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51. 发明申请

US20030157519A1 Recombinant renilla reniformis system for bioluminescence resonance energy transfer 审中-公开
标题翻译：用于生物发光共振能量转移的重组肾素肾脏系统
公开(公告)号：US20030157519A1
公开(公告)日：2003-08-21
申请号：US10265031
申请日：2002-10-04
申请人： Stratagene
发明人： Vivan Q. Zhang , Peter E. Vaillancourt
IPC分类号： C12Q001/68 , G01N033/53 , G01N033/537 , G01N033/543 , C12Q001/66 , C12N009/06 , C07H021/04 , C12P021/02 , C12N005/06
CPC分类号： C12N9/0069 , C07K14/43595 , C07K2319/00 , G01N33/542 , G01N2500/02
摘要： The invention relates to compositions comprising a first fusion protein comprising a first polypeptide domain and a R. reniformis luciferase and a second fusion protein comprising a second polypeptide domain and a R. reniformis GFP. The invention also relates to compositions comprising one or more polynucleotides encoding a first fusion protein comprising a first polypeptide domain and a R. reniformis luciferase and a second fusion protein comprising a second polypeptide domain and a R. reniformis GFP. The invention also relates to methods and kits for detecting protein-protein interactions, determining the location of a protein-protein interaction, identifying cells wherein there is a protein-protein interaction of interest, and screening for a candidate modulator that increases or decreases the amount of a protein-protein interaction.
摘要翻译：本发明涉及包含第一融合蛋白质的组合物，所述第一融合蛋白质包含第一多肽结构域和罗氏扁桃体荧光素酶，以及第二融合蛋白，其包含第二多肽结构域和红纹氏杆菌GFP。本发明还涉及包含一种或多种编码包含第一多肽结构域和雷氏镜状荧光素酶的第一融合蛋白的多核苷酸的组合物，以及包含第二多肽结构域和红纹氏杆菌GFP的第二融合蛋白。本发明还涉及用于检测蛋白质 - 蛋白质相互作用的方法和试剂盒，确定蛋白质 - 蛋白质相互作用的位置，鉴定存在感兴趣的蛋白质 - 蛋白质相互作用的细胞，以及筛选增加或减少量的候选调节剂的蛋白质 - 蛋白质相互作用。

52. 发明申请

US20030149257A1 Compositions and methods for polynucleotide sequence determination 失效
标题翻译：用于多核苷酸序列测定的组合物和方法
公开(公告)号：US20030149257A1
公开(公告)日：2003-08-07
申请号：US10056598
申请日：2002-01-24
申请人： Stratagene
发明人： Joseph A. Sorge , Bahram Arezi , Holly Hogrefe
IPC分类号： C12Q001/68 , C07H021/04
CPC分类号： C12Q1/6827 , C12Q1/6818 , C12Q1/6858 , C12Q2535/125 , C12Q2525/161 , C12Q2565/101
摘要： The present invention relates to a method for identifying a nucleotide at a predetermined location on a target polynucleotide. The method involves single nucleotide extension reaction comprising an oligonucleotide primer comprising a first sequence and a second sequence or a tag. The method may further comprises a probe which hybridizes to the second sequence or an anti-tag molecule which interacts with the tag, where the hybridization or interaction causes a detectable signal transfer which is indicative of the identity of the nucleotide base at the predetermined location. The invention further provides compositions and kits for performing the subject method of the invention.
摘要翻译：本发明涉及鉴定靶多核苷酸上预定位置的核苷酸的方法。该方法涉及包含含有第一序列和第二序列或标签的寡核苷酸引物的单核苷酸延伸反应。该方法还可以包括与第二序列杂交的探针或与标签相互作用的抗标签分子，其中杂交或相互作用引起可检测的信号转移，其指示在预定位置处的核苷酸碱基的身份。本发明还提供了用于实施本发明的主题方法的组合物和试剂盒。

53. 发明申请

US20020197642A1 Primer-mediated polynucleotide synthesis and manipulation techniques 审中-公开
标题翻译：引物介导的多核苷酸合成和操作技术
公开(公告)号：US20020197642A1
公开(公告)日：2002-12-26
申请号：US10211341
申请日：2002-08-05
申请人： Stratagene
发明人： Joseph A. Sorge , Kerstien A. Padgett
IPC分类号： C12Q001/68 , C12P019/34
CPC分类号： C12N15/66 , C12N15/10 , C12Q1/686 , C12Q2525/131 , C12Q2525/117
摘要： The invention provides improved techniques for conveniently manipulating polynucleotides of interest without the need to rely upon the presence of naturally occurring restriction sites. Additionally, using the methods and primers of the invention, one may synthesize a polynucleotide of interest in a form which is easily and directionally cloned into a DNA sequence of choice without necessarily introducing extraneous nucleotides in the final polynucleotide product. The methods of the invention employ releasable primers that comprise a recognition site for a releasing enzyme joined to a region for annealing to the polynucleotide template of interest. Polynucleotide sequences of interest are synthesized using one or more synthesis primers, wherein at least one of the primers is a releasable primer. After synthesis, the synthesis product is cleaved by a releasing enzyme. In a preferred embodiment of the invention, inhibitory base analogs are incorporated in the synthesis product to protect against the formation of unwanted internal cleavage products. In another embodiment of the invention, at least one of the releasable primers is bound to an immobilizing solid phase support so as to produce immobilized synthesis products that may be conveniently released by a releasing enzyme. Another aspect of the invention is to provide releasable primers and kits for performing the subject methods. Typically, such kits may comprise a releasing enzyme and one or more reagents for performing a polynucleotide synthesis reaction, preferably a cyclic amplification reaction.
摘要翻译：本发明提供了用于方便地操作感兴趣的多核苷酸的改进的技术，而不需要依赖天然存在的限制性位点的存在。另外，使用本发明的方法和引物，人们可以以容易且定向克隆到选择的DNA序列中的形式合成感兴趣的多核苷酸，而不必在最终多核苷酸产物中引入外来核苷酸。本发明的方法使用可释放的引物，其包含连接到用于退火的感兴趣的多核苷酸模板的区域的释放酶的识别位点。使用一种或多种合成引物合成感兴趣的多核苷酸序列，其中至少一种引物是可释放引物。合成后，合成产物被释放酶切割。在本发明的优选实施方案中，将抑制性碱基类似物掺入合成产物中以防止形成不需要的内部裂解产物。在本发明的另一个实施方案中，至少一个可释放的引物与固定的固相载体结合，以产生可通过释放酶方便地释放的固定的合成产物。本发明的另一方面是提供用于实施本发明方法的可释放的底漆和试剂盒。通常，这样的试剂盒可以包含释放酶和用于进行多核苷酸合成反应的一种或多种试剂，优选循环扩增反应。

54. 发明申请

US20040188411A1 Apparatus and method for flexible heating cover assembly for thermal cycling of samples of biological material 有权
标题翻译：用于生物材料样品热循环的柔性加热盖组件的装置和方法
公开(公告)号：US20040188411A1
公开(公告)日：2004-09-30
申请号：US10811663
申请日：2004-03-29
申请人： Stratagene
发明人： Larry R. Brown , William D. Brumley , Kenneth J. Zajac
IPC分类号： F27D011/00 , H05B003/06
CPC分类号： B01L3/50851 , B01L3/50853 , B01L7/52 , B01L7/54 , B01L2300/046 , B01L2300/0829 , B01L2300/1822 , B01L2300/1827 , B01L2300/1844
摘要： A flexible heating cover assembly for an apparatus for heating samples of biological material with substantial temperature uniformity includes a housing having a plurality of engageable enclosure components; a resistive heater having a plurality of heater element areas; a heater backing plate providing stability to the resistive heater; a force distribution system that distributes a force over the heater backing plate; and a support plate providing stiffness for the force distribution system, wherein the arrangement of the resistive heater, the heater backing plate, the force distribution system and the support plate provide substantial temperature uniformity among a plurality of sample tubes for receiving samples of biological material. The flexible heating cover assembly improves the uniformity, efficiency, quality, reliability and controllability of the thermal response during thermal cycling of the biological material.
摘要翻译：一种用于加热具有显着温度均匀性的生物材料样品的装置的柔性加热盖组件包括具有多个可接合外壳部件的壳体; 电阻加热器，具有多个加热元件区域; 加热器背板，为电阻加热器提供稳定性; 力分配系统，其在加热器背板上分布力; 以及为力分配系统提供刚度的支撑板，其中电阻加热器，加热器背板，力分配系统和支撑板的布置在用于接收生物材料样品的多个样品管之间提供显着的温度均匀性。柔性加热盖组件改善了生物材料热循环期间的热响应的均匀性，效率，质量，可靠性和可控性。

55. 发明申请

US20040137628A1 Selection for more efficient transformation host cells 审中-公开
标题翻译：选择更有效的转化宿主细胞
公开(公告)号：US20040137628A1
公开(公告)日：2004-07-15
申请号：US10686181
申请日：2003-10-14
申请人： Stratagene
发明人： Alan L. Greener
IPC分类号： C12N015/87 , C12N015/74
CPC分类号： C12R1/19 , C12N13/00 , C12N15/01 , C12N15/64 , C12N15/70 , C12N15/87
摘要： This invention provides methods for producing and selecting host cells that better survive transformation treatment by subjecting host cells to conditions that alter them, subjecting the altered cells to transformation conditions, and selecting host cells that survive the transformation conditions. This invention also provides methods for transferring nucleic acids of interest into host cells, using cells that are better able to survive transformation treatment. Also, this invention provides kits for producing or selecting host cells in transformation treatments, as well as, kits comprising various host cells that may be utilized in transformation experiments.
摘要翻译：本发明提供了生产和选择宿主细胞的方法，所述宿主细胞通过使宿主细胞经历改变它们的条件，对改变的细胞进行转化条件以及选择在转化条件下存活的宿主细胞来转化处理更好地存活。本发明还提供了将感兴趣的核酸转移到宿主细胞中的方法，使用更好地能够转化转化处理的细胞。此外，本发明提供用于在转化治疗中产生或选择宿主细胞的试剂盒，以及包含可用于转化实验的各种宿主细胞的试剂盒。

56. 发明申请

US20040132180A1 Humanized polynucleotide sequence encoding renilla mulleri green fluorescent protein 有权
公开(公告)号：US20040132180A1
公开(公告)日：2004-07-08
申请号：US10652703
申请日：2003-08-29
申请人： Stratagene
发明人： Joseph A. Sorge , Peter Edward Vaillancourt
IPC分类号： C12N005/06 , C07H021/04
CPC分类号： G01N33/582 , C07K14/43595
摘要： The present invention provides a polynucleotide encoding a green fluorescent protein from Renilla mulleri comprising a humanized sequence which permits enhanced expression of the encoded polypeptide in mammalian cells.

57. 发明申请

US20040110267A1 Room temperature stable competent cells 审中-公开
标题翻译：室温稳定感受态细胞
公开(公告)号：US20040110267A1
公开(公告)日：2004-06-10
申请号：US10675113
申请日：2003-09-30
申请人： Stratagene
发明人： Latha Sundar
IPC分类号： C12N001/20
CPC分类号： A01N1/02 , A01N1/0221 , C12N1/04
摘要： The invention relates to methods of producing storage-stable competent cells, preparations comprising such cells, and methods of using them. More particularly, the invention relates to improved methods of generating room-temperature stable dried competent cells by exposing cells to agents and conditions that increase the survival and better maintain the competence of dried competent cells. Improvements over prior art methods are achieved by modifications of the cell growth conditions, treatments of cells before, after, or during the induction of competence, modification of competence induction methods, and modifications to drying and post-drying process steps. The invention also provides kits comprising storage-stable competent cell preparations made according to the methods of the invention.
摘要翻译：本发明涉及产生储存稳定感受态细胞的方法，包含这种细胞的制剂及其使用方法。更具体地，本发明涉及通过将细胞暴露于试剂和增加存活并且更好地维持干细胞的能力的条件来产生室温稳定的干细胞感受态细胞的改进方法。现有技术方法的改进是通过修改细胞生长条件，细胞在诱导能力之前，之后或期间的处理，能力诱导方法的修改以及对干燥和后干燥过程步骤的修改来实现的。本发明还提供了包含根据本发明方法制备的储存稳定感受态细胞制剂的试剂盒。

58. 发明申请

US20040091873A1 DNA polymerases with reduced base analog detection activity 审中-公开
标题翻译：具有降低的碱基类似物检测活性的DNA聚合酶
公开(公告)号：US20040091873A1
公开(公告)日：2004-05-13
申请号：US10298680
申请日：2002-11-18
申请人： Stratagene
发明人： Joseph A. Sorge , Holly Hogrefe , Madhushree Ghosh
IPC分类号： C12Q001/68 , C07H021/04 , C12P019/34 , C12N009/22 , C12N001/21
CPC分类号： C07H21/04 , C12N9/1252 , C12Q1/6869 , C12Q2535/101 , C12Q2521/101
摘要： The invention relates to the generation and characterization of archaeal DNA polymerase mutants with reduced base analog detection activity. The invention further provides for archaeal DNA polymerase mutants with reduced base analog detection activity containing additional mutations that modulate other DNA polymerase activities including DNA polymerization or 3null-5null exonuclease activity. The invention also discloses methods and applications of DNA polymerases with reduced base analog detection activity.
摘要翻译：本发明涉及具有降低的基础类似物检测活性的古细菌DNA聚合酶突变体的产生和表征。本发明进一步提供具有降低的碱基类似物检测活性的古细菌DNA聚合酶突变体，其含有调节其它DNA聚合酶活性（包括DNA聚合或3'-5'核酸外切酶活性）的另外的突变。本发明还公开了具有降低的碱基类似物检测活性的DNA聚合酶的方法和应用。

59. 发明申请

US20040081965A1 DNA polymerases with reduced base analog detection activity 审中-公开
标题翻译：具有降低的碱基类似物检测活性的DNA聚合酶
公开(公告)号：US20040081965A1
公开(公告)日：2004-04-29
申请号：US10280962
申请日：2002-10-25
申请人： Stratagene
发明人： Joseph A. Sorge , Holly H. Hogrefe , Madhushree Ghosh
IPC分类号： C12Q001/68 , C07H021/04 , C12P019/34 , C12N009/22 , C12N001/21
CPC分类号： C07H21/04 , C12N9/1252 , C12Q1/6869 , C12Q2535/101 , C12Q2521/101
摘要： The invention relates to the generation and characterization of archaeal DNA polymerase mutants with reduced base analog detection activity. The invention further provides for archaeal dna polymerase mutants with reduced base analog detection activity containing additional mutations that modulate other DNA polymerase activities including DNA polymerization or 3null-5null exonuclease activity. The invention also discloses methods and applications of DNA polymerases with reduced base analog detection activity.
摘要翻译：本发明涉及具有降低的基础类似物检测活性的古细菌DNA聚合酶突变体的产生和表征。本发明进一步提供具有降低的碱基类似物检测活性的古细菌dna聚合酶突变体，其含有调节其它DNA聚合酶活性（包括DNA聚合或3'-5'核酸外切酶活性）的附加突变。本发明还公开了具有降低的碱基类似物检测活性的DNA聚合酶的方法和应用。

60. 发明申请

US20040002155A1 Endonuclease VII cloning method 审中-公开
标题翻译：核酸内切酶VII克隆方法
公开(公告)号：US20040002155A1
公开(公告)日：2004-01-01
申请号：US10180174
申请日：2002-06-26
申请人： Stratagene
发明人： Alan L. Greener , Lisa Joy Hexdall , Carsten-Peter Carstens , Joseph A. Sorge
IPC分类号： C12N015/85 , C12N015/74 , C12Q001/68 , C12N001/21 , C12N005/06
CPC分类号： C12N15/10 , C12N9/22
摘要： The subject invention provides for a strain of host cells that contains a temperature sensitive variant of the gene encoding the endonuclease VII from phage T4. Using this host strain, the invention features a novel cloning method that selects for PCR products that are devoid of PCR-generated mutations.
摘要翻译：本发明提供了含有来自噬菌体T4的编码核酸内切酶VII的基因的温度敏感变体的宿主细胞菌株。使用该宿主菌株，本发明的特征在于选择缺少PCR产生突变的PCR产物的新型克隆方法。

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