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1. 发明申请

WO0032792A2 PLANT 1-DEOXY-D-XYLULOSE 5-PHOSPHATE SYNTHASE 审中-公开
标题翻译：植物1-脱氧-D-木糖5-磷酸合成酶
公开(公告)号：WO0032792A2
公开(公告)日：2000-06-08
申请号：PCT/US9928587
申请日：1999-12-02
申请人： DU PONT , CAHOON REBECCA E , TAO YONG , WILLIAMS MARK E , COUGHLAN SEAN J , WENG ZUDE
发明人： CAHOON REBECCA E , TAO YONG , WILLIAMS MARK E , COUGHLAN SEAN J , WENG ZUDE
IPC分类号： C12N9/10 , C12N15/29 , C12N15/54 , C12N15/82 , C12N5/10 , C12Q1/48 , C12Q1/68
CPC分类号： C12N9/1022 , C12N15/8243 , C12N15/8274
摘要： This invention relates to an isolated nucleic acid fragment encoding an isopentenyl diphosphate biosynthetic enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the isopentenyl diphosphate biosynthetic enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the isopentenyl diphosphate biosynthetic enzyme in a transformed host cell.
摘要翻译：本发明涉及编码异戊烯基二磷酸生物合成酶的分离的核酸片段。本发明还涉及以正义或反义方向构建编码全部或部分异戊烯基二磷酸酯生物合成酶的嵌合基因，其中嵌合基因的表达导致转化的异戊烯基二磷酸酯生物合成酶水平的改变宿主细胞。

2. 发明申请

WO9904013A3 THE INDUCTION OF STRESS-RELATED FACTORS IN PLANTS 审中-公开
标题翻译：植物中应力相关因素的诱导
公开(公告)号：WO9904013A3
公开(公告)日：1999-05-14
申请号：PCT/US9814861
申请日：1998-07-17
申请人： PIONEER HI BRED INT , ALTIER DANIEL J , BIDNEY DENNIS L , COUGHLAN SEAN J , FALAK IGOR , YALPANI NASSER , LU GUIHAU , MANCL MARK K , NAZARIAN KATHERINE A P , SCELONGE CHRISTOPHER J
发明人： ALTIER DANIEL J , BIDNEY DENNIS L , COUGHLAN SEAN J , FALAK IGOR , YALPANI NASSER , LU GUIHAU , MANCL MARK K , NAZARIAN KATHERINE A P , SCELONGE CHRISTOPHER J
IPC分类号： C12N9/02 , C12N9/04 , C12N15/53 , C12N15/82 , C12Q1/26 , A01H5/00 , C12N5/10
CPC分类号： C12N9/0008 , C12N9/0006 , C12N15/8271 , C12N15/8282 , C12Q1/26
摘要： The present invention relates to a method of producing resistance to stress in a variety of plants by the expression of a hydrogen peroxide or reactive oxygen species producing enzyme such as, but not limited to, oxalate oxidase. In addition, the present invention relates to a method of producing a stress resistant sunflower plant by transforming a sunflower plant with an oxalate oxidase gene or other hydrogen peroxide/reactive oxygen species producing genes that upon expression of the gene produce an enzyme capable of generating hydrogen peroxide or reactive oxygen species from endogeously available substrate. Alternatively, the plant could be transformed with the hydrogen peroxide or reactive oxygen species producing gene and a gene for the necessary substrate or a gene which would modify a pathway in the plant resulting in formation or increase in substrate. The production of hydrogen peroxide or a reactive oxygen species triggers the expression of hydrogen peroxide/reactive oxygen species-responsive genes resulting ina pre-challenge accumulation of pathogenesis or stress-related factors such as salicylic acid and PR protein. Thus the plant is rendered resistant to a variety of stresses by, for example, inhibiting the establishment of a pathogen resulting in a disease resistance phenotype.
摘要翻译：本发明涉及通过表达过氧化氢或活性氧产生酶，例如但不限于草酸氧化酶，在各种植物中产生抗应力的方法。此外，本发明涉及一种通过用草酸氧化酶基因或其他过氧化氢/活性氧转化向日葵植物产生抗胁迫向日葵植物的方法，其产生基因表达时基因产生能产生氢的酶过氧化物或来自内在可用底物的活性氧。或者，可以用过氧化氢或活性氧产生基因和必需底物的基因或将修饰植物中途径导致底物形成或增加的基因转化植物。过氧化氢或活性氧的产生触发了过氧化氢/活性氧物质反应基因的表达，导致了攻击前积累的发病机制或应激相关因素如水杨酸和PR蛋白。因此，通过例如抑制导致抗病性表型的病原体的建立，使植物对各种胁迫具有抗性。

3. 发明申请

WO0032792A9 PLANT 1-DEOXY-D-XYLULOSE 5-PHOSPHATE SYNTHASE 审中-公开
标题翻译：植物1-脱氧-D-木糖醇5-磷酸酯合成酶
公开(公告)号：WO0032792A9
公开(公告)日：2001-04-19
申请号：PCT/US9928587
申请日：1999-12-02
申请人： DU PONT , CAHOON REBECCA E , TAO YONG , WILLIAMS MARK E , COUGHLAN SEAN J , WENG ZUDE
发明人： CAHOON REBECCA E , TAO YONG , WILLIAMS MARK E , COUGHLAN SEAN J , WENG ZUDE
IPC分类号： C12N9/10 , C12N15/29 , C12N15/54 , C12N15/82 , C12N5/10 , C12Q1/48 , C12Q1/68
CPC分类号： C12N9/1022 , C12N15/8243 , C12N15/8274
摘要： This invention relates to an isolated nucleic acid fragment encoding an isopentenyl diphosphate biosynthetic enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the isopentenyl diphosphate biosynthetic enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the isopentenyl diphosphate biosynthetic enzyme in a transformed host cell.
摘要翻译：本发明涉及编码异戊烯基二磷酸生物合成酶的分离的核酸片段。本发明还涉及在正义或反义方向上构建编码全部或部分异戊烯基二磷酸生物合成酶的嵌合基因，其中嵌合基因的表达导致转化的异戊烯基二磷酸生物合成酶水平的变化宿主细胞。

4. 发明申请

WO1999004013A2 THE INDUCTION OF STRESS-RELATED FACTORS IN PLANTS 审中-公开
标题翻译：植物中胁迫相关因子的诱导
公开(公告)号：WO1999004013A2
公开(公告)日：1999-01-28
申请号：PCT/US1998014861
申请日：1998-07-17
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , ALTIER, Daniel, J. , BIDNEY, Dennis, L. , COUGHLAN, Sean, J. , FALAK, Igor , YALPANI, Nasser , LU, Guihau , MANCL, Mark, K. , NAZARIAN, Katherine, A., P. , SCELONGE, Christopher, J.
发明人： PIONEER HI-BRED INTERNATIONAL, INC.
IPC分类号： C12N15/53
CPC分类号： C12N9/0008 , C12N9/0006 , C12N15/8271 , C12N15/8282 , C12Q1/26
摘要： The present invention relates to a method of producing resistance to stress in a variety of plants by the expression of a hydrogen peroxide or reactive oxygen species producing enzyme such as, but not limited to, oxalate oxidase. In addition, the present invention relates to a method of producing a stress resistant sunflower plant by transforming a sunflower plant with an oxalate oxidase gene or other hydrogen peroxide/reactive oxygen species producing genes that upon expression of the gene produce an enzyme capable of generating hydrogen peroxide or reactive oxygen species from endogeously available substrate. Alternatively, the plant could be transformed with the hydrogen peroxide or reactive oxygen species producing gene and a gene for the necessary substrate or a gene which would modify a pathway in the plant resulting in formation or increase in substrate. The production of hydrogen peroxide or a reactive oxygen species triggers the expression of hydrogen peroxide/reactive oxygen species-responsive genes resulting ina pre-challenge accumulation of pathogenesis or stress-related factors such as salicylic acid and PR protein. Thus the plant is rendered resistant to a variety of stresses by, for example, inhibiting the establishment of a pathogen resulting in a disease resistance phenotype.
摘要翻译：本发明涉及通过表达产生酶如草酸盐的过氧化氢或活性氧种类在各种植物中产生胁迫抗性的方法但不仅如此。另外，本发明涉及使用草酸氧化酶基因或其他产生过氧化氢的基因/物种通过向日葵的转化来制造对抗胁迫的向日葵的方法活性氧在表达后产生能够从可利用的内源性底物产生过氧化氢或活性氧物种的酶。根据另一个实施方案，所述植物可以通过产生过氧化氢的基因或活性氧的种类以及通过作用于必需底物的基因或通过改变植物过程的基因来转化。从而导致所述衬底的形成或扩大。过氧化氢或活性氧的产生触发了对这些物质表达敏感的基因的表达，导致发病前或应激相关因素的预先积聚，例如比水杨酸和PR蛋白（与发病有关）。因此，通过例如抑制产生疾病抗性表型的病原体的建立使植物抵抗多种胁迫。

5. 发明申请

WO0032757A3 PLANT VITAMIN E BIOSYNTHETIC ENZYMES 审中-公开
标题翻译：植物维生素E生物合成酶
公开(公告)号：WO0032757A3
公开(公告)日：2000-11-23
申请号：PCT/US9928588
申请日：1999-12-02
申请人： DU PONT , CAHOON REBECCA E , COUGHLAN SEAN J , MIAO GUO HUA , RAFALSKI J ANTONI
发明人： CAHOON REBECCA E , COUGHLAN SEAN J , MIAO GUO-HUA , RAFALSKI J ANTONI
IPC分类号： C12N9/02 , C12N9/10 , C12N15/52 , C12N15/82 , C12N15/29 , C12N15/63 , C12N15/83 , C12Q1/26 , C12Q1/48 , C12Q1/68 , G01N33/50
CPC分类号： C12N9/0069 , C12N9/1007 , C12N15/52 , C12N15/8243
摘要： This invention relates to an isolated nucleic acid fragment encoding a vitamin E biosynthetic enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the vitamin E biosynthetic enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the vitamin E biosynthetic enzyme in a transformed host cell.
摘要翻译：本发明涉及编码维生素E生物合成酶的分离的核酸片段。本发明还涉及在正义或反义方向上编码全部或部分维生素E生物合成酶的嵌合基因的构建，其中嵌合基因的表达导致转化的维生素E生物合成酶水平的改变宿主细胞。

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