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1. 发明申请

WO2011025860A1 COMPOSITIONS AND METHODS TO CONTROL INSECT PESTS 审中-公开
标题翻译：组合物和方法来控制污染物
公开(公告)号：WO2011025860A1
公开(公告)日：2011-03-03
申请号：PCT/US2010/046762
申请日：2010-08-26
申请人： E. I. DU PONT DE NEMOURS AND COMPANY , BROGLIE, Karen, E. , KRISS, Kevin , LU, Albert, Laurence , MUTHALAGI, Mani , PRESNAIL, James, K.
发明人： BROGLIE, Karen, E. , KRISS, Kevin , LU, Albert, Laurence , MUTHALAGI, Mani , PRESNAIL, James, K.
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： A01N57/16 , A01N63/00 , A01N65/20 , A01N65/44 , C12N15/113 , C12N15/8218 , C12N15/8286 , C12N2310/11 , C12N2310/531 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or a Diabrotica plant pest decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-236 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is D. virgifera virgifera, D. barber i, D. speciosa, or D. undecimpimctata howardi, Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉积元件的方法和组合物，当由有害生物摄入时，例如鞘翅目植物病虫害病毒（Diabrotica plant pest），害虫减少害虫中靶序列的表达。在具体实施方案中，靶序列的表达降低控制害虫，由此方法和组合物能够限制对植物的损害。本发明提供了SEQ ID NO：1-236中任一项所述的各种目标多核苷酸或其活性变体和片段，其中目标害虫中一个或多个序列的表达降低控制害虫（即具有杀虫活性活动）。进一步提供的是沉积元件，当受害虫摄入时，降低靶多肽的水平，从而控制害虫。在具体实施方案中，有害生物是D. virgifera virgifera，D.berber i，D. speciosa或D. undecimpimctata howardi，植物，植物部分，细菌和其它宿主细胞，其包含沉默元件或其活性变体或片段还提供了发明。

2. 发明申请

WO0006755A3 MODIFICATION OF STARCH BIOSYNTHETIC ENZYME GENE EXPRESSION TO PRODUCE STARCHES IN GRAIN CROPS 审中-公开
标题翻译：淀粉合成酶基因表达载体在淀粉生产中的应用
公开(公告)号：WO0006755A3
公开(公告)日：2000-09-08
申请号：PCT/US9916296
申请日：1999-07-26
申请人： DU PONT , BROGLIE KAREN E , LIGHTNER JONATHAN EDWARD
发明人： BROGLIE KAREN E , LIGHTNER JONATHAN EDWARD
IPC分类号： A01H5/00 , A01H1/00 , C08B30/00 , C08B33/00 , C12N9/10 , C12N15/09 , C12N15/113 , C12N15/29 , C12N15/54 , C12N15/82 , C12N15/11
CPC分类号： C12N15/1137 , C08B30/00 , C12N9/1051 , C12N15/8245
摘要： The instant invention discloses utilization of a cDNA clone to construct sense and antisense genes for inhibition of starch synthase enzymatic activity in corn. More specifically, this invention concerns a method of controlling the starch fine structure of starch derived from the grain of cereal crops comprising: (1) preparing a chimeric gene comprising a nucleic acid fragment encoding a starch synthase structural gene or a fragment thereof, operably linked in either sense or antisense orientation on the upstream side to a nucleic acid fragment encoding a promoter that directs gene expression in corn endosperm tissue, and operably linked on the downstream side to a nucleic acid fragment encoding a suitable regulatory sequence for transcriptional termination; and (2) transforming cereal crops with said chimeric gene, wherein expression of said chimeric gene results in alteration of the fine structure of starch derived from the grain of said transformed cereal crops compared to the fine structure of starch derived from cereal crops not possessing said chimeric gene.
摘要翻译：本发明公开了利用cDNA克隆构建用于抑制玉米中淀粉合酶酶活性的有义和反义基因。更具体地说，本发明涉及一种控制来源于谷类作物谷物的淀粉的淀粉细结构的方法，包括：（1）制备包含编码淀粉合酶结构基因或其片段的核酸片段的嵌合基因，所述核酸片段可操作地连接在上游侧以有义或反义方向与编码指导玉米胚乳组织中基因表达的启动子的核酸片段连接，并且在下游侧与编码用于转录终止的合适调控序列的核酸片段可操作连接; 和（2）用所述嵌合基因转化谷类作物，其中所述嵌合基因的表达导致来自所述转化的谷类作物籽粒的淀粉的细微结构与来自不具有所述谷类作物的谷类作物的淀粉的精细结构相比改变嵌合基因。

3. 发明申请

WO2011153418A3 COMPOSITIONS AND METHODS FOR INSECTICIDAL CONTROL OF STINKBUGS 审中-公开
标题翻译： STINKBUGS的杀虫剂控制组合物和方法
公开(公告)号：WO2011153418A3
公开(公告)日：2012-05-31
申请号：PCT/US2011039042
申请日：2011-06-03
申请人： BROGLIE KAREN E , CERF DAVID C , HERRMANN RAFAEL , LU ALBERT L , MCGONIGLE BRIAN , PRESNAIL JAMES K
发明人： BROGLIE KAREN E , CERF DAVID C , HERRMANN RAFAEL , LU ALBERT L , MCGONIGLE BRIAN , PRESNAIL JAMES K
IPC分类号： A01H5/00 , C12N15/31 , C12N15/82
CPC分类号： C12N15/8286 , A01N57/16 , A01N65/00 , A01N65/44 , C12N15/113 , C12N15/8218 , C12N2310/14 , C12N2310/531 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Pentatomidae plant pest or a N. viridula, Acrosternum hilare, Piezodorus guildini, and/or Halymorpha halys plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-292 or 302-304 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Pentatomidae. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉积元件的方法和组合物，当被害虫摄入时，例如五子科植物害虫或N. viridula，Acrosternum hilare，Piezodorus guildini和/或Halymorpha halys植物害虫，降低靶的表达序列在害虫中。在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。本发明提供了SEQ ID NO：1-292或302-304中任一项所述的各种靶多核苷酸或其活性变体和片段，其中目标害虫中一个或多个序列的表达降低控制害虫（即具有杀虫活性）。进一步提供的是沉积元件，当被害虫摄入时，降低靶多肽的水平，从而控制害虫。在具体实施方案中，害虫是五角锥科。还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。

4. 发明申请

WO2011153418A2 COMPOSITIONS AND METHODS FOR INSECTICIDAL CONTROL OF STINKBUGS 审中-公开
标题翻译： STINKBUGS的杀虫剂控制组合物和方法
公开(公告)号：WO2011153418A2
公开(公告)日：2011-12-08
申请号：PCT/US2011/039042
申请日：2011-06-03
申请人： BROGLIE, Karen, E. , CERF, David, C. , HERRMANN, Rafael , LU, Albert, L. , McGONIGLE, Brian , PRESNAIL, James, K.
发明人： BROGLIE, Karen, E. , CERF, David, C. , HERRMANN, Rafael , LU, Albert, L. , McGONIGLE, Brian , PRESNAIL, James, K.
IPC分类号： A01H5/00 , C12N15/31 , C12N15/82
CPC分类号： C12N15/8286 , A01N57/16 , A01N65/00 , A01N65/44 , C12N15/113 , C12N15/8218 , C12N2310/14 , C12N2310/531 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Pentatomidae plant pest or a N. viridula, Acrosternum hilare, Piezodorus guildini , and/or Halymorpha halys plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-292 or 302-304 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Pentatomidae . Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉积元件的方法和组合物，当被害虫摄入时，例如五子科植物害虫或N. viridula，Acrosternum hilare，Piezodorus guildini和/或Halymorpha halys植物害虫，降低靶的表达序列在害虫中。在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。本发明提供了SEQ ID NO：1-292或302-304中任一项所述的各种靶多核苷酸或其活性变体和片段，其中目标害虫中一个或多个序列的表达降低控制害虫（即具有杀虫活性）。进一步提供的是沉积元件，当被害虫摄入时，降低靶多肽的水平，从而控制害虫。在具体实施方案中，害虫是五角锥科。还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。

5. 发明申请

WO0114540A3 STARCH SYNTHASE ISOFORM V 审中-公开
公开(公告)号：WO0114540A3
公开(公告)日：2001-12-06
申请号：PCT/US0023231
申请日：2000-08-24
申请人： DU PONT , ALLEN STEPHEN M , BROGLIE KAREN E , THORPE CATHERINE J
发明人： ALLEN STEPHEN M , BROGLIE KAREN E , THORPE CATHERINE J
IPC分类号： C12N9/10 , C12N15/82 , C12N1/15 , C12N1/21 , C12N5/10 , C12N15/54
CPC分类号： C12N9/1051 , C12N15/8245
摘要： This invention relates to an isolated nucleic acid fragment encoding a starch synthase isoform V. The invention also relates to the construction of a chimeric gene encoding all or a portion of the starch synthase isoform V, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the starch synthase isoform V in a transformed host cell.
摘要翻译：本发明涉及编码淀粉合酶同种型V的分离的核酸片段。本发明还涉及在正义或反义方向上编码全部或部分淀粉合成酶同种型V的嵌合基因的构建，其中嵌合基因导致转化宿主细胞中淀粉合成酶异构体V水平的变化。

6. 发明申请

WO2006107931A3 POLYNUCLEOTIDES AND METHODS FOR MAKING PLANTS RESISTANT TO FUNGAL PATHOGENS 审中-公开
标题翻译：用于制造抗真菌病原体的植物的多核苷酸和方法
公开(公告)号：WO2006107931A3
公开(公告)日：2006-12-07
申请号：PCT/US2006012443
申请日：2006-04-04
申请人： DU PONT , PIONEER HI BRED INT , UNIV DELAWARE , BROGLIE KAREN E , BUTLER KARLENE H , BUTRUILLE MARYMAR G , DA SILVA CONCEICAO ALEXANDRE , FREY TRAVIS J , HAWK JAMES A , JAQUETH JENNIFER S , JONES ELIZABETH S , MULTANI DILBAG S , WOLTERS PETRA J
发明人： BROGLIE KAREN E , BUTLER KARLENE H , BUTRUILLE MARYMAR G , DA SILVA CONCEICAO ALEXANDRE , FREY TRAVIS J , HAWK JAMES A , JAQUETH JENNIFER S , JONES ELIZABETH S , MULTANI DILBAG S , WOLTERS PETRA J
IPC分类号： C12N15/82 , A01H5/00 , C12N15/29 , C12Q1/68
CPC分类号： C12Q1/6895 , A01H1/04 , A01H5/10 , C07K14/415 , C12N15/8282 , C12Q2600/13 , C12Q2600/156 , C12Q2600/172
摘要： This invention relates to polynucleotide sequences encoding a gene that can confer resistance to the plant pathogen Colletotrichum, which causes anthracnose stalk rot, leaf blight and top dieback in corn and other cereals. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which enhance or confer resistance to the plant pathogen Colletotrichum, and processes of making said plants and seeds. The invention further presents sequences that can be used as molecular markers that in turn can be used to identify the region of interest in corn lines resulting from new crosses and to quickly and efficiently introgress the gene from corn lines carrying said gene into other corn lines that do not carry said gene, in order to make them resistant to Colletotrichum and resistant to stalk rot.
摘要翻译：本发明涉及编码能够赋予植物病原体Colletotrichum的抗性的基因的多核苷酸序列，其导致炭疽病腐烂，叶枯病和玉米和其他谷物中的顶部枯萎病。它进一步涉及携带包含所述多核苷酸序列的嵌合基因的植物的植物和种子，其增强或赋予对植物病原体Colletotrichum的抗性，以及制备所述植物和种子的过程。本发明还提供可用作分子标记的序列，其又可用于鉴定由新杂交产生的玉米品系中的感兴趣区域，并快速且有效地将携带所述基因的玉米品系的基因从其它玉米品系不携带所述基因，以使它们抵抗Colletotrichum并且抵抗茎腐烂。

7. 发明申请

WO2014165066A2 IDENTIFICATION OF P. PACHYRHIZI PROTEIN EFFECTORS AND THEIR USE IN PRODUCING ASIAN SOYBEAN RUST (ASR) RESISTANT PLANTS 审中-公开
标题翻译： P.PACHYRHIZI蛋白质效应物的鉴定及其在生产亚洲大豆抗性植物中的应用
公开(公告)号：WO2014165066A2
公开(公告)日：2014-10-09
申请号：PCT/US2014/024286
申请日：2014-03-12
申请人： E. I. DUPONT DE NEMOURS & COMPANY , BROGLIE, Karen E. , RAIRDAN, Gregory J.
发明人： BROGLIE, Karen E. , RAIRDAN, Gregory J.
IPC分类号： C07K14/37
CPC分类号： C12N15/8282 , C07K14/37 , C07K14/375
摘要： The disclosure relates to novel nucleic acids and their encoded polypeptides from ASR and methods of use that enhance the plant's defensive elicitation response.
摘要翻译：本公开涉及来自ASR的新型核酸及其编码多肽和增强植物防御性引发反应的使用方法。

8. 发明申请

WO2006107931A2 POLYNUCLEOTIDES AND METHODS FOR MAKING PLANTS RESISTANT TO FUNGAL PATHOGENS 审中-公开
标题翻译：多核苷酸和制备抗真菌病原体的植物的方法
公开(公告)号：WO2006107931A2
公开(公告)日：2006-10-12
申请号：PCT/US2006/012443
申请日：2006-04-04
申请人： E. I. DU PONT DE NEMOURS & COMPANY , PIONEER HI-BRED INTERNATIONAL, INC. , UNIVERSITY OF DELAWARE , BROGLIE, Karen E. , BUTLER, Karlene H. , BUTRUILLE, Marymar G. , DA SILVA CONCEICAO, Alexandre , FREY, Travis J , HAWK, James A. , JAQUETH, Jennifer S. , JONES, Elizabeth S. , MULTANI, Dilbag S. , WOLTERS, Petra J.
发明人： BROGLIE, Karen E. , BUTLER, Karlene H. , BUTRUILLE, Marymar G. , DA SILVA CONCEICAO, Alexandre , FREY, Travis J , HAWK, James A. , JAQUETH, Jennifer S. , JONES, Elizabeth S. , MULTANI, Dilbag S. , WOLTERS, Petra J.
IPC分类号： C12N15/82 , C12N15/29 , A01H5/00 , C12Q1/68
CPC分类号： C12Q1/6895 , A01H1/04 , A01H5/10 , C07K14/415 , C12N15/8282 , C12Q2600/13 , C12Q2600/156 , C12Q2600/172
摘要： This invention relates to polynucleotide sequences encoding a gene that can confer resistance to the plant pathogen Colletotrichum, which causes anthracnose stalk rot, leaf blight and top dieback in corn and other cereals. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which enhance or confer resistance to the plant pathogen Colletotrichum, and processes of making said plants and seeds. The invention further presents sequences that can be used as molecular markers that in turn can be used to identify the region of interest in corn lines resulting from new crosses and to quickly and efficiently introgress the gene from corn lines carrying said gene into other corn lines that do not carry said gene, in order to make them resistant to Colletotrichum and resistant to stalk rot.
摘要翻译：本发明涉及编码可赋予对植物病原体Colletotrichum抗性的基因的多核苷酸序列，所述植物病原体Colletotrichum在玉米和其他谷类中引起炭疽病茎腐病，叶枯病和顶部枯萎病。本发明还涉及携带嵌合基因的植物和植物种子以及制备所述植物和种子的过程，所述嵌合基因包含增强或赋予对植物病原体Colletotrichum的抗性的所述多核苷酸序列。本发明进一步提供了可以用作分子标记的序列，所述分子标记又可以用于鉴定由新杂交产生的玉米品系中感兴趣的区域，并且快速且有效地将来自携带所述基因的玉米品系的基因渗入到其他玉米品系中，不要携带所述基因，以使它们对Colletotrichum具有抗性并且抵抗茎腐病。

9. 发明申请

WO9722703A3 NOVEL STARCHES VIA MODIFICATION OF EXPRESSION OF STARCH BIOSYNTHETIC ENZYME GENES 审中-公开
标题翻译：通过淀粉酶生物合成酶基因表达修饰的新方法
公开(公告)号：WO9722703A3
公开(公告)日：1997-07-24
申请号：PCT/US9619678
申请日：1996-12-12
申请人： DU PONT , HUBBARD NATALIE LOUISE , KLEIN THEODORE MITCHELL , BROGLIE KAREN E
发明人： HUBBARD NATALIE LOUISE , KLEIN THEODORE MITCHELL , BROGLIE KAREN E
IPC分类号： A23L1/0522 , C12N9/10 , C12N15/29 , C12N15/54 , C12N15/82 , A01H5/00 , C08B30/00
CPC分类号： C12N9/107 , A23L29/212 , C12N15/8245
摘要： The instant invention discloses utilization of a cDNA clone to construct sense and antisense genes for inhibition of starch branching enzyme enzymatic activity in corn. More specifically, this invention concerns a method of controlling the starch fine structure of starch derived from the grain of corn comprising: (1) preparing a chimeric gene comprising a nucleic acid fragment encoding a starch branching enzyme structural gene or a fragment thereof, operably linked in either sense or antisense orientation on the upstream side to a nucleic acid fragment encoding a promoter that directs gene expression in corn endosperm tissue, and operably linked on the downstream side to a nucleic acid fragment encoding a suitable regulatory sequence for transcriptional termination, and (2) transforming corn with said chimeric gene, wherein expression of said chimeric gene results in alteration of the fine structure of starch derived from the grain of said transformed corn compared to the fine structure of starch derived from corn not possessing said chimeric gene.

10. 发明申请

WO2014165066A3 IDENTIFICATION OF P. PACHYRHIZI PROTEIN EFFECTORS AND THEIR USE IN PRODUCING ASIAN SOYBEAN RUST (ASR) RESISTANT PLANTS 审中-公开
标题翻译： P. PACHYRHIZI蛋白质效应物的鉴定及其在亚洲大豆抗锈病生产中的应用
公开(公告)号：WO2014165066A3
公开(公告)日：2014-11-27
申请号：PCT/US2014024286
申请日：2014-03-12
申请人： DU PONT , BROGLIE KAREN E , RAIRDAN GREGORY J
发明人： BROGLIE KAREN E , RAIRDAN GREGORY J
IPC分类号： C07K14/37 , C12N15/82
CPC分类号： C12N15/8282 , C07K14/37 , C07K14/375
摘要： The disclosure relates to novel nucleic acids and their encoded polypeptides from ASR and methods of use that enhance the plant's defensive elicitation response.
摘要翻译：本公开涉及来自ASR的新型核酸及其编码的多肽和增强植物的防御诱导反应的使用方法。

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