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    • 82. 发明申请
    • Method for quantifying biomolecules conjugated to a nanoparticle
    • 用于定量与纳米颗粒缀合的生物分子的方法
    • US20080274463A1
    • 2008-11-06
    • US11800360
    • 2007-05-04
    • Xiao-Bo ChenChristopher BieniarzMichael Farrell
    • Xiao-Bo ChenChristopher BieniarzMichael Farrell
    • C12Q1/68G01N1/00G01N33/00C12Q1/00G01N33/53G01N33/536G01N33/563G01N33/20C12Q1/37G01N21/76
    • C12Q1/37B82Y15/00G01N33/54313G01N33/582Y10T436/143333Y10T436/17Y10T436/25125
    • Disclosed embodiments concern quantifying a biomolecule conjugated to a nanoparticle. Quantifying typically comprises determining the number of biomolecules per nanoparticle. Any suitable biomolecule can be used, including but not limited to, amino acids, peptides, proteins, haptens, nucleic acids, oligonucleotides, DNA, RNA, and combinations thereof. A single type of biomolecule may be conjugated to the nanoparticle, more than one biomolecule of a particular class may be conjugated to the nanoparticle, or two or more classes of biomolecules may be conjugated to the nanoparticle. Certain disclosed embodiments comprise enzymatically or chemically digesting a biomolecule conjugated to the nanoparticle, or displacing a biomolecule using ligand-exchange chemistry. Where biomolecule concentrations are determined, any technique suitable for determining biomolecule concentration can be used, such as spectrophotometric techniques, including measuring tryptophan fluorescence and using a standard fluorescence intensity versus biomolecule concentration curve.
    • 公开的实施方案涉及量化与纳米颗粒缀合的生物分子。 量化通常包括确定每个纳米颗粒的生物分子的数量。 可以使用任何合适的生物分子,包括但不限于氨基酸,肽,蛋白质,半抗原,核酸,寡核苷酸,DNA,RNA及其组合。 单一类型的生物分子可以与纳米颗粒结合,特定类别的多于一个的生物分子可以与纳米颗粒缀合,或者两种或更多类别的生物分子可以与纳米颗粒缀合。 某些公开的实施方案包括酶或化学消化与纳米颗粒缀合的生物分子,或使用配体交换化学位移生物分子。 在确定生物分子浓度的情况下,可以使用适合于测定生物分子浓度的任何技术,例如分光光度技术,包括测量色氨酸荧光并使用标准荧光强度与生物分子浓度曲线。
    • 84. 发明授权
    • Mammalian endonucleases and methods of use
    • 哺乳动物内切核酸酶和使用方法
    • US07396669B2
    • 2008-07-08
    • US10880881
    • 2004-06-30
    • Michael N. BoddyVeronique BlaisXiao-Bo ChenClare H. McGowanPierre-Henri L. GaillardPaul R. Russell
    • Michael N. BoddyVeronique BlaisXiao-Bo ChenClare H. McGowanPierre-Henri L. GaillardPaul R. Russell
    • C12N9/16C07K14/00C12Q1/44C12P21/06C12N15/00C12N5/10C12N1/21C07H21/04
    • C12N9/22
    • Isolated mammalian Mus81-Eme endonuclease complexes comprise an Mus81 protein portion and an Eme protein portion. A method of identifying a chemical compound that modulates mammalian cellular response to DNA damage comprises contacting a chemical compound to be tested with a biochemical mixture containing an isolated mammalian Mus81-Eme1 endonuclease complex, a source of magnesium ion, and a suitable DNA substrate; measuring the activity level of mammalian Mus81-Eme endonuclease complex in the mixture; comparing the measured activity level to the activity level of a substantially similar mixture of isolated Mus81-Eme1 endonuclease, magnesium ion, and DNA substrate in the absence of the chemical compound to be tested; and selecting a chemical compound that increases or decreases the endonuclease activity. Isolated mammalian Eme1 and Eme2 proteins derived from humans and murine species and isolated nucleic acids encoding the proteins are also described.
    • 分离的哺乳动物Mus81-Eme内切核酸酶复合物包含Mus81蛋白质部分和Eme蛋白质部分。 鉴定调节哺乳动物细胞对DNA损伤的反应的化合物的方法包括将待测试的化合物与含有分离的哺乳动物Mus81-Eme1内切核酸酶复合物,镁离子源和合适的DNA底物的生化混合物接触; 测量混合物中哺乳动物Mus81-Eme核酸内切酶复合物的活性水平; 将测量的活性水平与不存在待测化学化合物的分离的Mus81-Eme1内切核酸酶,镁离子和DNA底物的基本相似的混合物的活性水平进行比较; 并选择增加或减少核酸内切酶活性的化合物。 还描述了衍生自人和鼠类物种的分离的哺乳动物Eme1和Eme2蛋白以及编码蛋白质的分离的核酸。
    • 87. 发明申请
    • System for controlling the display size of a formula bar in a spreadsheet
    • 用于控制电子表格中公式栏显示大小的系统
    • US20070016850A1
    • 2007-01-18
    • US11479116
    • 2006-06-29
    • Bo ChenHui WangQingyuan ZhangLi Wan
    • Bo ChenHui WangQingyuan ZhangLi Wan
    • G06F17/00
    • G06F17/246
    • The present invention discloses a system for controlling the display size of a formula bar in a spreadsheet. Application of this system will make operation of the spreadsheet more convenient. The system for controlling the display size of the formula bar and a formula bar module are parallel in a main window module of a spreadsheet application, and the system includes a sub-system I which controls the display state of the formula bar and a sub-system II which controls the display range of the specific content in the formula bar. The system continuously executes real-time detecting and measuring of the aforementioned formula bar module while the application is running, and acquires data on the size of the formula bar from the aforementioned formula bar module. If the acquired data exceed the prescribed range, then it issues a command to the aforementioned formula bar module requesting a change in the size of the formula bar. In the mean time, it issues a command to the aforementioned sub-system I and the aforementioned sub-system II.
    • 本发明公开了一种用于控制电子表格中公式栏的显示尺寸的系统。 该系统的应用将使电子表格的操作更加方便。 用于控制公式栏和公式栏模块的显示尺寸的系统在电子表格应用的主窗口模块中是并行的,并且系统包括控制公式栏的显示状态的子系统I, 系统II,其控制公式栏中特定内容的显示范围。 在应用程序运行时,系统连续执行上述公式栏模块的实时检测和测量,并从上述公式栏模块获取关于公式栏的大小的数据。 如果所获取的数据超过规定范围,则向上述公式栏模块发出请求改变公式栏大小的命令。 同时,它向上述子系统I和上述子系统II发出命令。
    • 88. 发明申请
    • RFID-enabled personal shopping assistant system and method
    • 支持RFID的个人购物助理系统和方法
    • US20060282334A1
    • 2006-12-14
    • US11432623
    • 2006-05-12
    • Chung-En KaoPi-Chuan HungBo-Chen WuLi-Juen TsengBing-Chen Kuo
    • Chung-En KaoPi-Chuan HungBo-Chen WuLi-Juen TsengBing-Chen Kuo
    • G06Q30/00
    • G06Q30/02G06Q30/0631G06Q30/0641
    • The present invention relates to a personal shopping assistant system applying radio frequency identification (RFID) technology, i.e. an RFID-enabled personal shopping assistant system. The system of the invention is comprised of a plurality of trade articles, each having an RFID tag attached thereon, at least a mobile device, each having an RIFD reader, and a commodity database, by which a custom service program with real-time personal shopping assistant ability is established. By the RFID-enabled personal shopping assistant system of the invention, a relating method can be established for enabling a service provider to successfully acquire the shopping behavior of its customers so as to proceed with a responsive promotion just in time. Moreover, a consumer can also benefit from the method, that is, a consumer is enabled to rapidly query and obtain his favor goods with respect to his personal preference among a great amount of similar goods. According to the shopping pattern established by the RFID-enabled personal shopping assistant system and method of the invention, not only the time that a consumer wasted in trying to find the goods of his favor can be reduced, but also the correlation between goods and the personal preferences of consumers can be enhanced.
    • 本发明涉及应用射频识别(RFID)技术的个人购物助理系统,即具有RFID功能的个人购物助理系统。 本发明的系统包括多个交易品,每个商品都具有附着在其上的RFID标签,至少一个移动装置,每个具有RIFD读取器和商品数据库,通过该商品数据库,具有实时个人的定制服务程序 购物助理能力建立。 通过本发明的RFID启用个人购物助理系统,可以建立一种相关方法,使得服务提供商能够成功获取其客户的购物行为,从而及时进行响应式促销。 此外,消费者也可以从该方法中受益,即消费者能够在大量类似商品之间快速查询和获取他的个人喜好的商品。 根据由RFID启用的个人购物助理系统和本发明的方法所建立的购物模式,不仅可以减少消费者浪费尝试寻找他的商品的时间,而且可以减少商品与 可以增强消费者的个人喜好。
    • 89. 发明申请
    • Mammalian endonucleases and methods of use
    • 哺乳动物内切核酸酶和使用方法
    • US20060003434A1
    • 2006-01-05
    • US10880881
    • 2004-06-30
    • Michael BoddyVeronique BlaisXiao-Bo ChenClare McGowanPierre-Henri GaillardPaul Russell
    • Michael BoddyVeronique BlaisXiao-Bo ChenClare McGowanPierre-Henri GaillardPaul Russell
    • C12N9/16C07H21/04C12P21/06C12N9/64
    • C12N9/22
    • Isolated mammalian Mus81-Eme endonuclease complexes comprise an Mus81 protein portion and an Eme protein portion. A method of identifying a chemical compound that modulates mammalian cellular response to DNA damage comprises contacting a chemical compound to be tested with a biochemical mixture containing an isolated mammalian Mus81-Eme1 endonuclease complex, a source of magnesium ion, and a suitable DNA substrate; measuring the activity level of mammalian Mus81-Eme endonuclease complex in the mixture; comparing the measured activity level to the activity level of a substantially similar mixture of isolated Mus81-Eme1 endonuclease, magnesium ion, and DNA substrate in the absence of the chemical compound to be tested; and selecting a chemical compound that increases or decreases the endonuclease activity. Isolated mammalian Eme1 and Eme2 proteins derived from humans and murine species and isolated nucleic acids encoding the proteins are also described.
    • 分离的哺乳动物Mus81-Eme内切核酸酶复合物包含Mus81蛋白质部分和Eme蛋白质部分。 鉴定调节哺乳动物细胞对DNA损伤的反应的化合物的方法包括将待测试的化合物与含有分离的哺乳动物Mus81-Eme1内切核酸酶复合物,镁离子源和合适的DNA底物的生化混合物接触; 测量混合物中哺乳动物Mus81-Eme核酸内切酶复合物的活性水平; 将测量的活性水平与不存在待测化学化合物的分离的Mus81-Eme1内切核酸酶,镁离子和DNA底物的基本相似的混合物的活性水平进行比较; 并选择增加或减少核酸内切酶活性的化合物。 还描述了衍生自人和鼠类物种的分离的哺乳动物Eme1和Eme2蛋白以及编码蛋白质的分离的核酸。
    • 90. 发明授权
    • SIM card connector
    • SIM卡连接器
    • US06974343B2
    • 2005-12-13
    • US10957180
    • 2004-09-30
    • Qisheng ZhengShaoping DuBo Chen
    • Qisheng ZhengShaoping DuBo Chen
    • H01R13/24H01R13/627H01R13/62
    • H01R13/2421H01R13/627
    • An electrical card connector (100) includes a base (1), a cover (2) and a pivot (3) for pivotally connecting the cover with the base. The base includes an insulative housing (11) and a number of conductive terminals received in the housing. The cover includes a top cover (21) and a bottom cover (22) defining a receptacle (20) for receiving a card (4). The cover defines a cutout (25) on the bottom cover facing to an upper surface of the base and has latching mechanism (26) extending from opposite sides of the cutout for latching with the base and for hold the cover in a locked position.
    • 电卡连接器(100)包括基座(1),盖(2)和用于将盖与基座枢转连接的枢轴(3)。 基座包括绝缘壳体(11)和容纳在壳体中的多个导电端子。 所述盖包括顶盖(21)和底盖(22),所述底盖限定用于容纳卡(4)的插座(20)。 所述盖在所述底盖上限定面向所述基座的上表面的切口(25),并且具有从所述切口的相对侧延伸的闩锁机构(26),用于与所述基座锁定并将所述盖保持在锁定位置。