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41. 发明授权

US4975715A Thermal printer having image editing function 失效
标题翻译：具有图像编辑功能的热敏打印机
公开(公告)号：US4975715A
公开(公告)日：1990-12-04
申请号：US407737
申请日：1989-09-15
申请人： Masao Saito , Tsutomu Masuda
发明人： Masao Saito , Tsutomu Masuda
IPC分类号： B41J2/325 , B41J17/12 , B41M5/26
CPC分类号： B41J17/12
摘要： A position in which an image of a second document is inserted into an image of a first document is designated by operating an operation panel. A scanner unit reads the image of the first document and a printing unit prints the read image of the first document on paper. That area of the first document image to be printed by the printing unit in which the image of the second document is inserted is masked. Paper on which the first docment image has been printed and an ink ribbon are set back to a position at which the printing operation for the first document image is started before the second document image is printed. The scanner unit reads the second document image and the printing unit prints the second document image read on a designated area of the paper on which the first document image has been printed by using ink of that portion of the ink ribbon which has not been used for printing the first document image.
摘要翻译：通过操作操作面板指定将第二文档的图像插入到第一文档的图像中的位置。扫描器单元读取第一文件的图像，并且打印单元将第一文档的读取图像打印在纸上。被插入第二文档的图像的打印单元要打印的第一文档图像的区域被遮蔽。在打印第二文档图像之前，已经打印了第一文档图像的打印纸和墨带被设置回到第一文档图像的打印操作开始的位置。扫描仪单元读取第二文档图像，并且打印单元通过使用未被使用的墨带的那部分的墨水打印在已经打印了第一文档图像的纸张的指定区域上读取的第二文档图像打印第一个文档图像。

42. 发明授权

US4968613A Luciferase gene and novel recombinant DNA as well as a method of producing luciferase 失效
标题翻译：荧光素酶基因和新型重组DNA以及荧光素酶的生产方法
公开(公告)号：US4968613A
公开(公告)日：1990-11-06
申请号：US224445
申请日：1988-07-26
申请人： Tsutomu Masuda , Hiroki Tatsumi , Eiichi Nakano
发明人： Tsutomu Masuda , Hiroki Tatsumi , Eiichi Nakano
IPC分类号： C12N9/02
CPC分类号： C12N9/0069 , C12Y113/12007
摘要： A luciferase gene isolated from Luciola cruciata (Japanese firefly) coding for an amino acid sequence shown in FIG. 4 and a novel recombinant DNA characterized by incorporating a gene coding for luciferase into a vector DNA are disclosed. There is also disclosed a method of producing luciferase which comprises culturing in a medium a microorganism containing a recombinant DNA having inserted a gene coding for luciferase in a vector DNA and belonging to the genus Escherichia capable of producing luciferase and collecting luciferase from the culture.

43. 发明授权

US4865979A Novel bacteriophage and method for breeding thereof 失效
标题翻译：新型噬菌体及其育种方法
公开(公告)号：US4865979A
公开(公告)日：1989-09-12
申请号：US498802
申请日：1983-05-27
申请人： Eiichi Nakano , Tsutomu Masuda , Yasuji Koyama , Satoshi Kitao
发明人： Eiichi Nakano , Tsutomu Masuda , Yasuji Koyama , Satoshi Kitao
IPC分类号： C12N15/09 , C12N1/21 , C12N7/00 , C12N15/00 , C12N15/70 , C12N15/73 , C12R1/91
CPC分类号： C12N15/70 , C12N15/73
摘要： A bacteriophage which is characterized by the presence of one or more restriction enzyme sites downstream from the late promoter up to the cohesive end, and none or a permissible number of restriction enzyme sites upstream from the late promoter to the cohesive end is useful as an expression vector. The vector can be used to obtain high expression of DNA inserted at the restriction enzyme site downstream from the late promoter up to the cohesive end.
摘要翻译：一种噬菌体，其特征在于存在一个或多个限制酶位点，从晚期启动子下游到粘性末端，并且没有或允许数量的限制酶位点从后期启动子上游到粘性末端可用作表达向量。该载体可用于获得插入在晚期启动子下游的限制酶位点直至粘性末端的DNA的高表达。

44. 发明授权

US4348477A Method for preparing a recombinant DNA phage 失效
标题翻译：制备重组DNA噬菌体的方法
公开(公告)号：US4348477A
公开(公告)日：1982-09-07
申请号：US87166
申请日：1979-10-22
申请人： Eiichi Nakano , Tsutomu Masuda , Narimasa Saito , Danji Fukushima
发明人： Eiichi Nakano , Tsutomu Masuda , Narimasa Saito , Danji Fukushima
IPC分类号： C12N15/09 , C12N15/00 , C12P19/34 , C12R1/91
CPC分类号： C12N15/00
摘要： A method for preparing a novel recombinant DNA, which comprises (1) cleaving with an endonuclease a phage DNA having an endonuclease-sensitive region not in the DNA segment participating in temperate phage DNA replication and integration of DNA into a host chromosome but in other DNA segments, a temperate phage DNA having an endonuclease-sensitive region in the DNA segment carrying genetic information for the production of coat protein, and a DNA carrying the intended genetic information, (2) mixing together all fragments produced by said cleaving, (3) adding DNA ligase to the mixture, and (4) recovering from the resulting mixture a phage DNA having its coat protein producing ability deleted by the replacement of the DNA segment carrying genetic information for coat protein production with a DNA fragment carrying the intended genetic information.
摘要翻译：一种制备新型重组DNA的方法，其包括（1）用核酸内切酶切割具有不在DNA片段中的内切核酸酶敏感区域的噬菌体DNA，其参与温和噬菌体DNA复制并将DNA整合到宿主染色体中，但在其他DNA （2）将由所述切割产生的所有片段混合在一起，（3）将所述切割产生的所有片段混合在一起，其中所述DNA片段具有用于产生外壳蛋白的遗传信息的DNA段中具有内切核酸酶敏感区的温带噬菌体DNA，向混合物中加入DNA连接酶，（4）从所得混合物中回收具有外壳蛋白产生能力的噬菌体DNA，通过用携带预期遗传信息的DNA片段替代携带用于外壳蛋白生产的遗传信息的DNA片段而缺失。

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