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1. 发明专利

IL228849D0 Synthetic genes 未知
公开(公告)号：IL228849D0
公开(公告)日：2013-12-31
申请号：IL22884913
申请日：2013-10-13
申请人： DOW AGROSCIENCES LLC , DONALD J MERLO , AVUTU S REDDY , ARVIND KUMAR THIRUMALAISWAMYSEKHAR , IGNACIO MARIO LARRINUA , AARON TODD WOOSLEY
IPC分类号： A23L7/10 , A23L25/00
摘要： The invention provides synthetic nucleic acid sequences encoding proteins of interest that are particularly adapted to express well in plants. The claimed synthetic sequences utilize plant-optimized codons roughly in the same frequency at which they are utilized, on average, in genes naturally occurring in the plant species. The invention further includes synthetic DNA sequence for herbicide tolerance, water and/or heat stress tolerance, healthy oil modifications and for transformation marker genes and selectable marker genes are used. DNA construct and transgenic plants containing the synthetic sequences are taught as are methods and compositions for using the plants in agriculture.

2. 发明专利

IL250915D0 Methods and compositions for recombination a gene-deficient strains of agrobacterium tumefaciens 未知
公开(公告)号：IL250915D0
公开(公告)日：2017-04-30
申请号：IL25091517
申请日：2017-03-02
申请人： DOW AGROSCIENCES LLC , DONALD J MERLO , NAGESH SARDESAI , SANDEEP KUMAR , MANJU GUPTA , SARA BENNETT
发明人： DONALD J MERLO , NAGESH SARDESAI , SANDEEP KUMAR , MANJU GUPTA , SARA BENNETT
IPC分类号： C12N20060101
摘要： The present disclosure provides novel compositions and methods for the production and use of Agrobacterium tumefaciens strains (for example LBA4404) that are deficient in RecA activity relative to the parent strain. Combinations with other gene-deficient-strains of Agrobacterium tumefaciens are also disclosed. Specifically, two exemplary s recA minus strains, UIA777 where chloramphenicol resistant gene disrupting the recA gene and UIA770 where kanamycin resistant gene disrupting the recA gene are provided.

3. 发明授权

US09617551B2 Method of increasing plant transformation frequency using modified strains of Agrobacteria 有权
公开(公告)号：US09617551B2
公开(公告)日：2017-04-11
申请号：US13812469
申请日：2011-07-29
申请人： Donald J. Merlo , Sean M. Russell , Diane M. Retallack , Aaron T. Woosley , Thomas Meade , Kenneth Narva
发明人： Donald J. Merlo , Sean M. Russell , Diane M. Retallack , Aaron T. Woosley , Thomas Meade , Kenneth Narva
IPC分类号： C12N15/82 , C12N15/74
CPC分类号： C12N15/8205 , C07K14/195 , C07K14/325 , C12N15/74 , C12N15/743 , C12N15/8274 , C12N15/8286 , Y02A40/162
摘要： Agrobacterium strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these Agrobacterium strains are provided. Additionally, Agrobacterium strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included are Agrobacterium strains that harbor transformation-enhancing genes integrated into the Agrobacterium chromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of the Agrobacterium cells, and uses for these Agrobacterium strains. Plants made using these Agrobacterium strains are also described.

4. 发明授权

US09434773B2 Plants having stress related traits and methods for making the same 有权
标题翻译：具有压力相关性状的植物及其制作方法
公开(公告)号：US09434773B2
公开(公告)日：2016-09-06
申请号：US13617207
申请日：2012-09-14
申请人： Avutu S. Reddy , Sun Yuejin , Donald J. Merlo , Weiting W. Ni , Dayakar Pareddy , Zhifang Gao , Gregory A. Bradfisch
发明人： Avutu S. Reddy , Sun Yuejin , Donald J. Merlo , Weiting W. Ni , Dayakar Pareddy , Zhifang Gao , Gregory A. Bradfisch
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82 , C12N9/00 , C07K14/415
CPC分类号： C07K14/415 , C12N9/93 , C12N15/8273 , C12Y603/02019
摘要： This invention relates generally to a plant cell with increased yield, preferably under condition of transient and repetitive abiotic stress as compared to a corresponding non-transformed wild type plant cell by increasing or generating one or more activities of RING proteins in plants.
摘要翻译：本发明一般涉及通过增加或产生植物中RING蛋白的一种或多种活性而与相应的非转化野生型植物细胞相比，优选在瞬时和重复的非生物胁迫条件下具有增加的产量的植物细胞。

5. 发明授权

US09427003B2 Synthetic genes 有权
标题翻译：合成基因
公开(公告)号：US09427003B2
公开(公告)日：2016-08-30
申请号：US13447836
申请日：2012-04-16
申请人： Ignacio M. Larrinua , Donald J. Merlo , Avutu S. Reddy , Arvind Kumar ThirumalaiswamySekhar , Aaron T. Woosley
发明人： Ignacio M. Larrinua , Donald J. Merlo , Avutu S. Reddy , Arvind Kumar ThirumalaiswamySekhar , Aaron T. Woosley
IPC分类号： C12N15/11 , C12N15/63 , A01H5/00 , A23L1/10 , A23D9/00 , A23L1/20 , A23L1/214 , C07K14/325 , C07K14/435 , C11B1/00 , C12N9/08 , C12N9/02 , C12N15/82
CPC分类号： C12N15/8286 , A23D9/00 , A23L7/10 , A23L7/198 , A23L11/00 , A23L11/05 , A23L19/10 , C07K14/195 , C07K14/325 , C07K14/37 , C07K14/38 , C07K14/415 , C07K14/43595 , C11B1/00 , C12N9/0065 , C12N9/0069 , C12N9/0083 , C12Y111/01015 , C12Y113/00 , Y02A40/162
摘要： The invention provides synthetic nucleic acid sequences encoding proteins of interest that are particularly adapted to express well in plants. The claimed synthetic sequences utilize plant-optimized codons roughly in the same frequency at which they are utilized, on average, in genes naturally occurring in the plant species. The invention further includes synthetic DNA sequence for herbicide tolerance, water and/or heat stress tolerance, healthy oil modifications and for transformation marker genes and selectable marker genes are used. DNA construct and transgenic plants containing the synthetic sequences are taught as are methods and compositions for using the plants in agriculture.
摘要翻译：本发明提供编码感兴趣的蛋白质的合成核酸序列，其特别适于在植物中表达良好。所要求的合成序列利用植物优化的密码子，其平均来说在天然存在于植物物种中的基因中使用大致相同的频率。本发明还包括用于除草剂耐受性，水和/或热胁迫耐受性，健康油改性和用于转化标记基因和选择标记基因的合成DNA序列。教导含有合成序列的DNA构建体和转基因植物，以及在农业中使用植物的方法和组合物。

6. 发明申请

US20120266335A1 SYNTHETIC GENES 有权
标题翻译：合成基因
公开(公告)号：US20120266335A1
公开(公告)日：2012-10-18
申请号：US13447836
申请日：2012-04-16
申请人： IGNACIO M. LARRINUA , DONALD J. MERLO , AVUTU S. REDDY , ARVIND KUMAR THIRUMALAISWAMYSEKHAR , AARON T. WOOSLEY
发明人： IGNACIO M. LARRINUA , DONALD J. MERLO , AVUTU S. REDDY , ARVIND KUMAR THIRUMALAISWAMYSEKHAR , AARON T. WOOSLEY
IPC分类号： C12N15/11 , C12N15/63 , A01H5/00 , C07K14/415 , C11B1/00 , C12N15/31 , A23P1/00 , A23L1/10 , A23L1/36 , B02C9/00 , C12N15/32 , C12N15/53 , A01H5/10
CPC分类号： C12N15/8286 , A23D9/00 , A23L7/10 , A23L7/198 , A23L11/00 , A23L11/05 , A23L19/10 , C07K14/195 , C07K14/325 , C07K14/37 , C07K14/38 , C07K14/415 , C07K14/43595 , C11B1/00 , C12N9/0065 , C12N9/0069 , C12N9/0083 , C12Y111/01015 , C12Y113/00 , Y02A40/162
摘要： The invention provides synthetic nucleic acid sequences encoding proteins of interest that are particularly adapted to express well in plants. The claimed synthetic sequences utilize plant-optimized codons roughly in the same frequency at which they are utilized, on average, in genes naturally occurring in the plant species. The invention further includes synthetic DNA sequence for herbicide tolerance, water and/or heat stress tolerance, healthy oil modifications and for transformation marker genes and selectable marker genes are used. DNA construct and transgenic plants containing the synthetic sequences are taught as are methods and compositions for using the plants in agriculture.
摘要翻译：本发明提供编码感兴趣的蛋白质的合成核酸序列，其特别适于在植物中表达良好。所要求的合成序列利用植物优化的密码子，其平均来说在天然存在于植物物种中的基因中使用大致相同的频率。本发明还包括用于除草剂耐受性，水和/或热胁迫耐受性，健康油改性和用于转化标记基因和选择标记基因的合成DNA序列。教导含有合成序列的DNA构建体和转基因植物，以及在农业中使用植物的方法和组合物。

7. 发明授权

US08003777B2 Use of untranslated region of osmotin gene to enhance transgene expression in plants 有权
公开(公告)号：US08003777B2
公开(公告)日：2011-08-23
申请号：US12012813
申请日：2008-02-05
申请人： Donald J. Merlo , Dong Liu , Stephanie L. Burton , Todd P. Glancy
发明人： Donald J. Merlo , Dong Liu , Stephanie L. Burton , Todd P. Glancy
IPC分类号： C07H21/04 , C12N15/00 , C12N15/113 , C12N15/63
CPC分类号： C12N15/8216 , C07H21/04
摘要： The present invention provides methods, vectors and gene constructs for enhancing expression of a recombinant nucleic acid sequence in transgenic plants and plant tissues. According to the present invention, nucleic acid sequences are obtained and/or derived from the 5′ and 3′ untranslated regions of genes encoding osmotin proteins and engineered to flank respective portions of a selected coding region of a vector. The vector construct may be introduced into plants and/or plant tissues through conventional procedures, resulting in enhanced expression of the selected coding region. In a preferred embodiment, the selected coding region is a chimeric gene or gene fragment expressing one or more proteins known to impart a level of insecticidal activity to a transgenic plant and/or plant tissue.

8. 发明申请

US20090221501A1 MIXING AND MATCHING TC PROTEINS FOR PEST CONTROL 有权
标题翻译：混合和匹配TC蛋白进行病虫害防治
公开(公告)号：US20090221501A1
公开(公告)日：2009-09-03
申请号：US12371825
申请日：2009-02-16
申请人： Timothy D. Hey , Amanda D. Schleper , Scott A. Bevan , Scott B. Bintrim , Jon C. Mitchell , Ze Sheng Li , Weiting Ni , Baolong Zhu , Donald J. Merlo , Patricia C. Apel-Birkhold , Thomas Meade
发明人： Timothy D. Hey , Amanda D. Schleper , Scott A. Bevan , Scott B. Bintrim , Jon C. Mitchell , Ze Sheng Li , Weiting Ni , Baolong Zhu , Donald J. Merlo , Patricia C. Apel-Birkhold , Thomas Meade
IPC分类号： A61K38/16 , A01P7/04
CPC分类号： C07K14/195 , A01N37/46 , A01N63/02 , C07K14/24 , C12N15/8286 , Y02A40/162
摘要： The subject invention relates to the surprising discovery that toxin complex (TC) proteins, obtainable from Xenorhabdus, Photorhabdus, and Paenibacillus, can be used interchangeably with each other. In particularly preferred embodiments of the subject invention, the toxicity of a “stand-alone” TC protein (from Photorhabdus, Xenorhabdus, or Paenibacillus, for example) is enhanced by one or more TC protein “potentiators” derived from a source organism of a different genus from which the toxin was derived. As one skilled in the art will recognize with the benefit of this disclosure, this has broad implications and expands the range of utility that individual types of TC proteins will now be recognized to have. Among the most important advantages is that one skilled in the art will now be able to use a single set of potentiators to enhance the activity of a stand-alone Xenorhabdus protein toxin as well as a stand-alone Photorhabdus protein toxin. (As one skilled in the art knows, Xenorhabdus toxin proteins tend to be more desirable for controlling lepidopterans while Photorhabdus toxin proteins tend to be more desirable for controlling coleopterans.) This reduces the number of genes, and transformation events, needed to be expressed by a transgenic plant to achieve effective control of a wider spectrum of target pests. Certain preferred combinations of heterologous TC proteins are also disclosed herein. Other objects, advantages, and features of the subject invention will be apparent to one skilled in the art having the benefit of the subject disclosure.
摘要翻译：本发明涉及令人惊奇的发现，可以从Xenorhabdus，Photorhabdus和Paenibacillus获得的毒素复合物（TC）蛋白质彼此互换使用。在本发明的特别优选的实施方案中，“独立”TC蛋白（例如，来自Photorhabdus，Xenorhabdus或Paenibacillus的例如）的毒性由一种或多种衍生自以下的源生物体的TC蛋白“增强剂”增强来源于毒素的不同属。本领域技术人员将会认识到本公开的益处，这具有广泛的含义并扩大了现在将认识到各种类型的TC蛋白质具有的效用范围。最重要的优点之一是，本领域技术人员现在将能够使用单一增强剂来增强独立的Xenorhabdus蛋白毒素的活性以及独立的Photorhabdus蛋白毒素。（如本领域技术人员所知，Xenorhabdus毒素蛋白质倾向于更好地控制鳞翅目，而Photorhabdus毒素蛋白质倾向于更有利于控制鞘翅目）。这减少了需要表达的基因数量和转化事件一个转基因植物，以实现更广泛的目标害虫的有效控制。异源TC蛋白的某些优选组合也在本文中公开。受益于本发明的本发明的其它目的，优点和特征对本领域技术人员而言是显而易见的。

9. 发明授权

US06528484B1 Insecticidal protein toxins from Photorhabdus 失效
公开(公告)号：US06528484B1
公开(公告)日：2003-03-04
申请号：US08851567
申请日：1997-05-05
申请人： Jerald C. Ensign , David J. Bowen , James Petell , Raymond Fatig , Sue Schoonover , Richard H. ffrench-Constant , Thomas A. Rocheleau , Michael B. Blackburn , Timothy D. Hey , Donald J. Merlo , Gregory L. Orr , Jean L. Roberts , James A. Strickland , Lining Guo , Todd A. Ciche , Kitisri Sukhapinda
发明人： Jerald C. Ensign , David J. Bowen , James Petell , Raymond Fatig , Sue Schoonover , Richard H. ffrench-Constant , Thomas A. Rocheleau , Michael B. Blackburn , Timothy D. Hey , Donald J. Merlo , Gregory L. Orr , Jean L. Roberts , James A. Strickland , Lining Guo , Todd A. Ciche , Kitisri Sukhapinda
IPC分类号： A01N3718
CPC分类号： C07K14/24 , A01N63/02 , C07K14/195 , C12N9/52 , C12N15/8286 , Y02A40/162
摘要： Proteins from the genus Photorhabdus are toxic to insects upon exposure. Photorhabdus luminescens (formerly Xenorhabdus luminescens) have been found in mammalian clinical samples and as a bacterial symbiont of entomopathogenic nematodes of genus Heterorhabditis. These protein toxins can be applied to, or genetically engineered into, insect larvae food and plants for insect control.

10. 发明授权

US5736627A Virus resistant plants having coat protein 失效
标题翻译：具有外壳蛋白的抗病毒植物
公开(公告)号：US5736627A
公开(公告)日：1998-04-07
申请号：US174829
申请日：1988-03-29
申请人： L. Sue Loesch-Fries , Nancy P. Jarvis , Donald J. Merlo
发明人： L. Sue Loesch-Fries , Nancy P. Jarvis , Donald J. Merlo
IPC分类号： A01H1/00 , A01H5/00 , C07K14/08 , C12N1/20 , C12N5/00 , C12N5/04 , C12N5/10 , C12N15/00 , C12N15/09 , C12N15/40 , C12N15/82 , C12N15/84 , C12R1/01 , C12R1/91
CPC分类号： C07K14/005 , C12N15/8283 , C12N5/04 , C12N2770/14022
摘要： The making of plant cells which contain coat protein of a target plant virus is disclosed. Construction of coat protein genes and transformation of coat protein genes into plant cells is also taught. Such cells are relatively resistant to infection by the target virus when compared with cells not containing coat protein. Furthermore, methods and DNA molecules useful for producing plant cells containing coat protein are also disclosed.
摘要翻译：公开了含有靶植物病毒外壳蛋白的植物细胞的制备。还教导了外壳蛋白基因的构建和外壳蛋白基因转化到植物细胞中。与不含外壳蛋白的细胞相比，这样的细胞相对于目标病毒的感染相对抗性。此外，还公开了可用于产生含有外壳蛋白的植物细胞的方法和DNA分子。

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