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    • 2. 发明申请
    • THE INDUCTION OF STRESS-RELATED FACTORS IN PLANTS
    • 植物中胁迫相关因子的诱导
    • WO1999004013A2
    • 1999-01-28
    • PCT/US1998014861
    • 1998-07-17
    • PIONEER HI-BRED INTERNATIONAL, INC.ALTIER, Daniel, J.BIDNEY, Dennis, L.COUGHLAN, Sean, J.FALAK, IgorYALPANI, NasserLU, GuihauMANCL, Mark, K.NAZARIAN, Katherine, A., P.SCELONGE, Christopher, J.
    • PIONEER HI-BRED INTERNATIONAL, INC.
    • C12N15/53
    • C12N9/0008C12N9/0006C12N15/8271C12N15/8282C12Q1/26
    • The present invention relates to a method of producing resistance to stress in a variety of plants by the expression of a hydrogen peroxide or reactive oxygen species producing enzyme such as, but not limited to, oxalate oxidase. In addition, the present invention relates to a method of producing a stress resistant sunflower plant by transforming a sunflower plant with an oxalate oxidase gene or other hydrogen peroxide/reactive oxygen species producing genes that upon expression of the gene produce an enzyme capable of generating hydrogen peroxide or reactive oxygen species from endogeously available substrate. Alternatively, the plant could be transformed with the hydrogen peroxide or reactive oxygen species producing gene and a gene for the necessary substrate or a gene which would modify a pathway in the plant resulting in formation or increase in substrate. The production of hydrogen peroxide or a reactive oxygen species triggers the expression of hydrogen peroxide/reactive oxygen species-responsive genes resulting ina pre-challenge accumulation of pathogenesis or stress-related factors such as salicylic acid and PR protein. Thus the plant is rendered resistant to a variety of stresses by, for example, inhibiting the establishment of a pathogen resulting in a disease resistance phenotype.
    • 本发明涉及通过表达产生酶如草酸盐的过氧化氢或活性氧种类在各种植物中产生胁迫抗性的方法 但不仅如此。 另外,本发明涉及使用草酸氧化酶基因或其他产生过氧化氢的基因/物种通过向日葵的转化来制造对抗胁迫的向日葵的方法 活性氧在表达后产生能够从可利用的内源性底物产生过氧化氢或活性氧物种的酶。 根据另一个实施方案,所述植物可以通过产生过氧化氢的基因或活性氧的种类以及通过作用于必需底物的基因或通过改变植物过程的基因来转化。 从而导致所述衬底的形成或扩大。 过氧化氢或活性氧的产生触发了对这些物质表达敏感的基因的表达,导致发病前或应激相关因素的预先积聚,例如 比水杨酸和PR蛋白(与发病有关)。 因此,通过例如抑制产生疾病抗性表型的病原体的建立使植物抵抗多种胁迫。