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1. 发明申请

US20150023979A1 Antibodies Against E7 Protein of Human Papilloma Virus (HPV) 审中-公开
标题翻译：针对人乳头状瘤病毒（HPV）的E7蛋白的抗体
公开(公告)号：US20150023979A1
公开(公告)日：2015-01-22
申请号：US14344525
申请日：2012-09-06
申请人： Christian Kuhne
发明人： Christian Kuhne
IPC分类号： C07K16/08 , C12N7/00 , C07K14/005
CPC分类号： C07K16/084 , C07K14/005 , C07K2317/33 , C07K2317/82 , C07K2317/92 , C12N7/00 , C12N2710/20034 , C12N2710/20051 , G01N33/56983 , G01N2333/025
摘要： The invention discloses a method for preparing Human Papilloma Virus protein E7 antigen (HPV E7 antigen) comprising the following steps: —providing a purified preparation of HPV protein E7; —phosphorylating the HPV protein E7 in the preparation; —purifying the phosphorylated E7 protein with an anion exchange chromatography, wherein the phosphorylated E7 protein is separated from the non-phosphorylated E7 protein by a step wherein the non-phosphorylated E7 protein stays bound to an anion exchanger during the anion exchange chromatography whereas the phosphorylated E7 protein is obtained in the eluate of the anion exchange chromatography, thereby—obtaining a purified preparation of a phosphorylated HPV protein E7 antigen. The invention further discloses antibodies specific to this antigen, a kit and method for using such antibodies in clinical diagnostics and methods for generation of such antibodies.
摘要翻译：本发明公开了一种制备人乳头状瘤病毒蛋白E7抗原（HPV E7抗原）的方法，包括以下步骤：提供HPV蛋白E7纯化制剂; 在制备中磷酸化HPV蛋白E7; 用阴离子交换层析纯化磷酸化的E7蛋白质，其中通过一个步骤将磷酸化的E7蛋白与非磷酸化的E7蛋白分离，其中非磷酸化的E7蛋白在阴离子交换层析期间与阴离子交换剂结合，而磷酸化的在阴离子交换层析的洗脱液中获得E7蛋白质，从而获得磷酸化的HPV蛋白E7抗原的纯化制剂。本发明还公开了对该抗原特异性的抗体，在临床诊断中使用这些抗体的试剂盒和方法以及用于产生这种抗体的方法。

2. 发明申请

US20110033384A1 CHIMERIC POLYPEPTIDES AND THEIR USE 审中-公开
标题翻译： CHIMERIC POLYPEPTIDES及其使用
公开(公告)号：US20110033384A1
公开(公告)日：2011-02-10
申请号：US12616859
申请日：2009-11-12
申请人： Christian KUHNE , Andras Simoncsits
发明人： Christian KUHNE , Andras Simoncsits
IPC分类号： A61K49/00 , A61K31/713 , A61K35/12 , A61K38/46 , A61P35/00
CPC分类号： C12N9/22 , A61K48/00 , C07K7/06 , C07K2319/33 , C12N15/62 , C12Y301/21004
摘要： The presented invention concerns chimeric molecules that contain a preferential polypeptidic region, consisting of a specific affinity for the binding to specific DNA sequences, of a preferential polypeptidic region consisting of a DNA modifying activity, and this chimeric molecule is capable to cross biological membranes due to the presence of a region that contains delivery activity. The invention contains further the isolated polynucleotides that code for the chimeric molecules of the invention if they are as such entirely or partially of polypeptide nature.In another embodiment, based on the activities of the polypeptides contained in the invention to interfere with key points of the cell-cycle regulation and the cellular checkpoints due to their introduction of DNA double strand breaks, the invention contains various procedures that are characterized by the use of said polypeptides of the invention for cells in vivo and provides an activity for the modification of specific sites of the DNA contained in a cell. The invention also contains procedures that use the chimeric molecules of the invention to screen for new delivery activities or combinations of delivery activities. The invention further provides for the therapeutic use of said compositions as anti-proliferative, anti-neoplastic, antibiotic, antiparasitic or antiviral agents.
摘要翻译：所提出的发明涉及嵌合分子，该嵌合分子含有由与特定DNA序列结合的特异性亲和力的优先多肽区域，其由DNA修饰活性组成的优先多肽区域，并且该嵌合分子能够跨越生物膜，由于存在包含递送活动的区域。本发明进一步包含编码本发明嵌合分子的分离的多核苷酸，如果它们完全或部分为多肽性质。在另一个实施方案中，基于本发明中包含的多肽干扰细胞周期调节的关键点和由于其引入DNA双链断裂引起的细胞检查点的活性，本发明包含各种程序，其特征在于在体内使用本发明的所述多肽用于细胞，并提供用于修饰细胞中包含的DNA的特定位点的活性。本发明还包括使用本发明的嵌合分子筛选新的递送活性或递送活性的组合的程序。本发明还提供了所述组合物作为抗增殖，抗肿瘤，抗生素，抗寄生虫或抗病毒剂的治疗用途。

3. 发明申请

US20060292629A1 Chimeric polypeptides and their use 审中-公开
标题翻译：嵌合多肽及其用途
公开(公告)号：US20060292629A1
公开(公告)日：2006-12-28
申请号：US10546661
申请日：2004-04-16
申请人： Christian Kuhne , Andras Simoncsits
发明人： Christian Kuhne , Andras Simoncsits
IPC分类号： G01N33/53 , A61K38/17 , C07K14/82 , C07K14/47 , C07H21/04 , C12P21/06
CPC分类号： C12N9/22 , A61K48/00 , C07K7/06 , C07K2319/33 , C12N15/62 , C12Y301/21004
摘要： The present invention concerns chimeric molecules that contain a preferential polypeptidic region, consisting of a specific affinity for the binding to specific DNA sequences, of a preferential polypeptidic region consisting of a DNA modifying activity, and this chimeric molecule is capable to cross biological membranes due to the presence of a region that contains delivery activity. The invention contains further the isolated polynucleotides that code for the chimeric molecules of the invention if they are as such entirely or partially of polypeptide nature. In another embodiment, based on the activities of the polypeptides contained in the invention to interfere with key points of the cell-cycle regulation and the cellular checkpoints due to their introduction of DNA double strand breaks, the invention contains various procedures that are characterized by the use of said polypeptides of the invention for cells in vivo and provides an activity for the modification of specific sites of the DNA contained in a cell. The invention also contains procedures that use the chimeric molecules of the invention to screen for new delivery activities or combinations of delivery activities. The invention further provides for the therapeutic use of said compositions as anti-prolierative, anti-neoplastic, antibiotic, antiparasitic or antiviral agents.
摘要翻译：本发明涉及包含优选多肽区域的嵌合分子，其由与特定DNA序列结合的特异性亲和力组成，由DNA修饰活性构成的优先多肽区域，并且该嵌合分子能够跨越生物膜由于存在包含递送活动的区域。本发明进一步包含编码本发明嵌合分子的分离的多核苷酸，如果它们完全或部分为多肽性质。在另一个实施方案中，基于本发明中包含的多肽干扰细胞周期调节的关键点和由于其引入DNA双链断裂引起的细胞检查点的活性，本发明包含各种方法，其特征在于在体内使用本发明的所述多肽用于细胞，并提供用于修饰细胞中包含的DNA的特定位点的活性。本发明还包括使用本发明的嵌合分子筛选新的递送活性或递送活性的组合的程序。本发明进一步提供了所述组合物作为抗雌激素，抗肿瘤，抗生素，抗寄生虫或抗病毒药物的治疗用途。

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