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1. 发明专利

JP2005013164A Vegetable virus vector 有权
标题翻译：蔬菜病毒载体
公开(公告)号：JP2005013164A
公开(公告)日：2005-01-20
申请号：JP2003185981
申请日：2003-06-27
申请人： Hokkaido Green Bio Kenkyusho:Kk , Osamu Masuda , National Institute Of Advanced Industrial & Technology , 税増田 , 株式会社北海道グリーンバイオ研究所 , 独立行政法人産業技術総合研究所
发明人： MATSUMURA TAKESHI , MASUDA OSAMU , ITSUCHIYOUDA NORIKO
IPC分类号： C12N15/09 , C12N5/10 , C12N15/82 , C12N15/83
CPC分类号： C12N15/8203
摘要： PROBLEM TO BE SOLVED: To provide a new vegetable virus vector and a method for the expression of vegetable gene. SOLUTION: The invention provides a vegetable virus vector produced by depleting a part of the 2b region of the RNA2 molecule of cucumber mosaic virus (CMV), the vegetable virus vector produced by depleting the region from the StuI site to the stop codon of 2b ORF of pCY2 which is an infective clone of CMV-Y RNA2, and a method for the expression of a gene of a vegetable by transferring an exogenote to the vegetable using the recombinant gene to stably express the exogenote. The vector is useful as a vegetable RNA vector to effect the stable expression of the exogenote. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供新的植物病毒载体和表达植物基因的方法。解决方案：本发明提供了通过消耗黄瓜花叶病毒（CMV）的RNA2分子的2b区的一部分产生的植物病毒载体，所述植物病毒载体是通过将从StuI位点到终止密码子的区域耗尽而产生的植物病毒载体作为CMV-Y RNA2的感染性克隆的pCY2的2b ORF，以及通过使用重组基因将外源基因转移到植物以稳定表达外源基因来表达植物基因的方法。载体可用作植物RNA载体以实现外源的稳定表达。版权所有（C）2005，JPO＆NCIPI

2. 发明专利

JP2004113007A Method for selecting wheat 审中-公开
标题翻译：选择小麦的方法
公开(公告)号：JP2004113007A
公开(公告)日：2004-04-15
申请号：JP2002276822
申请日：2002-09-24
申请人： Hokkaido Green Bio Kenkyusho:Kk , 株式会社北海道グリーンバイオ研究所
发明人： TAKEKIDA JUNJI
IPC分类号： A01H1/00 , C12N15/09 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To provide a method for selecting wheat accompanied with seeds having high dormancy by which a genetic marker, especially an amplified genetic marker for a gene involved in the dormancy of the wheat is found out in order to stably, efficiently and simply select the dormancy of individuals of the wheat and the genetic marker is used.
SOLUTION: The method for selecting the wheat is carried out so as to select the wheat accompanied with the seeds having the high dormancy by using the genetic marker in a region connected to a gene imparting the high dormancy to the seeds of the wheat as an index.
COPYRIGHT: (C)2004,JPO
摘要翻译：待解决的问题：提供一种选择具有高休眠种子的小麦的方法，通过该方法，发现遗传标记，特别是发现涉及小麦休眠的基因的扩增遗传标记，以便稳定地，有效和简单地选择小麦个体的休眠，并使用遗传标记。解决方案：进行选择小麦的方法，通过在连接到赋予高休眠的基因的区域中使用遗传标记来选择伴随着具有高休眠的种子的小麦对小麦的种子作为指标。版权所有（C）2004，JPO

3. 发明专利

JP2005117979A Primer set for detecting dna marker linking to low molecular weight glutenin gene of wheat, and use thereof 有权
标题翻译：用于检测连接到低分子量谷蛋白基因的DNA标记的初步设置及其使用
公开(公告)号：JP2005117979A
公开(公告)日：2005-05-12
申请号：JP2003357538
申请日：2003-10-17
申请人： Hokkaido Green Bio Kenkyusho:Kk , National Agriculture & Bio-Oriented Research Organization , 株式会社北海道グリーンバイオ研究所 , 独立行政法人農業・生物系特定産業技術研究機構
发明人： FUNATSUKI WAKAKO , TAKADA KANENORI , YAMAUCHI HIROAKI , TABIKI TADASHI , NISHIO ZENTA , SARUYAMA HARUO , HACHIMAN ERIKO
IPC分类号： A01H5/00 , C12N15/09 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To provide a primer set for detecting a DNA marker linking to a 42 kDa low molecular weight glutenin gene capable of imparting strength to wheat; and to provide the DNA marker linking to the gene, and a method for using them. SOLUTION: The primer set capable of detecting the DNA marker linked to the 42 kDa low molecular weight glutenin gene of the wheat is constituted of an oligonucleotide comprising a base sequence represented by a specific sequence, and an oligonucleotide comprising a base sequence represented by another specified sequence. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供用于检测连接到能够赋予小麦强度的42kDa低分子量麦谷蛋白基因的DNA标记的引物组; 并提供与该基因连接的DNA标记及其使用方法。解决方案：能够检测与小麦的42kDa低分子量麦谷蛋白基因连接的DNA标记的引物组由包含由特定序列表示的碱基序列的寡核苷酸构成，并且含有表示的碱基序列的寡核苷酸通过另一个指定的序列。版权所有（C）2005，JPO＆NCIPI

4. 发明专利

JP2005069935A Variety determination method for raw wheat for wheat flour 审中-公开
标题翻译：用于小麦胚芽的变异测定方法
公开(公告)号：JP2005069935A
公开(公告)日：2005-03-17
申请号：JP2003301455
申请日：2003-08-26
申请人： Ebetsu Seifun Kk , Hokkaido Green Bio Kenkyusho:Kk , National Agriculture & Bio-Oriented Research Organization , 株式会社北海道グリーンバイオ研究所 , 江別製粉株式会社 , 独立行政法人農業・生物系特定産業技術研究機構
发明人： SARUYAMA HARUO , HACHIMAN ERIKO , TANIDA MASATOSHI , FUNATSUKI WAKAKO , TABIKI TADASHI , NISHIO ZENTA , TAKADA KANENORI , YAMAMOTO YOSHIHIKO
IPC分类号： G01N33/10 , G01N27/447
摘要： PROBLEM TO BE SOLVED: To provide a method of determining accurately, easily and quickly the variety of raw wheat for wheat flour. SOLUTION: This method of determining the variety of the raw wheat for the wheat flour includes the following processes for: (a) extracting proteins from the wheat flour to prepare the protein sample; (b) separating the obtained protein sample by a two-dimensional electrophoretic method; (c) visualizing a separation pattern of the protein sample on gel; and (d) determining the variety of the raw wheat for the wheat flour, based on the visualized separation pattern of the proteins. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供一种准确，容易，准确地确定小麦面粉品种的方法。解决方案：这种确定小麦面粉原料种类的方法包括以下步骤：（a）从小麦粉中提取蛋白质以制备蛋白质样品; （b）通过二维电泳法分离获得的蛋白质样品; （c）在凝胶上显现蛋白质样品的分离模式; 和（d）基于蛋白质的可视化分离模式来确定小麦粉中的原始小麦的品种。版权所有（C）2005，JPO＆NCIPI

5. 发明专利

JP2006034252A Oryza sativa resistant to composite environmental stress 审中-公开
标题翻译： ORYZA SATIVA抗复合环境应力
公开(公告)号：JP2006034252A
公开(公告)日：2006-02-09
申请号：JP2004222758
申请日：2004-07-30
申请人： Hokkaido Green Bio Kenkyusho:Kk , National Agriculture & Bio-Oriented Research Organization , 株式会社北海道グリーンバイオ研究所 , 独立行政法人農業・生物系特定産業技術研究機構
发明人： SATO YUTAKA , TANIDA MASATOSHI , SARUYAMA HARUO
IPC分类号： A01H5/00 , A01G7/00 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a plant cultured under a composite environmental stress.
SOLUTION: A transgenic gramineous plant has resistance to composite environmental stress, into which at least one of following genes (a)-(d) is transduced. (a) A gene comprising a DNA including a specific base sequence. (b) A gene comprising a DNA encoding a protein having high temperature resistance imparting activity and hybridizing with a DNA with a specific base sequence under a stringent condition. (c) A gene encoding a protein including a specific amino acid sequence. (d) A gene encoding a protein having high temperature resistance imparting activity and comprising an amino acid sequence having one or more amino acids deleted, substituted or added to a specific amino acid sequence.
COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：待解决的问题：提供在复合环境应力下培养的植物。解决方案：转基因禾本科植物对复合环境胁迫具有抗性，其中至少一个以下基因（a） - （d）被转导到其中。（a）包含特定碱基序列的DNA的基因。（b）包含编码具有耐高温性的蛋白质的DNA并且在严格条件下与特定碱基序列的DNA杂交的基因。（c）编码包含特定氨基酸序列的蛋白质的基因。（d）编码具有耐高温性的蛋白质的基因，其具有缺失，取代或添加到特定氨基酸序列中的一个或多个氨基酸的氨基酸序列。版权所有（C）2006，JPO＆NCIPI

6. 发明专利

JP2005117990A Method for creating superstrong wheat line 审中-公开
标题翻译：创建超级牛排的方法
公开(公告)号：JP2005117990A
公开(公告)日：2005-05-12
申请号：JP2003358310
申请日：2003-10-17
申请人： Hokkaido Green Bio Kenkyusho:Kk , National Agriculture & Bio-Oriented Research Organization , 株式会社北海道グリーンバイオ研究所 , 独立行政法人農業・生物系特定産業技術研究機構
发明人： FUNATSUKI WAKAKO , TAKADA KANENORI , YAMAUCHI HIROAKI , TABIKI TADASHI , NISHIO ZENTA , SARUYAMA HARUO , HACHIMAN ERIKO
IPC分类号： A01H5/00
摘要： PROBLEM TO BE SOLVED: To efficiently create a superstrong wheat line.
SOLUTION: The method for creating the superstrong wheat line comprises (a) a step for collecting wheat seeds from a wheat variety having at least either one of 5+10 high-molecular weight glutenin subunit and 42kD low-molecular weight glutenin or a wheat line using a mating parent as the line, (b) a step for separating the high-molecular weight glutenin of wheat seed protein with sodium dodecylsulfate (SDS)-polyacrylamide gel electrophoresis and separating the low-molecular weight glutenin with two-dimensional electrophoresis, (c) a step for selecting a wheat line obtained by collecting wheat seed in which at least either one of 5+10 high-molecular weight glutenin subunit or 42 kD low-molecular weight glutenin is recognized in a separation pattern of gluten on each gel and (d) a step for obtaining the superstrong wheat line in the posterity by self-propagating the selected wheat line or mating the selected wheat line with other wheat variety or line.
COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：有效地建立超强的小麦生产线。解决方案：用于制造超强度小麦品系的方法包括（a）从具有5 + 10个高分子量麦谷蛋白亚基和42kD低分子量麦谷蛋白中的至少任一种的小麦品种收集小麦种子的步骤，或使用交配母体作为生产线的小麦品系，（b）用十二烷基硫酸钠（SDS） - 聚丙烯酰胺凝胶电泳分离小麦种子蛋白的高分子量麦谷蛋白的步骤，并用二维分离低分子量麦谷蛋白电泳，（c）选择通过收集小麦种子获得的小麦品系的步骤，其中5 + 10个高分子量麦谷蛋白亚基或42kD低分子量麦谷蛋白中的至少一个以麸质的分离模式被识别每个凝胶和（d）通过自选繁殖选择的小麦品系或将选定的小麦品系与其他小麦品种或品系相交的步骤，在后代获得超强的小麦品系。版权所有（C）2005，JPO＆NCIPI

7. 发明专利

JP2003319782A Method for identifying cultivar of rice plant 审中-公开
标题翻译：用于鉴定稻米植物的方法
公开(公告)号：JP2003319782A
公开(公告)日：2003-11-11
申请号：JP2002130645
申请日：2002-05-02
申请人： Hokkaido Green Bio Kenkyusho:Kk , Hokuren Federation Of Agricult Coop:The , ホクレン農業協同組合連合会 , 株式会社北海道グリーンバイオ研究所
发明人： FUJINO KENJI
IPC分类号： C12N15/09 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To provide a DNA marker having an excellent ability to identify the cultivar of rice plants and further an identifying means for the cultivar of the rice plants using the DNA marker. SOLUTION: The method for identifying the cultivar of the rice plants comprises identifying the cultivar of the rice plants using a specific simple sequence repeats (SSR) sequence of a gene of the rice plants as in index. The primer for amplifying the gene suitably usable for the method for identifying the cultivar is provided. Thereby, the plurality of cultivars of the rice plants can be identified. COPYRIGHT: (C)2004,JPO
摘要翻译：要解决的问题：提供具有优异的鉴定水稻品种的能力的DNA标记物，并且使用DNA标记物进一步鉴定水稻品种的鉴定方法。解决方案：用于鉴定水稻植物品种的方法包括使用水稻植物基因的特异性简单序列重复序列（SSR）序列来鉴定水稻植物的品种。提供了用于扩增适用于鉴定栽培品种的方法的基因的引物。因此，可以鉴定水稻植物的多个品种。版权所有（C）2004，JPO

8. 发明专利

JP2005341937A Oral vaccine carrier system 有权
标题翻译：口腔疫苗载体系统
公开(公告)号：JP2005341937A
公开(公告)日：2005-12-15
申请号：JP2004169259
申请日：2004-06-07
申请人： Frontier Science Co Ltd , Hokkaido Green Bio Kenkyusho:Kk , Kitasato Inst:The , National Institute Of Advanced Industrial & Technology , 株式会社フロンティア・サイエンス , 株式会社北海道グリーンバイオ研究所 , 独立行政法人産業技術総合研究所 , 社団法人北里研究所
发明人： MATSUMURA TAKESHI , ITO AKIRA , KOBAYASHI EIKI , KUME KATSUMI , ITO KEIZO , ITSUCHIYOUDA NORIKO , SUGIMOTO CHIHIRO
IPC分类号： A01H5/00 , A61K36/00 , A61K36/81 , A61K38/00 , A61K39/00 , A61P37/00 , C12N15/09 , C12N15/82 , A61K35/78
CPC分类号： A61K36/81 , A61K38/00 , A61K2039/517 , A61K2039/542 , C12N15/8258 , C12N2770/16022
摘要： PROBLEM TO BE SOLVED: To obtain a system for exhibiting the function of a peptide by oral administration of a recombinant plant body expressing an objective peptide and a gene recombination plant. SOLUTION: The carrier means for oral administration of the objective peptide comprises oral administration of a plant body (potato) expressing a gene by fusion between LRE1 gene encoding the objective gene such as immunogenic peptide LRE1 derived from a second schizont of Leucocytozoon caulleryi and Norwalk virus envelope gene. The gene recombination plant is obtained. The orally administered objective peptide reaches an objective location (intestinal mucosa, etc.) without being hydrolyzed in the digestive tract to induce the function of the objective peptide. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：待解决的问题：通过口服施用表达目标肽的重组植物体和基因重组植物来获得表现出肽功能的系统。口服给药目的肽的载体包括口服施用表达基因的植物体（马铃薯），所述基因通过编码目标基因的LRE1基因（例如衍生自白细胞介素的第二裂殖体的免疫原性LRE1）和诺瓦克病毒包膜基因。获得基因重组。口服给药的目的肽达到客观位置（肠粘膜等），而不在消化道中水解以诱导目的肽的功能。版权所有（C）2006，JPO＆NCIPI

9. 发明专利

JP2003009692A Stress-resistant plant 有权
标题翻译：耐应力植物
公开(公告)号：JP2003009692A
公开(公告)日：2003-01-14
申请号：JP2001199272
申请日：2001-06-29
申请人： Hokkaido Green Bio Kenkyusho:Kk , Hokuren Federation Of Agricult Coop:The , National Agricultural Research Organization , ホクレン農業協同組合連合会 , 株式会社北海道グリーンバイオ研究所 , 独立行政法人農業技術研究機構
发明人： SATO YUTAKA , TANIDA MASATOSHI , SARUYAMA HARUO
IPC分类号： A01H5/00 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide stress-resistant transformed plant. SOLUTION: The stress-resistant plant includes a recombinant vector bearing the gene encoding the following protein (a) or (b) as well as the overexpression promoter: (a) the protein having the amino acid sequence represented by the sequence No.2 (refer to the patent specification); (b) the protein having the amino acid sequence in which one or several amino acids are deleted from, substituted with other amino acids or added thereto and has the ascorbate peroxidase activity.
摘要翻译：要解决的问题：提供耐应力转化植物。解决方案：抗应激植物包括具有编码以下蛋白质（a）或（b）的基因的重组载体以及过表达启动子：（a）具有由序列号2表示的氨基酸序列的蛋白质参照专利说明书）; （b）具有其中一个或几个氨基酸缺失，被其它氨基酸取代或加入其中并具有抗坏血酸过氧化物酶活性的氨基酸序列的蛋白质。

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