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1. 发明公开

EP2474614A9 DNA FRAGMENT FOR IMPROVING TRANSLATION EFFICIENCY, AND RECOMBINANT VECTOR CONTAINING SAME 审中-公开
标题翻译：提高翻译效率的DNA片段和含有相同片段的重组载体
公开(公告)号：EP2474614A9
公开(公告)日：2013-01-16
申请号：EP10812255.7
申请日：2010-08-25
申请人： Helix, Co., Limited
发明人： NA, Yun Jeong , JEON, Eun Hyun , KWON, Eun Hye , KIM, Yong Woo , SOHN, Eun Ju , HWANG, In Hwan
IPC分类号： C12N15/11 , C12N15/63 , C12N15/66
CPC分类号： C12N15/67
摘要： The present invention relates to a DNA fragment for improving translation efficiency, and a recombinant vector containing the same, and more specifically, to a DNA fragment which comprises any one nucleotide sequence selected from the group consisting of SEQ ID NOs: 1-6, SEQ ID NOs: 8-10, SEQ ID NOs: 13 and 14 and SEQ ID NO: 16, and improves the translation efficiency of a heterologous protein placed in the downstream, and a recombinant vector containing the DNA fragment. The DNA fragment for improving translation efficiency according to the present invention and a recombinant vector containing the same can improve the translation of a heterologous protein in a transgenic plant. In addition, if a leader polynucleotide inducing the targeting to a particular cellular organelle of a plant is further linked to the recombinant vector in an operable manner, the heterologous protein is targeted to the specific cellular organelle and can be stably accumulated, thereby enabling the mass production of the heterologous protein from the plant.
摘要翻译：本发明涉及用于提高翻译效率的DNA片段和含有其的重组载体，并且更具体地涉及包含选自SEQ ID NO：1-6，SEQ SEQ ID NO：13和14以及SEQ ID NO：16，并且提高了置于下游的异源蛋白质的翻译效率，以及含有该DNA片段的重组载体。根据本发明的用于提高翻译效率的DNA片段和包含其的重组载体可以改善转基因植物中异源蛋白质的翻译。另外，如果以可操作的方式将诱导针对特定细胞器的靶向的前导序列多核苷酸以可操作的方式进一步连接至重组载体，则异源蛋白质被靶向特定细胞器并且可以稳定地累积，从而使质量从植物中产生异源蛋白质。

2. 发明公开

EP2486791A2 HIGHLY PATHOGENIC AVIAN INFLUENZA VIRUS PROTEIN VACCINE DERIVED FROM TRANSGENIC PLANTS, AND METHOD FOR PREPARING SAME 审中-公开
标题翻译：关转基因植物中获得的蛋白疫苗高致病性禽流感病毒及其制造方法
公开(公告)号：EP2486791A2
公开(公告)日：2012-08-15
申请号：EP10822229.0
申请日：2010-10-06
申请人： Helix, Co., Limited
发明人： HWANG, In Hwan , SOHN, Eun Ju , KWON, Eun Hye , NA, Yun Jeong , JEON, Eun Hyun , IM, Se Jin , PARK, Ki Seok , SUNG, Young Chul
IPC分类号： A01H1/00 , C12N15/40 , C12N15/82 , A61K39/12
CPC分类号： A61K39/145 , A61K39/12 , C12N15/8258 , C12N2760/16134
摘要： The present invention relates to a method for producing transgenic plants, which involves producing hemagglutinin proteins of the H5N1 virus in a highly efficient manner using a plant transformation recombinant vector, wherein said vector can express, in a highly efficient manner, hemagglutinin proteins of the H5N1 virus which is the highly pathogenic avian influenza virus, and can transport proteins expressed in plants to the endoplasmic reticulum and enable the retention of the proteins in the endoplasmic reticulum so as to accomplish glycosylation which is necessarily required for antigen activity. The present invention also relates to a method for the mass production of hemagglutinin proteins of the antigenic H5N1 virus from the transgenic plants, and to a vaccine composition for the avian influenza virus comprising the transgenic plants or the hemagglutinin proteins produced from the plants. The method for producing hemagglutinin proteins of the H5N1 virus using transgenic plants according to the present invention enables the mass production of hemagglutinin proteins at a low cost. The plant transformation recombinant vector used in the present invention has a cellulose-binding domain inserted therein to separate proteins expressed in plants in a quick and easy manner. In addition, transgenic plants according to the present invention can be taken in the form of feed additives or the like, and therefore can be used as edible vaccines. Further, antigenic hemagglutinin proteins produced by the method induce immune response when administered to an animal model, and therefore can be used not only as protein vaccines for the H5N1 avian influenza virus, but also as diagnostic reagents for avian influenza virus infection.
摘要翻译：本发明涉及一种用于产生转基因植物，其涉及使用植物转化的重组载体制备H5N1病毒的血凝素蛋白以高度有效的方式，worin所述载体可以表达，以高度有效的方式，H5N1的血凝素蛋白所有这一切是病毒高致病性禽流感病毒，并能运送植物过度到内质网的蛋白质，使蛋白质的保留的内质网，以实现糖基化所有这一切都必然是必需的抗原活性。因此，本发明涉及用于大规模生产来自转基因植物的H5N1病毒抗原的血凝素蛋白的方法，并为禽流感病毒，其包含在转基因植物或从植物产生的血凝素蛋白的疫苗组合物。用于使用转基因植物gemäß到本发明的H5N1病毒的血凝素蛋白的方法能够大量生产血凝素蛋白的低成本。在本发明中使用的植物转化的重组载体具有插入其中以快速且容易的方式在植物中过表达的蛋白质分开的纤维素结合结构域。此外，转基因植物gemäß到本发明可以采取在饲料添加剂或类似物的形式，并且因此可被用作食用的疫苗。此外，由该方法制备的抗原血凝素蛋白诱导的免疫应答在施用给动物模型，并因此可以不仅用作蛋白疫苗对H5N1禽流感病毒，所以但对于禽流感病毒感染的诊断试剂。

3. 发明公开

EP2474614A2 DNA FRAGMENT FOR IMPROVING TRANSLATION EFFICIENCY, AND RECOMBINANT VECTOR CONTAINING SAME 审中-公开
标题翻译： DNA-FRAGMENT ZUR VERBESSERUNG DER TRANSLATIONSEFFIZIENZ UND REKOMBINANTER VEKTOR DAMIT
公开(公告)号：EP2474614A2
公开(公告)日：2012-07-11
申请号：EP10812255.7
申请日：2010-08-25
申请人： Helix, Co., Limited
发明人： NA, Yun Jeong , JEON, Eun Hyun , KWON, Eun Hye , KIM, Yong Woo , SOHN, Eun Ju , HWANG, In Hwan
IPC分类号： C12N15/11 , C12N15/63 , C12N15/66
CPC分类号： C12N15/67
摘要： The present invention relates to a DNA fragment for improving translation efficiency, and a recombinant vector containing the same, and more specifically, to a DNA fragment which comprises any one nucleotide sequence selected from the group consisting of SEQ ID NOs: 1-6, SEQ ID NOs: 8-10, SEQ ID NOs: 13 and 14 and SEQ ID NO: 16, and improves the translation efficiency of a heterologous protein placed in the downstream, and a recombinant vector containing the DNA fragment. The DNA fragment for improving translation efficiency according to the present invention and a recombinant vector containing the same can improve the translation of a heterologous protein in a transgenic plant. In addition, if a leader polynucleotide inducing the targeting to a particular cellular organelle of a plant is further linked to the recombinant vector in an operable manner, the heterologous protein is targeted to the specific cellular organelle and can be stably accumulated, thereby enabling the mass production of the heterologous protein from the plant.
摘要翻译：本发明涉及用于提高翻译效率的DNA片段和含有该DNA片段的重组载体，更具体地说，涉及包含选自SEQ ID NO：1-6，SEQ ID NO：1-6中任一个核苷酸序列的DNA片段 ID NOs：8-10，SEQ ID NO：13和14以及SEQ ID NO：16，并提高了放置在下游的异源蛋白质的翻译效率和含有该DNA片段的重组载体。用于提高根据本发明的翻译效率的DNA片段和含有该DNA片段的重组载体可以改善转基因植物中异源蛋白质的翻译。此外，如果诱导针对植物的特定细胞器的靶向多核苷酸多核苷酸进一步以可操作的方式与重组载体连接，则异源蛋白质靶向特异性细胞器官并可稳定地积聚，从而使质量从植物中产生异源蛋白质。

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