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2. 发明公开

EP0718628A1 Method for diagnosis of blood coagulation disorders 失效
标题翻译： Verfahren zur诊断vonBlutgerinnungsstörungen
公开(公告)号：EP0718628A1
公开(公告)日：1996-06-26
申请号：EP95304016.9
申请日：1995-06-09
申请人： Diagnostic Reagents Limited
发明人： Denson, Kenneth William Ernest
IPC分类号： G01N33/86 , C12Q1/56
CPC分类号： C12Q1/56 , G01N2333/462 , G01N2333/745 , G01N2333/96461
摘要： A method and kit for screening blood plasma for APC cofactor is provided. The method comprises:

(i) incubating a first portion of the sample with

(a) an exogenous reagent containing phospholipid which activates the contact mechanism in intrinsic clotting or an exogenous reagent which initiates extrinsic clotting; and incubating a second portion of the sample with
(b) the reagent of step (i) (a) and an exogenous Protein C (PC) activator which produces activated PC (APC) from endogenous Protein C and Protein S.

(ii) adding to each of the two portions a clotting agent and monitoring the conversion rate of fibrinogen to insoluble fibrin; and
(iii) calculating the ratio of the rate for the second portion of the sample to the rate for the first portion and comparing this ratio with the corresponding ratio for samples from normal subjects.
摘要翻译：提供了用于APC辅助因子筛选血浆的方法和试剂盒。该方法包括：（i）将样品的第一部分与（a）含有磷脂的外源试剂孵育，所述磷脂激活内在凝血中的接触机制或引发外在凝血的外源试剂; 和（b）步骤（i）（a）的试剂和从内源蛋白C和蛋白S产生活化的PC（APC）的外源蛋白C（PC）活化剂孵育第二部分样品。（ii）加入将两个部分中的每一个分成凝血剂并监测纤维蛋白原转化为不溶性纤维蛋白; 和（iii）计算样品的第二部分的速率与第一部分的比率的比率，并将该比率与来自正常受试者的样品的相应比率进行比较。

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