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    • 57. 发明申请
    • Mutated Alkaline Cellulase
    • 突变的碱性纤维素酶
    • US20130029897A1
    • 2013-01-31
    • US13583508
    • 2011-03-11
    • Ayako ShimazuKeiji EndoMitsuyoshi Okuda
    • Ayako ShimazuKeiji EndoMitsuyoshi Okuda
    • C12N9/42C12N1/21C11D3/386C12N1/19C12N5/10C12N15/63C12N15/56
    • C11D3/38645C12N9/2437C12Y302/01004
    • The present invention provides a mutant alkaline cellulase having enhanced anti-redeposition ability. The present invention provides a mutant alkaline cellulase having an amino acid sequence in which at least amino acid residue selected from the amino acid residues at the positions corresponding to positions 58, 45, 52, 56, 60, 64, 66, 71, 103, 119, 122, 123, 124, 125, 127, 130, 140, 161, 164, 175, 176, 178, 179, 181, 193, 194, 195, 196, 197, 199, 202, 203, 217, 225, 227, 228, 251, 267, 272, 276, 277, 280, 282, 297, 310, 312, 318, 324, 345, 354, 356, 357, 360, 363, 368, 418, 419, 420, 421, 422, 453, 454, 455, 457, 458, 459, 494, 495, 496, 500, 501, 502, 503, 504, 550, 551, 552, 604, 605, 606, 607, 608, 640, 641, 642, 644, 645, 646, 683, 684, 685, 690, 691, 692, 693, 694, 739, 740, and 741 of the amino acid sequence of an alkaline cellulase set forth in SEQ ID NO: 2 or of the amino acid sequence of an alkaline cellulase having at least 70% identity with the amino acid sequence of SEQ ID NO: 2, is substituted with other amino acid residue.
    • 本发明提供具有增强的抗再沉积能力的突变型碱性纤维素酶。 本发明提供了一种具有氨基酸序列的突变型碱性纤维素酶,其中至少选自氨基酸残基的氨基酸残基位于对应于位置58,45,52,56,66,66,61,103,60,64,66,67,60,62,68,62,62,64,68,62,62,62,67,67,69,68,62,62,67 119,122,123,124,125,127,130,140,​​161,164,175,176,178,179,181,193,194,195,196,197,199,202,203,217,225, 227,228,251,267,272,276,277,280,282,277,310,312,318,324,345,354,356,357,360,363,368,418,419,420,421,418,417,318,472,427,268,267,280,282,276,277,280,282,276,277,280,282,276,277,280,282,276,277,280,282,276,277,280,282 422,453,454,455,457,458,459,494,495,496,5500,501,502,503,504,550,551,552,604,605,606,607,608,640,641, 或SEQ ID NO:2所示的碱性纤维素酶的氨基酸序列的642,644,645,646,683,684,685,690,691,692,693,694,739,740和741,或 与SEQ ID NO:2的氨基酸序列具有至少70%同一性的碱性纤维素酶的氨基酸序列被其它氨基酸残基取代。
    • 58. 发明申请
    • Alkaline Protease Variants
    • 碱性蛋白酶变体
    • US20120058928A1
    • 2012-03-08
    • US13266525
    • 2010-04-26
    • Masatoshi TohataMitsuyoshi OkudaTsuyoshi SatoKeiji EndoKatsuhisa Saeki
    • Masatoshi TohataMitsuyoshi OkudaTsuyoshi SatoKeiji EndoKatsuhisa Saeki
    • C12N9/50C12N15/63C12P21/02C12N1/19C12N1/15C11D3/386C12N15/57C12N1/21
    • C11D3/38618C11D3/386C11D3/38681C12N9/54
    • An alkaline protease variant derived from an alkaline protease consisting of an amino acid sequence represented by SEQ ID NO: 2 or consisting of an amino acid sequence having an identity of 90% or more therewith, which variant has mutations wherein one or more amino acid residues at positions selected from (a) position 6, (b) position 15, (c) position 16, (d) position 65, (e) position 66, (f) position 82, (g) position 83, (h) position 204, (i) position 319, and (j) position 337 of the amino acid sequence represented by SEQ ID NO: 2, or at positions corresponding thereto are substituted with the following amino acid residues: (a) or a position corresponding thereto: Typ, Leu, Val, Ile, Met, Tyr, Gln, Lys, Thr, Phe, Arg, Ser, Cys, Ala, or His; (b) or a position corresponding thereto: Glu, Met, Asp, Val, Gln, Arg, Cys, Trp, Ala, or Phe; (c) or a position corresponding thereto: Met, Glu, Arg, Val, Lys, Phe, Tyr, Ile, His, Asp, or Cys; (d) or a position corresponding thereto: Trp; (e) or a position corresponding thereto: His, Trp, Ser, or Leu; (f) or a position corresponding thereto: Ala, Glu, Gln, Ser, Cys, Gly, H is, Lys, Arg, Met, or Asn; (g) or a position corresponding thereto: Ala, Ser, or Cys; (h) or a position corresponding thereto: Glu, Asp, Cys, Val, Thr, Pro, His, Ile, Trp, Ser, Asn, Lys, or Arg; (i) or a position corresponding thereto: Trp, Val, Thr, Leu, Ile, Cys, Glu, Lys, Tyr, Arg, Phe, Gln, Met, Pro, Asp, Asn, H is, or Ser; and (j) or a position corresponding thereto: Arg, Gly, Ser, Lys, Gln, Thr, His, Ala, Cys, or Val.
    • 由碱性蛋白酶衍生的碱性蛋白酶变体,其由SEQ ID NO:2所示的氨基酸序列组成或由与其同一性为90%以上的氨基酸序列组成,所述变体具有突变,其中一个或多个氨基酸残基 在从(a)位置6,(b)位置15,(c)位置16,(d)位置65,(e)位置66,(f)位置82,(g)位置83,(h) 204,(i)位置319和(j)由SEQ ID NO:2表示的氨基酸序列的位置337或与其相对应的位置被以下氨基酸残基取代:(a)或与其对应的位置: Typ,Leu,Val,Ile,Met,Tyr,Gln,Lys,Thr,Phe,Arg,Ser,Cys,Ala或His; (b)或相应位置:Glu,Met,Asp,Val,Gln,Arg,Cys,Trp,Ala或Phe; (c)或对应的位置:Met,Glu,Arg,Val,Lys,Phe,Tyr,Ile,His,Asp或Cys; (d)或与之对应的位置:Trp; (e)或与之对应的位置:His,Trp,Ser或Leu; (f)或对应位置:Ala,Glu,Gln,Ser,Cys,Gly,H is,Lys,Arg,Met或Asn; (g)或对应的位置:Ala,Ser或Cys; (h)或相应位置:Glu,Asp,Cys,Val,Thr,Pro,His,Ile,Trp,Ser,Asn,Lys或Arg; (i)或与其相对应的位置:Trp,Val,Thr,Leu,Ile,Cys,Glu,Lys,Tyr,Arg,Phe,Gln,Met,Pro,Asp,Asn,H is或Ser; 和(j)或对应的位置:Arg,Gly,Ser,Lys,Gln,Thr,His,Ala,Cys或Val。
    • 60. 发明申请
    • Herbicide Composition for Paddy Field
    • 稻田除草剂组成
    • US20110059847A1
    • 2011-03-10
    • US12943312
    • 2010-11-10
    • Keiji EndoShinichi ShirakuraShin NakamuraNatsuko Minegishi
    • Keiji EndoShinichi ShirakuraShin NakamuraNatsuko Minegishi
    • A01N25/32A01N43/88A01P13/00
    • A01N43/54A01N43/66A01N25/32A01N37/18A01N37/22A01N39/02A01N43/08A01N43/12A01N43/56A01N43/78A01N43/80A01N43/86A01N43/90A01N47/30A01N47/36A01N47/38A01N57/14A01N2300/00
    • A herbicide composition for paddy field characterized by containing (a) a herbicidal difluoromethanesulfonamide derivative represented by the formula (I) and (b) at least one herbicidal compound selected from the group consisting of pretilachlor, butachlor, alachlor, metolachlor, acetochlor, clomeprop, bromobutide, benfuresate, indanofan, pyrazolate, benzofenap, pyrazoxyfen, pyraclonil, oxaziclomefone, bensulfuron-methyl, azimsulfuron, imazosulfuron, pyrazosulfuron-ethyl, cyclosulfamuron, ethoxysulfuron, halosulfuron-methyl, orthosulfamuron, cinosulfuron, metsulfuron-methyl, penoxsulam, thiobencarb, pyributycarb, molinate, dimethametryn, simetryn, cafenstrole, quinclorac, anilofos, mefenacet, fentrazamide, pentoxazone, oxadiargyl, oxadiazon, benzobicyclon, mesotrione, AVH301 (code no.), cyhalofop-butyl, metamifop, bispyribac-sodium, pyriftalid, pyrimisulfan, pyrimenobac-methyl, chlormethoxynil, oxyfluorfen, dithiopyr, MCPA, MCPB, 2,4-D, dymron, cumyluron, quinoclamine and clomazone, and/or (c) one or more compounds selected from the group consisting of safeners dymron, isoxadifen(-ethyl), flurazole, fenchlorazole-ethyl, fenclorim, cloquintocet-mexyl, oxabetrinil, fluxofenim, mefenpyr-diethyl, furilazole, R-29148 (code no.), benoxacor, dichlormid and dicyclonone as effective components.
    • 一种用于稻田的除草剂组合物,其特征在于含有(a)由式(I)和(b)表示的除草二氟甲磺酰胺衍生物,至少一种选自草酰草胺,丁草胺,甲草胺,异丙甲草胺,乙草胺,氯米普, 溴替丁,苯呋酮,吡唑啉,吡嘧磺隆,苯唑芬酮,吡嘧磺隆,吡嘧磺隆,恶唑磺隆,吡嘧磺隆,吡嘧磺隆,咪唑磺隆,吡嘧磺隆,环磺胺嘧啶,乙氧基磺隆,卤代磺隆,邻氨基磺隆, 茉莉酸,二甲基苯甲酸,西妥昔单抗,喹诺酮,芬太拉唑,依诺沙星,恶草精,恶二酮,苯并环己酮,甲基磺草酮,AVH301(编号),cyhalofop-butyl,metamifop,bispyribac-sodium,pyriftalid, ,氯氟乙氧基,氧氟芬,二硫代吡啶,MCPA,MCPB,2,4-D,胞嘧啶,异戊肾上腺素,喹诺酮和克罗莫他, d /或(c)一种或多种选自安息剂dymron,异恶草酮( - 乙基),氟尿嘧啶,芬氯唑,芬舍洛芬,氯喹毒素,奥沙利坦,氟吡芬,咪芬吡啶二乙基,呋喃唑,R-29148 (代号),benoxacor,dichlormid和dicyclonone作为有效成分。