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1. 发明申请

WO2003057174A3 METHODS AND COMPOSITIONS FOR PROTEIN EXPRESSION AND PURIFICATION 审中-公开
公开(公告)号：WO2003057174A3
公开(公告)日：2003-07-17
申请号：PCT/US2003/000436
申请日：2003-01-07
申请人： LIFESENSORS, INC. , BUTT, Tauseef, R. , WEEKS, Steven, D. , TRAN, Hiep, T. , MALAKHOV, Michael, P. , MALAKHOVA, Oxana, A.
发明人： BUTT, Tauseef, R. , WEEKS, Steven, D. , TRAN, Hiep, T. , MALAKHOV, Michael, P. , MALAKHOVA, Oxana, A.
IPC分类号： C12P21/06
摘要： Methods for enhancing expression levels and secretion of heterologous fusion proteins in a host cell are disclosed.

2. 发明申请

WO2010039692A3 TRANSFORMED SACCHAROMYCES CEREVISIAE ENGINEERED FOR XYLOSE UTILIZATION 审中-公开
标题翻译：用于XYLOSE利用的改造的SACCHAROMYCES CERSEISY
公开(公告)号：WO2010039692A3
公开(公告)日：2010-07-01
申请号：PCT/US2009058749
申请日：2009-09-29
申请人： US AGRICULTURE , HUGHES STEPHEN R , BUTT TAUSEEF R
发明人： HUGHES STEPHEN R , BUTT TAUSEEF R
IPC分类号： C12N1/19 , C12N15/52 , C12R1/865
CPC分类号： C12N1/18 , C12P7/06 , C12P7/10 , C12R1/865 , Y02E50/16 , Y02E50/17
摘要： Recombinant Saccharomyces cerevisiae produced by transformation with heterologous polynucleotide sequences coding for xylulokinase (XKS) from Yersinia pestis and xylose isomerase (XI) are capable of xylose utilization. The transformants express these heterologous polynucleotides at a sufficient functional level to grow aerobically on xylose as the sole carbon source. Further transformation of the recombinant yeasts to overexpress one or more of the S cerevisiae genes PIP2, IMG2, MAK5, VPS9, COXlO, ALEl, CDC7, and MMS4, permits the yeast to grow anaerobically on xylose as the sole carbon source. When grown under anaerobic conditions on a culture medium comprising both glucose and xylose, the transformed yeast exhibit increased ethanol productivity, with the yeast growing on the xylose to increase their biomass and fermenting the glucose to ethanol.
摘要翻译：通过用编码来自鼠疫耶尔森疫霉和木糖异构酶（XI）的木酮糖激酶（XKS）的异源多核苷酸序列转化产生的重组酿酒酵母能够利用木糖。转化体以足够的功能水平表达这些异源多核苷酸，以在木糖上作为唯一碳源有氧生长。进一步转化重组酵母过表达一种或多种酿酒酵母基因PIP2，IMG2，MAK5，VPS9，COX10，ALE1，CDC7和MMS4，允许酵母作为唯一碳源在木糖上厌氧生长。当在厌氧条件下在包含葡萄糖和木糖的培养基上生长时，转化的酵母显示提高的乙醇生产力，酵母生长在木糖上以增加其生物量并将葡萄糖发酵成乙醇。

3. 发明申请

WO2008083271A3 METHODS AND COMPOSITIONS FOR ENHANCED PROTEIN EXPRESSION AND PURIFICATION 审中-公开
标题翻译：增强蛋白质表达和纯化的方法和组合物
公开(公告)号：WO2008083271A3
公开(公告)日：2008-11-20
申请号：PCT/US2007089035
申请日：2007-12-28
申请人： LIFESENSORS INC , BUTT TAUSEEF R , PANAVAS TADAS , KARWA AMOLKUMAR , PEROUTKA RAYMOND J , MARBLESTONE JEFFREY G
发明人： BUTT TAUSEEF R , PANAVAS TADAS , KARWA AMOLKUMAR , PEROUTKA RAYMOND J , MARBLESTONE JEFFREY G
IPC分类号： C12N15/63 , C07K14/00 , C12N15/85 , C12P21/00
CPC分类号： C12P21/02 , C07K14/00 , C07K14/4702 , C07K2319/00 , C07K2319/20
摘要： Methods for enhancing expression levels, secretion, and purification of heterologous fusion proteins in a host cell are disclosed.
摘要翻译：公开了用于增强宿主细胞中异源融合蛋白的表达水平，分泌和纯化的方法。

4. 发明申请

WO2003057174A2 METHODS AND COMPOSITIONS FOR PROTEIN EXPRESSION AND PURIFICATION 审中-公开
标题翻译：蛋白质表达和纯化的方法和组合物
公开(公告)号：WO2003057174A2
公开(公告)日：2003-07-17
申请号：PCT/US2003/000436
申请日：2003-01-07
申请人： LIFESENSORS, INC. , BUTT, Tauseef, R. , WEEKS, Steven, D. , TRAN, Hiep, T. , MALAKHOV, Michael, P. , MALAKHOVA, Oxana, A.
发明人： BUTT, Tauseef, R. , WEEKS, Steven, D. , TRAN, Hiep, T. , MALAKHOV, Michael, P. , MALAKHOVA, Oxana, A.
IPC分类号： A61K
CPC分类号： C12P21/02 , C07K14/00 , C07K2319/02 , C07K2319/21 , C07K2319/60 , C07K2319/95 , C12N15/62
摘要： Methods for enhancing expression levels and secretion of heterologous fusion proteins in a host cell are disclosed.
摘要翻译：公开了用于增强宿主细胞中异源融合蛋白的表达水平和分泌的方法。

5. 发明申请

WO2005003313A8 Methods for enhancing expression levels and secretion of heterologous fusion proteins in a host cell are disclosed. 审中-公开
标题翻译：公开了用于增强宿主细胞中异源融合蛋白的表达水平和分泌的方法。
公开(公告)号：WO2005003313A8
公开(公告)日：2006-07-06
申请号：PCT/US2004020778
申请日：2004-06-28
申请人： LIFESENSORS INC , BUTT TAUSEEF R , MALAKHOV MICHAEL P , MALAKHOVA OXANA A
发明人： BUTT TAUSEEF R , MALAKHOV MICHAEL P , MALAKHOVA OXANA A
IPC分类号： C07K14/00 , C12N20060101 , C12N15/09 , C12N15/62 , C12N15/67 , C12P21/02 , C12P21/04 , C12P21/06
CPC分类号： C12P21/02 , C07K14/00 , C07K2319/35 , C07K2319/50 , C12N15/62 , C12N15/67
摘要： Methods for enhancing expression levels and secretion of heterologous fusion proteins in a host cell are disclosed.
摘要翻译：公开了用于增强宿主细胞中异源融合蛋白的表达水平和分泌的方法。

6. 发明申请

WO2006002100A2 DIAGNOSTIC AND SCREENING METHODS AND KITS ASSOCIATED WITH PROTEOLYTIC ACTIVITY 审中-公开
标题翻译：诊断和筛查方法以及与蛋白活性相关的试剂盒
公开(公告)号：WO2006002100A2
公开(公告)日：2006-01-05
申请号：PCT/US2005/021819
申请日：2005-06-21
申请人： PROGENRA INC. , BUTT, Tauseef, R. , BERNAL, Alejandro
发明人： BUTT, Tauseef, R. , BERNAL, Alejandro
IPC分类号： C12Q1/37
CPC分类号： G01N33/573 , C12Q1/37 , G01N2333/916
摘要： Methods and kits for assessing proteolytic enzyme activity and a modulator’s effect thereof employ a Ubiquitin or Ubiquitin-like Protein and a signal producing structure. The corresponding fusion polynucleotide is employed for production of transgenic cells, plants and animals that may be produced by stably transfection, optionally transforming the cell, plant or animal with a Ubiquitin-,UBL-or their C-terminal binding functional fragment-Reporter fusion polynucleotide. A method of diagnosing a disease or condition, comprises contacting or administering a sample obtained from a subject suspected of being afflicted with the disease or condition with the cell, plant or animal, detecting any signal produced by the reporter in the presence of the sample and comparing the signal to controls for 0% and 100% signals.
摘要翻译：用于评估蛋白水解酶活性和其调节剂效应的方法和试剂盒使用泛素或泛素样蛋白和信号产生结构。相应的融合多核苷酸用于产生可通过稳定转染产生的转基因细胞，植物和动物，任选地用泛素 - ，UBL-或其C-末端结合功能片段 - 报道子融合多核苷酸转化细胞，植物或动物。诊断疾病或病症的方法包括将从怀疑患有疾病或病症的受试者获得的样品与细胞，植物或动物接触或施用，检测在样品存在下由报道分子产生的任何信号，和将信号与控制0％和100％信号进行比较。

7. 发明申请

WO2005003313A3 METHODS AND COMPOSITIONS FOR ENHANCED PROTEIN EXPRESSION AND PURIFICATION 审中-公开
标题翻译：增强蛋白质表达和纯化的方法和组合物
公开(公告)号：WO2005003313A3
公开(公告)日：2005-07-21
申请号：PCT/US2004020778
申请日：2004-06-28
申请人： LIFESENSORS INC , BUTT TAUSEEF R , MALAKHOV MICHAEL P , MALAKHOVA OXANA A
发明人： BUTT TAUSEEF R , MALAKHOV MICHAEL P , MALAKHOVA OXANA A
IPC分类号： C12N15/09 , C07K14/00 , C12N20060101 , C12N15/62 , C12N15/67 , C12P21/02 , C12P21/04 , C12P21/06
CPC分类号： C12P21/02 , C07K14/00 , C07K2319/35 , C07K2319/50 , C12N15/62 , C12N15/67
摘要： Methods for enhancing expression levels and secretion of heterologous fusion proteins in a host cell are disclosed.
摘要翻译：公开了用于增强宿主细胞中表达水平和异源融合蛋白分泌的方法。

8. 发明申请

WO2010039692A2 TRANSFORMED SACCHAROMYCES CEREVISIAE ENGINEERED FOR XYLOSE UTILIZATION 审中-公开
标题翻译：为木糖利用而改造的酿酒酵母
公开(公告)号：WO2010039692A2
公开(公告)日：2010-04-08
申请号：PCT/US2009/058749
申请日：2009-09-29
申请人： THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY OF AGRICULTURE , HUGHES, Stephen, R. , BUTT, Tauseef, R.
发明人： HUGHES, Stephen, R. , BUTT, Tauseef, R.
IPC分类号： C12N1/19 , C12N15/52 , C12R1/865
CPC分类号： C12N1/18 , C12P7/06 , C12P7/10 , C12R1/865 , Y02E50/16 , Y02E50/17
摘要： Recombinant Saccharomyces cerevisiae produced by transformation with heterologous polynucleotide sequences coding for xylulokinase (XKS) from Yersinia pestis and xylose isomerase (XI) are capable of xylose utilization. The transformants express these heterologous polynucleotides at a sufficient functional level to grow aerobically on xylose as the sole carbon source. Further transformation of the recombinant yeasts to overexpress one or more of the S cerevisiae genes PIP2, IMG2, MAK5, VPS9, COXlO, ALEl, CDC7, and MMS4, permits the yeast to grow anaerobically on xylose as the sole carbon source. When grown under anaerobic conditions on a culture medium comprising both glucose and xylose, the transformed yeast exhibit increased ethanol productivity, with the yeast growing on the xylose to increase their biomass and fermenting the glucose to ethanol.
摘要翻译：通过用编码来自鼠疫耶尔森氏菌的木酮糖激酶（XKS）和木糖异构酶（XI）的异源多核苷酸序列转化而产生的重组酿酒酵母能够木糖利用。转化体以足够的功能水平表达这些异源多核苷酸以在木糖上作为唯一碳源好氧生长。进一步转化重组酵母以过表达一种或多种酿酒酵母基因PIP2，IMG2，MAK5，VPS9，COX10，ALE1，CDC7和MMS4，允许酵母在木糖上无氧生长，如唯一碳源。当在厌氧条件下在包含葡萄糖和木糖的培养基上生长时，转化的酵母表现出提高的乙醇生产力，酵母在木糖上生长以增加其生物量并将葡萄糖发酵成乙醇。

9. 发明申请

WO2008083271A2 METHODS AND COMPOSITIONS FOR ENHANCED PROTEIN EXPRESSION AND PURIFICATION 审中-公开
标题翻译：用于增强蛋白质表达和纯化的方法和组合物
公开(公告)号：WO2008083271A2
公开(公告)日：2008-07-10
申请号：PCT/US2007/089035
申请日：2007-12-28
申请人： LIFESENSORS, INC. , BUTT, Tauseef, R. , PANAVAS, Tadas , KARWA, Amolkumar , PEROUTKA, Raymond, J. , MARBLESTONE, Jeffrey, G.
发明人： BUTT, Tauseef, R. , PANAVAS, Tadas , KARWA, Amolkumar , PEROUTKA, Raymond, J. , MARBLESTONE, Jeffrey, G.
IPC分类号： C12P21/06
CPC分类号： C12P21/02 , C07K14/00 , C07K14/4702 , C07K2319/00 , C07K2319/20
摘要： Methods for enhancing expression levels, secretion, and purification of heterologous fusion proteins in a host cell are disclosed.
摘要翻译：公开了用于增强宿主细胞中异源融合蛋白的表达水平，分泌和纯化的方法。

10. 发明申请

WO2006002100A3 DIAGNOSTIC AND SCREENING METHODS AND KITS ASSOCIATED WITH PROTEOLYTIC ACTIVITY 审中-公开
标题翻译：诊断和筛选方法和与蛋白质活性相关的试剂盒
公开(公告)号：WO2006002100A3
公开(公告)日：2006-04-20
申请号：PCT/US2005021819
申请日：2005-06-21
申请人： PROGENRA INC , BUTT TAUSEEF R , BERNAL ALEJANDRO
发明人： BUTT TAUSEEF R , BERNAL ALEJANDRO
IPC分类号： G01N33/53 , C12Q1/37
CPC分类号： G01N33/573 , C12Q1/37 , G01N2333/916
摘要： Methods and kits for assessing proteolytic enzyme activity and a modulator's effect thereof employ a Ubiquitin or Ubiquitin-like Protein and a signal producing structure. The corresponding fusion polynucleotide is employed for production of transgenic cells, plants and animals that may be produced by stably transfection, optionally transforming the cell, plant or animal with a Ubiquitin-,UBL-or their C-terminal binding functional fragment-Reporter fusion polynucleotide. A method of diagnosing a disease or condition, comprises contacting or administering a sample obtained from a subject suspected of being afflicted with the disease or condition with the cell, plant or animal, detecting any signal produced by the reporter in the presence of the sample and comparing the signal to controls for 0% and 100% signals.
摘要翻译：用于评估蛋白水解酶活性的方法和试剂盒及其调节剂的作用采用泛素或泛素样蛋白和信号产生结构。相应的融合多核苷酸用于生产可通过稳定转染产生的转基因细胞，植物和动物，任选地用泛素 - ，UBL-或其C-末端结合功能片段 - 报道融合多核苷酸转化细胞，植物或动物。一种诊断疾病或病症的方法包括将从疑似患有疾病或病症的受试者获得的样品与细胞，植物或动物接触或施用，在样品存在下检测报告物产生的任何信号，以及将信号与0％和100％信号的控制进行比较。

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