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1. 发明授权

US09028825B2 Diagnostic method for brain damage-related disorders 有权
标题翻译：脑损伤相关疾病的诊断方法
公开(公告)号：US09028825B2
公开(公告)日：2015-05-12
申请号：US11995501
申请日：2006-07-14
申请人： Denis Francois Hochstrasser , Jean-Charles Sanchez
发明人： Denis Francois Hochstrasser , Jean-Charles Sanchez
IPC分类号： A61K39/395 , C07K16/40 , G01N33/68
CPC分类号： G01N33/6896 , G01N33/6893 , G01N2800/28 , G01N2800/30
摘要： A brain damage-related disorder is diagnosed in a subject by detecting at least one polypeptide, or a variant or mutant thereof, in a sample of body fluid taken from the subject, wherein the polypeptide is one for which the level is either increased or decreased in cerebrospinal fluid from deceased patients compared to cerebrospinal fluid from healthy donors.
摘要翻译：通过检测取自受试者的体液样品中的至少一种多肽或其变体或突变体，在受试者中诊断脑损伤相关疾病，其中所述多肽是水平增加或减少的多肽来自死亡患者的脑脊液与来自健康供体的脑脊液相比。

2. 发明授权

US08772042B2 Biomarkers for diagnosing alzheimer's disease 有权
标题翻译：用于诊断阿尔茨海默病的生物标志物
公开(公告)号：US08772042B2
公开(公告)日：2014-07-08
申请号：US13099110
申请日：2011-05-02
申请人： Özkan Yalkinoglu , Gerhard König , Denis Francois Hochstrasser , Jean-Charles Sanchez , Odile Carrette
发明人： Özkan Yalkinoglu , Gerhard König , Denis Francois Hochstrasser , Jean-Charles Sanchez , Odile Carrette
IPC分类号： G01N33/00
CPC分类号： G01N33/6896 , C07K7/08 , C07K14/70539 , C07K14/805 , C07K14/8139 , G01N2800/2821
摘要： A method for assessing the state of Alzheimer's disease in patients is disclosed. A method for monitoring the progression of Alzheimer's disease in patients is also disclosed. The method applies detection of specific peptide markers, e.g., using mass spectrometric analysis.
摘要翻译：公开了一种评估患者阿尔茨海默病状态的方法。还公开了一种用于监测患者中阿尔茨海默病进展的方法。该方法应用特异性肽标志物的检测，例如使用质谱分析。

3. 发明授权

US06632339B1 Method of identifying polypeptides 失效
标题翻译：鉴定多肽的方法
公开(公告)号：US06632339B1
公开(公告)日：2003-10-14
申请号：US09594906
申请日：2000-06-15
申请人： Willy Vincent Bienvenut , Denis Francois Hochstrasser
发明人： Willy Vincent Bienvenut , Denis Francois Hochstrasser
IPC分类号： B01D5702
CPC分类号： C12Q1/37 , Y10S435/81
摘要： Polypeptides which have been separated by gel electrophoresis can be identified by electroblotting them through a “sandwich” comprising in order: a) the separation gel; b) at least one hydrophilic membrane, e.g. of carboxyl-modified PVDF, on which is immobilized at least one reagent capable of cleaving a polypeptide, e.g. trypsin; c) a hydrophobic layer, typically a membrane, e.g. of PVDF. Preferably a biased alternating current or discontinuous direct current is used for electroblotting. The resulting fragments, usually peptides, are identified, preferably by MALDI-TOF MS.
摘要翻译：已经通过凝胶电泳分离的多肽可以通过电渗透它们通过包含以下顺序的“夹心”来鉴定：a）分离凝胶; b）至少一个亲水膜，例如，的羧基改性的PVDF，其上固定有至少一种能够切割多肽的试剂，例如。胰蛋白酶; c）疏水层，通常为膜，例如。的PVDF。优选地，偏置的交流电流或不连续的直流电用于电印迹。所得到的片段，通常是肽，优选通过MALDI-TOF MS鉴定

4. 发明授权

US07754436B2 Diagnostic assay for stroke 有权
标题翻译：中风诊断分析
公开(公告)号：US07754436B2
公开(公告)日：2010-07-13
申请号：US11536283
申请日：2006-09-28
申请人： Denis Francois Hochstrasser , Jean-Charles Sanchez , Catherine Gabrielle Zimmerman
发明人： Denis Francois Hochstrasser , Jean-Charles Sanchez , Catherine Gabrielle Zimmerman
IPC分类号： G01N33/53 , G01N33/537
CPC分类号： G01N33/6893 , G01N2800/2871 , G01N2800/324 , Y10S435/97 , Y10S435/973 , Y10S436/811 , Y10T436/104165 , Y10T436/105831 , Y10T436/106664
摘要： Heart and brain fatty acid binding proteins (H-FABP, B-FABP) are markers for stroke. The invention provides a diagnostic assay for either of these markers, preferably by ELISA using a anti-H-FABP or B-FABP antibody. Since H-FABP is also a marker for acute myocardial infarction (AMI), to distinguish stroke from AMI requires an assay specific to AMI, e.g. using troponin-1 or CK-MB as a marker, also to be carried out.
摘要翻译：心脏和脑脂肪酸结合蛋白（H-FABP，B-FABP）是中风的标志物。本发明提供了用于这些标记之一的诊断测定法，优选通过使用抗H-FABP或B-FABP抗体的ELISA进行。由于H-FABP也是急性心肌梗塞（AMI）的标志物，因此要区分中风与AMI需要AMI特异性检测。使用肌钙蛋白-1或CK-MB作为标记，也要进行。

5. 发明授权

US07144705B2 Diagnostic assay for stroke 有权
公开(公告)号：US07144705B2
公开(公告)日：2006-12-05
申请号：US10165127
申请日：2002-06-07
申请人： Denis Francois Hochstrasser , Jean-Charles Sanchez , Catherine Gabrielle Zimmerman
发明人： Denis Francois Hochstrasser , Jean-Charles Sanchez , Catherine Gabrielle Zimmerman
IPC分类号： G01N33/53 , G01N33/537
CPC分类号： G01N33/6893 , G01N2800/2871 , G01N2800/324 , Y10S435/97 , Y10S435/973 , Y10S436/811 , Y10T436/104165 , Y10T436/105831 , Y10T436/106664
摘要： Heart and brain fatty acid binding proteins (H-FABP, B-FABP) are markers for stroke. The invention provides a diagnostic assay for either of these markers, preferably by ELISA using anti-H-FABP or B-FABP antibody. Since H-FABP is also a marker for acute myocardial infarction (AMI), to distinguish stroke from AMI requires an assay specific to AMI, e.g. using troponin-I or creatine kinase-MB as a marker, also to be carried out.

6. 发明申请

US20120016205A1 BIOMARKERS FOR DIAGNOSING ALZHEIMER'S DISEASE 有权
标题翻译：用于诊断阿尔茨海默病的生物标志物
公开(公告)号：US20120016205A1
公开(公告)日：2012-01-19
申请号：US13099110
申请日：2011-05-02
申请人： Õzkan Yalkinoglu , Gerhard Kõnig , Denis Francois Hochstrasser , Jean-Charles Sanchez , Odile Carrette
发明人： Õzkan Yalkinoglu , Gerhard Kõnig , Denis Francois Hochstrasser , Jean-Charles Sanchez , Odile Carrette
IPC分类号： A61B5/00
CPC分类号： G01N33/6896 , C07K7/08 , C07K14/70539 , C07K14/805 , C07K14/8139 , G01N2800/2821
摘要： A method for assessing the state of Alzheimer's disease in patients is disclosed. A method for monitoring the progression of Alzheimer's disease in patients is also disclosed. The method applies detection of specific peptide markers, e.g., using mass spectrometric analysis.
摘要翻译：公开了一种评估患者阿尔茨海默病状态的方法。还公开了一种用于监测患者中阿尔茨海默病进展的方法。该方法应用特异性肽标志物的检测，例如使用质谱分析。

7. 发明授权

US07767401B2 Diagnostic method for stroke 有权
标题翻译：中风诊断方法
公开(公告)号：US07767401B2
公开(公告)日：2010-08-03
申请号：US10568093
申请日：2004-08-16
申请人： Pierre Lescuyer , Denis Francois Hochstrasser , Jean-Charles Sanchez , Laure Allard , Elisabeth Guillaume
发明人： Pierre Lescuyer , Denis Francois Hochstrasser , Jean-Charles Sanchez , Laure Allard , Elisabeth Guillaume
IPC分类号： G01N33/00 , G01N33/543
CPC分类号： G01N33/6893 , G01N2333/4709 , G01N2333/775 , G01N2333/8128 , G01N2800/2871
摘要： Stroke is diagnosed in a subject by determining the concentration of at least one polypeptide selected from Apo C-III, Serum Amyloid A, Apo C-I, Antithrombin III fragment and Apo A-I in a sample of body fluid taken from the subject.
摘要翻译：通过从取自受试者的体液样品中测定至少一种选自Apo C-III，血清淀粉样蛋白A，Apo C-I，抗凝血酶III片段和Apo A-I的多肽的浓度，在受试者中诊断中风。

8. 发明授权

US07368247B2 Diagnostic assay for transmissible spongiform encephalopathies 有权
标题翻译：传染性海绵状脑病的诊断测定
公开(公告)号：US07368247B2
公开(公告)日：2008-05-06
申请号：US10238557
申请日：2002-09-10
申请人： Denis Francois Hochstrasser , Jean-Charles Sanchez , Catherine Gabrielle Zimmermann , Elisabeth Guillaume
发明人： Denis Francois Hochstrasser , Jean-Charles Sanchez , Catherine Gabrielle Zimmermann , Elisabeth Guillaume
IPC分类号： G01N33/53
CPC分类号： G01N33/6896 , G01N2800/2828 , Y10S436/811 , Y10S436/819 , Y10T436/106664
摘要： Heart and brain fatty acid binding proteins (H-FABP, B-FABP) are markers for TSEs, especially CJD. The invention provides a diagnostic assay for either of these markers, preferably by enzyme immunoassay using a specific antibody thereto. Since H-FABP is also a marker for acute myocardial infarction (AMI), to distinguish CJD from AMI requires an assay specific to AMI, e.g. using troponin-1 or CK-MB as a marker, also to be carried out.
摘要翻译：心脏和脑脂肪酸结合蛋白（H-FABP，B-FABP）是TSEs的标志物，特别是CJD。本发明提供了对这些标记之一的诊断测定，优选通过使用其特异性抗体的酶免疫测定。由于H-FABP也是急性心肌梗死（AMI）的标志物，因此区分CJD与AMI需要AMI特异性检测，例如使用肌钙蛋白-1或CK-MB作为标记，也要进行。

9. 发明授权

US06787330B1 Method and kit for identifying or characterizing polypeptides 失效
标题翻译：用于鉴定或表征多肽的方法和试剂盒
公开(公告)号：US06787330B1
公开(公告)日：2004-09-07
申请号：US09889711
申请日：2001-07-18
申请人： Willy Vincent Bienvenut , Denis Francois Hochstrasser , Jean-Charles Sanchez
发明人： Willy Vincent Bienvenut , Denis Francois Hochstrasser , Jean-Charles Sanchez
IPC分类号： C12Q137
CPC分类号： G01N33/6848 , G01N33/525 , G01N33/6803 , G01N33/6851 , Y10S435/81
摘要： Polypeptides which have been separated by gel electrophoresis can be identified or characterized by a procedure which has two main stages. In the first stage the gel is digested with a polypeptide-cleaving agent such as an enzyme. This produces mainly large fragments which, in the second stage are electroblotted through a hydrophilic membrane on which is immobilized another polypeptide-cleaving reagent such as an enzyme onto a hydrophobic member, typically a membrane, e.g. of PVDF. The resulting fragments, usually peptides, are identified, preferably by MALDI-TOF MS, or a property may be determined, e.g. by interaction with an antibody.
摘要翻译：通过凝胶电泳分离的多肽可以通过具有两个主要阶段的程序来鉴定或表征。在第一阶段，用多肽切割剂如酶消化凝胶。这主要产生大的片段，其在第二阶段通过亲水膜电泳，其上固定有另一种多肽切割试剂如酶到疏水性成员上，通常为膜，例如膜。的PVDF。所得到的片段，通常是肽，优选通过MALDI-TOF MS鉴定，或者可以确定，例如，通过与抗体的相互作用。

10. 发明授权

US06221626B1 Kit for electroblotting polypeptides separated on an electrophoresis gel 失效
标题翻译：用于在电泳凝胶上分离的电印迹多肽的试剂盒
公开(公告)号：US06221626B1
公开(公告)日：2001-04-24
申请号：US09107991
申请日：1998-06-30
申请人： Willy Vincent Bienvenut , Denis Francois Hochstrasser
发明人： Willy Vincent Bienvenut , Denis Francois Hochstrasser
IPC分类号： C12Q137
CPC分类号： C12Q1/37 , Y10S435/81
摘要： Polypeptides separated on a gel are identified by cleaving the separated polypeptides with an immobilized cleaving reagent such as an enzyme, and transferring the fragments to a hydrophobic collection layer where they are analyzed. A hydrophilic membrane containing an immobilized protease such as trypsin is provided between an electrophoresis gel and a hydrophobic membrane to form an electroblotting “sandwich”. Polypeptides separated on the gel by electrophoresis are electroblotted from the gel through the hydrophilic membrane where they are cleaved by the protease into fragments, and the fragments are collected on the hydrophobic membrane where they are identified such as by MALDI-TOF MS analysis. From identification of the fragments, the polypeptide from which they came is identified. The hydrophilic membrane may be provided with functional groups to which the protease is immobilized by covalent bonding. Free residual functional groups not bound to the protease are capped to prevent their reaction with the polypeptide. The functional groups may be activated carbonyl groups, carboxylic acid groups or carboxylic acid derivative groups capable of reacting with an amino group of an enzyme. A kit for use in carrying out the electroblotting is formed containing the cleaving reagent immobilized on the hydrophilic membrane, and the hydrophobic collection layer. The immobilized cleaving reagent and hydrophobic layer may be in separate containers or in the same container.
摘要翻译：通过用固定化的切割试剂如酶切割分离的多肽来鉴定在凝胶上分离的多肽，并将片段转移到疏水性收集层，在那里进行分析。在电泳凝胶和疏水膜之间提供含有固定化蛋白酶如胰蛋白酶的亲水膜以形成电印迹“三明治”。通过电泳在凝胶上分离的多肽通过亲水膜从凝胶电泳印迹，在那里它们被蛋白酶切割成片段，并将片段收集在疏水膜上，例如通过MALDI-TOF MS分析。从鉴定片段中鉴定出来自其的多肽。亲水膜可以具有通过共价键固定蛋白酶的官能团。不结合蛋白酶的游离残基官能团被封端以防止其与多肽的反应。官能团可以是能够与酶的氨基反应的活化的羰基，羧酸基团或羧酸衍生物基团。形成用于进行电印迹的试剂盒，其包含固定在亲水膜上的裂解试剂和疏水性收集层。固定的切割试剂和疏水层可以在分开的容器中或在相同的容器中。

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