the sequences of the expression vector of the invention and additional sequences for propagation and selection in E. coli, wherein the additional sequences comprise (a) an E.coli on of replication; and (b) a marker for selection in E.coli. Furthermore, the invention relates to host cells transformed with the expression vector as well as to a kit comprising a vector or a host cell of the present invention. Finally, the present application also relates to a method for generating infectious subviral particles.
摘要翻译：本发明涉及一种磺叶病毒表达载体，其包含：（a）磺叶酸复制起点; （b）编码与表达控制序列和包装信号有效连接的结构蛋白和SSVI，SSV2或pSSVx的位点特异性整合酶的基因; （c）一个或多个可选择的标记基因，其可操作地连接到子宫颈表达控制序列; 和（d）通过限制性酶识别位点或用于插入目的基因和任选的3'调节元件的多克隆位点的3'端的磺基叶绿体启动子。此外，本发明涉及包含本发明的表达载体的序列和在大肠杆菌中增殖和选择的其它序列的穿梭载体，其中所述另外的序列包含（a）复制大肠杆菌; 和（b）在大肠杆菌中选择的标记物。此外，本发明涉及用表达载体转化的宿主细胞以及包含本发明的载体或宿主细胞的试剂盒。最后，本申请还涉及产生感染性亚病毒颗粒的方法。

4. 发明专利

AT481493T 未知
公开(公告)号：AT481493T
公开(公告)日：2010-10-15
申请号：AT04739516
申请日：2004-06-02
申请人： B R A I N BIOTECHNOLOGY RES AN
发明人： SCHLEPER CHRISTA , JONUSCHEIT MELANIE , ECK JUERGEN , NIEHAUS FRANK , ALBERS SONJA-VERENA , FROELS SABRINA
IPC分类号： C12N15/74
摘要： The present invention relates to a sulfolobus expression vector comprising: (a) sulfolobus origin of replication; (b) the genes encoding the structural proteins and the site-specific integrase of SSV1, SSV2 or pSSVx, operatively linked to expression control sequences and a packaging signal; (c) one or more selectable marker gene(s), operatively linked to sulfolobus expression control sequences; and (d) a sulfolobus promoter followed 3′ by a restriction enzyme recognition site or a multiple cloning site for insertion of a gene of interest and optionally a 3′ regulatory element. Moreover, the present invention relates to a shuttle vector comprising the sequences of the expression vector of the invention and additional sequences for propagation and selection in E. coli, wherein the additional sequences comprise (a) an E. coli on of replication; and (b) a marker for selection in E. coli. Furthermore, the invention relates to host cells transformed with the expression vector as well as to a kit comprising a vector or a host cell of the present invention. Finally, the present application also relates to a method for generating infectious subviral particles.

5. 发明专利

DK1530633T3 Isolering og kloning af DNA fra ikke-dyrkede organismer 未知
公开(公告)号：DK1530633T3
公开(公告)日：2010-09-27
申请号：DK03792391
申请日：2003-08-20
申请人： B R A I N BIOTECHNOLOGY RES AN
发明人： TREUSCH ALEXANDER H , KLETZIN ARNULF , LORENZ PATRICK , SCHLEPER CHRISTA , ECK JUERGEN , QUAISER ACHIM , OCHSENREITER TORSTEN
IPC分类号： C12N15/10

6. 发明专利

CA2495797A1 ISOLATION AND CLONING OF DNA FROM UNCULTIVATED ORGANISMS 未知
公开(公告)号：CA2495797A1
公开(公告)日：2004-03-04
申请号：CA2495797
申请日：2003-08-20
申请人： B R A I N BIOTECHNOLOGY RES AN
发明人： LORENZ PATRICK , QUAISER ACHIM , SCHLEPER CHRISTA , TREUSCH ALEXANDER H , KLETZIN ARNULF , OCHSENREITER TORSTEN , ECK JURGEN
IPC分类号： C12N15/10
摘要： The present invention relates to a device for the isolation and/or purification of nucleic acid molecules suitable to bind and/or inactivate inhibitors of the activity of reagents or enzymes used for DNA manipulation and to separate a plurality of nucleic acid molecules with respect to their size. Moreover, the invention relates to a method for the isolation of a nucleic acid molecule comprising applying a sample to the device of the invention wherein said nucleic acid molecule preferably represents a fractio n of the metagenome of a given habitat. Furthermore, the invention relates to a method for the generation of at least one gene library comprising nucleic ac id molecules isolated by the method of the invention and to a nucleic acid molecule isolated by the method of the invention.

7. 发明专利

AT471981T 未知
公开(公告)号：AT471981T
公开(公告)日：2010-07-15
申请号：AT03792391
申请日：2003-08-20
申请人： B R A I N BIOTECHNOLOGY RES AN
发明人： QUAISER ACHIM , OCHSENREITER TORSTEN , TREUSCH ALEXANDER H , KLETZIN ARNULF , SCHLEPER CHRISTA , LORENZ PATRICK , ECK JUERGEN
IPC分类号： C12N15/10
摘要： The present invention relates to a device for the isolation and/or purification of nucleic acid molecules suitable to bind and/or inactivate inhibitors of the activity of reagents or enzymes used for DNA manipulation and to separate a plurality of nucleic acid molecules with respect to their size. Moreover, the invention relates to a method for the isolation of a nucleic acid molecule comprising applying a sample to the device of the invention wherein said nucleic acid molecule preferably represents a fraction of the metagenome of a given habitat. Furthermore, the invention relates to a method for the generation of at least one gene library comprising nucleic acid molecules isolated by the method of the invention and to a nucleic acid molecule isolated by the method of the invention.

8. 发明专利

DE60333101D1 IERTEN ORGANISMEN 未知
公开(公告)号：DE60333101D1
公开(公告)日：2010-08-05
申请号：DE60333101
申请日：2003-08-20
申请人： B R A I N BIOTECHNOLOGY RES AN
发明人： QUAISER ACHIM , OCHSENREITER TORSTEN , TREUSCH ALEXANDER H , KLETZIN ARNULF , SCHLEPER CHRISTA , LORENZ PATRICK , ECK JUERGEN
IPC分类号： C12N15/10

9. 发明专利

CA2527704A1 ARCHAEON EXPRESSION SYSTEM 未知
公开(公告)号：CA2527704A1
公开(公告)日：2004-12-09
申请号：CA2527704
申请日：2004-06-02
申请人： B R A I N BIOTECHNOLOGY RES AN
发明人： NIEHAUS FRANK , ECK JUERGEN , ALBERS SONJA-VERENA , JONUSCHEIT MELANIE , FROELS SABRINA , SCHLEPER CHRISTA
IPC分类号： C12N15/74
摘要： The present invention relates to a sulfolobus expression vector comprising: (a) sulfolobus origin of replication; (b) the genes encoding the structural proteins and the site-specific integrase of SSVI, SSV2 or pSSVx, operatively linked to expression control sequences and a packaging signal; (c) one or mo re selectable marker gene(s), operatively linked to sulfolobus expression contr ol sequences; and (d) a sulfolobus promoter followed 3' by a restriction enzyme recognition site or a multiple cloning site for insertion of a gene of interest and optionally a 3' regulatory element. Moreover, the present invention relates to a shuttle vector comprising the sequences of the expression vector of the invention and additional sequences for propagation and selection in E. coli, wherein the additional sequences comprise (a) an E.coli on of replication; and (b) a marker for selection in E.coli. Furthermore, the invention relates to host cells transformed with the expression vector as well as to a kit comprising a vector or a host cell of the present invention. Finally, the present application also relates to a method for generating infectious subviral particles.

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