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1. 发明申请

WO2004063338A3 METHOS FOR ANALYZING GLOBAL REGULATION OF CODING AND NON-CODING RNA TRANSCRIPTS INVOLVING LOW MOLECULAR WEIGHT RNAS 审中-公开
公开(公告)号：WO2004063338A3
公开(公告)日：2004-07-29
申请号：PCT/US2004/000379
申请日：2004-01-08
申请人： AFFYMETRIX, INC. , KAPRANOV, Philipp, V. , KAMPA, Dione , GINGERAS, Thomas, R. , BEKIRANOV, Stefan , CAWLEY, Simon , COLE, Kyle, B.
发明人： KAPRANOV, Philipp, V. , KAMPA, Dione , GINGERAS, Thomas, R. , BEKIRANOV, Stefan , CAWLEY, Simon , COLE, Kyle, B.
IPC分类号： C12Q1/68
摘要： In some embodiments of the invention, methods are provided to interrogate the transcriptional activity relating to RNAs of small molecular weight. The methods employ hybridization of a large number of oligonucleotide probes with nucleic acid derived from RNAs of small molecular weight.

2. 发明申请

WO2004007751A3 NUCLEIC ACID LABELING METHODS 审中-公开
公开(公告)号：WO2004007751A3
公开(公告)日：2004-01-22
申请号：PCT/US2003/022035
申请日：2003-07-14
申请人： AFFYMETRIX, INC. , COLE, Kyle, B. , TRUONG, Vivi , MCGALL, Glenn, H. , BARONE, Anthony, D.
发明人： COLE, Kyle, B. , TRUONG, Vivi , MCGALL, Glenn, H. , BARONE, Anthony, D.
IPC分类号： C12Q1/68
摘要： In one aspect of the invention, a method is provided for end-labeling RNA (total RNA, mRNA, cRNA or fragmented RNA). In one embodiment, T4 RNA ligase is used to attach a 3'-biotinylated AMP or CMP donor to an RNA acceptor molecule. In another embodiment, a pyrophosphate molecule 3'-AppN-3'-linker-biotin is used as donor molecule. In another aspect of the invention, a method is provided for analyzing a nucleic acid population on a nucleic acid microarray comprising providing a nucleic acid population or converting the nucleic acid population into nucleic acid fragments; ligating the nucleic acid population or fragments to a labeled nucleic acid molecule to form labeled nucleic acid population or fragments using a ligase; hybridizing the labeled nucleic acid population or fragments to an array of nucleic acid probes, and determining hybridization signals of the probes as an indication of levels of the nucleic acids in the nucleic acid population.

3. 发明申请

WO2004007751A2 NUCLEIC ACID LABELING METHODS 审中-公开
标题翻译：核酸标记方法
公开(公告)号：WO2004007751A2
公开(公告)日：2004-01-22
申请号：PCT/US0322035
申请日：2003-07-14
申请人： AFFYMETRIX INC , COLE KYLE B , TRUONG VIVI , MCGALL GLENN H , BARONE ANTHONY D
发明人： COLE KYLE B , TRUONG VIVI , MCGALL GLENN H , BARONE ANTHONY D
IPC分类号： C07H19/10 , C07H19/20 , C07H21/00 , C12Q1/68 , C12Q
CPC分类号： C07H21/00 , C07H19/10 , C07H19/20 , C12Q1/6816 , C12Q2565/501 , C12Q2563/107 , C12Q2521/501
摘要： In one aspect of the invention, a method is provided for end-labeling RNA (total RNA, mRNA, cRNA or fragmented RNA). In one embodiment, T4 RNA ligase is used to attach a 3'-biotinylated AMP or CMP donor to an RNA acceptor molecule. In another embodiment, a pyrophosphate molecule 3'-AppN-3'-linker-biotin is used as donor molecule. In another aspect of the invention, a method is provided for analyzing a nucleic acid population on a nucleic acid microarray comprising providing a nucleic acid population or converting the nucleic acid population into nucleic acid fragments; ligating the nucleic acid population or fragments to a labeled nucleic acid molecule to form labeled nucleic acid population or fragments using a ligase; hybridizing the labeled nucleic acid population or fragments to an array of nucleic acid probes, and determining hybridization signals of the probes as an indication of levels of the nucleic acids in the nucleic acid population.
摘要翻译：在本发明的一个方面，提供了用于末端标记RNA（总RNA，mRNA，cRNA或片段化的RNA）的方法。在一个实施方案中，T4 RNA连接酶用于将3'-生物素化的AMP或CMP供体附着至RNA受体分子。在另一个实施方案中，使用焦磷酸分子3'-AppN-3'-连接体 - 生物素作为供体分子。在本发明的另一方面，提供了用于分析核酸微阵列上的核酸群体的方法，其包括提供核酸群体或将核酸群体转换成核酸片段; 使用连接酶将核酸群或片段连接至标记的核酸分子以形成标记的核酸群或片段; 将标记的核酸群体或片段与核酸探针阵列杂交，并确定探针的杂交信号，作为核酸群体中核酸水平的指示。

4. 发明申请

WO2004063338A2 METHOS FOR ANALYZING GLOBAL REGULATION OF CODING AND NON-CODING RNA TRANSCRIPTS INVOLVING LOW MOLECULAR WEIGHT RNAS 审中-公开
标题翻译：用于分析涉及低分子量RNA的编码和非编码RNA转录物的全球调控的甲基化
公开(公告)号：WO2004063338A2
公开(公告)日：2004-07-29
申请号：PCT/US2004000379
申请日：2004-01-08
申请人： AFFYMETRIX INC , KAPRANOV PHILIPP V , KAMPA DIONE , GINGERAS THOMAS R , BEKIRANOV STEFAN , CAWLEY SIMON , COLE KYLE B
发明人： KAPRANOV PHILIPP V , KAMPA DIONE , GINGERAS THOMAS R , BEKIRANOV STEFAN , CAWLEY SIMON , COLE KYLE B
IPC分类号： C12N20060101 , C12Q1/68 , C12N
CPC分类号： C12Q1/6837 , C12Q1/6876 , C12Q2600/158 , C12Q2600/178 , C12Q2565/507
摘要： In some embodiments of the invention, methods are provided to interrogate the transcriptional activity relating to RNAs of small molecular weight. The methods employ hybridization of a large number of oligonucleotide probes with nucleic acid derived from RNAs of small molecular weight.
摘要翻译：在本发明的一些实施方案中，提供了用于询问与小分子量的RNA相关的转录活性的方法。该方法使用大量寡核苷酸探针与衍生自小分子量RNA的核酸杂交。

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