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51. 发明专利

JPH0455671B2 失效
公开(公告)号：JPH0455671B2
公开(公告)日：1992-09-04
申请号：JP19160384
申请日：1984-09-14
申请人： HITACHI LTD
发明人： SHIMIZU NORIO , OTAHARA YOJI , FUJIWARA KYOSHI
IPC分类号： C12N15/09 , C12M1/36 , C12N1/20 , C12N1/21 , C12N9/00 , C12N15/00 , C12P21/00 , C12R1/19

52. 发明专利

JPH02242669A METHOD FOR CONTROLLING CULTURE ENVIRONMENTAL CONDITION FOR ANIMAL CELL, CONTROLLER, METHOD FOR CULTURING AND CULTURE DEVICE 失效
公开(公告)号：JPH02242669A
公开(公告)日：1990-09-27
申请号：JP5972189
申请日：1989-03-14
申请人： HITACHI LTD
发明人： TAKEUCHI KOJI , KAWAGUCHI HIDEO , ISHIBASHI HITOSHI , SHIMIZU NORIO
IPC分类号： C12M3/00 , C12N3/00 , C12N5/07 , C12N5/071 , C12Q3/00
摘要： PURPOSE:To enable obtaining of a method and apparatus for stably culturing animal cells for a long period by calculating the ratio of the amount of formed lactic acid to the amount of consumed glucose in culturing the animal cells and controlling culture environmental conditions. CONSTITUTION:The concentrations of glucose and lactic acid in a culture solution of animal cells are measured to provide measured values, which are then used to afford the ratio (L/G) of the amount of the formed glucose to the amount of the consumed glucose. If the ratio (L/G) deviates from a prescribed range of 0-0.6 (not including 0), the dissolved oxygen concentration or glucose concentration or both are controlled by increasing the dissolved oxygen concentration and/or decreasing the glucose concentration in the culture solution. A culture device having a controller for the above-mentioned culture environmental conditions is used to carry out culture of animal cells by the aforementioned controlling method. Thereby, the culture environmental conditions of the animal cells can be stably controlled for a long period. As result, the animal cells can be stably cultured for the long period.

53. 发明专利

JPH029793B2 失效
公开(公告)号：JPH029793B2
公开(公告)日：1990-03-05
申请号：JP22397184
申请日：1984-10-26
申请人： HITACHI LTD
发明人： KATO KENJI , TOO KUNIHIKO , SUMYA TOMOAKI , SHIMIZU NORIO
IPC分类号： C12N1/00 , C12N1/20 , C12R1/19

54. 发明专利

JPS615709B2 JPS615709B2 - 失效
公开(公告)号：JPS615709B2
公开(公告)日：1986-02-20
申请号：JP6743082
申请日：1982-04-23
申请人： Hitachi Ltd
发明人： SHIMIZU NORIO , UENO MASAO , OTAHARA YOJI , SUMYA TOMOAKI , FUJIMOTO MASAKATSU , FUJIWARA KYOSHI
IPC分类号： C12N1/00 , C12M1/36 , C12N1/16
CPC分类号： C12M41/32 , C12M41/34

55. 发明专利

JPS619282A Method for cultivating genetic recombinant microorganism 失效
标题翻译：用于培养遗传重组微生物的方法
公开(公告)号：JPS619282A
公开(公告)日：1986-01-16
申请号：JP12747284
申请日：1984-06-22
申请人： Hitachi Ltd
发明人： SHIMIZU NORIO , MASUDA KEIKO , KURIHARA TOSHIHARU , OTAHARA YOUJI
IPC分类号： C12N15/09 , C12N1/20 , C12N1/21 , C12N9/38 , C12N15/00 , C12R1/19
摘要： PURPOSE: To obtain efficiently a product of the titled microorganism in a large amount, by cultivating a microorganism having a complex plasmid consisting of the aimed gene, vector and promoter and the expression ability thereof in a culture medium containing an inhibitor substance and an inducer substance to control the promoter activity.
CONSTITUTION: A microorganism having the aimed gene, vector and promoter in the cell and an expression ability of the aimed gene is cultivated in a culture medium containing an inhibitor substance and inducer substance to control the promoter activity and give the product of the aimed gene efficiently in a large amount. For example, a DNA fragment containing a trp promoter cut out of PTREI is linked to an β-gal gene with an enzyme to create a complex plasmid PTREZI, and tryptophan (trp) is added thereto in the initial period of cultivation to suppress the activity of the trp promoter, reduce the production of the β-gal and multiply the microbial cells. An inducer substance for starting the activity of the trp promoter is then added to produce the β-gal.
COPYRIGHT: (C)1986,JPO&Japio
摘要翻译：目的：通过培养具有目标基因，载体和启动子组成的复合质粒的微生物及其在含有抑制物质和诱导物质的培养基中的表达能力，有效地获得大量标题微生物的产物以控制启动子活性。构成：在含有抑制剂物质和诱导物质的培养基中培养具有目的基因，细胞中的载体和启动子和靶基因的表达能力的微生物，以控制启动子活性并有效地产生目的基因的产物大量的。例如，含有从PTREI切出的trp启动子的DNA片段与酶的β-gal基因连接以产生复合质粒PTREZI，并且在培养的初始阶段向其中加入色氨酸（trp）以抑制活性的trp启动子，减少β-gal的产生并繁殖微生物细胞。然后加入用于启动trp启动子活性的诱导物质以产生β-gal。

56. 发明专利

JPS5898085A CULTIVATION METHOD OF MICROORGANISM IN HIGH YIELD 失效
公开(公告)号：JPS5898085A
公开(公告)日：1983-06-10
申请号：JP19451781
申请日：1981-12-04
申请人： HITACHI LTD
发明人： MATSUSHITA NOBUO , SAIKAI MASAHARU , FUJIMOTO MASAKATSU , SHIMIZU NORIO , UENO MASAO
IPC分类号： C12M1/36 , C12N1/00
摘要： PURPOSE:To cultivate a microorganism in high yield, by measuring the amount of an added pH adjustor, and controlling the feed rate of a substrate on the basis of the amount of the added pH adjustor. CONSTITUTION:The pH a culture medium 25 in the cultivation is controlled to be always at the set value by a pH adjusting apparatus 13, and the rate of change in pH is proportional to the amount of a pH adjustor required for controlling the pH of the culture medium 25 at the set value by adding the pH adjustor from the pH adjusting apparatus 13 to be culture medium 25. Thus, the amount of the pH adjustor added by one opening of a solenoid valve in the pH adjusting apparatus 13 is kept constant, and the amount of the added pH adjustor is determined by multiplying the frequency of addition obtained by integrating the frequencies of the addition of the pH adjustor for a given period by the constant amount of the pH adjustor added to the culture medium 25 on one opening of the solenoid valve in the pH adjusting apparatus 13. The measured amount of the added pH adjustor is calculated by a minicomputer 24 from the operational expression on the basis of the amount of the added pH adjustor to output controlling output signals from the minicomputer 24 to a flow rate variable pump 14 to feed the substrate to the culture medium 25 at a proper rate.

57. 发明专利

JPS57194260A PREVENTING METHOD FOR CORROSION 失效
公开(公告)号：JPS57194260A
公开(公告)日：1982-11-29
申请号：JP7931281
申请日：1981-05-27
申请人： HITACHI LTD
发明人： IZUMITANI MASAKIYO , KAWAKAMI TOSHIO , MINATO AKIRA , SHIMIZU NORIO , OOSUMI KATSUMI
IPC分类号： C23F11/18
摘要： PURPOSE:To inhibit the activity of an organism (nitrobacteria) converting nitrite ions into nitrate ions by regulating the concn. of nitrite to be added to water as a corrosion inhibitor for carbon steel of a machine or an apparatus using water to a prescribed value or above. CONSTITUTION:In a machine or an apparatus made of carbon steel and using water such as a recirculating water type cooler, the concn. of nitrite ions in circulating water contacting with the machine or the apparatus is kept at >=500ppm. Nutrition for nitrobacteria is made excess to cause a substrate trouble, thereby inhibiting the activity of nitrobacteria. Thus, the concn. of nitrite ions is not reduced, and the corrosion inhibiting action is maintained.

58. 发明专利

JPS5736982A Cultivation of baker's yeast 失效
标题翻译：空值
公开(公告)号：JPS5736982A
公开(公告)日：1982-02-27
申请号：JP11166880
申请日：1980-08-15
申请人： Hitachi Ltd
发明人： UENO MASAO , SHIMIZU NORIO , YAMAGUCHI TETSUO , SAITO SETSUO , OTAHARA YOJI
IPC分类号： C12N1/00 , C12N1/16 , C12N1/18
摘要： PURPOSE: To control the rate of feeding of substrate in the aerated cultivation of baker's yeast so as to conduct the feed of the cultivation substrate at the optimum rate, by detecting the production of ethanol from the data obtained by the measurement of the feed gas flow and the exhaust gas flow.
CONSTITUTION: The feed gas flow rate and the exhaust gas flow rate are measured continuously during the cultivation by the feed gas flow meter 5 and the exhaust gas flow meter 6, respectively. The measured data are inputted to a comparing means 7, in which the ratio of the flow rates is calculated, and the comparative relationship of the rates is displayed by the displaying means 8. When the exhaust gas flow rate is larger than the feed gas flow rate, it is supposed that ethanol is produced by the excessive feed of the substrate, and the substrate feed rate is reduced by operating the substrate feed rate controlling means 3. On the contrary, when the exhaust gas flow rate is smaller, the substrate feed rate is incresed. The control can be performed automatically, by electrically connecting the comparing means with the substrate feed rate controlling means.
COPYRIGHT: (C)1982,JPO&Japio
摘要翻译：目的：为了控制面包酵母充气培养中的底物喂养速度，以最佳速率进行培养基质的进料，通过从进料气流测量获得的数据中检测出乙醇的产生和废气流。构成：在进料气体流量计5和废气流量计6的培养期间，分别测量进料气体流量和废气流量。测量数据被输入到比较装置7，在比较装置7中，计算出流量的比率，并通过显示装置8显示速率的比较关系。当废气流量大于进料气体流量速率，假设通过基板的过量进料产生乙醇，并且通过操作基板进给速率控制装置3来降低基板进给速率。相反，当排气流量较小时，基板进给价格上涨。可以通过将比较装置与基板进给速率控制装置电连接来自动执行控制。

59. 发明专利

JPS56144086A CULTIVATION OF MICROORGANISM IN HIGH CONCENTRATION OF MOLD 失效
公开(公告)号：JPS56144086A
公开(公告)日：1981-11-10
申请号：JP4563680
申请日：1980-04-09
申请人： HITACHI LTD
发明人： SHIMIZU NORIO , YAMAGUCHI TETSUO , SAITOU SETSUO , UENO MASAO , OTAHARA YOUJI
IPC分类号： C12N1/00 , C12R1/78 , C12R1/865
摘要： PURPOSE:To cultivate molds in high concentration without causing contamination resulting from various germs and to intend rise in yield of mold, by returning partly molds as seed molds prepared from processes after washing process after cultivation to a culture tank. CONSTITUTION:A medium and oxygen are fed to the culture bath 1 and the cultivation of microorganisms is carried out aerobically with stirring. Molds are sent to the centrifugal separator 6, washed with cleaning water and separated, concentrated by the filter press 11, and dried to give a product. The molds prepared from the processes after washing process as seed microorganisms are partly returned to the cultivation tank 1 by the return pipe 10. Thus cultivation with an initial concentration >=20g-dry cell/l is carried out. This method is used for cultivation of various kinds of yeast, bacilli, molds, and ray fungi.

60. 发明专利

JPS55129195A FILAMENTOUS BACTERIA EXTERMINATING METHOD IN ACTIVATED SLUDGE TREATMENT 失效
公开(公告)号：JPS55129195A
公开(公告)日：1980-10-06
申请号：JP3556079
申请日：1979-03-28
申请人： HITACHI LTD
发明人： SAITOU SETSUO , SHIMIZU NORIO , OTAHARA YOUJI
IPC分类号： C02F3/12
摘要： PURPOSE:To recover the settleability of activated sludge by raising the osmotic press. of liquor in an aeration tank to 5 atm or more to exterminate filamentous bacteria propagated in the sludge. CONSTITUTION:By adding salts such as table salt or sodium sulfate to water treated with activated carbon whose settleability has been deteriorated by propagation of filamentous bacteria, the osmotic press. of the water is raised to 5 atm or more to exterminate the bacteria. When the amt. of the salts added is known, the osmotic press. is obtd. almost approximately (dissolvable org. constituents are neglected) according to the following van't Hoff's equation of osmotic press.: pi=i.R.T.C (where pi is osmotic press., i is the van't Hoff's factor, R is the gas constant, T is the absolute temp. and C is the solute mol no.). When the concn., etc. of the salts in the water are not clear, the amt. of the salts to be added can be adjusted with an osmometer so that the water becomes the predetermined osmotic press.

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