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61. 发明授权

US07923551B2 Method of purifying RNA using kosmotropic salt 有权
标题翻译：使用去离子水纯化RNA的方法
公开(公告)号：US07923551B2
公开(公告)日：2011-04-12
申请号：US12110391
申请日：2008-04-28
申请人： Myo-yong Lee , Nam Huh , Joon-ho Kim
发明人： Myo-yong Lee , Nam Huh , Joon-ho Kim
IPC分类号： C07H21/00
CPC分类号： C07H21/02 , C12N15/1003 , C12N15/1006
摘要： The present invention provides a method of purifying RNA, including contacting a solid support with an acidic solution having a RNA-containing sample and a kosmotropic salt having a concentration of less than 1M, thereby binding the RNA to the solid support. According to the present invention, RNA is purified efficiently due to high RNA yield and low contamination by DNA. The present invention is particularly effective in purifying RNAs of 200 nucleotides or less.
摘要翻译：本发明提供了一种纯化RNA的方法，包括使固体支持物与具有含RNA样品的酸性溶液和浓度小于1M的感应性盐接触，从而将RNA与固体支持物结合。根据本发明，由于高RNA产量和低DNA污染，RNA被有效地纯化。本发明在纯化200个或更少核苷酸的RNA中特别有效。

62. 发明申请

US20070068813A1 Microfluidic device for electrically regulating the pH of a fluid therein and method of regulating the pH of a fluid in a microfluidic device using the same 有权
标题翻译：用于电调节其中流体pH值的微流体装置及使用其调节微流体装置中的流体pH值的方法
公开(公告)号：US20070068813A1
公开(公告)日：2007-03-29
申请号：US11472182
申请日：2006-06-21
申请人： Jung-im Han , Joon-ho Kim
发明人： Jung-im Han , Joon-ho Kim
IPC分类号： C07K1/26 , G01N27/00
CPC分类号： G05D21/02
摘要： Provided is a microfluidic device for electrically regulating the pH of a fluid comprising: a first chamber comprising a first electrode; a second chamber comprising a second electrode; a third chamber comprising a third electrode, a metal ion exchange membrane between the first and second chambers; and a hydrogen ion exchange membrane between the first or second chamber and the third chamber.
摘要翻译：提供一种用于电流调节流体pH的微流体装置，包括：第一室，包括第一电极; 第二室，包括第二电极; 第三室，包括第三电极，在第一和第二室之间的金属离子交换膜; 以及在第一或第二室与第三室之间的氢离子交换膜。

63. 发明申请

US20070017810A1 Microfluidic device for electrochemically regulating pH of fluid therein and method of regulating pH of fluid using the microfluidic device 有权
标题翻译：用于电化学调节流体pH值的微流体装置和使用微流体装置调节流体pH值的方法
公开(公告)号：US20070017810A1
公开(公告)日：2007-01-25
申请号：US11489289
申请日：2006-07-19
申请人： Hun-joo Lee , Joon-ho Kim , Chin-sung Park , Young-sun Lee , Jong-Suk Chung
发明人： Hun-joo Lee , Joon-ho Kim , Chin-sung Park , Young-sun Lee , Jong-Suk Chung
IPC分类号： B01D61/44
CPC分类号： B01D67/0062 , B01D2311/2623
摘要： Provided is a microfluidic device for electrochemically regulating the pH of a fluid. The microfluidic device includes: an ion-exchange material; an anode chamber having a surface defined by a surface of the ion-exchange material and an anode electrode disposed along an edge of the surface of the anode chamber; and a cathode chamber having a surface defined by a surface of the ion-exchange material and a cathode electrode disposed along an edge of the surface of the cathode chamber, wherein the anode chamber and the cathode chamber are separated by an insulation material.
摘要翻译：提供了用于电化学调节流体pH的微流体装置。微流体装置包括：离子交换材料; 具有由所述离子交换材料的表面限定的表面的阳极室和沿着所述阳极室的表面的边缘设置的阳极电极; 以及阴极室，其具有由离子交换材料的表面限定的表面和沿着阴极室的表面的边缘设置的阴极电极，其中阳极室和阴极室由绝缘材料分隔开。

64. 发明申请

US20060266650A1 Apparatus for regulating salt concentration using electrodialysis, lab-on-a-chip including the same, and method of regulating salt concentration using the apparatus 有权
标题翻译：使用电渗析法调节盐浓度的装置，包括该盐浓度的实验室的方法以及使用该装置调节盐浓度的方法
公开(公告)号：US20060266650A1
公开(公告)日：2006-11-30
申请号：US11440872
申请日：2006-05-25
申请人： Jung-im Han , Young-sun Lee , Joon-ho Kim
发明人： Jung-im Han , Young-sun Lee , Joon-ho Kim
IPC分类号： B01D61/42
CPC分类号： B01D61/422 , B01D61/46 , B01L3/5027 , G01N2001/4011
摘要： An apparatus for regulating salt concentration, a lab-on-a-chip including the same and a method of regulating salt concentration using the apparatus are provided. The apparatus includes: a reaction chamber that is defined by a cation exchange membrane and an anion exchange membrane and is selected from the group consisting of a biomolecule extraction chamber, an amplification chamber, a hybridization chamber, and a detection chamber; a first electrode chamber that is defined by the anion exchange membrane and a first electrode and includes an ion exchange medium; and a second electrode chamber that is defined by the cation exchange membrane and a second electrode and includes an ion exchange medium. Even without injecting solutions with different salt concentrations into the reaction chamber by operating pumps and valves for each operation stage, the salt concentration can be reversibly regulated in situ by adjusting the polarity, intensity and application time of a voltage.
摘要翻译：提供了一种调节盐浓度的装置，包括该盐浓度的实验室，以及使用该装置调节盐浓度的方法。该装置包括：由阳离子交换膜和阴离子交换膜限定的反应室，其选自生物分子提取室，扩增室，杂交室和检测室; 由阴离子交换膜和第一电极限定并包括离子交换介质的第一电极室; 以及由阳离子交换膜和第二电极限定并包括离子交换介质的第二电极室。即使在每个操作阶段通过操作泵和阀都不会将具有不同盐浓度的溶液注入反应室中，可以通过调节电压的极性，强度和施加时间来原位调节盐浓度。

65. 发明申请

US20060188906A1 Microfluidic chip for multiple bioassay and method of manufacturing the same 有权
标题翻译：微流控芯片用于多种生物测定及其制造方法
公开(公告)号：US20060188906A1
公开(公告)日：2006-08-24
申请号：US11335939
申请日：2006-01-20
申请人： Joon-ho Kim , Jun-hong Min , Kyung-hwan Park , Soo-suk Lee , Hyun-gyu Park
发明人： Joon-ho Kim , Jun-hong Min , Kyung-hwan Park , Soo-suk Lee , Hyun-gyu Park
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： B01L3/502707 , B01J2219/00522 , B01J2219/00527 , B01J2219/00585 , B01J2219/00605 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00632 , B01J2219/00644 , B01J2219/00657 , B01J2219/00659 , B01J2219/00711 , B01J2219/0072 , B01L3/5025 , B01L2200/12 , B01L2300/0636 , B01L2300/0816 , B01L2300/0887 , B82Y30/00
摘要： Provided are a microfluidic chip for multiple bioassay and a method of manufacturing the same. The method includes: forming microfluidic channels by coupling a channel structure having grooves for sample channels and probe channels in a bottom surface and a substrate; forming probe immobilization regions at intersections between the probe channels and the sample channels; and forming blocking walls in the probe channels before and after each of the probe immobilization regions to prevent mixing of target sample. Since the probes are immobilized in the microfluidic channels after the formation of the microfluidic channels, difficulty in connecting fluidic channels after the immobilization of probes on a substrate can be removed. In addition, a microfluidic platform for a multiple bioassay into which a plurality of samples can be simultaneously loaded can be formed.
摘要翻译：提供了用于多重生物测定的微流控芯片及其制造方法。该方法包括：通过在底表面和基底中耦合具有用于样品通道和探针通道的沟槽的通道结构来形成微流体通道; 在探针通道和样品通道之间的交叉处形成探针固定区域; 并且在每个探针固定区域之前和之后在探针通道中形成阻挡壁以防止靶样品混合。由于在形成微流体通道之后将探针固定在微流体通道中，因此可以除去在探针固定在基底上之后连接流体通道的困难。此外，可以形成用于多个生物测定的微流体平台，多个样品可以同时装载到该微生物测定中。

66. 发明申请

US20060177874A1 Sensing switch and detecting method using the same 失效
公开(公告)号：US20060177874A1
公开(公告)日：2006-08-10
申请号：US11347185
申请日：2006-02-03
申请人： Kyu-tae Yoo , Joon-ho Kim , Jun-hong Min , Sung-ouk Jung , Ji-na Namgoong , Kui-hyun Kim , Jeo-young Shim
发明人： Kyu-tae Yoo , Joon-ho Kim , Jun-hong Min , Sung-ouk Jung , Ji-na Namgoong , Kui-hyun Kim , Jeo-young Shim
IPC分类号： G01N33/53 , C12M1/34
CPC分类号： G01N33/54373
摘要： Provided are a sensing switch and a sensing method using the same. The sensing switch includes: a substrate; a supporter on the substrate; a sensing plate that is connected to a side of the supporter and is in parallel with the substrate by a predetermined distance; a receptor binding region on an upper surface of an end portion of the sensing plate; an electric or magnetic field generation device that induces deflection of the sensing plate when a receptor bound to the receptor binding region is selectively bound to an electrically or magnetically active ligand; and a pair of switching electrodes that are separated by a predetermined distance and is connected when the sensing plate contacts the substrate due to the deflection of the sensing plate. A target material need not be labelled, a signal processing of a fluorescent or electrical detection signal using an analysis apparatus is not required, and a signal can be directly decoded by confirming whether a current flows through the switch.

67. 发明申请

US20050202489A1 Method and apparatus for amplifying nucleic acids 有权
标题翻译：扩增核酸的方法和装置
公开(公告)号：US20050202489A1
公开(公告)日：2005-09-15
申请号：US11071383
申请日：2005-03-03
申请人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
发明人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
IPC分类号： C12N15/09 , B01F5/06 , B01F13/00 , B01F15/02 , B01L3/00 , B01L7/00 , C12M1/00 , C12M1/38 , C12N15/00 , C12Q1/68 , G01N35/00 , G01N35/08 , C12P19/34
CPC分类号： G01N35/08 , B01F5/0646 , B01F5/0647 , B01F5/0654 , B01F13/0071 , B01F2015/0221 , B01J2219/00783 , B01J2219/00824 , B01J2219/00831 , B01J2219/00833 , B01J2219/00837 , B01J2219/0086 , B01J2219/00873 , B01J2219/00891 , B01L3/5027 , B01L3/502715 , B01L2300/0809 , B01L2300/0861 , B01L2300/0883 , G01N2035/00366 , G01N2035/00514
摘要： Provided are a method and apparatus for amplifying nucleic acids. The method includes introducing into a reaction vessel via different inlet channels a reactant aqueous solution containing reactants for nucleic acid amplification and a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction, creating a plurality of reactant aqueous solution droplets surrounded by the fluid by contacting the reactant aqueous solution with the fluid in the reaction vessel, and amplifying the nucleic acids in the reactant aqueous solution droplets. The apparatus includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate.
摘要翻译：提供了用于扩增核酸的方法和装置。该方法包括通过不同的入口通道将含有用于核酸扩增的反应物的反应物水溶液和与反应物水溶液相分离并且不参与扩增反应的流体引入反应容器，形成多个反应物水溶液通过使反应物水溶液与反应容器中的流体接触，并且扩增反应物水溶液液滴中的核酸，由流体包围的液滴。该装置包括基板，形成在基板内部的反应容器，形成在基板内部的至少一个第一入口通道，与反应容器的端部连接，并且允许将含有用于核酸扩增的反应物的反应物水溶液引入到反应容器，形成在基板内部的第二入口通道，连接到反应容器的端部，并且允许引入与反应物水溶液相分离并且不参与扩增反应的流体进入反应容器，以及加热单元，其以与基板热接触并加热基板的方式安装在基板上。

68. 发明授权

US09267618B2 Microvalve device and method of manufacturing the same 有权
标题翻译：微阀装置及其制造方法
公开(公告)号：US09267618B2
公开(公告)日：2016-02-23
申请号：US13110452
申请日：2011-05-18
申请人： Chin-sung Park , Won-jong Jung , Jae-young Kim , Joon-ho Kim
发明人： Chin-sung Park , Won-jong Jung , Jae-young Kim , Joon-ho Kim
IPC分类号： B01L99/00 , F16K99/00 , B01L3/00
CPC分类号： F16K99/0059 , B01L3/502707 , B01L3/502738 , B01L2300/0816 , B01L2300/0887 , B01L2300/123 , B01L2400/0638 , F16K99/0001 , F16K99/0015 , F16K2099/008 , F16K2099/0084
摘要： A microvalve device comprises: a first substrate having a first surface, where at least one fluidic channel is formed into the first surface of the first substrate; and at least one valve seat is formed in the fluidic channel; a second substrate having a second surface, the second substrate comprising at least one air channel and at least one air chamber formed on the second surface of the second substrate, where the air chamber is connected to the air channel; and an elastic film interposed between the first substrate and the second substrate, where an upper portion of the valve seat is lower than the first surface of the first substrate.
摘要翻译：微阀装置包括：具有第一表面的第一基板，其中至少一个流体通道形成在第一基板的第一表面中; 并且至少一个阀座形成在流体通道中; 具有第二表面的第二基板，所述第二基板包括至少一个空气通道和形成在所述第二基板的第二表面上的至少一个空气室，其中所述空气室连接到所述空气通道; 以及插入在第一基板和第二基板之间的弹性膜，其中阀座的上部比第一基板的第一表面低。

69. 发明授权

US09000399B2 Fluorescence detecting optical system and multi-channel fluorescence detection apparatus including the same 有权
标题翻译：荧光检测光学系统和包括其的多通道荧光检测装置
公开(公告)号：US09000399B2
公开(公告)日：2015-04-07
申请号：US13271101
申请日：2011-10-11
申请人： Kyung-ho Kim , Joon-ho Kim , Kak Namkoong , Won-seok Chung
发明人： Kyung-ho Kim , Joon-ho Kim , Kak Namkoong , Won-seok Chung
IPC分类号： G01N21/64 , G01J3/44 , G01J3/02 , G01N21/05 , G01N21/03
CPC分类号： G01N21/645 , G01J3/0208 , G01J3/0229 , G01J3/0237 , G01J3/4406 , G01N21/05 , G01N2021/0346 , G01N2021/6419 , G01N2021/6421 , G01N2021/6478 , G01N2201/0635
摘要： A fluorescence detection optical system detects fluorescence beams with two or more different wavelengths and maintains a focal position through an automatic focusing function. A multi-channel fluorescence detection apparatus includes the fluorescence detection optical system. The fluorescence detection optical system includes an automatic focusing unit which receives light reflected off a microfluidic device and determines a focal point by using an astigmatic method or a knife edge method, and an actuator which adjusts a position of an objective lens according to control of the automatic focusing unit. In addition, the fluorescence detection optical system may include a plurality of dual band pass filters, dichroic devices, etc., which provide light beams emitted from at least two light sources and transfer fluorescence generated from the microfluidic device to a photodetector.
摘要翻译：荧光检测光学系统检测具有两个或更多个不同波长的荧光束并通过自动聚焦功能保持焦点位置。多通道荧光检测装置包括荧光检测光学系统。荧光检测光学系统包括自动聚焦单元，其接收从微流体装置反射的光并且通过使用散光方法或刀刃方法确定焦点，以及致动器，其根据控制的方式调整物镜的位置自动对焦单元此外，荧光检测光学系统可以包括多个双带通滤波器，二向色装置等，其提供从至少两个光源发射的光束并将从微流体装置产生的荧光转移到光电检测器。

70. 发明授权

US08030479B2 Method of separating small RNA molecules using kosmotropic salt 有权
标题翻译：使用去离子水分离小RNA分子的方法
公开(公告)号：US08030479B2
公开(公告)日：2011-10-04
申请号：US12110373
申请日：2008-04-28
申请人： Myo-yong Lee , Nam Huh , Joon-ho Kim
发明人： Myo-yong Lee , Nam Huh , Joon-ho Kim
IPC分类号： C07H21/00
CPC分类号： C12N15/1006
摘要： Disclosed is a method of separating small RNAs of 200 nucleotides or less from larger RNAs on a solid support, using a kosmotropic salt of different concentrations.
摘要翻译：公开了一种使用不同浓度的可变性盐分离从固体支持物上的较大RNA分离200个核苷酸或更少的小RNA的方法。

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