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81. 发明授权

US5597688A Cell fixative and method of analyzing virally infected cells 失效
标题翻译：细胞固定剂和分析病毒感染细胞的方法
公开(公告)号：US5597688A
公开(公告)日：1997-01-28
申请号：US467799
申请日：1995-06-06
申请人： Mark C. Connelly , Utpal R. Chakraborty , Houston G. Brooks, Jr.
发明人： Mark C. Connelly , Utpal R. Chakraborty , Houston G. Brooks, Jr.
IPC分类号： C12Q1/00 , G01N1/30 , G01N15/14 , G01N33/48 , G01N33/533 , G01N33/545 , G01N33/569 , C12Q1/70 , G01N33/53
CPC分类号： G01N33/56988 , G01N1/30 , G01N33/56972 , G01N33/56983 , G01N2001/305 , Y10S435/975 , Y10S436/805 , Y10T436/10 , Y10T436/101666 , Y10T436/108331 , Y10T436/25
摘要： The present invention provides a novel cellular fixative composition and method of cellular fixing and cellular analysis wherein cells may be fixed for further analysis without destroying the cell's surface markers, cellular morphology, and the cell's light scattering properties. Treatment of cells with the fixative as described herein also allows antibodies or .other desired components to enter the cell through the cellular membrane, without allowing important contents of the cell to escape. As an added benefit, the fixative composition of the invention may also kill or at least inactivate or reduce the activity of deadly virus contained in the cell. In its broadest aspect, the presently claimed fixative composition comprises at least three components, and in more preferred embodiments, four components. In a particularly preferred embodiment, the fixative composition described herein comprises at least one compound suitable for increasing the permeability of a cellular membrane, at least one compound that facilitates transportation of components across cellular membranes, at least one detergent, and at least one compound having the structure:R.sub.1 R.sub.2 R.sub.3 R.sub.4 R.sub.5 --Ar--X
摘要翻译：本发明提供了一种新的细胞固定组合物和细胞固定和细胞分析方法，其中细胞可以固定用于进一步分析，而不破坏细胞的表面标志物，细胞形态和细胞的光散射性质。用本文所述的固定剂处理细胞还允许抗体或其它所需组分通过细胞膜进入细胞，而不允许细胞的重要内容物逸出。作为额外的益处，本发明的固定组合物还可以杀死或至少灭活或降低细胞中包含的致命病毒的活性。在其最广泛的方面，目前要求的固定剂组合物包含至少三种组分，在更优选的实施方案中包含四种组分。在一个特别优选的实施方案中，本文描述的固定剂组合物包含至少一种适合于增加细胞膜的渗透性的化合物，至少一种促进组分跨细胞膜运输的化合物，至少一种洗涤剂和至少一种具有结构：R1R2R3R4R5-Ar-X

82. 发明授权

US5594808A Method and system for classifying agglutination reactions 失效
标题翻译：用于分类凝集反应的方法和系统
公开(公告)号：US5594808A
公开(公告)日：1997-01-14
申请号：US163996
申请日：1993-12-08
申请人： Jian Shen , Mykola Yaremko , Rosemary Chachowski , Josef Atzler , Thierry Dupinet , Daniel Kittrich , Hansjoerg Kunz , Karl Puchegger , Reiner Rohlfs
发明人： Jian Shen , Mykola Yaremko , Rosemary Chachowski , Josef Atzler , Thierry Dupinet , Daniel Kittrich , Hansjoerg Kunz , Karl Puchegger , Reiner Rohlfs
IPC分类号： G01N21/82 , G06K9/00
CPC分类号： G01N33/4905 , G01N21/82
摘要： A method and system for analyzing a solution for an agglutination pattern. The method comprises the steps of producing an illustrated image of the solution on an array of pixels, and assigning to each pixel in the illuminated image, a data value representing the intensity of the illuminated image on the pixel. Those data values are then processed according to a predetermined program to determine if an agglutination pattern is present and, if so, to classify that pattern into one of a plurality of predefined classes. With the preferred processing procedure, the pixel array is separated into a plurality of zones, and the data values for the pixels in each zone are processed according to a respective predetermined procedure to determine values for a predefined set of variables. Then, those determined values are processed to determine whether an agglutination pattern is present in the solution, and if so, to classify that pattern into one of the predefined classes.
摘要翻译：用于分析凝集图案的方案的方法和系统。该方法包括以下步骤：在像素阵列上产生解决方案的所示图像，并且向照射图像中的每个像素分配表示像素上的照射图像的强度的数据值。然后根据预定程序处理那些数据值，以确定是否存在凝集模式，如果是，则将该模式分类为多个预定类别之一。利用优选的处理过程，将像素阵列分成多个区域，并且根据相应的预定过程处理每个区域中的像素的数据值，以确定预定义变量集合的值。然后，处理这些确定的值以确定解决方案中是否存在凝集模式，如果是，则将该模式分类为预定义类之一。

83. 发明授权

US5578269A Automated blood analysis system with an integral centrifuge 失效
标题翻译：具有一体式离心机的自动血液分析系统
公开(公告)号：US5578269A
公开(公告)日：1996-11-26
申请号：US163997
申请日：1993-12-08
申请人： Mykola Yaremko , Rosemary Chachowski , Marcel Frischknecht , Gregor Batliner , Linus Flueler , Marco Forster , Martin Gander , Beat Gretener , Walter Imfeld , Hansjoerg Kunz , Martin Kuster , Karl Puchegger
发明人： Mykola Yaremko , Rosemary Chachowski , Marcel Frischknecht , Gregor Batliner , Linus Flueler , Marco Forster , Martin Gander , Beat Gretener , Walter Imfeld , Hansjoerg Kunz , Martin Kuster , Karl Puchegger
IPC分类号： G01N35/00 , G01N35/02 , G01N35/04 , G01N35/10 , G01N9/30
CPC分类号： G01N35/025 , G01N2035/00495 , G01N2035/00752 , G01N2035/0449 , G01N2035/0455 , G01N2035/0465 , G01N2035/1025 , G01N35/1079 , Y10T436/11 , Y10T436/111666 , Y10T436/113332 , Y10T436/114165 , Y10T436/114998 , Y10T436/25375 , Y10T436/2575
摘要： A blood analysis system or instrument, generally, including an incubator station, a sample and reagent holding station, a pipette assembly, a centrifuge, an analysis station, and a transport assembly. Generally, the incubation station holds containers while reagents and fluids are being dispensed in those containers, and, if desired, for incubating the containers. The sample and reagent holding station holds samples and a plurality of reagents, and the pipette assembly transfers fluids from that sample and reagent holding station to containers in the incubation station. The centrifuge is provided for centrifuging the container, and the analysis station is provided to analyze the containers optically to identify reactions therein. The transport assembly carries the containers between the incubator station, the centrifuge, and the analysis station. Preferably, the pipette assembly is automatically operated to draw fluids and preselected reagents from the sample and reagent holding station, and to dispense fluids into the containers held in the incubation station to produce predetermined solutions therein. Also, the transport subassembly is automatically operated to carry containers from the incubator station to the centrifuge after the predetermined solutions have been produced in the containers, and then to carry the containers from the centrifuge to the analysis station.
摘要翻译：通常，血液分析系统或仪器包括培养箱，样品和试剂保持站，移液管组件，离心机，分析站和运输组件。通常，孵化站容纳容器，同时将试剂和流体分配在这些容器中，并且如果需要，用于孵育容器。样品和试剂保持站保存样品和多个试剂，并且移液管组件将流体从该样品和试剂保持站转移到孵育站中的容器。提供离心机用于离心容器，并提供分析台以光学分析容器以鉴定其中的反应。运输组件携带培养箱，离心机和分析台之间的容器。优选地，移液管组件被自动操作以从样品和试剂保持站抽取流体和预选试剂，并将流体分配到保持在孵化站中的容器中以在其中产生预定的溶液。此外，在容器中产生预定的解决方案之后，运输子组件被自动操作以将容器从培养箱运送到离心机，然后将容器从离心机运送到分析站。

84. 发明授权

US5571726A Kit containing glutaraldehyde coated colloidal metal particles of a preselected size 失效
标题翻译：含有预选大小的含戊二醛涂层胶体金属颗粒的试剂盒
公开(公告)号：US5571726A
公开(公告)日：1996-11-05
申请号：US446533
申请日：1995-05-19
申请人： Houston G. Brooks, Jr. , Chi-Deu Chang , Utpal R. Chakraborty , Henry A. Graham, Jr. , Lloyd L. Hollenbeck, Jr. , Sharon R. Lawler , Jennifer Nasser , Ernest G. Schutt , Albert Venturini
发明人： Houston G. Brooks, Jr. , Chi-Deu Chang , Utpal R. Chakraborty , Henry A. Graham, Jr. , Lloyd L. Hollenbeck, Jr. , Sharon R. Lawler , Jennifer Nasser , Ernest G. Schutt , Albert Venturini
IPC分类号： B01J13/00 , G01N33/543 , G01N33/558 , G01N33/58 , G01N33/74 , G01N33/551 , G01N33/553
CPC分类号： G01N33/587 , B01J13/0004 , B01J13/0043 , G01N33/54326 , G01N33/54386 , G01N33/558 , G01N33/74 , Y10S435/975
摘要： A method and test kit useful in a wide variety of immunoassay systems. A method of producing metal sol reagents containing metal sol particles of a preselected size is provided. A metal containing solution is reduced under optimized pH conditions to produce metal sol particles of a preselected size, the particles are coated with a coupling compound and then bound with at least one selected immunochemically reactive component. Particles having different immunochemical specificities are also mixed to produce reagents having multiple selected immunochemical specificities.
摘要翻译：用于各种免疫测定系统的方法和测试试剂盒。提供一种制备含有预选尺寸的金属溶胶颗粒的金属溶胶试剂的方法。在优化的pH条件下还原含金属溶液以制备预选尺寸的金属溶胶颗粒，用偶联化合物涂覆颗粒，然后与至少一种选择的免疫化学反应性组分结合。具有不同免疫化学特异性的颗粒也混合以产生具有多种选择的免疫化学特异性的试剂。

85. 发明授权

US5422277A Cell fixative composition and method of staining cells without destroying the cell surface 失效
标题翻译：细胞固定组合物和染色细胞的方法，而不破坏细胞表面
公开(公告)号：US5422277A
公开(公告)日：1995-06-06
申请号：US859212
申请日：1992-03-27
申请人： Mark C. Connelly , Utpal R. Chakraborty , Houston G. Brooks, Jr.
发明人： Mark C. Connelly , Utpal R. Chakraborty , Houston G. Brooks, Jr.
IPC分类号： C12Q1/00 , G01N1/30 , G01N15/14 , G01N33/48 , G01N33/533 , G01N33/545 , G01N33/569 , G01N31/00 , C12Q1/02 , G01N1/00
CPC分类号： G01N33/56988 , G01N1/30 , G01N33/56972 , G01N33/56983 , G01N2001/305 , Y10S435/975 , Y10S436/805 , Y10T436/10 , Y10T436/101666 , Y10T436/108331 , Y10T436/25
摘要： A cell fixative composition for fixing the internal components of a cell without disrupting the cell surface components is disclosed. The composition contains a compound for preserving intracellular morphology, a compound for fixing internal components of the cell, a compound which facilitates transportation of components across the cellular membrane, and a compound for increasing permeability of cell membranes. Methods for analyzing cells which have been fixed using the composition are also disclosed.
摘要翻译：公开了用于固定电池的内部组分而不破坏电池表面组分的电池固定组合物。该组合物含有用于保留细胞内形态的化合物，用于固定细胞内部组分的化合物，促进组分跨细胞膜运输的化合物和用于增加细胞膜渗透性的化合物。还公开了使用该组合物固定的细胞分析方法。

86. 发明授权

US5169786A Method of determining levels of extrinsic and intrinsic clotting factors and protein C 失效
标题翻译：确定外在和内在衣物因子和蛋白C水平的方法
公开(公告)号：US5169786A
公开(公告)日：1992-12-08
申请号：US452802
申请日：1989-12-19
申请人： James J. Carroll , Stephen M. Autenrieth
发明人： James J. Carroll , Stephen M. Autenrieth
IPC分类号： G01N33/86
CPC分类号： G01N33/86 , G01N2400/40 , Y10S436/805 , Y10S436/809
摘要： A method of determining levels of extrinsic and intrinsic clotting factors and protein C based upon the reaction rate of the observed clot formation and the first derivative of the reaction rate of observed clot formation is provided. In accordance with the teachings of the invention, the reaction rate of the observed clot formation in a prothrombin time test or an activated partial thromboplastin time assay is determined for both test and normal plasma samples and the reaction rates compared. In another embodiment, the first derivative of the reaction rate of the observed clot formation is determined and the results compared.

87. 外观设计

USD298278S Coagulometer 失效
公开(公告)号：USD298278S
公开(公告)日：1988-10-25
申请号：US803039
申请日：1985-11-27
申请人： Steven R. Savitz , Milan Jorik
设计人： Steven R. Savitz , Milan Jorik

88. 发明授权

US4731326A Disease diagnosis by detection of shed normal tissue antigens 失效
标题翻译：通过检测脱落正常组织抗原的疾病诊断
公开(公告)号：US4731326A
公开(公告)日：1988-03-15
申请号：US616991
申请日：1984-06-04
申请人： Russell E. Thompson , Robert H. Rubin , Nina T. Rubin , Teresa H. Chan
发明人： Russell E. Thompson , Robert H. Rubin , Nina T. Rubin , Teresa H. Chan
IPC分类号： G01N33/574 , G01N33/68 , G01N33/535 , G01N33/545 , G01N33/577
CPC分类号： G01N33/57438 , G01N33/6893 , G01N2800/347 , Y10S436/804 , Y10S436/813
摘要： Methods are provided for the detection of proximal convoluted tubule causing diseases or kidney harmful drug monitoring by detecting the presence of shed normal proximal tubule associated antigens in a body fluid such as urine. The preferred embodiment employs an ELISA sandwich format wherein one monoclonal antibody specific for a first epitopic site on said antigen is immobilized on a solid phase and a second antibody, specific for a second epitopic site on said antigen is directly or indirectly labeled.
摘要翻译：提供了通过检测体液（如尿液）中脱落的正常近端小管相关抗原的存在来检测引起疾病或肾脏有害药物监测的近端回旋小管的方法。优选的实施方案采用ELISA夹心形式，其中对所述抗原上的第一表位位点特异的一种单克隆抗体固定在固相上，并且对所述抗原上的第二表位位点特异性的第二抗体直接或间接标记。

89. 发明授权

US4689398A HTLV test using synthetic peptides 失效
标题翻译：使用合成肽进行HTLV检测
公开(公告)号：US4689398A
公开(公告)日：1987-08-25
申请号：US622430
申请日：1984-06-20
申请人： Ying-Jye Wu , Joseph L. W. Chan
发明人： Ying-Jye Wu , Joseph L. W. Chan
IPC分类号： A61K39/00 , A61K39/21 , A61K39/395 , C07K7/08 , C07K14/15 , C07K16/00 , C07K16/08 , C07K16/10 , C07K17/14 , C07K19/00 , C12N15/02 , C12P21/08 , C12Q1/70 , C12R1/91 , G01N33/53 , G01N33/531 , G01N33/574 , G01N33/577
CPC分类号： C07K14/005 , C07K16/1036 , A61K39/00 , C12N2740/14022 , Y10S930/221
摘要： Synthetic peptides simulating hydrophilic envelope regions and antibodies specific therefor useful for the detection of Human T-cell Leukemia virus or naturally occurring immunoglobulin against HTLV.
摘要翻译：模拟亲水包膜区的合成肽和特异性的抗体可用于检测人类T细胞白血病病毒或针对HTLV的天然存在的免疫球蛋白。

90. 发明授权

US4596464A Screening method for red cell abnormality 失效
标题翻译：红细胞异常筛查方法
公开(公告)号：US4596464A
公开(公告)日：1986-06-24
申请号：US557689
申请日：1983-12-02
申请人： Robert A. Hoffman , Richard L. Kane , Brandon J. Price , Russell J. Gershman
发明人： Robert A. Hoffman , Richard L. Kane , Brandon J. Price , Russell J. Gershman
IPC分类号： G01N15/00 , G01N15/14 , G01N15/02
CPC分类号： G01N15/1456 , G01N2015/0073
摘要： Red cell samples are passed through a zone of focused illumination, and a scatter vs. time pulse is obtained for each. Each pulse is analyzed for pulse width, pulse area, and pulse shape. These parameters are respectively collected, and three coefficients of variation are obtained for each such collection. In addition to these three abnormality factors, the mean of the pulse shape collection constitutes a fourth. The four are respectively subject to statistical criteria, and are combined in predetermined fashion for a single red cell morphology index.
摘要翻译：红细胞样品通过聚焦照明区域，并且获得散射对时间脉冲。分析每个脉冲的脉冲宽度，脉冲面积和脉冲形状。分别收集这些参数，并为每个这样的收集获得三个变异系数。除了这三个异常因素之外，脉冲形状收集的平均值构成第四个。四个分别经受统计学标准，并以预定方式组合为单个红细胞形态学指标。

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