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61. 发明申请

US20090137012A1 Process for producing lactonase and utilitzation thereof 审中-公开
标题翻译：生产内酯酶的方法及其功效
公开(公告)号：US20090137012A1
公开(公告)日：2009-05-28
申请号：US12232870
申请日：2008-09-25
申请人： Sakayu Shimizu , Michihiko Kataoka , Keiji Sakamoto
发明人： Sakayu Shimizu , Michihiko Kataoka , Keiji Sakamoto
IPC分类号： C12P17/04
CPC分类号： C12P41/003 , C12N9/18 , C12P17/04
摘要： It has been required to economically produce a large amount of optically active γ-lactone derivatives (for example, pantolactone), which are useful as intermediates in synthesizing useful substances such as pharmaceutical drugs. To achieve this object, it is advantageous to employ an enzymatic technique of asymmetric hydrolysis with a hydrolyzing enzyme lactonase. However, it remains troublesome to prepare the enzyme or utilize a microorganism capable of producing the enzyme. Also, it is difficult to obtain a sufficient and stable enzymatic activity in the case of using recombination techniques. In producing lactonase, which asymmetrically hydrolyzes a γ-lactone derivative such as racemic pantolactone selectively, by a recombination technique, both mature lactonase DNA and signal peptide region DNA are transferred into a host. Thus, a stable lactonase activity comparable to naturally-occurring one can be achieved and a transformant sustaining a high enzyme activity in a stable manner can be acquired, thereby allowing the efficient and industrially advantageous asymmetric synthesis of γ-lactone derivatives.
摘要翻译：需要经济地生产大量的用作合成有用物质如药物的中间体的光学活性γ-内酯衍生物（例如泛酸内酯）。为了达到这个目的，使用水解酶内酯酶的不对称水解酶技术是有利的。然而，制备酶或利用能够产生酶的微生物仍然是麻烦的。此外，在使用重组技术的情况下，难以获得足够稳定的酶活性。在制备内含子选择性地水解γ-内酯衍生物如外消旋泛酸内酯的内切酶中，通过重组技术，将成熟内含酶DNA和信号肽区DNA两者转移到宿主中。因此，可以获得与天然存在的活性相当的稳定的内含酶活性，并且可以获得以稳定的方式维持高酶活性的转化体，从而允许有效和工业上有利的γ-内酯衍生物的不对称合成。

62. 发明授权

US07202068B2 Enone reductase and methods of making and using thereof 有权
标题翻译： Enone还原酶及其制备和使用方法
公开(公告)号：US07202068B2
公开(公告)日：2007-04-10
申请号：US10505396
申请日：2003-02-14
申请人： Michihiko Kataoka , Sakayu Shimizu
发明人： Michihiko Kataoka , Sakayu Shimizu
IPC分类号： C12N9/02
CPC分类号： C12N9/001 , C12P7/26
摘要： An isolated enone reductase characterized by a molecular mass of 61300+/−5000 Da; NADPH and NADPH as co-factor; a temperature optimum of 55–60° C. at pH 7.4; a pH optimum of 4.5–8.5 and substrate specificity on ∝,β-unsatured ketones; a process for producing it from a microorganism and a process for producing levodione from ketoisophorone using such reductase.
摘要翻译：分离的烯酮还原酶，其特征在于分子量为61300 +/- 5000Da; NADPH和NADPH作为辅因子; 温度最适为55-60℃，pH 7.4; pH最适为4.5-8.5，底物特异性为α，β-不熟酮; 从微生物生产它的方法和使用这种还原酶从酮异二酮生产左旋二酮的方法。

63. 发明申请

US20060128000A1 Enone reductase gene and microbial production of levodione 失效
公开(公告)号：US20060128000A1
公开(公告)日：2006-06-15
申请号：US10528960
申请日：2003-09-19
申请人： Michihiko Kataoka , Sakayu Shimizu
发明人： Michihiko Kataoka , Sakayu Shimizu
IPC分类号： C12P7/26 , C07H21/04 , C12N9/02 , C12N15/74 , C12N1/21
CPC分类号： C12N9/001 , C12P7/26
摘要： Disclosed is an isolated DNA comprising a nucleotide sequence coding for an enzyme having enone reductase activity wherein the enzyme is characterized by the following physico-chemical properties: (a) molecular mass: 61,300±5,000 Da (estimated using gel filtration, consisting of one subunit); (b) co-factor: NADPH and NADH; (c) substrate specificity: active on α,β-unsaturated ketons; (d) optimum temperature: 55-60° C. at pH 7.4; and (e) optimum pH: pH 4.5-8.5.

64. 发明申请

US20060073578A1 Process for producing unsaturated fatty acid-containing oils 有权
标题翻译：用于生产不饱和脂肪酸的油的方法
公开(公告)号：US20060073578A1
公开(公告)日：2006-04-06
申请号：US11287369
申请日：2005-11-28
申请人： Kenichi Higashiyama , Kengo Akimoto , Sakayu Shimizu
发明人： Kenichi Higashiyama , Kengo Akimoto , Sakayu Shimizu
IPC分类号： C12P7/64 , C12N1/20
CPC分类号： C12P7/6472 , A23D9/00 , A23K20/158 , A23L33/12 , A23L33/40 , A23V2002/00 , C12P7/64 , Y10S426/801 , Y10S426/807 , A23V2250/54252 , A23V2250/1874 , A23V2250/1868 , A23V2250/1862 , A23V2250/187 , A23V2250/612 , A23V2250/28 , A23V2250/70 , A23V2250/156
摘要： An edible oil obtained by culturing a microorganism belong to the genus Mortierella subgenus Mortierella in a medium containing a nitrogen source derived from soybean is discussed. The oils obtained have a low 24,25-methylenecholest-5-en-3β-ol content.
摘要翻译：通过在含有源自大豆的氮源的培养基中培养微生物而获得的食用油属于被孢霉属（Mortierella subgenus Mortidalla）属。得到的油具有低的24,25-亚甲基胆甾-5-烯-3β-醇含量。

65. 发明授权

US06534297B2 Recombinantly producing levodione reductase 失效
公开(公告)号：US06534297B2
公开(公告)日：2003-03-18
申请号：US09773748
申请日：2001-01-31
申请人： Sakayu Shimizu , Masaru Wada
发明人： Sakayu Shimizu , Masaru Wada
IPC分类号： C12N902
CPC分类号： C12N9/0008 , C12P7/26
摘要： The present invention is directed to genetic material useful for the preparation of actinol, such as an isolated DNA including a nucleotide sequence coding for an enzyme having levodione reductase activity, a polypeptide encoded by such a DNA, recombinant organisms, and the like. These genetic materials may originate from Corynebacterium, Cellulomonas, Planococcus, Arthrobacter, and the like. The present invention also provides a process for the production of actinol.

66. 发明授权

US06448052B2 Carbonyl reductase enzyme and methods for its use 有权
标题翻译：羰基还原酶及其使用方法
公开(公告)号：US06448052B2
公开(公告)日：2002-09-10
申请号：US09777157
申请日：2001-02-05
申请人： Yoshihiko Yasohara , Noriyuki Kizaki , Junzo Hasegawa , Masaru Wada , Sakayu Shimizu , Michihiko Kataoka , Kazuhiko Yamamoto , Hiroshi Kawabata , Keiko Kita
发明人： Yoshihiko Yasohara , Noriyuki Kizaki , Junzo Hasegawa , Masaru Wada , Sakayu Shimizu , Michihiko Kataoka , Kazuhiko Yamamoto , Hiroshi Kawabata , Keiko Kita
IPC分类号： C12P762
CPC分类号： C12P7/62 , C12N9/0006 , C12P41/002
摘要： An enzyme having carbonyl reduction activity of reducing a carbonyl compound asymmetrically to produce an optically active alcohol, a DNA coding the enzyme, a plasmid having the DNA, a transformant which is a cell transformed with the plasmid, and a production method of an optically active alcohol using the enzyme and/or the transformed cell are provided.
摘要翻译：具有不对称还原羰基化合物以产生光学活性醇的羰基还原活性的酶，编码该酶的DNA，具有该DNA的质粒，作为用该质粒转化的细胞的转化体的转化体和光学活性物质的制备方法提供使用酶和/或转化细胞的醇。

67. 发明授权

US07615364B2 Polypeptide having activity of unsaturating W3-fatty acid, polynucleotide coding for the polypeptide and use thereof 有权
标题翻译：具有不饱和的W3-脂肪酸活性的多肽，编码多肽的多核苷酸及其用途
公开(公告)号：US07615364B2
公开(公告)日：2009-11-10
申请号：US11660093
申请日：2005-08-19
申请人： Sakayu Shimizu , Eiji Sakuradani
发明人： Sakayu Shimizu , Eiji Sakuradani
IPC分类号： C12N9/02 , C12N15/00 , C12N1/20 , C07H21/02 , C12N5/00 , A01H9/00
CPC分类号： C12P7/6427 , C12N9/0083 , C12P7/6409
摘要： The present invention provides a polypeptide having a wide substrate specificity and having a ω3 fatty acid desaturation activity, which makes efficiently unsaturated bond at ω3 position, and a polynucleotide coding for the same. By expressing the polypeptide in the organism, mass production of n-3 series PUFAs is enabled. Specifically, the polypeptide having a ω3 fatty acid desaturation activity and consisting of the amino acid sequence represented by SEQ ID NO: 1, a polynucleotide coding for a polypeptide having a ω3 fatty acid desaturation activity that consists of the base sequence represented by SEQ ID NO: 2 or SEQ ID NO: 3, and the like is useful for production of n-3 series fatty acids.
摘要翻译：本发明提供了具有宽底物特异性并具有ω3脂肪酸去饱和活性的多肽，其在ω3位上有效地形成不饱和键，以及编码该多肽的多核苷酸。通过在生物体中表达多肽，可以批量生产n-3系PUFA。具体地说，具有ω3脂肪酸去饱和活性的多肽，由SEQ ID NO：1表示的氨基酸序列组成，该多核苷酸编码具有ω3脂肪酸去饱和活性的多肽，其由SEQ ID NO：：2或SEQ ID NO：3等可用于生产n-3系列脂肪酸。

68. 发明授权

US07514244B2 Lipid and food compositions containing docosahexaenoic acid and docosapentaenoic acid 失效
标题翻译：含有二十二碳六烯酸和二十二碳五烯酸的脂质和食物组合物
公开(公告)号：US07514244B2
公开(公告)日：2009-04-07
申请号：US10341300
申请日：2003-01-13
申请人： Satohiro Tanaka , Toshiaki Yaguchi , Sakayu Shimizu , Tsutomu Sogo , Shigeaki Fujikawa
发明人： Satohiro Tanaka , Toshiaki Yaguchi , Sakayu Shimizu , Tsutomu Sogo , Shigeaki Fujikawa
IPC分类号： C12P7/64 , A23K1/16
CPC分类号： C12P7/6427 , A23D9/007 , A23K20/158 , A23L33/12 , C11C1/045 , C12P7/6472 , C12R1/645 , C12R1/89
摘要： A process for preparing lipids which contain docosahexaenoic acid (DHA) and/or docosapentaenoic acid (DPA) is disclosed. The process includes the steps of cultivating in a medium a microorganism which belongs to the genus Ulkenia having the ability to produce DHA and/or DPA and recovering the lipids from the culture. The process may further include the step of separating DHA and/or DPA from the lipids.
摘要翻译：公开了一种制备含有二十二碳六烯酸（DHA）和/或二十二碳五烯酸（DPA）的脂质的方法。该方法包括在培养基中培养属于具有产生DHA和/或DPA的能力的乌尔肯尼亚属的微生物并从培养物中回收脂质的步骤。该方法还可以包括从脂质中分离DHA和/或DPA的步骤。

69. 发明申请

US20080269329A1 Process for Production of Microbial Fat/Oil Containing Discretional Amount of Diacylglycerol and Said Fat/Oil 审中-公开
标题翻译：含有二酰基甘油和所述脂肪/油的离散量的微生物脂肪/油的生产方法
公开(公告)号：US20080269329A1
公开(公告)日：2008-10-30
申请号：US11660859
申请日：2005-08-19
申请人： Shigeaki Fujikawa , Eiji Sakuradani , Jun Ogawa , Sakayu Shimizu
发明人： Shigeaki Fujikawa , Eiji Sakuradani , Jun Ogawa , Sakayu Shimizu
IPC分类号： C12P7/64 , A61K31/201 , C07C57/00 , A61K31/202
CPC分类号： A61K8/375 , A23D9/013 , A23K20/158 , A23L33/115 , A23L33/12 , A23V2002/00 , A61K8/67 , A61K8/922 , A61K8/99 , A61Q19/00 , C11B1/10 , C11B3/12 , C12P7/6472 , A23V2250/182 , A23V2250/1882
摘要： In a process for the production of diacylglycerol-containing fat/oil where the ratio of diacylglycerol to the total neutral lipid is more than 20% by weight and a polyunsaturated fatty acid is a constituting fatty acid, a process which is characterized in that a microbe being able to produce said fat/oil is incubated and, if desired, said fat/oil is collected.
摘要翻译：在制备含二酰基甘油的脂肪/油的方法中，其中二酰基甘油与总中性脂质的比例大于20重量％，多不饱和脂肪酸是构成脂肪酸的方法，其特征在于将微生物能够产生所述脂肪/油的温育，如果需要，收集所述脂肪/油。

70. 发明申请

US20080032335A1 Polypeptide Having Activity of Unsaturating W3-Fatty Acid, Polynucleotide Coding for the Polypeptide and Use Thereof 有权
标题翻译：具有不饱和W3-脂肪酸活性的多肽，用于多肽的多核苷酸编码及其用途
公开(公告)号：US20080032335A1
公开(公告)日：2008-02-07
申请号：US11660093
申请日：2005-08-19
申请人： Sakayu Shimizu , Eiji Sakuradani
发明人： Sakayu Shimizu , Eiji Sakuradani
IPC分类号： C12N15/09 , A23L1/30 , C07H21/04 , C07K14/37 , C12N5/10 , C12P7/64 , C12P21/00 , C12N15/63 , C07K16/14 , C12N1/15
CPC分类号： C12P7/6427 , C12N9/0083 , C12P7/6409
摘要： The present invention provides a polypeptide having a wide substrate specificity and having a ω3 fatty acid desaturation activity, which makes efficiently unsaturated bond at ω3 position, and a polynucleotide coding for the same. By expressing the polypeptide in the organism, mass production of n-3 series PUFAs is enabled. Specifically, the polypeptide having a ω3 fatty acid desaturation activity and consisting of the amino acid sequence represented by SEQ ID NO: 1, a polynucleotide coding for a polypeptide having a ω3 fatty acid desaturation activity that consists of the base sequence represented by SEQ ID NO: 2 or SEQ ID NO: 3, and the like is useful for production of n-3 series fatty acids.
摘要翻译：本发明提供了具有宽底物特异性并具有ω3脂肪酸去饱和活性的多肽，其在ω3位上有效地形成不饱和键，以及编码该多肽的多核苷酸。通过在生物体中表达多肽，可以批量生产n-3系PUFA。具体地说，具有ω3脂肪酸去饱和活性的多肽，由SEQ ID NO：1表示的氨基酸序列组成，该多核苷酸编码具有ω3脂肪酸去饱和活性的多肽，其由SEQ ID NO：：2或SEQ ID NO：3等可用于生产n-3系列脂肪酸。

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