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1. 发明专利

CA2878263A1 NOVEL INSECTICIDAL PROTEINS AND METHODS FOR THEIR USE 未知
公开(公告)号：CA2878263A1
公开(公告)日：2014-01-09
申请号：CA2878263
申请日：2013-06-26
申请人： PIONEER HI BRED INT , CERF DAVID C , ENGLISH JAMES J , HENDRICK CAROL A , LIU LU , ORAL JARRED K , PATTEN PHILLIP A , ROSEN BARBARA A , SCHELLENBERGER UTE , UDRANSZKY INGRID A , WEI JUN-ZHI , ZHU GENHAI
发明人： CERF DAVID C , ENGLISH JAMES J , HENDRICK CAROL A , LIU LU , ORAL JARRED K , PATTEN PHILLIP A , ROSEN BARBARA A , SCHELLENBERGER UTE , UDRANSZKY INGRID A , WEI JUN-ZHI , ZHU GENHAI
IPC分类号： C07K14/21 , A01N43/50 , A61K45/06 , C07K16/12 , G01N33/68
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest, as probes for the isolation of other homologous (or partially homologous) genes. The insecticidal proteins find use in controlling, inhibiting growth or killing lepidopteran, coleopteran, dipteran, fungal, hemipteran, and nematode pest populations and for producing compositions with insecticidal activity.

2. 发明申请

WO2011082107A1 DIRECT AND CONTINUOUS ROOT ALONE OR ROOT/SHOOT PRODUCTION FROM TRANSGENIC EVENTS DERIVED FROM GREEN REGENERATIVE TISSUES AND ITS APPLICATIONS 审中-公开
标题翻译：从绿色再生组织衍生的转基因事件及其应用中直接和连续的根源或根/生产
公开(公告)号：WO2011082107A1
公开(公告)日：2011-07-07
申请号：PCT/US2010/062018
申请日：2010-12-23
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , CHO, Myeong-je , CERF, David, C. , XU, Deping , ZHAO, Zuo-yu
发明人： CHO, Myeong-je , CERF, David, C. , XU, Deping , ZHAO, Zuo-yu
IPC分类号： C12N15/82
CPC分类号： C12N15/821 , A01G22/00 , C12N15/8201 , C12N15/8227 , C12N15/8286 , G01N33/5097 , G01N2333/415 , G01N2333/43552 , Y02A40/162
摘要： The present invention provides assays and methods for efficiently testing a polynucleotide of interest for a phenotype in a root. In some embodiments, the assays and methods include regenerating green tissue that is transgenic for at least one polynucleotide of interest into one or more transgenic plantlets that have at least one transgenic root. Further provided are methods of making a root assay by contacting green tissue with a first rooting medium to produce a plantlet and a plurality of roots. Additionally provided are methods of assaying for insecticidal activity on a live root. Accordingly provided herein is a substantially contamination- free, root bioassay. Further provided are methods of identifying a promoter having activity in a root.
摘要翻译：本发明提供了用于有效测试根中表型的目的多核苷酸的测定和方法。在一些实施方案中，测定和方法包括将至少一种感兴趣的多核苷酸转基因的绿色组织再生成具有至少一个转基因根的一个或多个转基因植物。还提供了通过使绿色组织与第一生根培养基接触以产生小植物和多个根来进行根分析的方法。另外提供了测定活根的杀虫活性的方法。因此本文提供了基本上无污染的根生物测定。还提供了鉴定在根中具有活性的启动子的方法。

3. 发明申请

WO2014008054A2 NOVEL INSECTICIDAL PROTEINS AND METHODS FOR THEIR USE 审中-公开
标题翻译：新型杀虫蛋白及其使用方法
公开(公告)号：WO2014008054A2
公开(公告)日：2014-01-09
申请号：PCT/US2013/047760
申请日：2013-06-26
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , CERF, David C. , ENGLISH, James J. , HENDRICK, Carol A. , LIU, Lu , ORAL, Jarred K. , PATTEN, Phillip A. , ROSEN, Barbara A. , SCHELLENBERGER, Ute , UDRANSZKY, Ingrid A. , WEI, Jun-Zhi , ZHU, Genhai
发明人： CERF, David C. , ENGLISH, James J. , HENDRICK, Carol A. , LIU, Lu , ORAL, Jarred K. , PATTEN, Phillip A. , ROSEN, Barbara A. , SCHELLENBERGER, Ute , UDRANSZKY, Ingrid A. , WEI, Jun-Zhi , ZHU, Genhai
IPC分类号： C07K14/21 , A01N43/50 , A61K45/06 , C07K16/12 , G01N33/68
CPC分类号： C07K14/21 , A01N43/50 , A61K45/06 , C07K16/1214 , C07K2319/24 , G01N33/68
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest, as probes for the isolation of other homologous (or partially homologous) genes. The insecticidal proteins find use in controlling, inhibiting growth or killing lepidopteran, coleopteran, dipteran, fungal, hemipteran, and nematode pest populations and for producing compositions with insecticidal activity.
摘要翻译：提供了控制害虫的组合物和方法。该方法涉及用编码杀虫蛋白的核酸序列转化生物体。特别地，核酸序列可用于制备具有杀虫活性的植物和微生物。因此，提供了转化的细菌，植物，植物细胞，植物组织和种子。组合物是杀虫的核酸和细菌物种的蛋白质。这些序列用于构建表达载体，用于随后转化为感兴趣的生物体，作为分离其他同源（或部分同源的）基因的探针。杀虫蛋白用于控制，抑制生长或杀死鳞翅目，鞘翅目，dipteran，真菌，半翅目和线虫害虫种群，并用于生产具有杀虫活性的组合物。

4. 发明申请

WO2011153418A3 COMPOSITIONS AND METHODS FOR INSECTICIDAL CONTROL OF STINKBUGS 审中-公开
标题翻译： STINKBUGS的杀虫剂控制组合物和方法
公开(公告)号：WO2011153418A3
公开(公告)日：2012-05-31
申请号：PCT/US2011039042
申请日：2011-06-03
申请人： BROGLIE KAREN E , CERF DAVID C , HERRMANN RAFAEL , LU ALBERT L , MCGONIGLE BRIAN , PRESNAIL JAMES K
发明人： BROGLIE KAREN E , CERF DAVID C , HERRMANN RAFAEL , LU ALBERT L , MCGONIGLE BRIAN , PRESNAIL JAMES K
IPC分类号： A01H5/00 , C12N15/31 , C12N15/82
CPC分类号： C12N15/8286 , A01N57/16 , A01N65/00 , A01N65/44 , C12N15/113 , C12N15/8218 , C12N2310/14 , C12N2310/531 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Pentatomidae plant pest or a N. viridula, Acrosternum hilare, Piezodorus guildini, and/or Halymorpha halys plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-292 or 302-304 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Pentatomidae. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉积元件的方法和组合物，当被害虫摄入时，例如五子科植物害虫或N. viridula，Acrosternum hilare，Piezodorus guildini和/或Halymorpha halys植物害虫，降低靶的表达序列在害虫中。在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。本发明提供了SEQ ID NO：1-292或302-304中任一项所述的各种靶多核苷酸或其活性变体和片段，其中目标害虫中一个或多个序列的表达降低控制害虫（即具有杀虫活性）。进一步提供的是沉积元件，当被害虫摄入时，降低靶多肽的水平，从而控制害虫。在具体实施方案中，害虫是五角锥科。还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。

5. 发明申请

WO2011153418A2 COMPOSITIONS AND METHODS FOR INSECTICIDAL CONTROL OF STINKBUGS 审中-公开
标题翻译： STINKBUGS的杀虫剂控制组合物和方法
公开(公告)号：WO2011153418A2
公开(公告)日：2011-12-08
申请号：PCT/US2011/039042
申请日：2011-06-03
申请人： BROGLIE, Karen, E. , CERF, David, C. , HERRMANN, Rafael , LU, Albert, L. , McGONIGLE, Brian , PRESNAIL, James, K.
发明人： BROGLIE, Karen, E. , CERF, David, C. , HERRMANN, Rafael , LU, Albert, L. , McGONIGLE, Brian , PRESNAIL, James, K.
IPC分类号： A01H5/00 , C12N15/31 , C12N15/82
CPC分类号： C12N15/8286 , A01N57/16 , A01N65/00 , A01N65/44 , C12N15/113 , C12N15/8218 , C12N2310/14 , C12N2310/531 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Pentatomidae plant pest or a N. viridula, Acrosternum hilare, Piezodorus guildini , and/or Halymorpha halys plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-292 or 302-304 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Pentatomidae . Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉积元件的方法和组合物，当被害虫摄入时，例如五子科植物害虫或N. viridula，Acrosternum hilare，Piezodorus guildini和/或Halymorpha halys植物害虫，降低靶的表达序列在害虫中。在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。本发明提供了SEQ ID NO：1-292或302-304中任一项所述的各种靶多核苷酸或其活性变体和片段，其中目标害虫中一个或多个序列的表达降低控制害虫（即具有杀虫活性）。进一步提供的是沉积元件，当被害虫摄入时，降低靶多肽的水平，从而控制害虫。在具体实施方案中，害虫是五角锥科。还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。

6. 发明申请

WO2014008054A3 NOVEL INSECTICIDAL PROTEINS AND METHODS FOR THEIR USE 审中-公开
标题翻译：新型杀虫蛋白及其使用方法
公开(公告)号：WO2014008054A3
公开(公告)日：2015-08-20
申请号：PCT/US2013047760
申请日：2013-06-26
申请人： PIONEER HI BRED INT , CERF DAVID C , ENGLISH JAMES J , HENDRICK CAROL A , LIU LU , ORAL JARRED K , PATTEN PHILLIP A , ROSEN BARBARA A , SCHELLENBERGER UTE , UDRANSZKY INGRID A , WEI JUN-ZHI , ZHU GENHAI
发明人： CERF DAVID C , ENGLISH JAMES J , HENDRICK CAROL A , LIU LU , ORAL JARRED K , PATTEN PHILLIP A , ROSEN BARBARA A , SCHELLENBERGER UTE , UDRANSZKY INGRID A , WEI JUN-ZHI , ZHU GENHAI
IPC分类号： C07K14/21
CPC分类号： C07K14/21 , A01N43/50 , A61K45/06 , C07K16/1214 , C07K2319/24 , G01N33/68
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest, as probes for the isolation of other homologous (or partially homologous) genes. The insecticidal proteins find use in controlling, inhibiting growth or killing lepidopteran, coleopteran, dipteran, fungal, hemipteran, and nematode pest populations and for producing compositions with insecticidal activity.
摘要翻译：提供了控制害虫的组成和方法。该方法涉及用编码杀虫蛋白的核酸序列转化生物体。特别地，核酸序列可用于制备具有杀虫活性的植物和微生物。因此，提供了转化的细菌，植物，植物细胞，植物组织和种子。组合物是杀虫的核酸和细菌物种的蛋白质。这些序列用于构建表达载体，用于随后转化为感兴趣的生物体，作为用于分离其他同源（或部分同源的）基因的探针。杀虫蛋白用于控制，抑制生长或杀死鳞翅目，鞘翅目，dipteran，真菌，半翅目和线虫害虫种群，并用于生产具有杀虫活性的组合物。

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