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71. 发明申请

US20120088266A1 BIOREACTOR PROCESS FOR PRODUCTION OF HYDROGEN FROM BIOMASS 有权
标题翻译：用于从生物量生产氢的生物反应器方法
公开(公告)号：US20120088266A1
公开(公告)日：2012-04-12
申请号：US13320493
申请日：2010-05-14
申请人： Vincent Myles Gray
发明人： Vincent Myles Gray
IPC分类号： C12P3/00 , C12Q1/04
CPC分类号： C12P3/00 , C12M21/04 , C12M25/20 , C12M29/18 , C12P1/04 , C12R1/01
摘要： This invention relates to bioreactor processes, particularly bioreactor processes for the production of hydrogen gas from biomass, more particularly to bioreactor processes for the production of hydrogen gas employing a mixed anaerobic thermophilic bacterial consortium during the anaerobic fermentation of biomass.
摘要翻译：本发明涉及用于从生物质生产氢气的生物反应器方法，特别是用于生产氢气的生物反应器方法，更具体地涉及在生物质的厌氧发酵期间使用混合厌氧嗜热细菌菌体生产氢气的生物反应器方法。

72. 发明申请

US20110138684A1 Integrated Syngas Fermentation Process and System 审中-公开
标题翻译：综合合成气发酵过程与系统
公开(公告)号：US20110138684A1
公开(公告)日：2011-06-16
申请号：US12635432
申请日：2009-12-10
申请人： David R. KRANZ
发明人： David R. KRANZ
IPC分类号： C10L1/18 , C12P7/06 , C12M1/00
CPC分类号： C12M21/04 , C10G2300/1011 , C10J3/463 , C10J2300/0916 , C10J2300/0969 , C10J2300/1659 , C10J2300/1665 , C10J2300/1681 , C10J2300/1815 , C10L1/02 , C12M21/12 , C12M25/20 , C12M29/24 , C12P7/065 , C12P7/10 , C12P7/14 , Y02E50/16 , Y02E50/17 , Y02E50/18 , Y02P20/145 , Y02P30/20
摘要： In some embodiments, the present invention relates to methods (processes) of syngas fermentation involving an integral gasification process, and to corresponding systems for carrying out or implementing such methods. In such methods and systems of the present invention, carbon dioxide (CO2) produced during the fermentation of syngas is directed into the gasifier (e.g., as a motive gas or component thereof) where it enhances carbon monoxide (CO) production and mitigates char production.
摘要翻译：在一些实施方案中，本发明涉及涉及整体气化过程的合成气发酵的方法（方法），以及用于实施或实施这些方法的相应系统。在本发明的这些方法和系统中，在合成气发酵期间产生的二氧化碳（CO 2）被引导到气化器（例如，作为动力气体或其组分）中，其中它增强一氧化碳（CO）产生并减轻焦炭产生。

73. 发明授权

US06706521B2 Bed cleaning system for fluidized-bed bioreactors 有权
标题翻译：流化床生物反应器床清洁系统
公开(公告)号：US06706521B2
公开(公告)日：2004-03-16
申请号：US09930894
申请日：2001-08-16
申请人： Samuel Frisch
发明人： Samuel Frisch
IPC分类号： C12M114
CPC分类号： C12M47/02 , C12M25/20 , C12M39/00
摘要： A reaction chamber is adapted to contain a fluidized bed having a slurry of liquid, media and biomass. A lift and motive fluid are provided to urge slurry from the reaction chamber through a passage from a slurry inlet to a slurry discharge. The height of the slurry inlet is adjustable with respect to the bottom of the reaction chamber to help control the fluidized bed.
摘要翻译：反应室适于容纳具有液体，介质和生物质的浆液的流化床。提供升力和动力流体以通过从浆料入口到浆料排出的通道来迫使来自反应室的浆料。浆料入口的高度相对于反应室的底部是可调节的，以帮助控制流化床。

74. 发明授权

US06599735B1 Continuous fermentation system 失效
标题翻译：连续发酵系统
公开(公告)号：US06599735B1
公开(公告)日：2003-07-29
申请号：US09684855
申请日：2000-10-06
申请人： Attila Bartók , Thorsten Mueh , Markus Rueckel
发明人： Attila Bartók , Thorsten Mueh , Markus Rueckel
IPC分类号： C12M136
CPC分类号： C12M41/48 , C12M25/18 , C12M25/20 , C12M37/02 , C12M41/12 , C12P21/02 , Y10S435/81
摘要： A fermentation assembly comprising: (a) a vessel for culturing living cells; (b) at least two storage flasks in fluid communication with the vessel for supply of liquids and a first transport means for transferring the liquids from the storage flasks to the vessel; (c) individual appliances operably connected to the transport means for monitoring the supply of the contents of the storage flasks to the vessel; (d) a harvest flask in fluid communication with the vessel and a second transport means for transferring the fermentation broth from the vessel to the harvest flask; and (e) a device operably connected to the first transport means for controlling and maintaining a constant dilution rate in the vessel with varying rates of individual supply of liquid from the storage flasks to the vessel is disclosed.
摘要翻译：一种发酵装置，包括：（a）用于培养活细胞的容器; （b）至少两个与所述容器流体连通以供应液体的储存瓶和用于将所述液体从所述储存瓶转移到所述容器的第一输送装置; （c）可操作地连接到运输工具上用于监测储存瓶的内容物供应给容器的各种设备; （d）与容器流体连通的收获瓶和用于将发酵液从容器转移到收获瓶的第二输送装置; 公开了一种可操作地连接到第一输送装置上的装置，用于控制和保持容器中恒定的稀释速率，其中液体从储存瓶到容器的单独供应速率变化不大。

75. 发明授权

US5906940A Culturing cells on macroporous glass carriers coated with gelatin, extracellular matrix protein and stromal cells 失效
标题翻译：在涂有明胶，细胞外基质蛋白和基质细胞的大孔玻璃载体上培养细胞
公开(公告)号：US5906940A
公开(公告)日：1999-05-25
申请号：US602137
申请日：1996-02-15
申请人： Christian Wandrey , Manfred Biselli , Bernd Schroder , Hans-Joachim Schmoll
发明人： Christian Wandrey , Manfred Biselli , Bernd Schroder , Hans-Joachim Schmoll
IPC分类号： C12M1/04 , C12M1/40 , C12M3/06 , C12N5/06 , C12N5/08 , C12N11/02 , C12N11/14
CPC分类号： C12M25/20 , C12M25/14
摘要： Stem cells or other organ-function cells are cultivated in a fluidized bed system on macroporous glass carrier bodies treated with a structure protein such as gelatin and an extracellular matrix protein such as fibronectin, and coated with a stroma cell layer. Glass carriers coated with gelatin are added to a fluidized bed reactor, and a culture medium containing an extracellular matrix protein is added to bind the protein to the gelatin. Stromal cells are then added and the cells are cultured to immobilize the cells on the carriers containing the bound protein. Immature organ-function cells are added to the reactor, and while generating a fluidized bed of the carriers in the culture medium, the culture medium is recirculated from and to the reactor in a recirculation loop. Bubble-free aeration of the culture medium is effected to cultivate the immature organ-function cells on the carriers to obtain both mature differentiated organ-function cells and progenitor organ-function cells. Mature differentiated organ-function cells which are released from the carriers are harvested from the reactor while retaining the progenitor organ-function cells which adhere to the carriers to provide an equilibrium between the harvested mature differentiated organ-function cells, the progenitor cells and the immature cells added to the reactor. Growth and regulatory factors for cultivating organ-function cells can be produced by culturing immobilized stromal cells in the reactor in the absence of the organ-function cells.
摘要翻译：干细胞或其他器官功能细胞在用结构蛋白如明胶和细胞外基质蛋白如纤连蛋白处理的大孔玻璃载体上在流化床系统中培养，并涂覆有基质细胞层。将涂有明胶的玻璃载体加入到流化床反应器中，并加入含有细胞外基质蛋白的培养基以将蛋白质与明胶结合。然后加入基质细胞，并培养细胞以将细胞固定在含有结合蛋白的载体上。将未成熟的器官功能细胞加入到反应器中，并且在培养基中产生载体的流化床的同时，培养基在循环回路中从反应器再循环。培养培养基的无气泡通气培养载体上的未成熟器官功能细胞以获得成熟分化的器官功能细胞和祖细胞器官功能细胞。从反应器中收集从载体释放的成熟的分化器官功能细胞，同时保留粘附于载体的祖细胞器官功能细胞，以在收获的成熟分化的器官功能细胞，祖细胞和未成熟细胞之间提供平衡将细胞加入到反应器中。培养器官功能细胞的生长和调节因子可以通过在不存在器官功能细胞的情况下在反应器中培养固定的基质细胞来产生。

76. 发明授权

US5811259A Biochemical reactor 失效
标题翻译：生化反应器
公开(公告)号：US5811259A
公开(公告)日：1998-09-22
申请号：US902092
申请日：1997-07-29
申请人： Peter Jans Hall
发明人： Peter Jans Hall
IPC分类号： C12M1/08 , C12M1/40 , C12M3/00 , B01F3/00
CPC分类号： C12M23/34 , C12M21/04 , C12M25/20 , C12M27/20 , C12M27/24 , C12M29/04 , C12M29/18 , Y10S210/903 , Y10S435/801
摘要： A biochemical reactor utilizing an immobilized carrier which is useful in applications such as denitrification of waste water and includes a circulation conduit within the interior of the biochemical reactor. A feed conduit feeds recirculating fluid through a circulation inlet of the circulation conduit. The feed outlet of the feed conduit is located relative to the circulation inlet opening to direct fluid flowing out of the feed outlet into the circulation conduit to induce a circulation motion of immobilized carriers and fluid into the circulation inlet opening, through the circulation conduit, out of a circulation outlet opening, through the interior of the biochemical reactor, and again into the inlet of the circulation conduit.
摘要翻译：一种利用固定化载体的生化反应器，其可用于诸如废水的脱氮等应用，并且包括在生化反应器内部的循环导管。进料管道将循环流体通过循环管道的循环入口进料。进料管道的进料出口相对于循环入口开口定位，以将从进料出口流出的流体引导到循环管道中，以引导固定的载体和流体循环通过循环管道流出循环入口的循环出口开口，通过生化反应器的内部，并再次进入循环管道的入口。

77. 发明授权

US5788842A Biomass separation apparatus and method 失效
标题翻译：生物质分离装置及方法
公开(公告)号：US5788842A
公开(公告)日：1998-08-04
申请号：US715561
申请日：1996-09-18
申请人： Sam Frisch
发明人： Sam Frisch
IPC分类号： C02F3/12 , C02F3/22 , C12M1/26 , C12M1/40 , C02F3/08
CPC分类号： C02F3/1226 , C02F3/223 , C12M25/20 , C12M33/14 , Y02W10/15
摘要： A separator for use with a fluidized-bed bioreactor. A lift draws a slurry of liquid, media and biomass from the fluidized bed. A biomass discharge is connected to the lift and located above the height of the fluidized bed for discharging excess biomass. A media discharge, also connected to the lift, discharges media from the slurry and returns the media to the fluidized bed.
摘要翻译：用于流化床生物反应器的分离器。电梯从流化床吸取液体，介质和生物质的浆液。生物质排放物连接到升降机并且位于流化床的高度之上以排出多余的生物量。还连接到电梯的介质排放物从浆液中排出介质并将介质返回到流化床。

78. 发明授权

US4983517A Reacting materials 失效
标题翻译：反应材料
公开(公告)号：US4983517A
公开(公告)日：1991-01-08
申请号：US899177
申请日：1986-08-22
申请人： Byung C. Kim , Dewey D. Y. Ryu
发明人： Byung C. Kim , Dewey D. Y. Ryu
IPC分类号： B01J8/22 , C12M1/08
CPC分类号： B01J8/222 , C12M25/20 , C12M27/24 , C12M29/18 , C12M41/24
摘要： Methods and apparatus for reacting materials to provide a product. In a reactor (20) having an inner column (A) and an adjacent coaxial outer column (B), and having an upper zone (II) and an adjacent lower zone (I), both zones (I,II) are filled with a liquid (C). A gas (4) is fed into the liquid (C) at the lower end of the upper zone (II). The liquid (C) and a part of the gas (4) are circulated generally upward in one column (A or B) and generally downward in the other column (B or A) in the upper zone (II). Particles (15) of a solid substance are provided in a column (A or B) in the lower zone (I). The liquid (C) is circulated between the lower zone (I) and the upper zone (II) in such manner that the liquid (C) contacts and fluidizes the solid particles (15) while confining them to the lower zone (I), while the gas (4) contacts the liquid (C) and is confined to the upper zone (II).
摘要翻译：用于使材料反应以提供产品的方法和装置。在具有内柱（A）和相邻同轴外柱（B）的具有上区（II）和相邻下区（I）的反应器（20）中，两个区（I，II）都填充有液体（C）。气体（4）在上部区域（II）的下端被供给到液体（C）中。液体（C）和气体（4）的一部分在上部区域（II）中的一列（A或B）中大致向上循环并且在另一列（B或A）中大体向下循环。在下部区域（I）的列（A或B）中设置固体物质（15）。液体（C）在下部区域（I）和上部区域（II）之间循环，使得液体（C）在使其固定在下部区域（I）的同时使固体颗粒（15）接触并流化，而气体（4）接触液体（C）并限制在上部区域（II）。

79. 发明授权

US4978616A Fluidized cell cultivation process 失效
标题翻译：流化细胞培养过程
公开(公告)号：US4978616A
公开(公告)日：1990-12-18
申请号：US92599
申请日：1987-09-03
申请人： Robert C. Dean, Jr. , Peter V. Grela , Subhash B. Karkare , Peter W. Runstadler, Jr.
发明人： Robert C. Dean, Jr. , Peter V. Grela , Subhash B. Karkare , Peter W. Runstadler, Jr.
IPC分类号： C07K14/59 , C12M1/40 , C12M3/02 , C12N5/00 , C12P7/06
CPC分类号： C12P7/06 , C07K14/59 , C12M23/58 , C12M25/20 , C12M27/02 , C12N5/0075 , C12N2531/00 , Y02E50/17 , Y10S435/813 , Y10S435/819
摘要： A fluidized bed reactor and reaction process, particularly well-suited for culturing cells, for example, for tissue culture and fermentation processes, are described which involve the treatment of at least a portion of the fluid exiting the fluidized bed reactor in a side loop in a manner to alter its temperature or composition, e.g., oxygenation, with recirculation of this treated fluid to the reactor as a portion of the fluid causing bed fluidization.
摘要翻译：描述了特别适用于培养细胞的流化床反应器和反应方法，例如用于组织培养和发酵方法，其涉及在侧循环中处理离开流化床反应器的至少一部分流体一种改变其温度或组成，例如氧合的方式，将该处理过的流体再循环到反应器中作为导致床流化的流体的一部分。

80. 发明授权

US4806477A Process for reacting substrates with biocatalysts in a 2-phase liquid system 失效
标题翻译：使底物与生物催化剂在2相液体体系中反应的方法
公开(公告)号：US4806477A
公开(公告)日：1989-02-21
申请号：US055541
申请日：1987-05-29
申请人： Reinhardt Rothert , Dieter Wullbrandt
发明人： Reinhardt Rothert , Dieter Wullbrandt
IPC分类号： C12M1/02 , C12M1/40 , C12N11/02 , C12P41/00
CPC分类号： C12M41/22 , C12M25/20 , C12M27/02 , C12M29/04 , C12M29/18 , C12M33/22
摘要： A process for reacting substrates with biocatalysts in a two-phase liquid system disposed in a columnar vessel having a top portion with an exit opening and a first filter disposed therein, a bottom portion with an entrance opening and a second filter disposed therein, a feed line connecting the exit and entrance openings, and a pump disposed in the feed line. The process comprises the steps of: stirring the two-phase liquid in the columnar vessel to accelerate sedimentation and minimize accumulation of the biocatalysts in the top portion of the vessel; selectively controlling the temperature of the two-phase liquid within the vessel; drawing, with the pump, a portion of the two-phase liquid through the exit opening and first filter; and passing the drawn-off portion of the two-phase liquid through the feed line and through the entrance opening and second filter into the vessel to enhance miscibility of the individual phases of the two-phase liquid with the vessel.
摘要翻译：一种将底物与生物催化剂反应在两相液体系统中的方法，所述两相液体系统设置在具有顶部的柱状容器中，所述顶部具有出口和设置在其中的第一过滤器，底部具有入口和设置在其中的第二过滤器，连接出口和入口的管线，以及设置在进料管线中的泵。该方法包括以下步骤：搅拌柱状容器中的两相液体以加速沉淀并最小化生物催化剂在容器顶部的积聚; 选择性地控制容器内的两相液体的温度; 通过泵抽出一部分两相液体通过出口和第一过滤器; 并将两相液体的引出部分通过进料管线并通过入口和第二过滤器进入容器，以增强两相液体的各相与容器的混溶性。

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