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    • 10. 发明申请
    • CELL ARRAY OR MATRIX ASSEMBLY AND ELECTROPORATION
    • 细胞阵列或基质组件和电极
    • US20100056396A1
    • 2010-03-04
    • US12515985
    • 2007-11-28
    • Dirkjan Bernhard Van DamSimone Irene Elisabeth VultoThomas Jan De HoogJudith Margreet Rensen
    • Dirkjan Bernhard Van DamSimone Irene Elisabeth VultoThomas Jan De HoogJudith Margreet Rensen
    • C40B50/14C40B60/14
    • C12M35/02
    • The present invention relates to an apparatus 1 for transferring cells 2 suspended in a fluid 3 from the fluid 3 to a substrate 17. The apparatus 1 comprises a transfer device 8 having an exterior surface 9, an interior cavity 10, and a plurality of passages 11 each having a first end 12 at the exterior surface 9 and a second end 13 in fluid 5 communication with the cavity 10 and each having a diameter corresponding to a predefined size of the cells 2 to be transferred. The apparatus further comprises a pressure regulating device 14 capable of regulating a pressure in the cavity 10 so as to establish a pressure difference between the first and second ends 12, 13 of each passage 11, a container 4 for containing the fluid 3, and a flow device 7 capable of causing the fluid 3 to flow across the 10 exterior surface 9 when in contact with the fluid 3. By appropriate selection of passage diameter, pressure difference and fluid flow velocity, the transfer device can be used to pick up and transfer cells 2 within a predefined size range. The invention further relates to a method for transferring cells 2 by use of such an apparatus 1.
    • 本发明涉及一种用于将悬浮在流体3中的细胞2从流体3转移到基底17的装置1.装置1包括具有外表面9,内腔10和多个通道的转移装置8 11,每个具有在外表面9处的第一端12和流体5中的与空腔10连通的第二端13,每个具有对应于要传送的单元2的预定尺寸的直径。 该装置还包括压力调节装置14,其能够调节空腔10中的压力,以便建立每个通道11的第一和第二端12,13之间的压力差,容纳流体3的容器4和 流体装置7能够使流体3在与流体3接触时流过10个外表面9.通过适当选择通道直径,压力差和流体流速,转移装置可用于拾取和转移 小区2在预定义的大小范围内。 本发明还涉及使用这种装置1来传送电池2的方法。