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1. 发明申请

WO2004072280A1 METHOD FOR THE PRODUCTION OF CHITIN DEACETYLASE 审中-公开
标题翻译：生产脱乙酰壳多糖的方法
公开(公告)号：WO2004072280A1
公开(公告)日：2004-08-26
申请号：PCT/EP2004/001313
申请日：2004-02-12
申请人： KITOZYME S.A. , UNIVERSITE DE LIEGE , VERSALI, Marie-France , CLERISSE, Fabienne , GAUTHIER, Carole , DUMONT, Françoise , DOMMES, Jacques
发明人： VERSALI, Marie-France , CLERISSE, Fabienne , GAUTHIER, Carole , DUMONT, Françoise , DOMMES, Jacques
IPC分类号： C12N9/80
CPC分类号： C12N1/16 , C07K2319/21 , C12N9/80 , C12Y305/01041
摘要： In a first aspect, the present invention relates to a recombinant fungal strain, capable of expressing chitin deacetylase, comprising an expression vector that contains a nucleic acid molecule encoding chitin deacetylase, a suitable promoter and a transcription terminator. In particular, the present invention provides a recombinant Aspergillus oryzae strain capable of expressing chitin deacetylase obtained from Mucor rouxii. The present invention also relates to a method for producing chitin deacetylase by a recombinant fungal strain. In a second aspect, the present invention relates to a recombinant yeast strain, capable of expressing chitin deacetylase, comprising an expression vector that contains a nucleic acid molecule encoding chitin deacetylase, a suitable promoter and a transcription terminator. In particular, the present invention provides a recombinant Pichia pastoris strain capable of expressing chitin deacetylase obtained from Mucor rouxii. The present invention also relates to a method for producing chitin deacetylase by a recombinant yeast strain. The present invention further relates to purified recombinant chitin deacetylase enzyme.
摘要翻译：在第一方面，本发明涉及能够表达几丁质脱乙酰酶的重组真菌菌株，其包含含有编码甲壳素脱乙酰酶的核酸分子，合适的启动子和转录终止子的表达载体。特别地，本发明提供了能够表达从毛霉（Mucor rouxii）获得的几丁质脱乙酰酶的重组米曲霉菌株。本发明还涉及通过重组真菌菌株生产壳多糖脱乙酰酶的方法。在第二方面，本发明涉及能够表达几丁质脱乙酰酶的重组酵母菌株，其包含含有编码甲壳素脱乙酰酶的核酸分子，合适的启动子和转录终止子的表达载体。特别地，本发明提供能够表达从毛霉（Mucor rouxii）获得的几丁质脱乙酰酶的重组毕赤酵母菌株。本发明还涉及通过重组酵母菌株生产壳多糖脱乙酰酶的方法。本发明还涉及纯化的重组壳多糖脱乙酰酶。

2. 发明申请

WO1994009729A1 KNEE SUPPORT DEVICE OR PROSTHESIS 审中-公开
标题翻译： KNEE支持设备或PROSTHESIS
公开(公告)号：WO1994009729A1
公开(公告)日：1994-05-11
申请号：PCT/BE1993000066
申请日：1993-10-22
申请人： DUMONT, Françoise, Ghislaine , POSTELMANS, Roberto, Jean, José
发明人： DUMONT, Françoise, Ghislaine
IPC分类号： A61F05/01
CPC分类号： A61F5/0123 , A61F2005/0146
摘要： Knee support device or prosthesis of the asymmetrical multicircumvolution type comprising at least one external articulation and one internal articulation disposed on either side of a front to back vertical plane, each including at least one proximal portion (7, 19) and at least one distal portion (1, 13) articulated to one another, first connecting means (25, 26) for connecting the proximal portions to distal portions and guiding means (4, 5, 10, 11, 16, 17, 22, 23) allowing the articulated portions to effect a different movement for each articulation. The articulated portions of each articulation have bearing surfaces with complex curves different from one another, whereby they are only in partial contact at at least three points.
摘要翻译：包括至少一个外部关节和一个内部关节的膝关节支撑装置或假体，其包括设置在前后垂直平面的任一侧的每个包括至少一个近端部分（7,19）和至少一个远端部分（1,13）彼此铰接，用于将近端部分连接到远端部分的第一连接装置（25,26）和引导装置（4,5,10,11,16,17,22,23）允许铰接部分对每个关节进行不同的运动。每个关节的铰接部分具有彼此不同的复杂曲线的承载表面，由此它们在至少三个点处仅部分接触。

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