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61. 发明申请

US20080032335A1 Polypeptide Having Activity of Unsaturating W3-Fatty Acid, Polynucleotide Coding for the Polypeptide and Use Thereof 有权
标题翻译：具有不饱和W3-脂肪酸活性的多肽，用于多肽的多核苷酸编码及其用途
公开(公告)号：US20080032335A1
公开(公告)日：2008-02-07
申请号：US11660093
申请日：2005-08-19
申请人： Sakayu Shimizu , Eiji Sakuradani
发明人： Sakayu Shimizu , Eiji Sakuradani
IPC分类号： C12N15/09 , A23L1/30 , C07H21/04 , C07K14/37 , C12N5/10 , C12P7/64 , C12P21/00 , C12N15/63 , C07K16/14 , C12N1/15
CPC分类号： C12P7/6427 , C12N9/0083 , C12P7/6409
摘要： The present invention provides a polypeptide having a wide substrate specificity and having a ω3 fatty acid desaturation activity, which makes efficiently unsaturated bond at ω3 position, and a polynucleotide coding for the same. By expressing the polypeptide in the organism, mass production of n-3 series PUFAs is enabled. Specifically, the polypeptide having a ω3 fatty acid desaturation activity and consisting of the amino acid sequence represented by SEQ ID NO: 1, a polynucleotide coding for a polypeptide having a ω3 fatty acid desaturation activity that consists of the base sequence represented by SEQ ID NO: 2 or SEQ ID NO: 3, and the like is useful for production of n-3 series fatty acids.
摘要翻译：本发明提供了具有宽底物特异性并具有ω3脂肪酸去饱和活性的多肽，其在ω3位上有效地形成不饱和键，以及编码该多肽的多核苷酸。通过在生物体中表达多肽，可以批量生产n-3系PUFA。具体地说，具有ω3脂肪酸去饱和活性的多肽，由SEQ ID NO：1表示的氨基酸序列组成，该多核苷酸编码具有ω3脂肪酸去饱和活性的多肽，其由SEQ ID NO：：2或SEQ ID NO：3等可用于生产n-3系列脂肪酸。

62. 发明申请

US20070026510A1 Process for biochemical production of glyoxylic acid 审中-公开
标题翻译：乙醛酸生化生产工艺
公开(公告)号：US20070026510A1
公开(公告)日：2007-02-01
申请号：US10544618
申请日：2004-02-13
申请人： Akira Iwasaki , Takehiro Matsumoto , Motohisa Washida , Hiroshi Watanabe , Junzou Hasegawa , Sakayu Shimizu
发明人： Akira Iwasaki , Takehiro Matsumoto , Motohisa Washida , Hiroshi Watanabe , Junzou Hasegawa , Sakayu Shimizu
IPC分类号： C12P7/50 , C12N1/20
CPC分类号： C12P7/40
摘要： The present invention provides an industrially advantageous process for biochemical production of glyoxylic acid from glyoxal. More specifically, the present invention provides a process for production of glyoxylic acid, which is characterized in that the process comprises allowing oxidoreductase that can convert glyoxal into glyoxylic acid, such as oxidase and dehydrogenase, to act on glyoxal, so as to convert glyoxal into glyoxylic acid.
摘要翻译：本发明提供了从乙二醛生物化生产乙醛酸的工业上有利的方法。更具体地说，本发明提供了一种生产乙醛酸的方法，其特征在于该方法包括允许将乙二醛转化为乙醛酸（如氧化酶和脱氢酶）的氧化还原酶作用于乙二醛，从而将乙二醛转化为乙醛酸。

63. 发明授权

US07166452B2 Aminoketone asymmetric reductase and nucleic acid thereof 有权
标题翻译：氨基酮不对称还原酶及其核酸
公开(公告)号：US07166452B2
公开(公告)日：2007-01-23
申请号：US10469682
申请日：2002-03-01
申请人： Keiji Sakamoto , Shinji Kita , Kazuya Tsuzaki , Tadanori Morikawa , Sakayu Shimizu , Michihiko Kataoka
发明人： Keiji Sakamoto , Shinji Kita , Kazuya Tsuzaki , Tadanori Morikawa , Sakayu Shimizu , Michihiko Kataoka
IPC分类号： C12N9/02 , C12P13/00 , C12P1/06 , C12P1/04
CPC分类号： C12N9/0006 , C12P13/001 , C12P13/008
摘要： A protein, which is an aminoketone asymmetric reductase, having an effect of producing d-pseudoephedrine by acting on 1-2-methylaminopropiophenone, and having the following physiochemical properties: substrate: 1-2-methylaminopropiophenone optimum pH: pH 8.1 optimum temperature: 55° C. coenzyme: NADP molecular weight: about 28500 Da homotetramer and a nucleic acid coding the protein.
摘要翻译：作为氨基酮不对称还原酶的蛋白质，具有通过作用于1-2-甲基氨基苯丙酮而产生d-伪麻黄碱的作用，具有以下物理化学性质：底物：1-2-甲基氨基苯丙酮最适pH：pH 8.1最适温度：55 ℃。辅酶：NADP分子量：约28500Da同型四聚体和编码该蛋白质的核酸。

64. 发明授权

US07157254B1 Microorganism secreting out lipid and process for producing the lipid and lipid balls having the lipid encapsulating therein by using the mircroorganism 有权
标题翻译：分泌脂质的微生物和通过使用微生物制备脂质和脂质球的方法，其中脂质和脂质包封在其中
公开(公告)号：US07157254B1
公开(公告)日：2007-01-02
申请号：US09807541
申请日：2000-08-11
申请人： Kengo Akimoto , Hiroshi Kawashima , Sakayu Shimizu
发明人： Kengo Akimoto , Hiroshi Kawashima , Sakayu Shimizu
IPC分类号： C12P7/64
CPC分类号： A61K31/202 , A23C9/1528 , A23C11/04 , A23D9/00 , A23K20/158 , A23L2/52 , A23L5/00 , A23L31/00 , A23L33/12 , A23L33/14 , A23V2002/00 , A61K8/14 , A61K8/92 , A61K8/925 , A61K8/99 , A61K31/201 , A61Q19/00 , C12P7/6472 , C12R1/645 , A23V2200/224 , A23V2250/18 , A23V2250/1862 , A23V2250/187 , A23V2250/1868
摘要： There are provided microorganisms having a property of producing a lipid containing unsaturated fatty acids as constituent fatty acids and extracellularly secreting the produced lipid encapsulated in lipid vesicles, methods of screening said microorganisms, as well as methods of efficiently producing a fatty acid-containing lipid using said microorganisms. Furthermore, there are provided lipid vesicles encapsulating a lipid containing unsaturated fatty acids, and foods, cosmetics, and animal feeds comprising said lipid vesicles added thereto. Artificially treated microorganisms or microorganisms collected from nature are grown on a solid medium, and microbial strains that form lipid vesicles at the periphery of the colonies and/or microbial strains that, when cultured in a transparent liquid medium, make the culture liquid cloudy are selected. The microorganisms obtained are cultured, lipid-containing lipid vesicles secreted in the culture liquid, are separated from the culture liquid, and the lipid is separated and purified.
摘要翻译：提供了具有产生含有不饱和脂肪酸的脂质作为构成脂肪酸并细胞外分泌包封在脂质囊泡中的所产生的脂质的性质的微生物，筛选所述微生物的方法以及使用以下方法有效生产含脂肪酸的脂质：说微生物。此外，提供了包含含有不饱和脂肪酸的脂质的脂质囊泡，以及包含所述脂质囊泡的食品，化妆品和动物饲料。人造处理的微生物或自然界收集的微生物在固体培养基上生长，并且在菌落和/或微生物菌株的周围形成脂质囊泡的微生物菌株，当在透明液体培养基中培养时，选择培养液混浊。培养得到的微生物，分泌到培养液中的含有脂质的脂质囊泡与培养液分离，分离纯化脂质。

65. 发明授权

US07091244B1 Process for preparing fat or oil containing unsaturated fatty acid 有权
标题翻译：制备含不饱和脂肪酸的油脂的方法
公开(公告)号：US07091244B1
公开(公告)日：2006-08-15
申请号：US09254152
申请日：1997-08-27
申请人： Kenichi Higashiyama , Kengo Akimoto , Sakayu Shimizu
发明人： Kenichi Higashiyama , Kengo Akimoto , Sakayu Shimizu
IPC分类号： A61K31/20 , A61K31/22 , C12P7/62 , C12P7/64 , C07C53/00
CPC分类号： C12P7/6472 , A23D9/00 , A23K20/158 , A23L33/12 , A23L33/40 , A23V2002/00 , C12P7/64 , Y10S426/801 , Y10S426/807 , A23V2250/54252 , A23V2250/1874 , A23V2250/1868 , A23V2250/1862 , A23V2250/187 , A23V2250/612 , A23V2250/28 , A23V2250/70 , A23V2250/156
摘要： A process for production of unsaturated fatty acid-containing oils which is characterized by culturing a microorganism belonging to the genus Mortierella subgenus Mortierella in a medium containing a nitrogen source derived from soybean, and collecting the unsaturated fatty acid-containing oil from the cultured product. The oils are obtained with a low 24,25-methylenecholest-5-en-3β-ol content.
摘要翻译：一种生产含不饱和脂肪酸的油的方法，其特征在于在含有源自大豆的氮源的培养基中培养属于被孢霉属的被孢霉属的被孢霉属的微生物，并从培养产物中收集不饱和脂肪酸的油。获得的油具有低的24,25-亚甲基胆甾-5-烯-3β-醇含量。

66. 发明申请

US20060121587A1 Process for actinol production from ketoisophorone 审中-公开
标题翻译：酮异二酮生产肌醇六聚体的方法
公开(公告)号：US20060121587A1
公开(公告)日：2006-06-08
申请号：US10528843
申请日：2003-09-16
申请人： Tatsuo Hoshino , Yutaka Setoguchi , Sakayu Shimizu , Kazuyuki Tabata
发明人： Tatsuo Hoshino , Yutaka Setoguchi , Sakayu Shimizu , Kazuyuki Tabata
IPC分类号： C12P7/26 , C12N1/21 , C12N15/74 , C12N1/18
CPC分类号： C12R1/645 , C12P7/26 , C12R1/15 , C12R1/74 , C12R1/865
摘要： Disclosed is a process for producing actinol from ketoisophorone which comprises contacting ketoisophorone with a recombinant microorganism or cell-free extract thereof in a reaction mixture, wherein said recombinant microorganism is obtainable by transforming a host microorganism, e.g. selected from the group consisting of microorganisms of the genera Saccharomyces, Zygosaccharomyces, and Candida, such as commercially available baker's yeast, Saccharomyces cerevisiae ATCC7754, Saccharomyces rouxii (Zygosaccharomyces rouxii) HUT7191 (IFO 0494), Saccharomyces delbrueckii HUIT7116 (Saccharomyces unisporus IFO 0298), Saccharomyces delbrueckii (Torulaspora delbrueckii) HUT7102, Saccharomyces willianus HU7106, Zygosaccharomyces bailii ATCC11486, Candida tropicalis IFO 1403, and a mutant thereof, which is capable of reducing ketoisophorone to levodione with a levodione reductase gene, e.g. a levodione reductase gene derived from a microorganism belonging to the genus Corynebacterium, such as C. aquaticum AKU611 (FERM BP-6448) or a mutant thereof, and isolating the produced actinol from the reaction mixture.
摘要翻译：本发明公开了一种由酮双异佛尔酮制备锕系元素的方法，该方法包括将酮异二氢喋呤与重组微生物或其无细胞提取物在反应混合物中接触，其中所述重组微生物可通过将宿主微生物，选自由酵母属，接合酵母属和假丝酵母属的微生物组成的组，例如可商购的面包酵母，酿酒酵母ATCC7754，酿酒酵母（Zogosaccharomyces rouxii）HUT7191（IFO 0494），酿酒酵母HUIT7116（Saccharomyces unisporus IFO 0298），酿酒酵母（Torulaspora delbrueckii）HUT7102，酿酒酵母HU7106，接合酵母属酵母ATCC11486，热带假丝酵母IFO 1403及其突变体，其能够用左旋二酮还原酶基因将酮异二酮降低成左旋多糖，例如衍生自属于棒状杆菌属的微生物，例如C.Actatica AKU611（FERM BP-6448）或其突变体的左旋体还原酶基因，并从反应混合物中分离生成的锕系元素。

67. 发明申请

US20050266538A1 Process for producing arachidonic acid-containing lipids and dihomo-gamma-linolenic acid containing lipids 失效
标题翻译：含有花生四烯酸的脂质和含有dihomo-γ-亚麻酸的脂质的制备方法
公开(公告)号：US20050266538A1
公开(公告)日：2005-12-01
申请号：US11167224
申请日：2005-06-28
申请人： Kengo Akimoto , Kenichi Higashiyama , Sakayu Shimizu
发明人： Kengo Akimoto , Kenichi Higashiyama , Sakayu Shimizu
IPC分类号： C12P7/64 , C12R1/645 , C12N1/16
CPC分类号： C12P7/6472 , Y10S435/911
摘要： A process for producing lipids containing arachidonic acid comprising culturing a microorganism in which ω3 desaturase activity has been decreased or is lacking at a temperature lower than the optimum growth temperature from the start of culturing or after culturing at the optimum growth temperature, said microorganism being obtained by the mutation treatment of a microorganism capable of producing arachidonic acid and belonging to the genus Mortierella and the like; and then recovering lipids containing arachidonic acid from the culture.
摘要翻译：一种含花生四烯酸的脂质的制造方法，其特征在于，在培养开始时或培养后，在最适生长温度下培养微生物，其中ω3去饱和酶活性降低或不足于最佳生长温度的温度，所述微生物获得通过突变处理能够产生花生四烯酸并属于被孢霉属等的微生物; 然后从培养物中回收含有花生四烯酸的脂质。

68. 发明申请

US20050106696A1 Enone reductase 有权
标题翻译： Enone还原酶
公开(公告)号：US20050106696A1
公开(公告)日：2005-05-19
申请号：US10505396
申请日：2003-02-14
申请人： Michihiko Kataoka , Sakayu Shimizu
发明人： Michihiko Kataoka , Sakayu Shimizu
IPC分类号： C12N9/02 , C12P7/26 , C12R1/72 , C07H21/04 , C12N1/18 , C12N15/74
CPC分类号： C12N9/001 , C12P7/26
摘要： An isolated enone reductase characterized by a molecular mass of 61300+/−5000 Da; NADPH and NADPH as co-factor; a temperature optimum of 55-60° C. at pH 7.4; a pH optimum of 4.5-8.5 and substrate specificity on ∝,β-unsatured ketones; a process for producing it from a microorganism and a process for producing levodione from ketoisophorone using such reductase.
摘要翻译：分离的烯酮还原酶，其特征在于分子量为61300 +/- 5000Da; NADPH和NADPH作为辅因子; 温度最适为55-60℃，pH 7.4; pH最适为4.5-8.5，底物特异性为α，β-不熟酮; 从微生物生产它的方法和使用这种还原酶从酮异二酮生产左旋二酮的方法。

69. 发明授权

US06893861B1 Method for producing trifluoro-3(r)-hydroxybutyric acid derivatives 失效
标题翻译：制备三氟-3（r） - 羟基丁酸衍生物的方法
公开(公告)号：US06893861B1
公开(公告)日：2005-05-17
申请号：US09622385
申请日：1999-02-18
申请人： Michael Petersen , Olwen Birch , Sakayu Shimizu , Andreas Kjener , Marie-Luise Hischier , Susanne Thöni
发明人： Michael Petersen , Olwen Birch , Sakayu Shimizu , Andreas Kjener , Marie-Luise Hischier , Susanne Thöni
IPC分类号： C12N15/09 , C12N9/02 , C12N9/04 , C12N15/53 , C12P7/42 , C12P7/62 , C12P11/00 , C12P13/02 , C12R1/19 , C12P7/40
CPC分类号： C12N9/0008 , C12N9/0006 , C12P7/42 , C12P7/62 , C12P11/00 , C12P13/02
摘要： The invention relates to a biotechnological method for producing trifluoro-3(R)-hydroxybutyric acid derivatives of the general formula (I), where R1 represents —OR2, where R2 is hydrogen, C1-10 alkyl, C1-10 alkenyl, C3-8 cy-cloalkyl, aryl, alkoxyalkyl or alkoxyalkoxyalkyl; —NR3R4, where R3 and R4 are the same or different and represent hydrogen, C1-10 alkyl, C1-10 alkenyl, C3-8 cycloalkyl or aryl; or —SR5, where R5 represents hydrogen, C1-10 alkyl, C1-10 alkenyl, aryl or C3-8 cycloalkyl, based on a trifluoroacetoacetic acid derivative of the general formula (II), where R1 has the meaning given above, by means of micro-organisms which are able to reduce a carbonyl function or by means of a cell-free enzyme extract of said micro-organisms.
摘要翻译：本发明涉及一种生产通式（I）的三氟-3（R） - 羟基丁酸衍生物的生物技术方法，其中R 1表示-OR 2，其中 R 2是氢，C 1-10烷基，C 1-10 1-10烯基，C 3-8环芳基，烷氧基烷基或烷氧基烷氧基烷基; 其中R 3和R 4相同或不同并且表示氢，C 3和R 4相同或不同，表示氢，C C 1-10烷基，C 1-10 1-10烯基，C 3-8环烷基或芳基; 或-SR 5，其中R 5表示氢，C 1-10烷基，C 1-10 1-10烯基，芳基或C 3-8环烷基，基于通式（II）的三氟乙酰乙酸衍生物，其中R 1具有上述含义，通过微量 - 能够降低羰基官能团或通过所述微生物的无细胞酶提取物的有机体。

70. 发明授权

US06673583B2 Recombinantly producing levodione reductase 失效
标题翻译：重组产左旋酮还原酶
公开(公告)号：US06673583B2
公开(公告)日：2004-01-06
申请号：US10327726
申请日：2002-12-23
申请人： Sakayu Shimizu , Masaru Wada
发明人： Sakayu Shimizu , Masaru Wada
IPC分类号： C12P724
CPC分类号： C12N9/0008 , C12P7/26
摘要： The present invention is directed to genetic material useful for the preparation of actinol, such as an isolated DNA including a nucleotide sequence coding for an enzyme having levodione reductase activity, a polypeptide encoded by such a DNA, recombinant organisms, and the like. These genetic materials may originate from Corynebacterium, Cellulomonas, Planococcus, Arthrobacter, and the like. The present invention also provides a process for the production of actinol.
摘要翻译：本发明涉及可用于制备锕系元素的遗传物质，例如分离的DNA，包括编码具有左旋体还原酶活性的酶的核苷酸序列，由这种DNA编码的多肽，重组生物等。这些遗传物质可以来自棒状杆菌属，丝杆菌属，球菌属，节杆菌属等。本发明还提供了制备肌动蛋白的方法。

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