专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

51. 发明申请

WO2006085972A3 COMPOSITIONS AND PROCESSES FOR THE EXTRACTION AND DETECTION OF MICROBIAL ATP 审中-公开
标题翻译：用于提取和检测微生物ATP的组合物和方法
公开(公告)号：WO2006085972A3
公开(公告)日：2007-01-04
申请号：PCT/US2005023545
申请日：2005-07-01
申请人： PROMEGA CORP , FAN FRANK , BUTLER BRAEDEN , WOOD KEITH V
发明人： FAN FRANK , BUTLER BRAEDEN , WOOD KEITH V
IPC分类号： C12Q1/00 , C12Q1/66 , G01N33/52 , G01N33/84
CPC分类号： C12Q1/66 , C12Q1/008 , C12Q1/02 , C12Q1/04 , G01N33/56916
摘要： The present invention is directed to compositions and methods for single step extraction and detection of ATP levels from microbial cells. The disclosed compositions are formulated to efficiently elicit bioluminescent detection of ATP among a broad variety of different microorganisms using a common single-step reagent composition. Additional luminescence-based methods are provided for identifying other useful extracting agents or for screening compounds for their pharmaceutical or biological effects on microbial cells.
摘要翻译：本发明涉及用于单步提取和检测来自微生物细胞的ATP水平的组合物和方法。使用常见的单步骤试剂组合物配制公开的组合物以有效引发各种不同微生物中的ATP的生物发光检测。提供额外的基于发光的方法用于鉴定其他有用的提取剂或筛选化合物对微生物细胞的药物或生物学作用。

52. 发明申请

WO2005033329A2 DETECTION OF LIVING CELLS IN POLYMERS OR PIGMENTS 审中-公开
标题翻译：检测聚合物或染料中的细胞
公开(公告)号：WO2005033329A2
公开(公告)日：2005-04-14
申请号：PCT/US2004031859
申请日：2004-09-23
申请人： EASTMAN CHEM CO
发明人： HOCHSTETLER SPENCER ERICH , MATOSKY ANDREW JOSEPH
IPC分类号： C12G1/04 , C12Q1/00 , C12Q1/04 , C12Q1/66
CPC分类号： C12Q1/66 , C12Q1/008 , C12Q1/04
摘要： Disclosed is a process for releasing ATP from living cells in aqueous mixtures of polymers or pigments. The aqueous mixture of a polymer or pigment is agitated in the presence of a particulate disruption agent to cause rupturing of the living cells and release of the ATP contained therein. Also disclosed is a process for detecting living cells in a aqueous mixtures of polymer or pigments by detecting the ATP released by the disruption process by a luciferin/luciferase assay. A kit for the detection of living cells in aqueous mixtures of polymers or pigments also is described.
摘要翻译：公开了一种从聚合物或颜料的水性混合物中的活细胞释放ATP的方法。聚合物或颜料的水性混合物在颗粒破坏剂的存在下搅拌以引起活细胞的破裂和其中所含的ATP的释放。还公开了通过荧光素/荧光素酶测定法检测由破坏过程释放的ATP来检测聚合物或颜料的水性混合物中的活细胞的方法。还描述了用于检测聚合物或颜料的水性混合物中的活细胞的试剂盒。

53. 发明申请

WO2004094656A1 ASSAY FOR DETECTING MYCOPLASMA BY MEASURING ACETATE KINASE OR CARBAMA KINASE ACTIVITY 审中-公开
标题翻译：通过测定乙酸激酶或卡巴肼激酶活性检测MYCOPLASMA的测定
公开(公告)号：WO2004094656A1
公开(公告)日：2004-11-04
申请号：PCT/GB2004/001647
申请日：2004-04-16
申请人： CAMBREX BIO SCIENCE NOTTINGHAM LIMITED , PITT, Anthony , CROUCH, Sharon, Patricia, Mary , SLATER, Kevin, John , COX, Anne
发明人： PITT, Anthony , CROUCH, Sharon, Patricia, Mary , SLATER, Kevin, John , COX, Anne
IPC分类号： C12Q1/04
CPC分类号： C12Q1/485 , C12Q1/008 , C12Q1/04 , G01N2333/30
摘要： The invention relates to a method of detecting the presence of mycoplasma in a test sample comprising: (i) providing a test sample; and (ii) detecting and/or measuring the activity of acetate kinase and/or carbamate kinase in the test sample, the activity being indicative of contamination by mycoplasma.
摘要翻译：本发明涉及一种检测测试样品中支原体存在的方法，包括：（i）提供测试样品; 和（ii）检测和/或测量测试样品中乙酸激酶和/或氨基甲酸酯激酶的活性，该活性指示由支原体污染。

54. 发明申请

WO99061655A1 TRANSITION STATE BINDING ASSAY 审中-公开
标题翻译：过渡状态绑定测试
公开(公告)号：WO99061655A1
公开(公告)日：1999-12-02
申请号：PCT/US1999/011916
申请日：1999-05-28
IPC分类号： C12Q1/00 , C12Q1/25 , C12Q1/37 , G01N33/573
CPC分类号： C12Q1/37 , C12Q1/008 , C12Q1/25 , G01N33/573 , G01N2333/96486 , G01N2500/20
摘要： A method to determine whether a test compound modulates the activity of an enzyme that has a metallated active site, comprising: a) providing said test compound coupled to a solid support; b) treating said solid support with the enzyme in metallated form; c) determining as a positive result of b) that the enzyme binds to said solid support and as a negative result of b) that said enzyme does not bind to said solid support; d) treating said solid support with said enzyme in a nonmetallated form; e) determining as a positive result of d) that the enzyme binds to said solid support and as a negative result of d) that said enzyme does not bind to said solid support; f) whereby a positive result in c) and a negative result in e) identifies said test compound as a modulator of said activity is disclosed.
摘要翻译：一种确定测试化合物是否调节具有金属化活性位点的酶的活性的方法，包括：a）提供与固体支持物偶联的所述测试化合物; b）用酶以金属化形式处理所述固体支持物; c）确定b）所述酶与所述固体支持物结合并作为b）所述酶不结合所述固体支持物的阴性结果的阳性结果; d）用所述酶以非金属化形式处理所述固体支持物; e）确定d）所述酶与所述固体支持物结合并作为d）否定结果的阳性结果，d）所述酶不结合所述固体支持物; f），其中c）中的阳性结果和e）中的阴性结果公开了所述测试化合物作为所述活性的调节剂。

55. 发明申请

WO1988004694A1 DIGITAL THRESHOLD COLOR CONTROL SYSTEM 审中-公开
标题翻译：数字门控颜色控制系统
公开(公告)号：WO1988004694A1
公开(公告)日：1988-06-30
申请号：PCT/US1987003335
申请日：1987-12-15
申请人： ENZYMATICS, INC.
发明人： ENZYMATICS, INC. , PALMER, John, L. , TIMMERMAN, Marsha
IPC分类号： C12Q01/00
CPC分类号： G01N33/521 , C12Q1/008 , Y10S436/904
摘要： A system for the digital quantitative colorimetric analysis of NAD(P)H and biological fluids and organic compounds that generate NAD(P)H when reacted with a specific dehydrogenase. An NAD(P)H-dependent chromogen reduction occurs, which results in a visible color change. A known quantity of a competing reactant for the NAD(P)H is used, which prevents the chromogen from reacting and changing color until the reactant is consumed, the quantity of which corresponds to the threshold concentration of the NAD(P)H or the compound reacting to generate NAD(P)H. Disposable devices and methods of use are also disclosed.
摘要翻译：用于与特定脱氢酶反应时产生NAD（P）H的NAD（P）H和生物流体和有机化合物的数字定量比色分析系统。 NAD（P）H依赖性色素减少发生，导致可见的颜色变化。使用已知量的用于NAD（P）H的竞争性反应物，其防止色原反应和变色，直到反应物消耗，其量相当于NAD（P）H的阈值浓度或化合物反应生成NAD（P）H。还公开了一次性装置和使用方法。

56. 发明授权

US09988663B2 Method for collecting biological material and device for collecting biological material 有权
公开(公告)号：US09988663B2
公开(公告)日：2018-06-05
申请号：US14434214
申请日：2013-10-18
申请人： HITACHI PLANT SERVICES CO., LTD.
发明人： Noe Miyashita , Shuichi Mori , Yuta Nakatsuka
IPC分类号： C12Q1/06 , C12M1/00 , C12M1/26 , C12M1/34 , C12Q1/00
CPC分类号： C12Q1/06 , C12M33/00 , C12M41/12 , C12M47/04 , C12N1/06 , C12Q1/008 , C12Q1/24 , C12Q1/6806 , G01N2035/00366
摘要： Provided is a device for collecting biological material from a microbe, the device including: a collection container that is used to collect a microbe from a liquid sample containing the microbe and to extract the biological material from the microbe; and a temperature control mechanism that controls a temperature of the collection container. Herein, the temperature control mechanism controls a temperature of the collection container so that the microbe is collected at a first temperature and the biological material is extracted from the microbe at a second temperature higher than the first temperature.

57. 发明申请

US20180106727A1 ULTRA-HIGH-SENSITIVE ASSAY OF PROTEIN AND NUCLEIC ACID AND KIT, AND NOVEL ENZYME SUBSTRATE 审中-公开
公开(公告)号：US20180106727A1
公开(公告)日：2018-04-19
申请号：US15845702
申请日：2017-12-18
申请人： Etsuro ITO , Satoshi WATABE , Toshiaki MIURA , Teruki YOSHIMURA
发明人： Etsuro ITO
IPC分类号： G01N21/78 , G01N21/25 , C12Q1/00 , C12Q1/34 , C12Q1/42 , C12Q1/28
CPC分类号： G01N21/78 , C12Q1/008 , C12Q1/28 , C12Q1/34 , C12Q1/42 , G01N21/25 , G01N2333/90209 , G01N2333/916 , G01N2333/924
摘要： Provided is a ultra-high-sensitivity assay in which the assay can be made on a commonly used assay apparatus such as an absorptiometer and a plate reader or with naked eyes. The high-sensitivity assay in which the assay can be made on a commonly used assay apparatus or with naked eyes can be provided by combining an enzyme cycling method using thio-NAD(P) as a coenzyme, a labeling enzyme and a substrate for the labeling enzyme optimally, and by amplifying thio-NAD(P)H, which is a signaling substance, exponentially and then quantifying the thio-NAD(P)H colorimetrically.

58. 发明授权

US09879306B2 Luciferase biosensors for cAMP 有权
公开(公告)号：US09879306B2
公开(公告)日：2018-01-30
申请号：US14695944
申请日：2015-04-24
申请人： PROMEGA CORPORATION
发明人： Brock Binkowski , Lance P. Encell , Monika G. Wood , Keith V. Wood , Kris Zimmerman , Paul Otto , Gediminas Vidugiris , Pete Stecha
IPC分类号： C12Q1/66 , C12Q1/00 , C12N9/02 , G01N33/573 , C12Q1/68 , G01N33/74
CPC分类号： C12Q1/66 , C07K2319/20 , C07K2319/61 , C12N9/0004 , C12N9/0069 , C12Q1/008 , C12Q1/6876 , C12Y113/12 , C12Y114/00 , G01N33/5735 , G01N33/74 , G01N2333/726 , G01N2333/90241
摘要： A modified luciferase protein which is a sensor for molecules including cAMP is provided. The modified luciferase protein includes one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with cAMP.

59. 发明申请

US20170321196A1 ANALYTICAL AND DIAGNOSTIC METHODS UTILIZING SHIGELLA FLEXNERI APYRASE 审中-公开
公开(公告)号：US20170321196A1
公开(公告)日：2017-11-09
申请号：US15524160
申请日：2015-11-06
申请人： ApiRays AB
发明人： Pavankumar Asalapuram , Aman Russom
IPC分类号： C12N9/14 , C12Q1/00 , C12Q1/34
CPC分类号： C12N9/14 , C12Q1/008 , C12Q1/34 , G01N2333/90 , G01N2333/914
摘要： A method, comprising the steps of providing a sample containing contaminating nucleoside diphosphates and/or nucleoside triphosphates, such as ATP and/or ATP analogues including deoxyribonucleoside triphosphates; reducing the amount of the contaminating nucleoside diphosphates and/or nucleoside triphosphates in the sample with an apyrase enzyme, wherein said apyrase enzyme is a Shigella flexneri apyrase; and performing an analysis of the sample, wherein said analysis comprises an assay that would have been affected by the contaminating nucleoside diphosphates and/or nucleoside triphosphates had they not been reduced in the reduction step.

60. 发明申请

US20160289728A1 METHOD FOR DETECTING FLUORESCENCE OR ABSORBANCE, METHOD FOR SUPPRESSING BACKGROUND, METHOD FOR MEASURING ADP, METHOD FOR MEASURING ACTIVITY OF ADP-SYNTHESIZING ENZYME, AND METHOD FOR MEASURING ACTIVITY OF GLUCOSYLTRANSFERASE 审中-公开
标题翻译：用于检测荧光或吸收的方法，用于抑制背景的方法，用于测量ADP的方法，用于测量ADP合成酶的活性的方法，以及用于测量葡糖基转移酶活性的方法
公开(公告)号：US20160289728A1
公开(公告)日：2016-10-06
申请号：US15037814
申请日：2014-11-21
申请人： The University of Tokyo
发明人： Kazuo KUMAGAI , Takayoshi OKABE , Hirotatsu KOJIMA , Tetsuo NAGANO
IPC分类号： C12Q1/00 , C12Q1/48 , C12Q1/42 , C12Q1/32
CPC分类号： C12Q1/008 , C12Q1/32 , C12Q1/42 , C12Q1/48 , C12Q1/485 , G01N2333/90209 , G01N2333/91091 , G01N2333/91097 , G01N2333/91215
摘要： In a method for detecting fluorescence or absorbance of the present invention, a diaphorase causes reduction from resazurin to resorufin in the presence of an SH reagent and NADH or NADPH, and the resulting fluorescence intensity or absorbance is measured. A method for measuring ADP of the present invention includes a 2-1 process in which glucose is reacted with ADP and an ADP-dependent hexokinase, a 2-2 process in which the glucose-6-phosphate obtained in the 2-1 process is reacted with NAD or NADP and glucose-6-phosphate dehydrogenase, and a 2-3 process in which resazurin is reacted with the NADH or NADPH obtained in the 2-2 process and a diaphorase in the presence of an SH reagent, and the resulting fluorescence intensity or absorbance is measured.
摘要翻译：在本发明的检测荧光或吸光度的方法中，在SH试剂和NADH或NADPH的存在下，心肌黄酶引起从青紫红素降低至芦荟碱，并且测量所得的荧光强度或吸光度。本发明的ADP测定方法包括使葡萄糖与ADP和ADP依赖性己糖激酶反应的2-1工序，其中2-1工序中得到的葡萄糖-6-磷酸为与NAD或NADP和葡萄糖-6-磷酸脱氢酶反应，和2-3次方法，其中天竺葵素与在2-2方法中获得的NADH或NADPH反应，在SH试剂存在下与心肌黄酶反应，测量荧光强度或吸光度。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式