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    • 3. 发明授权
    • Process for reacting substrates with biocatalysts in a 2-phase liquid
system
    • 使底物与生物催化剂在2相液体体系中反应的方法
    • US4806477A
    • 1989-02-21
    • US055541
    • 1987-05-29
    • Reinhardt RothertDieter Wullbrandt
    • Reinhardt RothertDieter Wullbrandt
    • C12M1/02C12M1/40C12N11/02C12P41/00
    • C12M41/22C12M25/20C12M27/02C12M29/04C12M29/18C12M33/22
    • A process for reacting substrates with biocatalysts in a two-phase liquid system disposed in a columnar vessel having a top portion with an exit opening and a first filter disposed therein, a bottom portion with an entrance opening and a second filter disposed therein, a feed line connecting the exit and entrance openings, and a pump disposed in the feed line. The process comprises the steps of: stirring the two-phase liquid in the columnar vessel to accelerate sedimentation and minimize accumulation of the biocatalysts in the top portion of the vessel; selectively controlling the temperature of the two-phase liquid within the vessel; drawing, with the pump, a portion of the two-phase liquid through the exit opening and first filter; and passing the drawn-off portion of the two-phase liquid through the feed line and through the entrance opening and second filter into the vessel to enhance miscibility of the individual phases of the two-phase liquid with the vessel.
    • 一种将底物与生物催化剂反应在两相液体系统中的方法,所述两相液体系统设置在具有顶部的柱状容器中,所述顶部具有出口和设置在其中的第一过滤器,底部具有入口和设置在其中的第二过滤器, 连接出口和入口的管线,以及设置在进料管线中的泵。 该方法包括以下步骤:搅拌柱状容器中的两相液体以加速沉淀并最小化生物催化剂在容器顶部的积聚; 选择性地控制容器内的两相液体的温度; 通过泵抽出一部分两相液体通过出口和第一过滤器; 并将两相液体的引出部分通过进料管线并通过入口和第二过滤器进入容器,以增强两相液体的各相与容器的混溶性。