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51. 发明授权

US06743611B2 Expression systems utilizing autolyzing fusion proteins and a novel reducing polypeptide 失效
标题翻译：使用自溶融合蛋白和新型还原多肽的表达系统
公开(公告)号：US06743611B2
公开(公告)日：2004-06-01
申请号：US09842347
申请日：2001-04-25
申请人： Tohru Takahashi , Nobufusa Serizawa , Ryuta Koishi , Ichiro Kawashima
发明人： Tohru Takahashi , Nobufusa Serizawa , Ryuta Koishi , Ichiro Kawashima
IPC分类号： C12N902
CPC分类号： C12N9/0051 , A61K38/00 , C07K14/5431 , C07K2319/00 , C07K2319/35 , C07K2319/40 , C07K2319/50 , C07K2319/735 , C07K2319/75 , C07K2319/95 , C12N9/506 , C12N15/62
摘要： The present invention provides expression systems for exogenous polypeptides wherein the polypeptide is expressed as a fusion protein together with clover yellow virus Nuclear Inclusion a (NIa), the NIa component serving to autolyze the fusion protein after expression. This system can be used to express a novel polypeptide which we have designated KM31-7 protein and which is capable of reducing dichloroindophenol and reduced glutathione. This polypeptide is useful in the treatment of disorders caused by oxidative stress.
摘要翻译：本发明提供用于外源多肽的表达系统，其中多肽与三叶草黄病毒核包涵a（NIa）一起表达为融合蛋白，其用于在表达后自动融合融合蛋白。该系统可用于表达我们已经命名为KM31-7蛋白并能够还原二氯靛酚和还原型谷胱甘肽的新型多肽。该多肽可用于治疗由氧化应激引起的疾病。

52. 发明授权

US06737245B1 Luciferase expression cassettes and methods of use 有权
标题翻译：荧光素酶表达盒和使用方法
公开(公告)号：US06737245B1
公开(公告)日：2004-05-18
申请号：US09657289
申请日：2000-09-07
申请人： Kevin P. Francis , Pamela R. Contag , Danny J. Joh
发明人： Kevin P. Francis , Pamela R. Contag , Danny J. Joh
IPC分类号： C12N902
CPC分类号： G01N33/52 , C12N15/74
摘要： The present invention relates to bacterial luciferase expression cassettes suitable for conferring bioluminescence properties on Gram-positive bacteria, cells transformed with such cassettes, and methods of making and using such cassettes.
摘要翻译：本发明涉及适用于赋予革兰氏阳性菌的细菌荧光素酶表达盒，用这种盒转化的细胞，以及制备和使用这些盒的方法。

53. 发明授权

US06703229B2 Aryl propenal double bond reductase 有权
标题翻译：芳基丙烯双键还原酶
公开(公告)号：US06703229B2
公开(公告)日：2004-03-09
申请号：US09820096
申请日：2001-03-27
申请人： Hiroyuki Kasahara , Laurence B. Davin , Norman G. Lewis
发明人： Hiroyuki Kasahara , Laurence B. Davin , Norman G. Lewis
IPC分类号： C12N902
CPC分类号： C12N9/001
摘要： In one aspect, the present invention provides isolated nucleic acid molecules that each: (a) encode an aryl propenal double bond reductase; and (b) hybridize to a nucleic acid molecule consisting of the complement of the nucleic acid sequence set forth in SEQ ID NO:1 under defined conditions. The present invention also provides isolated aryl propenal double bond reductases. In other aspects, the present invention provides methods of enhancing or inhibiting the expression of aryl propenal double bond reductases in a plant.
摘要翻译：一方面，本发明提供分离的核酸分子，其各自：（a）编码芳基丙烯醛双键还原酶; 和（b）在确定的条件下与由SEQ ID NO：1所示的核酸序列的互补体组成的核酸分子杂交。本发明还提供了分离的芳基丙烯醛双键还原酶。在其它方面，本发明提供增强或抑制植物中芳基丙烯醛双键还原酶表达的方法。

54. 发明授权

US06610520B1 Gene encoding human manganese superoxide dismutase and recombinant polypeptide encoded thereby 失效
标题翻译：编码人类锰超氧化物歧化酶的基因和由此编码的重组多肽
公开(公告)号：US06610520B1
公开(公告)日：2003-08-26
申请号：US08299047
申请日：1994-08-31
申请人： Jacob R. Hartman , Yaffa Beck
发明人： Jacob R. Hartman , Yaffa Beck
IPC分类号： C12N902
CPC分类号： A61K38/446 , C12N9/0089 , Y02A50/473
摘要： A double-stranded cDNA molecule which includes DNA encoding human manganese superoxide dismutase has been created. The sequence of one strand of a double-stranded DNA molecule which encodes human manganese superoxide dismutase has been discovered. Such molecules may be introduced in procaryotic, e.g., bacterial, or eukaryotic, e.g., yeast or mammalian, cells and the resulting cells cultured or grown under suitable conditions so as to produce human manganese superoxide dismutase or analogs thereof which may then be recovered. By this invention, human MnSOD gene fragments from various plasmids may be ligated to yield a complete genomic human MnSOD gene fragment. Human MnSOD or analogs thereof may be used to catalyze the reduction of superoxide radicals, reduce reperfusion injury, prolong the survival time of isolated organs, or treat inflammations. The invention also concerns a method of producing enzymatically active human manganese superoxide dismutase or an analog thereof in a bacterial cell which contains and is capable of expressing a DNA sequence encoding the superoxide dismutase by maintaining the bacterial cell under suitable conditions and in a suitable production medium. The production medium is supplemented with an amount of Mn++ so that the concentration of Mn++ in the medium is greater than about 2 ppm. Genomic MnSOD DNA should also be capable of expression in eucaryotic cells under suitable conditions. This invention also concerns a method of recovering purified enzymatically active manganese superoxide dismutase from bacterial cells. It should also be possible to recover manganese SOD from genomic MnSOD DNA expressed in eucaryotic cells using similar methods.
摘要翻译：已经产生了包含编码人锰超氧化物歧化酶的DNA的双链cDNA分子。已经发现编码人锰超氧化物歧化酶的双链DNA分子的一条链的序列。这样的分子可以在原核细胞中，例如细菌的，或真核的，例如酵母或哺乳动物细胞中引入，所得细胞在合适的条件下培养或生长，以产生可以被回收的人锰超氧化物歧化酶或其类似物。通过本发明，可以连接各种质粒的人类MnSOD基因片段以产生完整的基因组人类MnSOD基因片段。人类MnSOD或其类似物可用于催化超氧自由基的还原，减少再灌注损伤，延长分离的器官的存活时间或治疗炎症。本发明还涉及生产酶活性人锰超氧化物歧化酶或其类似物的方法在细菌细胞中，其含有并且能够通过将细菌细胞维持在合适的条件下并在合适的生产培养基中表达编码超氧化物歧化酶的DNA序列。生产培养基补充一定量的Mn ++，使得培养基中Mn ++的浓度大于约2ppm。基因组MnSOD DNA也应该能够在合适条件下在真核细胞中表达。本发明还涉及从细菌细胞中回收纯化的酶活性锰超氧化物歧化酶的方法。也可以使用类似的方法从真核细胞中表达的基因组MnSOD DNA中回收锰SOD。

55. 发明授权

US06599725B2 Polypeptide compositions for controlling cell death and disease resistance in plants 有权
标题翻译：用于控制植物中细胞死亡和抗病性的多肽组合物
公开(公告)号：US06599725B2
公开(公告)日：2003-07-29
申请号：US09776490
申请日：2001-02-02
申请人： Steven P. Briggs , Gurmukh S. Johal , John Gray
发明人： Steven P. Briggs , Gurmukh S. Johal , John Gray
IPC分类号： C12N902
CPC分类号： C12N9/0071 , C12N15/8207 , C12N15/8282 , C12N15/8289
摘要： The present invention is drawn to methods and compositions for suppressing cell death in plants. Specifically, novel proteins and genes are provided for use in plant transformation. The proteins and genes are useful for activating disease resistance, enhancing plant cell transformation efficiency, engineering herbicide resistance, genetically targeting cell ablations, and other methods involving the regulation of cell death in plants.
摘要翻译：本发明涉及抑制植物细胞死亡的方法和组合物。具体地说，提供新的蛋白质和基因用于植物转化。蛋白质和基因可用于激活疾病抗性，增强植物细胞转化效率，工程除草剂抗性，遗传靶向细胞消融以及涉及调节植物细胞死亡的其它方法。

56. 发明授权

US06566108B1 Expression of functional cytochrome P450 monooxygenase system in enterobacteria 有权
标题翻译：功能性细胞色素P450单加氧酶体系在肠细菌中的表达
公开(公告)号：US06566108B1
公开(公告)日：2003-05-20
申请号：US09230233
申请日：1999-05-17
申请人： Charles Roland Wolf , Thomas Herbert Friedberg , Michael Patrick Pritchard
发明人： Charles Roland Wolf , Thomas Herbert Friedberg , Michael Patrick Pritchard
IPC分类号： C12N902
CPC分类号： C12N9/0042 , A61K38/00 , C07K2319/02 , C12N9/0077
摘要： A bacterial cell containing a functional cytochrome P450 monooxygenase system, said cell comprising a genetic construct capable of expressing a cytochrome P450 and genetic construct capable of expressing, separately from said cytochrome P450, a cytochrome P450 reductase, wherein each of the cytochrome P450 and the cytochrome P450 reductase have at their N-terminus a bacterial signal peptide. The cytochrome P450 and the cytochrome P450 reductase may be encoded by different constructs or the same construct. A bacterial cell containing a cytochrome P450 comprising a genetic construct encoding, and capable of expressing, said cytochrome P450 wherein the cytochrome P450 has, at is N-terminal, a bacterial signal peptide. The bacterial cells are useful as, for example, bioreactors, in drug testing and mutagenicity testing and as a source of cytochrome P450.
摘要翻译：一种含有功能性细胞色素P450单加氧酶系统的细菌细胞，所述细胞包含能够表达细胞色素P450的遗传构建体和能够与所述细胞色素P450分开表达细胞色素P450还原酶的遗传构建体，其中细胞色素P450和细胞色素 P450还原酶在其N末端具有细菌信号肽。细胞色素P450和细胞色素P450还原酶可以由不同的构建体或相同的构建体编码。含有细胞色素P450的细菌细胞，其包含编码并能够表达所述细胞色素P450的遗传构建体，其中细胞色素P450具有细菌信号肽，为N末端。细菌细胞可用作例如生物反应器，在药物测试和致突变性测试中以及作为细胞色素P450的来源。

57. 发明授权

US06534297B2 Recombinantly producing levodione reductase 失效
公开(公告)号：US06534297B2
公开(公告)日：2003-03-18
申请号：US09773748
申请日：2001-01-31
申请人： Sakayu Shimizu , Masaru Wada
发明人： Sakayu Shimizu , Masaru Wada
IPC分类号： C12N902
CPC分类号： C12N9/0008 , C12P7/26
摘要： The present invention is directed to genetic material useful for the preparation of actinol, such as an isolated DNA including a nucleotide sequence coding for an enzyme having levodione reductase activity, a polypeptide encoded by such a DNA, recombinant organisms, and the like. These genetic materials may originate from Corynebacterium, Cellulomonas, Planococcus, Arthrobacter, and the like. The present invention also provides a process for the production of actinol.

58. 发明授权

US06521433B1 cDNA sequences from plants that encode activities associated with isoflavone biosynthesis 失效
标题翻译：编码与异黄酮生物合成相关活性的植物的cDNA序列
公开(公告)号：US06521433B1
公开(公告)日：2003-02-18
申请号：US08931668
申请日：1997-09-17
申请人： Gary Michael Fader
发明人： Gary Michael Fader
IPC分类号： C12N902
CPC分类号： C12N9/001 , C12N9/90 , C12N15/8242 , C12N15/8243
摘要： This invention relates to an isolated nucleic acid fragment encoding soybean enzymes that catalyze steps in biosynthesis of isoflavones, the enzyme a member selected from the group consisting of chalcone isomerase, isoflavone reductase and vestitone reductase. The invention also relates to the construction of chimeric genes encoding all or a substantial portion of the enzymes, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the enzyme in a transformed host cell.
摘要翻译：本发明涉及编码大豆酶的分离核酸片段，其催化异黄酮的生物合成步骤，该酶是选自查耳酮异构酶，异黄酮还原酶和胎膜还原酶的成员。本发明还涉及在正义或反义方向上编码全部或大部分酶的嵌合基因的构建，其中嵌合基因的表达导致在转化的宿主细胞中产生酶水平的改变。

59. 发明授权

US06509307B1 Detergent compositions comprising phenol oxidizing enzymes from fungi 失效
标题翻译：包含来自真菌的酚氧化酶的洗涤剂组合物
公开(公告)号：US06509307B1
公开(公告)日：2003-01-21
申请号：US09868839
申请日：2001-09-24
申请人： Elizabeth Ann Bodie , Sebastiaan van der Velden , Comelis Hendrikus de Vries , Huaming Wang
发明人： Elizabeth Ann Bodie , Sebastiaan van der Velden , Comelis Hendrikus de Vries , Huaming Wang
IPC分类号： C12N902
CPC分类号： C12N9/0004 , C11D3/38654 , Y10S435/911
摘要： Disclosed herein are detergent compositions comprising novel phenol oxidizing enzymes encoded by nucleic acid capable of hybridizing to the nucleic acid having the sequence as shown in SEQ ID NO:1 and in particular those obtainable from fungus, in particular from Bipolaris spicifera, Curvularia pallescens and Amerosporium atrum. The present invention provides expression vectors and host cells comprising nucleic acid encoding phenol oxidizing enzymes, methods for producing the phenol oxidizing enzyme as well as methods for constructing expression osts.
摘要翻译：本文公开了洗涤剂组合物，其包含由能够与具有SEQ ID NO：1所示序列的核酸杂交的核酸编码的新型酚氧化酶，特别是可从真菌，特别是来自Bipolaris spicifera，Curvularia pallescens和Amerosporium atrum。本发明提供表达载体和包含编码酚氧化酶的核酸的宿主细胞，产生酚氧化酶的方法以及构建表达载体的方法。

60. 发明授权

US06420148B2 Method for cross-linking protein by using enzyme 失效
标题翻译：使用酶交联蛋白质的方法
公开(公告)号：US06420148B2
公开(公告)日：2002-07-16
申请号：US09576990
申请日：2000-05-24
申请人： Shotaro Yamaguchi
发明人： Shotaro Yamaguchi
IPC分类号： C12N902
CPC分类号： C12Y110/03002 , A23J3/06 , A23J3/10 , A23J3/12 , A23J3/225 , A23L13/48 , A23L13/65 , A23L17/65
摘要： A novel method for cross-linking a protein, which uses a multi-copper oxidase such as laccase, bilirubin oxidase, and the like.
摘要翻译：用于交联蛋白质的新方法，其使用多铜氧化酶如漆酶，胆红素氧化酶等。

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