专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

51. 发明公开

KR1020120101961A 단백질 나노입자를 이용한 3차원 나노구조체 有权
标题翻译：蛋白纳米颗粒的三维纳米结构域
公开(公告)号：KR1020120101961A
公开(公告)日：2012-09-17
申请号：KR1020110020130
申请日：2011-03-07
申请人： 고려대학교 산학협력단
发明人： 이지원 , 서혁성
IPC分类号： G01N33/68 , C12Q1/68 , G01N33/574 , B82Y15/00
CPC分类号： G01N33/68 , B82Y15/00 , C12Q1/68 , G01N33/683
摘要： PURPOSE: A three dimensional nanostructure using protein nanoparticles is provided to detect a target material. CONSTITUTION: A method for preparing a protein-DNA multilayer nanostructure comprises: a step of fixing a control protein of which net charge changes at a substrate according to pH concentration except for a metal nano dot; a step of changing pH concentration to adjust the net charge of the control protein; and a step of laminating a DNA layer and a protein layer on the metal nano dot. The protein layer contains allogenic or xenogenic protein particles. The substrate is formed of metal, nickel. [Reference numerals] (AA) Gold; (BB) Nickel layer; (CC) Si wafer; (DD) Adding (his)6-tagged proteasome alpha particles(pH 7.4, 8); (EE) Adding ssDNA(polyA, 20bp), conversion to pH 5; (FF) Adding DPS particles[first layer formation], conversion to pH 8; (GG) Adding dsDNA(519bp), conversion to pH 5; (HH) Adding DPS particles[second layer formation], moving to pH 8; (II) Adding dsDNA(519bp), conversion into pH 5; (JJ) Adding DPS particles[third layer formation], conversion to pH 8; (KK) Type 1 DPS particle layers; (LL) Type 2 DPS particle layers; (MM) N layer formation on fourth layer; (NN) Type 1 DPS particles for capturing antibody marker 1; (OO) Antigen peptide fixed on the surface, which specifically binds to antibody marker 1; (PP) Type 2 DPS particles for capturing antibody marker 2; (QQ) Antigen peptide fixed on the surface, which specifically binds to antibody marker 2; (RR) Simultaneous detection and diagnosis system(example)
摘要翻译：目的：提供使用蛋白质纳米颗粒的三维纳米结构以检测靶材料。构成：制备蛋白质-DNA多层纳米结构体的方法包括：除了金属纳米点之外，根据pH浓度固定基底上的净电荷变化的对照蛋白质的步骤; 改变pH浓度以调整对照蛋白的净电荷的步骤; 以及在金属纳米点上层叠DNA层和蛋白质层的步骤。蛋白质层含有异源或异种蛋白质颗粒。基板由金属，镍形成。（附图标记）（AA）金; （BB）镍层; （CC）Si晶片; （DD）添加（他的）6标记的蛋白酶体α粒子（pH 7.4,8）; （EE）加入ssDNA（polyA，20bp），转化为pH 5; （FF）添加DPS颗粒[第一层形成]，转化为pH8; （GG）加入dsDNA（519bp），转化为pH 5; （HH）添加DPS颗粒[第二层形成]，移至pH8; （II）加入dsDNA（519bp），转化为pH5; （JJ）添加DPS颗粒[第三层形成]，转化为pH8; （KK）1型DPS颗粒层; （LL）2型DPS颗粒层; （MM）N层形成在第四层上; （NN）用于捕获抗体标记物1的1型DPS颗粒; （OO）固定在表面上的抗原肽，其特异性结合抗体标记物1; （PP）用于捕获抗体标记物2的2型DPS颗粒; （QQ）固定在表面上的抗原肽，其特异性结合抗体标记物2; （RR）同步检测和诊断系统（示例）

52. 发明公开

US20230243840A1 An assay for measuring binding affinity for cardiolipin of biologically active compounds 审中-公开
公开(公告)号：US20230243840A1
公开(公告)日：2023-08-03
申请号：US18011916
申请日：2021-05-24
申请人： Latvian Institute of Organic Synthesis
发明人： Pavels Arsenjans , Pavels Dimitrijevs
IPC分类号： G01N33/58 , G01N33/92 , G01N33/68
CPC分类号： G01N33/582 , G01N33/92 , G01N33/683 , G01N2405/04
摘要： The present invention relates to a method for the evaluation of binding affinity of biologically active substances for cardiolipin based on acridinium salt utilization as a fluorescent probe.

53. 发明申请

US20160216275A1 DIFFERENCE GEL ELECTROPHORESIS OF PHOSPHOPROTEOME 审中-公开
标题翻译：磷酸盐电泳的差异凝胶电泳
公开(公告)号：US20160216275A1
公开(公告)日：2016-07-28
申请号：US15004339
申请日：2016-01-22
申请人： PURDUE RESEARCH FOUNDATION
发明人： Weiguo Andy Tao , Linna Wang
IPC分类号： G01N33/68 , G01N27/447 , G01N21/64 , C07F7/28 , G01N33/58
CPC分类号： G01N33/6842 , C07D231/56 , C07F7/28 , G01N1/30 , G01N21/6428 , G01N21/6456 , G01N27/44726 , G01N27/44795 , G01N31/00 , G01N33/582 , G01N33/683 , G01N2021/6421 , G01N2021/6439 , G01N2021/7786 , G01N2440/14 , G01N2570/00
摘要： A novel imaging reagent and gel-based method for analyzing and comparing phosphoproteomes on the same gel are disclosed herein.
摘要翻译：本文公开了一种用于分析和比较同一凝胶上的磷酸化蛋白的新型显影剂和基于凝胶的方法。

54. 发明申请

US20150323537A1 ABSORBENT PAPER AND USE THEREOF FOR BREAST CANCER DETECTION 审中-公开
标题翻译：吸收纸和其用于乳腺癌检测的用途
公开(公告)号：US20150323537A1
公开(公告)日：2015-11-12
申请号：US14712846
申请日：2015-05-14
申请人： Atossa Genetics, Inc.
发明人： Steven C. Quay , Shu-Chih Chen
IPC分类号： G01N33/574 , G01N33/68
CPC分类号： G01N33/57415 , A61B10/0041 , A61B10/0045 , C12Q1/06 , G01N33/683 , G01N33/6836
摘要： Biological samples of mammary fluid or components thereof are obtained using a breast pump device coupled with an absorbent paper or membrane, optionally facilitated by administering oxytocin to the subject. The breast pump device stimulates expression of mammary fluid and provides for collection of diagnostic samples on the absorbent paper or membrane to evaluate breast disease, including cancer. The biological sample may include fluid containing one or more of cells or cellular components, proteins, glycoproteins, peptides, nucleotides or other desired constituents comprising a breast disease marker. Absorbent paper or membrane, and methods relating to the paper or membrane, and a breast pump device are also provided.
摘要翻译：乳液或其组分的生物样品使用与吸收纸或膜偶联的吸乳器装置获得，任选地通过向受试者施用催产素来促进。吸乳器装置刺激乳腺液的表达，并提供在吸收纸或膜上收集诊断样品以评估乳腺疾病，包括癌症。生物样品可以包含含有一种或多种细胞或细胞组分，蛋白质，糖蛋白，肽，核苷酸或包含乳腺疾病标记物的其它所需组分的流体。还提供了吸收纸或膜，以及与纸或膜有关的方法以及吸奶器装置。

55. 发明授权

US09052318B2 Absorbent paper and use thereof for breast cancer detection 有权
标题翻译：吸收纸及其用于乳腺癌检测的用途
公开(公告)号：US09052318B2
公开(公告)日：2015-06-09
申请号：US14352461
申请日：2012-10-24
申请人： Atossa Genetics, Inc.
发明人： Steven C. Quay , Shu-Chih Chen
IPC分类号： G01N31/00 , G01N33/53 , G01N33/574 , G01N33/68 , A61B10/00 , C12Q1/06
CPC分类号： G01N33/57415 , A61B10/0041 , A61B10/0045 , C12Q1/06 , G01N33/683 , G01N33/6836
摘要： Biological samples of mammary fluid or components thereof are obtained using a breast pump device coupled with an absorbent paper or membrane, optionally facilitated by administering oxytocin to the subject. The breast pump device stimulates expression of mammary fluid and provides for collection of diagnostic samples on the absorbent paper or membrane to evaluate breast disease, including cancer. The biological sample may include fluid containing one or more of cells or cellular components, proteins, glycoproteins, peptides, nucleotides or other desired constituents comprising a breast disease marker. Absorbent paper or membrane, and methods relating to the paper or membrane, and a breast pump device are also provided.
摘要翻译：乳液或其组分的生物样品使用与吸收纸或膜偶联的吸乳器装置获得，任选地通过向受试者施用催产素来促进。吸乳器装置刺激乳腺液的表达，并提供在吸收纸或膜上收集诊断样品以评估乳腺疾病，包括癌症。生物样品可以包含含有一种或多种细胞或细胞组分，蛋白质，糖蛋白，肽，核苷酸或包含乳腺疾病标记物的其它所需组分的流体。还提供了吸收纸或膜，以及与纸或膜有关的方法以及吸奶器装置。

56. 发明申请

US20140322744A1 CLONABLE TAG FOR CORRELATIVE LIGHT AND ELECTRON MICROSCOPY LABELING 审中-公开
标题翻译：相关灯和电子显微镜标签的可折叠标签
公开(公告)号：US20140322744A1
公开(公告)日：2014-10-30
申请号：US14359947
申请日：2012-11-27
申请人： INSTITUT CURIE , INSERM (INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE) , CENTRE NATIONAL DE LA RECHERCHE SCIENTIQUE
发明人： Nebraska Zambrano , Vincent Semetey , Sergio Marco
IPC分类号： C12Q1/02
CPC分类号： C12Q1/02 , C07K14/825 , C07K2319/00 , C07K2319/02 , G01N33/683
摘要： The present invention relates to a new clonable label for Correlative Light and Electron Microscopy based on the combination of modified metallothionein and lanthanides.
摘要翻译：本发明涉及基于改性金属硫蛋白和镧系元素组合的相关光和电子显微镜的新克隆标签。

57. 发明申请

US20130244264A1 Measurement Method Using Enzyme 有权
标题翻译：使用酶的测定方法
公开(公告)号：US20130244264A1
公开(公告)日：2013-09-19
申请号：US13792798
申请日：2013-03-11
申请人： ARKRAY, INC. , KIKKOMAN BIOCHEMIFA COMPANY
发明人： Koji Sugiyama , Satoshi Yonehara , Kazuhiko Shimoji
IPC分类号： C12Q1/37
CPC分类号： C12Q1/37 , C12Q1/54 , G01N33/526 , G01N33/683 , G01N33/723 , G01N33/92
摘要： A method for measuring an analyte is described that includes the steps of: i) preparing a reagent (D) in which an enzyme (A) and an enzyme (B) coexist in the absence of the analyte; ii) bringing the analyte into contact with the enzyme (A) and the enzyme (B) so that the enzyme (A) acts on the analyte to produce a product (E), on which the enzyme (B) does not substantially act, from the analyte; iii) producing a product (C) by allowing the enzyme (A) or an enzyme (F) that is different from the enzyme (A) that acts on the analyte to produce a product (C) to act on the analyte and/or the product (E); and iv) detecting the product (C) by the enzyme (B).
摘要翻译：描述了测量分析物的方法，其包括以下步骤：i）制备其中在不存在分析物的情况下酶（A）和酶（B）共存的试剂（D） ii）使分析物与酶（A）和酶（B）接触，使得酶（A）作用于分析物以产生酶（B）基本上不起作用的产物（E）来自分析物; iii）通过使酶（A）或不同于作用于分析物的酶（A）的酶（F））产生产物（C）以产生作用于分析物的产物（C）和/或产品（E）; 和iv）通过酶（B）检测产物（C）。

58. 发明申请

US20050142613A1 Test for the rapid evaluation of ischemic states and kits 审中-公开
标题翻译：测试缺血状态和试剂盒的快速评估
公开(公告)号：US20050142613A1
公开(公告)日：2005-06-30
申请号：US11033766
申请日：2005-01-12
申请人： David Bar-Or , Edward Lau , James Winkler , Gary Fagan , Hollie Wayment
发明人： David Bar-Or , Edward Lau , James Winkler , Gary Fagan , Hollie Wayment
IPC分类号： G01N33/68 , G01N33/53 , C07K14/765
CPC分类号： G01N33/6842 , G01N33/683
摘要： The present invention relates to rapid methods for the detection of ischemic states and to kits for use in such methods. Provided for is a rapid method of testing for and quantifying ischemia based upon methods of detecting and quantifying the existence of an alteration of the serum protein albumin which occurs following an ischemic event; methods for detecting and quantifying this alteration include evaluating and quantifying the cobalt binding capacity of circulating albumin, analysis and measurement of the ability of serum albumin to bind exogenous cobalt, detection and measurement of the presence of endogenous copper in a purified albumin sample and use of an immunological assay specific to the altered form of serum albumin which occurs following an ischemic event. Also taught by the present invention is the detection and measurement of an ischemic event by measuring albumin N-terminal derivatives that arise following an ischemic event, including truncated albumin species lacking one to four N-terminal amino acids or albumin with an acetylated N-terminal Asp residue.
摘要翻译：本发明涉及用于检测缺血状态的快速方法和用于这些方法的试剂盒。基于检测和定量缺血事件发生的血清蛋白白蛋白变化的存在的方法，提供了一种快速测试和定量缺血的方法; 用于检测和定量这种改变的方法包括评估和定量循环白蛋白的钴结合能力，分析和测量血清白蛋白结合外源钴的能力，检测和测量纯化白蛋白样品中内源性铜的存在，以及使用在缺血事件之后发生的血清白蛋白改变形式的特异性免疫测定。本发明还教导了通过测量缺血事件后出现的白蛋白N末端衍生物检测和测量缺血事件，包括缺乏一至四个N-末端氨基酸的截短的白蛋白物质或具有乙酰化N-末端的白蛋白 Asp残基。

59. 发明申请

US20050021235A1 Method and system for optically performing an assay to determine a medical condition 审中-公开
标题翻译：用于光学执行测定以确定医疗状况的方法和系统
公开(公告)号：US20050021235A1
公开(公告)日：2005-01-27
申请号：US10477384
申请日：2002-05-30
申请人： Raphael Bar-Or , David Bar-Or , C. Curtis
发明人： Raphael Bar-Or , David Bar-Or , C. Curtis
IPC分类号： A61B5/00 , G01N21/64 , G01N33/48 , G01N33/68 , G01N33/84
CPC分类号： G01N21/31 , A61B5/0071 , A61B5/0075 , A61B5/0084 , G01N21/6428 , G01N33/683 , G01N33/84 , G01N2021/6423 , G01N2201/129 , G01N2333/76 , G01N2800/2871 , G01N2800/32
摘要： A method and system are disclosed for detecting a medical condition wherein a blood or plasma sample is combined with a metal such as cobalt and optically analyzed for an optical distinction that identifies the medical condition. The invention is useful for diagnosing medical conditions such as ischemia. Moreover, the diagnoses of patient samples according to the invention may be enhanced by developing a mathematical model based on signal processing techniques such as principal component analysis on the data obtained in patient studies.
摘要翻译：公开了用于检测医疗状况的方法和系统，其中血液或血浆样品与诸如钴的金属组合并且被光学分析以识别医学状况的光学鉴别。本发明对于诊断诸如缺血的医学病症是有用的。此外，通过开发基于信号处理技术的数学模型，例如对患者研究中获得的数据的主成分分析，可以增强根据本发明的患者样品的诊断。

60. 发明申请

US20030215952A1 Tests for the rapid evaluation of ischemic states and kits 审中-公开
公开(公告)号：US20030215952A1
公开(公告)日：2003-11-20
申请号：US10414386
申请日：2003-04-15
申请人： Ischemia Technologies, Inc.
发明人： David Bar-Or , Edward Lau , James V. Winkler
IPC分类号： G01N033/20 , C07K014/675
CPC分类号： G01N33/6842 , G01N33/683 , Y10T436/107497
摘要： The present invention relates to rapid methods for the detection of ischemic states and to kits for use in such methods. Provided for is a rapid method of testing for and quantifying ischemia based upon methods of detecting and quantifying the existence of an alteration of the serum protein albumin which occurs following an ischemic event; methods for detecting and quantifying this alteration include evaluating and quantifying the cobalt binding capacity of circulating albumin, analysis and measurement of the ability of serum albumin to bind exogenous cobalt, detection and measurement of the presence of endogenous copper in a purified albumin sample and use of an immunological assay specific to the altered form of serum albumin which occurs following an ischemic event. Also taught by the present invention is the detection and measurement of an ischemic event by measuring albumin N-terminal derivatives that arise following an ischemic event, including truncated albumin species lacking one to four N-terminal amino acids or albumin with an acetylated N-terminal Asp residue.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式