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41. 发明申请

US20050176127A1 Genes for heat resistant enzymes of amino acid biosynthetic pathway derived from thermophilic coryneform bacteria 有权
公开(公告)号：US20050176127A1
公开(公告)日：2005-08-11
申请号：US11073550
申请日：2005-03-08
申请人： Seiko Hirano , Eiichiro Kimura , Tsuyoshi Osumi , Kazuhiko Matsui , Yoshio Kawahara , Gen Nonaka , Yumi Matsuzaki , Naoki Akiyoshi , Kanae Nakamura , Osamu Kurahashi , Tsuyoshi Nakamatsu , Shinichi Sugimoto
发明人： Seiko Hirano , Eiichiro Kimura , Tsuyoshi Osumi , Kazuhiko Matsui , Yoshio Kawahara , Gen Nonaka , Yumi Matsuzaki , Naoki Akiyoshi , Kanae Nakamura , Osamu Kurahashi , Tsuyoshi Nakamatsu , Shinichi Sugimoto
IPC分类号： C07K14/34 , C12N9/02 , C12N9/04 , C12N9/12 , C12N9/26 , C12N9/88 , C12N15/31 , C12N15/53 , C12N15/54 , C12N15/56 , C12N15/60 , C12N1/21 , C12N15/74 , C12P13/08
CPC分类号： C12Y108/01004 , C07K14/34 , C12N9/0006 , C12N9/0008 , C12N9/0051 , C12N9/1205 , C12N9/2408 , C12N9/88 , C12Y101/01041 , C12Y102/01051 , C12Y102/04002 , C12Y104/01004 , C12Y203/03001 , C12Y207/01011 , C12Y302/01026 , C12Y401/01031 , C12Y401/03001 , C12Y402/01003 , C12Y604/01001 , C12Y604/01002
摘要： A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived from Corynebacterium thermoaminogenes, preferably an enzyme that functions at a higher temperature compared with that of Corynebacterium glutamicum. PCR is performed by using the primers and chromosomal DNA of Corynebacterium thermoaminogenes as a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of Corynebacterium thermoaminogenes.

42. 发明授权

US06908754B2 Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine 失效
标题翻译：棒状细菌的氨基甲酰磷酸合成酶基因及L-精氨酸的制备方法
公开(公告)号：US06908754B2
公开(公告)日：2005-06-21
申请号：US10284334
申请日：2002-10-31
申请人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
发明人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
IPC分类号： C12N1/21 , C12N9/00 , C12P13/10 , C12N9/10 , C07K17/00 , C12N9/12
CPC分类号： C12N9/93 , C12P13/10
摘要： A DNA fragment which encodes a polypeptide defined in the following (a) or (b), and a polypeptide defined in the following (c) or (d): (a) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing, (b) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a large subunit of carbamoyl-phosphate synthetase having the amino acid sequence of SEQ ID NO: 3, (c) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing, (d) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a small subunit of carbamoyl-phosphate synthetase having the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2.
摘要翻译：编码以下（a）或（b）中定义的多肽的DNA片段和以下（c）或（d）中定义的多肽：（a）至少具有氨基酸的氨基酸序列的多肽序列表所示的SEQ ID NO：2中的50〜393的酸值，（b）至少具有序列表所示的序列号2所示的氨基酸序列号为50〜393的氨基酸序列的多肽，包括取代，一个或几个氨基酸的缺失，插入，添加或倒位，并且可以构成具有氨基甲酰磷酸合成酶活性的蛋白质，其具有氨基酸序列SEQ ID NO：3的氨基甲酰磷酸合成酶的大亚基，（c ）具有序列表所示的SEQ ID NO：3的氨基酸序列的多肽，（d）具有序列表所示的SEQ ID NO：3的氨基酸序列的多肽，包括取代，缺失，插入，添加，或反转一个或几个氨基酸，并可构成具有氨基酸 - 磷酸合成酶活性的蛋白质，其具有SEQ ID NO：2中氨基酸序列号为50〜393的氨基酸序列的氨基甲酰磷酸合成酶的小亚基。

43. 发明授权

US06893852B1 Dna encoding sucrose pts enzyme II 失效
标题翻译： Dna编码蔗糖酶Ⅱ
公开(公告)号：US06893852B1
公开(公告)日：2005-05-17
申请号：US10019284
申请日：2000-06-30
申请人： Masako Izui , Masakazu Sugimoto , Tsuyoshi Nakamatsu , Osamu Kurahashi
发明人： Masako Izui , Masakazu Sugimoto , Tsuyoshi Nakamatsu , Osamu Kurahashi
IPC分类号： C12N9/12 , C12N15/54 , C07H21/04
CPC分类号： C12N9/1205 , C12Y207/01069
摘要： A gene encoding a protein constituting sucrose PTS of coryneform bacterium is provided by amplifying a region existing downstream from sucrase gene in coryneform bacterium by the cassette-ligation mediated PCR to obtain DNA encoding sucrose PTS enzyme II, which is a protein defined in the following (A) or (B): (A) a protein which has the amino acid sequence of SEQ ID NO: 2 in Sequence Listing; (B) a protein which has the amino acid sequence of SEQ ID NO: 2 in Sequence Listing including substitution, deletion, insertion, addition or inversion of one or several amino acids, and an activity for binding to sucrose.
摘要翻译：通过盒式连接介导的PCR扩增棒状杆菌型细菌的蔗糖酶基因下游的区域，得到编码构成棒状细菌的蔗糖PTS的蛋白质的基因，得到编码下述定义的蛋白质的蔗糖PTS酶II的DNA（ A）或（B）：（A）序列表中具有SEQ ID NO：2的氨基酸序列的蛋白质; （B）序列表中具有SEQ ID NO：2的氨基酸序列的蛋白质，包括一个或几个氨基酸的取代，缺失，插入，添加或转化，以及与蔗糖结合的活性。

44. 发明授权

US06303383B1 Temperature sensitive plasmid for coryneform bacteria 有权
标题翻译：棒状细菌的温度敏感质粒
公开(公告)号：US06303383B1
公开(公告)日：2001-10-16
申请号：US09521668
申请日：2000-03-08
申请人： Jun Nakamura , Sohei Kanno , Eiichiro Kimura , Kazuhiko Matsui , Tsuyoshi Nakamatsu
发明人： Jun Nakamura , Sohei Kanno , Eiichiro Kimura , Kazuhiko Matsui , Tsuyoshi Nakamatsu
IPC分类号： C12N1574
CPC分类号： C12N15/77
摘要： A coryneform bacterium in which a DNA fragment is incorporated into its chromosome is prepared by (a) obtaining a recombinant plasmid through ligation of a DNA fragment having a sequence homologous to a gene present on a chromosome of a coryneform bacterium to a plasmid that has a wild-type replication control region segment of a particular nucleotide sequence including a mutation and is autonomously replicable in a coryneform bacterium cell at a culture temperature lower than 31° C. but not autonomously replicable in the cell at a temperature of 31° C. or higher, (b) introducing the recombinant plasmid into the coryneform bacterium cell, (c) culturing the bacterium at a temperature of 31° C. or higher, (d) causing homologous recombination between the DNA fragment and the gene present on the chromosome of the coryneform bacterium and having a sequence homologous to the DNA fragment, and (e) selecting a coryneform bacterium in which the DNA fragment is incorporated into its chromosome. According to the present invention, there is provided a method for efficiently modifying genetic traits of a host in a short period of time by obtaining a temperature sensitive plasmid from a plasmid not exhibiting homology with already reported temperature sensitive plasmids or not exhibiting incompatibility therewith.
摘要翻译：通过（a）通过将具有与存在于棒状杆菌型细菌的染色体上的基因同源的序列的DNA片段连接到具有下列基因的质粒来获得重组质粒来制备将DNA片段并入其染色体中的棒状细菌：包含突变的特定核苷酸序列的野生型复制控制区段，并且在棒状细菌细胞中，在低于31℃的培养温度下是可自主复制的，但在31℃的温度下不能在细胞中自主复制，或（b）将重组质粒引入棒状细菌细胞中，（c）在31℃或更高的温度下培养细菌，（d）使DNA片段与存在于染色体上的基因之间的同源重组所述棒状细菌具有与所述DNA片段同源的序列，和（e）选择其中所述DNA片段并入其染色体中的棒状细菌 e。根据本发明，提供了一种通过从未报道的温度敏感性质粒不显示同源性的质粒或不表现出不相容性的质粒获得温度敏感性质粒来在短时间内高效地修饰宿主遗传性状的方法。

45. 发明授权

US06255086B1 Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine 有权
标题翻译：棒状细菌的氨基甲酰磷酸合成酶基因及L-精氨酸的制备方法
公开(公告)号：US06255086B1
公开(公告)日：2001-07-03
申请号：US09629616
申请日：2000-07-31
申请人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
发明人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
IPC分类号： C12P1310
CPC分类号： C12P13/10 , C12N9/93
摘要： A DNA fragment which encodes a polypeptide defined in the following (a) or (b), and a polypeptide defined in the following (c) or (d): (a) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing, (b) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID No: 2 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a large subunit of carbamoyl-phosphate synthetase having the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3, (c) a polypeptide which has the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3 shown in Sequence Listing, (d) a polypeptide which has the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a small subunit of carbamoyl-phosphate synthetase having the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2.
摘要翻译：编码以下（a）或（b）中定义的多肽的DNA片段，以及以下（c）或（d）:( a）定义的多肽，其至少具有氨基酸的氨基酸序列序列表所示的SEQ ID NO：2中的酸值为50〜393，（b）至少具有序列表所示的SEQ ID No：2的氨基酸序列号为50〜393的氨基酸序列的多肽，包括取代，一个或几个氨基酸的缺失，插入，添加或倒位，并且可以构成具有氨基甲酰基 - 磷酸合成酶活性的蛋白质，其具有氨基酸 - 磷酸合成酶的大亚基，其氨基酸序列至少包含氨基酸数55至 SEQ ID NO：3的1113，（c）具有至少包含序列表所示的SEQ ID NO：3的氨基酸编号55至1113的氨基酸序列的多肽，（d）具有氨基酸的多肽序列至少包含氨基酸序号55至1113 o f序列号3所示，包括一个或多个氨基酸的取代，缺失，插入，添加或倒位，并且可以构成具有氨基甲酰磷酸合成酶活性的氨基甲酰磷酸合成酶的小亚基的蛋白质， SEQ ID NO：2中氨基酸序列号为50〜393的氨基酸序列。

46. 发明授权

US5929221A Gene derived from coryneform bacteria and use thereof 失效
标题翻译：衍生自棒状细菌的基因及其用途
公开(公告)号：US5929221A
公开(公告)日：1999-07-27
申请号：US693228
申请日：1996-08-22
申请人： Eiichiro Kimura , Chizu Abe , Yoshio Kawahara , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
发明人： Eiichiro Kimura , Chizu Abe , Yoshio Kawahara , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
IPC分类号： C12N15/09 , C07K14/34 , C12N1/21 , C12N15/31 , C12P13/08 , C12P13/14 , C12R1/15 , C07H21/02 , C07H21/04 , C12N15/00 , C12Q1/68
CPC分类号： C12P13/14 , C07K14/34 , C12P13/08
摘要： The L-lysine productivity of an L-lysine producing Coryneform bacterium is enhanced by amplifying a novel gene derived from a Coryneform bacterium and participating in L-glutamic acid production, while the L-glutamic acid productivity of an L-glutamic acid-producing Coryneform bacterium is enhanced by suppressing the function of the gene.
摘要翻译： PCT No.PCT / JP95 / 00269 Sec。 371日期：1996年8月22日 102（e）日期1996年8月22日PCT提交1995年2月23日PCT公布。 WO95 / 23224 PCT公开号日期1995年8月31日L-赖氨酸生产棒状杆菌细菌的L-赖氨酸生产力通过扩增衍生自棒状杆菌属细菌的新基因并参与L-谷氨酸生产而增强，而L-谷氨酸生产力为L- 通过抑制基因的功能来增强产生谷氨酸的棒状细菌。

47. 发明授权

US5846790A Methods of producing L-lysine and L-glutamic acid by fermentation 失效
标题翻译：通过发酵生产L-赖氨酸和L-谷氨酸的方法
公开(公告)号：US5846790A
公开(公告)日：1998-12-08
申请号：US776597
申请日：1997-02-18
申请人： Eiichiro Kimura , Yoko Asakura , Akinori Uehara , Sumio Inoue , Yoshio Kawahara , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
发明人： Eiichiro Kimura , Yoko Asakura , Akinori Uehara , Sumio Inoue , Yoshio Kawahara , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
IPC分类号： C12N1/21 , C12N15/09 , C12P13/08 , C12P13/14
CPC分类号： C12P13/14 , C12P13/08 , Y10S435/84 , Y10S435/843
摘要： A mutant strain having an ability to produce L-glutamic acid in the absence of any biotin action-suppressing agent in a medium containing an excessive amount of biotin is obtained by giving temperature sensitivity with respect to a biotin action-suppressing agent to a coryneform L-glutamic acid-producing bacterium. This strain is cultivated in a liquid medium to produce and accumulate L-glutamic acid in the medium. A mutant strain having an ability to produce L-lysine and L-glutamic acid in the absence of any biotin action-suppressing agent in a medium containing an excessive amount of biotin is obtained by giving temperature sensitivity with respect to a biotin action-suppressing agent and giving L-lysine productivity to a coryneform L-glutamic acid-producing bacterium. This strain is cultivated in a liquid medium to simultaneously produce and accumulate L-lysine and L-glutamic acid in the medium.
摘要翻译： PCT No.PCT / JP95 / 01586 Sec。 371日期1997年2月18日 102（e）1997年2月18日PCT PCT 1995年8月9日PCT公布。出版物WO96 / 06180 日期：1996年2月29日在具有生物素活性抑制剂的情况下，在含有过量生物素的培养基中，不存在生成L-谷氨酸的能力的突变株是通过赋予生物素作用抑制代谢产L-谷氨酸棒状细菌。将该菌株在液体培养基中培养以在培养基中产生并积累L-谷氨酸。通过赋予生物素活性抑制剂的温度敏感度，获得在含有过量生物素的培养基中不存在任何生物素活性抑制剂时能够产生L-赖氨酸和L-谷氨酸的突变株并向L-谷氨酸棒状杆菌产生细菌提供L-赖氨酸生产力。该菌株在液体培养基中培养，以在培养基中同时产生和积累L-赖氨酸和L-谷氨酸。

48. 发明授权

US5429832A Feed additive composition for ruminants 失效
标题翻译：反刍动物饲料添加剂组合物
公开(公告)号：US5429832A
公开(公告)日：1995-07-04
申请号：US973621
申请日：1992-11-09
申请人： Satoshi Ueda , Haruo Heima , Makoto Ozawa , Takeshi Nagai , Tsuyoshi Nakamatsu , Hiroyuki Sato
发明人： Satoshi Ueda , Haruo Heima , Makoto Ozawa , Takeshi Nagai , Tsuyoshi Nakamatsu , Hiroyuki Sato
IPC分类号： A23K1/00 , A61K9/50 , A23K1/16 , A23K1/18 , A23L1/30
CPC分类号： A61K9/5036 , A23K40/35 , A61K9/5015
摘要： The present invention provides a feed additive composition for ruminants containing a biologically active substance therein. When administered to ruminants, the composition containing the active substance is stably protected in the rumen while efficient release of the active substance is realized in the abomasum and the following digestive tract. Thus, the released active substance is effectively digested and absorbed by ruminants.
摘要翻译：本发明提供了含有生物活性物质的反刍动物饲料添加剂组合物。当向反刍动物施用时，含有活性物质的组合物在瘤胃中被稳定地保护，同时在皱胃和随后的消化道中实现活性物质的有效释放。因此，释放的活性物质被反刍动物有效地消化和吸收。

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