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1. 发明申请

WO2022234009A2 METHODS FOR STIMULATING AND TRANSDUCING T CELLS 审中-公开
公开(公告)号：WO2022234009A2
公开(公告)日：2022-11-10
申请号：PCT/EP2022/062139
申请日：2022-05-05
申请人： JUNO THERAPEUTICS GMBH
发明人： POLTORAK, Mateusz Pawel , FRAESSLE, Simon , EFFENBERGER, Manuel
IPC分类号： C07K14/725 , A61K39/00 , C12N5/0783 , C12N15/86 , G01N30/02 , A61K2039/5156 , A61K2039/804 , A61K39/001112 , A61K39/39 , C07K14/54 , C07K14/7051 , C07K16/2809 , C07K16/2818 , C07K2317/55 , C12N2510/00 , C12N2740/16043 , C12N5/0636
摘要： Provided herein are methods for selecting, stimulating, and transducing cells in a sample using column chromatography, and collecting and/or eluting the cells from the column without using additional steps or reagents to facilitate detachment of the cells from the column. In some aspects, the methods provided herein reduce the time needed to generate a population of selected, stimulated, and transduced cells useful for, ultimately, cell therapy, compared to existing methods. Also provided are articles of manufacture and apparatus thereof.

2. 发明申请

WO2022207848A1 IMAGING SYSTEM AND METHOD OF OPERATION 审中-公开
公开(公告)号：WO2022207848A1
公开(公告)日：2022-10-06
申请号：PCT/EP2022/058672
申请日：2022-03-31
申请人： MICROGRAPHIA BIO LIMITED
发明人： THOMPSON, Christopher C.
IPC分类号： G01N21/64 , G02B21/36 , G01N2021/6439 , G01N21/6428 , G01N21/6458 , G01N21/6486 , G02B21/367
摘要： An imaging system and a method of operating an imaging system for imaging a biological target is provided. The imaging system comprises a fluorescence microscope, a light source, an imaging device, a processor, a probe reagent, and a chemical buffer. The method comprises: acquiring, by the imaging device and through the fluorescence microscope, a first image of the biological target at a first time; determining, by the processor, a first value of an image acquisition metric of the first image; determining, by the processor, a first difference between the first value of the image acquisition metric and a predetermined value of the image acquisition metric; adjusting, by the processor, one or more physical parameters of the imaging system based on the first difference, wherein the one or more physical parameters of the imaging system comprise one or more of: an illumination characteristic of the light source, concentration of the probe reagent, and concentration of the chemical buffer; and repeating the steps for a second image acquired at a second time subsequent to the first time.

3. 发明申请

WO2022175483A1 METHOD AND KITS FOR MULTIPLEXED FLUORESCENT MICROSCOPY 审中-公开
公开(公告)号：WO2022175483A1
公开(公告)日：2022-08-25
申请号：PCT/EP2022/054140
申请日：2022-02-18
申请人： MICROGRAPHIA BIO LIMITED
发明人： ADJALLEY, Sophie , THOMPSON, Christopher C.
IPC分类号： C12Q1/6804
摘要： Provided is a method for multiplexed fluorescence microscopy comprising contacting a fixed sample with a set of binding agent-T-oligonucleotide conjugates to allow the binding agents to bind to any binding partners present in the sample, wherein the set comprises a plurality of binding agents having different specificities and the sequence of the T-oligonucleotide is unique to the binding agent to which it is conjugated, contacting the sample and any bound binding agents resulting from step a with a FRET- oligonucleotide, illuminating the sample with a wavelength to cause excitation of the FRET- oligonucleotide's emitter molecule, and observing the fluorescent kinetic profile of the sample at the FRET-oligonucleotide emitter molecule's emission wavelength at one or more pixels over time, wherein the FRET-oligonucleotide can hybridise to multiple T-oligonucleotides in the set, to form multiple pairs, and wherein the dissociation and reassociation between each different pair generates a fluorescent kinetic profile that is unique within that set to that pair. Also provided are associated kits, sets of binding agent T-oligonucleotide conjugates and corresponding FRET-oligonucleotides, and methods of designing such sets.

4. 发明授权

EP1438427B1 NUCLEIC ACID MARKERS FOR USE IN DETERMINING PREDISPOSITION TO NEOPLASM AND/OR ADENOMA 有权
标题翻译： FOR USE NUCLEIC MARKER确定易患肿瘤和/或腺瘤
公开(公告)号：EP1438427B1
公开(公告)日：2016-04-20
申请号：EP02757979.6
申请日：2002-09-13
申请人： Clinical Genomics Pty. Ltd
发明人： JAMES, Robert , HENRY, Julianne , KAZENWADEL, Jan , VAN HOST PELLEKAAN, Nick , MACPHERSON, Anne , O'CONNOR, Susan
IPC分类号： C12Q1/68 , C12N15/11 , C07H21/04 , C07K14/435 , C07K14/47
CPC分类号： C07K14/47 , C12Q1/6886 , C12Q2600/158

5. 发明授权

EP2467716B1 IDENTIFICATION AND QUANTIFICATION OF BIOMARKERS FOR EVALUATING THE RISK OF PRETERM BIRTH 有权
标题翻译：鉴定和生物标志物的定量评价一个诞生初期的风险
公开(公告)号：EP2467716B1
公开(公告)日：2015-01-28
申请号：EP10810580.0
申请日：2010-08-19
申请人： University of Utah Research Foundation , Brigham Young University , IHC Health Services
发明人： GRAVES, Steven, W. , ESPLIN, Michael Sean
IPC分类号： G01N33/68
CPC分类号： G01N33/689 , G01N2800/368

6. 发明公开

EP2742118A1 IMPROVED AQUACULTURE FEED COMPOSITIONS 审中-公开
标题翻译：改进的水产饲料组合物
公开(公告)号：EP2742118A1
公开(公告)日：2014-06-18
申请号：EP12815607.2
申请日：2012-02-10
申请人： E. I. Du Pont de Nemours and Company
发明人： ODOM, James, M. , AVGOUSTI, Marios , BELL, Timothy, Allan , GUTSCHE, Oliver , HUMPHREY, John, L. , HUTCHENSON, Keith, W. , ORLANDI, Robert, D.
IPC分类号： C11B1/10
CPC分类号： A23K50/80 , A23K10/22 , A23K20/158 , A23K20/179 , A23K40/20 , A23K40/25 , Y02A40/818
摘要： Aquaculture feed compositions having a ratio of concentration of omega-3 polyunsaturated fatty acids of eicosapentaenoic acid [“EPA”; cis-5,8,11,14,17-eicosapentaenoic acid; 20:5] to concentration of docosahexaenoic acid [“DHA”; cis-4,7,10,13,16,19-docosahexaenoic acid; 22:6] greater than 2:1 based on the individual concentrations of EPA and DHA in the aquaculture feed composition, and having a biomass source of carotenoid(s), are disclosed. Furthermore, such aquaculture feed compositions may have a concentration of the sum of EPA and DHA that is at least about 0.8%, measured as a weight percent of the aquaculture feed composition.

7. 发明授权

EP2216413B1 Method of pretreating sample for measurement of glycated amine and method of measuring glycated amine 有权
标题翻译：一种用于预处理用于糖化胺和方法的测定用试样用于测量糖化胺的方法
公开(公告)号：EP2216413B1
公开(公告)日：2013-07-10
申请号：EP10005386.7
申请日：2002-10-09
申请人： ARKRAY, Inc.
发明人： Yonehara, Satoshi , Komori, Tsuguki
IPC分类号： C12Q1/26 , C12Q1/37 , C12Q1/28 , G01N33/48 , G01N33/50 , G01N33/72
CPC分类号： C12Q1/26 , C12Q1/37 , G01N33/723 , G01N2333/906

8. 发明公开

EP2560473A1 GENE SWITCH COMPOSITIONS AND METHODS OF USE 审中-公开
标题翻译：根施年龄构成及使用方法
公开(公告)号：EP2560473A1
公开(公告)日：2013-02-27
申请号：EP11719688.1
申请日：2011-04-14
申请人： Pioneer Hi-Bred International Inc. , E.I. Du Pont De Nemours And Company
发明人： FANG, Jin , GORDON-KAMM, William, J. , LOWE, Keith, S. , MCBRIDE, Kevin, E. , MCGONIGLE, Brian , SIMMONS, Carl
IPC分类号： A01H5/00 , C12N15/82
CPC分类号： C12N15/8217
摘要： Compositions and methods relating to the use of sulfonylurea-mediated control of gene expression are provided. Compositions include sulfonylurea responsive chemical switches wherein the gene expression is regulated by a sulfonylurea compound. Compositions also include polynucleotides encoding the polypeptides as well as constructs, vectors, prokaryotic and eukaryotic cells, and eukaryotic organisms including plants and seeds comprising the polynucleotide, and/or produced by the methods. Also provided are methods to regulate expression of a polynucleotide of interest in a cell or organism, and methods to modify a genome, including in a plant or plant cell.

9. 发明公开

EP2559442A1 METHOD FOR REGULATING MICRORNA CONTENT IN ORGANISMS AND USES THEREOF 有权
标题翻译：方法用于调节微小RNA含量的生物和需求
公开(公告)号：EP2559442A1
公开(公告)日：2013-02-20
申请号：EP10849635.7
申请日：2010-04-13
申请人： Jiangsu Mingma Biotech Co., Ltd
发明人： ZENG, Ke , ZHANG, Chenyu , ZHANG, Junfeng , LI, Haijin
IPC分类号： A61K48/00 , C12N15/113 , C12N5/10 , C12N15/88 , A61P31/16 , A61P31/12 , A61P31/18 , A61P31/04 , A61P31/06 , A61P31/00 , A61P37/00 , A61P7/00 , A61P9/00 , A61P9/10 , A61P5/00 , A61P1/00 , A61P25/00 , A61P13/00 , A61P15/00 , A61P19/00
CPC分类号： A61K35/14 , A61K35/28 , A61K35/36 , C12N15/111 , C12N15/88 , C12N2310/141 , C12N2320/32
摘要： The present invention relates to a method for modulating miRNA content in living beings and the use thereof, whereof the miRNAs are delivered initiatively and selectively by microparticles/MVs/exosomes to the circulatory system and target cells/tissues to perform various functions. The invention provides a novel combination of molecules that mediates the inter-cellular communication: the miRNAs secreted by cells through cellular MVs. Meanwhile, the present invention also provides a method for preparing cellular MVs entrapping certain miRNAs, and, according to the regulation and modification of gene expression in cells and tissues with the miRNA-entrapping cellular MVs, provides a novel method for modulating miRNA content in living beings, which is effective and adoptable to all cell types.

10. 发明公开

EP2474614A9 DNA FRAGMENT FOR IMPROVING TRANSLATION EFFICIENCY, AND RECOMBINANT VECTOR CONTAINING SAME 审中-公开
标题翻译：提高翻译效率的DNA片段和含有相同片段的重组载体
公开(公告)号：EP2474614A9
公开(公告)日：2013-01-16
申请号：EP10812255.7
申请日：2010-08-25
申请人： Helix, Co., Limited
发明人： NA, Yun Jeong , JEON, Eun Hyun , KWON, Eun Hye , KIM, Yong Woo , SOHN, Eun Ju , HWANG, In Hwan
IPC分类号： C12N15/11 , C12N15/63 , C12N15/66
CPC分类号： C12N15/67
摘要： The present invention relates to a DNA fragment for improving translation efficiency, and a recombinant vector containing the same, and more specifically, to a DNA fragment which comprises any one nucleotide sequence selected from the group consisting of SEQ ID NOs: 1-6, SEQ ID NOs: 8-10, SEQ ID NOs: 13 and 14 and SEQ ID NO: 16, and improves the translation efficiency of a heterologous protein placed in the downstream, and a recombinant vector containing the DNA fragment. The DNA fragment for improving translation efficiency according to the present invention and a recombinant vector containing the same can improve the translation of a heterologous protein in a transgenic plant. In addition, if a leader polynucleotide inducing the targeting to a particular cellular organelle of a plant is further linked to the recombinant vector in an operable manner, the heterologous protein is targeted to the specific cellular organelle and can be stably accumulated, thereby enabling the mass production of the heterologous protein from the plant.
摘要翻译：本发明涉及用于提高翻译效率的DNA片段和含有其的重组载体，并且更具体地涉及包含选自SEQ ID NO：1-6，SEQ SEQ ID NO：13和14以及SEQ ID NO：16，并且提高了置于下游的异源蛋白质的翻译效率，以及含有该DNA片段的重组载体。根据本发明的用于提高翻译效率的DNA片段和包含其的重组载体可以改善转基因植物中异源蛋白质的翻译。另外，如果以可操作的方式将诱导针对特定细胞器的靶向的前导序列多核苷酸以可操作的方式进一步连接至重组载体，则异源蛋白质被靶向特定细胞器并且可以稳定地累积，从而使质量从植物中产生异源蛋白质。

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