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81. 发明专利

DE10212893A1 未知
公开(公告)号：DE10212893A1
公开(公告)日：2003-10-16
申请号：DE10212893
申请日：2002-03-20
申请人： BASF PLANT SCIENCE GMBH
发明人： BAUER JOERG
IPC分类号： A61K36/064 , A61K36/28 , A61K36/286 , A61K36/31 , A61K36/48 , A61K36/52 , A61K36/63 , A61K36/899 , C12N15/29 , C12N15/82 , C12N15/63 , A01H5/10 , C12N5/14

82. 发明专利

DE10212892A1 未知
公开(公告)号：DE10212892A1
公开(公告)日：2003-10-09
申请号：DE10212892
申请日：2002-03-20
申请人： BASF PLANT SCIENCE GMBH
发明人： KOCK MICHAEL , BAUER JOERG
IPC分类号： A61K38/00 , C12N15/11 , C12N15/29 , C12N15/82 , C12N15/63 , A01H5/00

83. 发明专利

CA2533613C NUCLEIC ACID ENCODING A .DELTA.5-ELONGASE POLYPEPTIDE DERIVED FROM OSTREOCOCCUS TAURI, METHODS AND USES THEREOF 未知
公开(公告)号：CA2533613C
公开(公告)日：2020-03-10
申请号：CA2533613
申请日：2004-07-16
申请人： BASF PLANT SCIENCE GMBH
发明人： ZANK THORSTEN , BAUER JOERG , CIRPUS PETRA , ABBADI AMINE , HEINZ ERNST , QIU XIAO , VRINTEN PATRICIA , SPERLING PETRA , DOMERGUE FREDERIC , MEYER ASTRID , KIRSCH JELENA
IPC分类号： C12N15/54 , A01H5/00 , A23D9/00 , A23K20/158 , A23L33/115 , C11B1/00 , C11B1/02 , C12N5/10 , C12N9/00 , C12N9/02 , C12N9/10 , C12N15/52 , C12N15/53 , C12N15/82 , C12P7/64
摘要： The invention relates to a method for the production of multiply-unsaturated fatty acids in an organism, into which nucleic acids have been introduced, which code for polypeptides with .DELTA. -5 elongase activity. Said nucleic acid sequences, optionally with further nucleic acid sequences, coding for polypeptides for the biosynthesis of fatty acids and lipid metabolism, are advantageously expressed in the organism. Nucleic acid sequences coding for a .DELTA. -6 desaturase, a .DELTA.-5 desaturase, .DELTA. -4 desaturase and/or .DELTA.-6 elongase activity are particularly advantageous and, advantageously, said saturases and elongases are derived from Thalassiosira, Euglena or Ostreococcus. The invention further relates to a method for the production of oils and/or triacylglycerides with an increased content of long-chain, multiply-unsaturated fatty acids. A particular embodiment of the invention is a method for the production of unsaturated co-3 fatty acids and a method for the production of triglycerides with an increased content of unsaturated fatty acids, in particular, of .DELTA.-3 fatty acids with more than three double bonds. Also disclosed is the production of a transgenic organism, preferably a transgenic plant, or a transgenic microorganism with increased content of .omega.-3 fatty acids, oils or lipids with .omega.-3 double bonds as a result of the expression of the elongases and desaturases employed in the above method, preferably in combination with .omega.-3 desaturases, for example a .omega.-3 desaturase from fungi of the family Pythiaceae such as the genus Phytophtora, for example, the genus and species Phytophtora infestans, or a .omega.-3 desaturase from algae such as the family Prasinophyceae, for example, the genus Ostreococcus and, particularly, the genus and species Ostreococcus tauri or diatomacew such as the genus Thalassiosira and, particularly, the genus and species Thalassiosira pseudonana. The invention also relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms containing the nucleic acid sequences and/or the nucleic acid constructs and transgenic organisms containing said nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced according to the above method and use thereof and, furthermore, unsaturated fatty acids and triglycerides with an increased content of unsaturated fatty acids and use thereof.

84. 发明专利

CA2521378C .DELTA.-4 DESATURASES FROM EUGLENA GRACILIS, EXPRESSING PLANTS, AND OILS CONTAINING PUFA 未知
公开(公告)号：CA2521378C
公开(公告)日：2020-01-07
申请号：CA2521378
申请日：2004-04-06
申请人： BASF PLANT SCIENCE GMBH
发明人： CIRPUS PETRA , BAUER JOERG , MEYER ASTRID , HEINZ ERNST , ZAEHRINGER ULRICH
IPC分类号： C12N15/53 , A01H1/00 , A01K67/00 , A01K67/027 , A23D9/00 , C11B1/00 , C12N1/00 , C12N5/10 , C12N9/02 , C12N15/00 , C12N15/63 , C12N15/82 , C12P7/64
摘要： The invention relates to an improved method for the specific production of unsaturated .omega.-3 fatty acids and a method for producing triglycerides containing an increased number of unsaturated fatty acids, especially .omega.-3 fatty acids comprising more than three double bonds. Disclosed is the production of a transgenic organism, preferably a transgenic plant, or a transgenic microorganism containing an increased number of fatty acids, oils, or lipids comprising .DELTA.-4 double bonds as a result of a .DELTA.-4 desaturase being expressed from Euglena gracilis. The invention further relates to expression cassettes containing a nucleic acid sequence, a vector, and organisms containing at least one nucleic acid sequence or an expression cassette. Also disclosed are unsaturated fatty acids and triglycerides having an increased fatty acid content and the use thereof. Fatty acids and triglycerides are used in a plurality of application in the food industry, in animal nutrition, cosmetics, and pharmaceuticals and are suitable for many different applications depending on whether free saturated or unsaturated fatty acids or triglycerides having an increased saturated or unsaturated fatty acid content are used.

85. 发明专利

CA2729496C PROMOTERS FROM BRASSICA NAPUS FOR SEED SPECIFIC GENE EXPRESSION 未知
公开(公告)号：CA2729496C
公开(公告)日：2018-02-13
申请号：CA2729496
申请日：2009-06-30
申请人： BASF PLANT SCIENCE GMBH
发明人： BAUER JOERG , SENGER TORALF
IPC分类号： C12N15/82 , C07K14/415
摘要： The present invention is concerned with means and methods for allowing tissue specific and, in particular, seed specific expression of genes. The present invention, accordingly, relates to a polynucleotide comprising an expression control sequence which allows seed specific expression of a nucleic acid of interest being operatively linked thereto. Moreover, the present invention contemplates vectors, host cells, non-human transgenic organisms comprising the aforementioned polynucleotide as well as methods and uses of such a polynucleotide.

86. 发明专利

ES2653727T3 Desaturasas y proceso para la producción de ácidos grasos poliinsaturados en organismos transgénicos 未知
公开(公告)号：ES2653727T3
公开(公告)日：2018-02-08
申请号：ES13180237
申请日：2009-12-08
申请人： BASF PLANT SCIENCE GMBH
发明人： SENGER TORALF , BAUER JOERG , NAPIER JOHNATHAN A
IPC分类号： C12N15/82 , A01H5/00 , C12N9/02 , C12N15/53 , C12P7/64
摘要： Un polinucleótido que comprende una secuencia de ácidos nucleicos seleccionada del grupo que consiste de: (a) una secuencia de ácidos nucleicos como se muestra en la SEQ ID NO. 50 o 51; (b) una secuencia de ácidos nucleicos que codifica un polipéptido que caracteriza una secuencia de aminoácidos como se muestra en cualquiera de la SEQ ID NO. 52; (c) una secuencia de ácidos nucleicos que codifica un polipéptido con una secuencia de aminoácidos con por lo menos 75 % de identidad con la SEQ ID NO. 52, y que codifica un polipéptido con una actividad de omega-3- desaturasa; y (d) una secuencia de ácidos nucleicos obtenible u obtenida mediante la amplificación de ácido nucleico del material genético de Hyaloperonospora parasitica usando el primer par - SEQ ID NO. 53 y SEQ ID NO. 55, o - SEQ ID NO. 54 y SEQ ID NO. 56 y que codifica un polipéptido con una actividad de omega-3-desaturasa.

87. 发明专利

CA2479409C DOUBLE-STRANDED RIBONUCLEIC ACID CONSTRUCTS AND METHODS FOR REDUCING EXPRESSION OF GENES IN PLANTS 未知
公开(公告)号：CA2479409C
公开(公告)日：2018-01-09
申请号：CA2479409
申请日：2003-03-17
申请人： BASF PLANT SCIENCE GMBH
发明人： KOCK MICHAEL , BAUER JOERG
IPC分类号： C12N15/11 , A61K38/00 , C12N15/29 , C12N15/82
摘要： The present invention relates to a method for reducing the expression of more than two different endogenous target genes in a plant cell or a plant by introducing, into said plant cell or said plant, an at least partially double-stranded ribonucleic acid molecule, to a ribonucleic acid molecule and its use for preparing pharmaceuticals and generating plants with advantageous properties.

88. 发明专利

CA2694661C DESATURASES AND PROCESS FOR THE PRODUCTION OF POLYUNSATURATED FATTY ACIDS IN TRANSGENIC ORGANISMS 未知
公开(公告)号：CA2694661C
公开(公告)日：2017-10-03
申请号：CA2694661
申请日：2008-07-30
申请人： BASF PLANT SCIENCE GMBH
发明人： BAUER JOERG , SENGER TORALF
IPC分类号： C12N15/53 , C07K16/40 , C12N9/02 , C12N15/82 , C12P7/64
摘要： The invention relates to polynucleotides from Helobdella robusta, Laccaria bicolor, Lottia gigantea, Microcoleus chthonoplastes, Monosiga brevicollis, Mycosphaerella fijiensis, Mycospaerella graminicola, Naegleria gruben, Nectria haematococca, Nematostella vectensis, Phycomyces blakesleeanus, Trichoderma resii, Physcomitrella patens, Postia placenta, Selaginella moellendorffii and Microdochium nivale that code desaturases and that can be used for the recombinant production of polyunsaturated fatty acids. The invention also relates to the vectors, host cells and transgenic non-human organisms that contain said inventive polynucleotides, and also the polypeptides coded by said polynucleotides. The invention further relates to antibodies against the inventive polypeptides. Finally, the invention relates to production methods for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to the use thereof as medicments, cosmetics, food, animal food, preferably fish food or food supplements.

89. 发明专利

CA2573972C METHOD FOR INCREASING THE CONTENT OF POLYUNSATURATED LONG-CHAINED FATTY ACIDS IN TRANSGENIC ORGANISMS 未知
公开(公告)号：CA2573972C
公开(公告)日：2016-06-07
申请号：CA2573972
申请日：2005-07-15
申请人： BASF PLANT SCIENCE GMBH
发明人： CIRPUS PETRA , BAUER JOERG , QIU XIAO , VRINTEN PATRICIA
IPC分类号： C12N15/82 , A01H5/00 , C11B3/00 , C11C1/08 , C12N9/04 , C12N9/20 , C12N9/88 , C12N15/53 , C12N15/55 , C12N15/60 , C12P7/64
摘要： The invention relates to a method for increasing the content of polyunsaturated long-chained fatty acids in an organism, by introducing nucleic acid coding for polypeptides and/or proteins having phopholipase, ketoacyl-CoA-reductase and/or dehydratase activity into the organisms. Advantageously, said enzymes stem from ostreococcus or thraustochytrium. The invention also relates to nucleic acid sequences, nucleic acid structures, vectors and organisms containing the inventive nucleic acid sequences, vectors containing the nucleic acid sequences and/or the nucleic acid structures, and transgenic organisms containing the above-mentioned nucleic acid sequences, nucleic acid structures and/or vectors. Advantageously, said above-mentioned nucleic acid frequencies, nucleic acid structures and vectors can optionally be expressed in the organism along with additional nucleic acid sequences coding for the polypeptides and/or proteins of fatty acids or lipid metabolism biosynthesis. Another advantage of the nucleic acid sequences of the fatty acids or lipid metabolism is that they are coded for .DELTA.-9 elongase, .DELTA.-8-desaturase, a .DELTA.-5-desaturase, .DELTA.-4-desaturase, .DELTA.-12-desaturase, .DELTA.-5-elongase and/or .DELTA.-6-elongase activity. Said desaturases and elongases stem from organisms such as thalassiosira, euglena, isochrysis, physcomitrella, thraustochytrium, borago, phytophthora, crypthecodinium, oncorhynchus, primula, xenopus, ciona, arabidopsis, mortierella, caenorhabditis, phaeodactylum, ceratodon or ostreococcus. The invention further relates to oils, lipids, and/or fatty acids that are produced according the inventive method, and to the use thereof, in addition to the inventive unsaturated fatty acids and triglycerides having an increased content of unsaturated fatty acids and to the use thereof.

90. 发明专利

CA2665336C PROCESS FOR THE PRODUCTION OF POLYUNSATURATED FATTY ACIDS IN TRANSGENIC ORGANISMS 未知
公开(公告)号：CA2665336C
公开(公告)日：2016-01-05
申请号：CA2665336
申请日：2007-10-04
申请人： BASF PLANT SCIENCE GMBH
发明人： BAUER JOERG , WETJEN TOM
IPC分类号： C12N9/02 , A23D9/00 , C07K16/40 , C12N9/10 , C12N9/16 , C12N9/88 , C12N15/53 , C12N15/54 , C12N15/55 , C12N15/60 , C12P7/62 , C12P7/64
摘要： The present invention relates to polynucleotides from Ostreococcus lucimarinus which encode desaturases and elongases, and can be used for the recombinant production of polyunsaturated fatty acids. In addition, the invention relates to vectors, host cells and transgenic non-human organisms which contain the polynucleotides, and also the polypeptides encoded by the polynucleotides. Finally, the invention also relates to production processes for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions.

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