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1. 发明申请

WO2006086377A2 METHOD OF DETECTION OF CLASSICAL SWINE FEVER 审中-公开
标题翻译：检测经典开关的方法
公开(公告)号：WO2006086377A2
公开(公告)日：2006-08-17
申请号：PCT/US2006/004271
申请日：2006-02-08
申请人： IDEXX LABORATORIES , HOFFMANN, Bernd , DEPNER, Klaus , BEER, Martin
发明人： HOFFMANN, Bernd , DEPNER, Klaus , BEER, Martin
IPC分类号： C12Q1/70 , C12Q1/68 , C07J17/00 , C07H21/04
CPC分类号： C12Q1/70
摘要： The invention relates to a multiplex real-time RT-PCR assay with a heterologous internal control system (i.e., EGFP-RNA) for the simple and fast diagnosis of classical swine fever virus (CSFV). Primers and FAM-labeled TaqMan probes, specific for CSFV were selected by analyzing the consensus sequence of the 5 '-non translated region of various CSFV strains. For determining the analytical sensitivity an in vitro transcript (T7-PC3Alf) of the 5' NTR was constructed and tested. Furthermore, a primer - probe system for the detection of the internal control sequence was established, and a multiplex assay using CSF-System 1 and the IC real-time PCR could be performed as a one-tube assay without loss of sensitivity or specificity.
摘要翻译：本发明涉及用于简单和快速诊断典型猪瘟病毒（CSFV）的异源内部对照系统（即EGFP-RNA）的多重实时RT-PCR测定。通过分析各种CSFV毒株5'非翻译区的共有序列，选择引物和FAM标记的TaqMan探针，对CSFV特异性。为了确定分析灵敏度，构建并测试了5'NTR的体外转录物（T7-PC3Alf）。此外，建立了用于检测内部控制序列的引物 - 探针系统，使用CSF-System 1和IC实时PCR的多重测定可以作为单管测定进行，而不损失敏感性或特异性。

2. 发明申请

WO2006086377A3 METHOD OF DETECTION OF CLASSICAL SWINE FEVER 审中-公开
标题翻译：检测经典开关的方法
公开(公告)号：WO2006086377A3
公开(公告)日：2009-05-22
申请号：PCT/US2006004271
申请日：2006-02-08
申请人： IDEXX LAB , HOFFMANN BERND , DEPNER KLAUS , BEER MARTIN
发明人： HOFFMANN BERND , DEPNER KLAUS , BEER MARTIN
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/70
摘要： The invention relates to a multiplex real-time RT-PCR assay with a heterologous internal control system (i.e., EGFP-RNA) for the simple and fast diagnosis of classical swine fever virus (CSFV). Primers and FAM-labeled TaqMan probes, specific for CSFV were selected by analyzing the consensus sequence of the 5 '-non translated region of various CSFV strains. For determining the analytical sensitivity an in vitro transcript (T7-PC3Alf) of the 5' NTR was constructed and tested. Furthermore, a primer - probe system for the detection of the internal control sequence was established, and a multiplex assay using CSF-System 1 and the IC real-time PCR could be performed as a one-tube assay without loss of sensitivity or specificity.
摘要翻译：本发明涉及用于简单和快速诊断典型猪瘟病毒（CSFV）的异源内部对照系统（即EGFP-RNA）的多重实时RT-PCR测定。通过分析各种CSFV毒株5'非翻译区的共有序列，选择引物和FAM标记的TaqMan探针，对CSFV特异性。为了确定分析灵敏度，构建并测试了5'NTR的体外转录物（T7-PC3Alf）。此外，建立了用于检测内部控制序列的引物 - 探针系统，使用CSF-System 1和IC实时PCR的多重测定可以作为单管测定进行，而不损失敏感性或特异性。

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