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31. 发明授权

US06803219B2 Polyhydroxyalkanoate synthase and gene encoding the same enzyme 失效
标题翻译：聚羟基链烷酸酯合成酶和编码相同酶的基因
公开(公告)号：US06803219B2
公开(公告)日：2004-10-12
申请号：US10253509
申请日：2002-09-25
申请人： Tetsuya Yano , Takeshi Imamura , Sakae Suda , Tsutomu Honma
发明人： Tetsuya Yano , Takeshi Imamura , Sakae Suda , Tsutomu Honma
IPC分类号： C12N902
CPC分类号： C12N9/1025 , C12P7/625
摘要： A novel polyhydroxyalkanoate (PHA) synthase derived from a microorganism capable of producing a PHA having a novel side-chain structure and a DNA encoding the amino acid sequence for the synthase are provided. Two PHA synthase proteins (SEQ ID NOs. 1 and 3) derived from Pseudomonas cichorii YN2 (FERM BP-7375) and PHA synthase genes encoding these PHA synthases are provided, respectively (SEQ ID NOs. 2 and 4). A recombinant microorganism is endowed with a PHA producing ability.
摘要翻译：提供了源自能够产生具有新型侧链结构的PHA的微生物和编码合成酶的氨基酸序列的DNA的新型聚羟基链烷酸酯（PHA）合酶。分别提供了两种衍生自奇异假单胞菌YN2（FERM BP-7375）的PHA合成酶蛋白（SEQ ID NO.1和3）和编码这些PHA合成酶的PHA合酶基因（SEQ ID NO.2和4）。重组微生物具有PHA产生能力。

32. 发明授权

US06794168B1 Process for oxidising aromatic compounds 失效
标题翻译：氧化芳香族化合物的方法
公开(公告)号：US06794168B1
公开(公告)日：2004-09-21
申请号：US10018730
申请日：2002-04-04
申请人： Luet Lok Wong , Jonathan Peter Jones
发明人： Luet Lok Wong , Jonathan Peter Jones
IPC分类号： C12N902
CPC分类号： C12P7/22 , A01K2217/05 , A62D3/02 , A62D2101/22 , C12N9/0077
摘要： Process of oxidising a substrate which is a halo aromatic compound, which process comprises oxidizing said substrate with a monooxygenase enzyme. The enzyme may be P450cam. The process may be carried out in cells, animals or plants.
摘要翻译：氧化作为卤代芳族化合物的底物的方法，该方法包括用单加氧酶氧化所述底物。酶可以是P450cam。该方法可以在细胞，动物或植物中进行。

33. 发明授权

US06787343B2 Cytochrome P450 oxygenases and their uses 失效
公开(公告)号：US06787343B2
公开(公告)日：2004-09-07
申请号：US10142231
申请日：2002-05-08
申请人： Rodney B. Croteau , Anne Schoendorf , Stefan Jennewein
发明人： Rodney B. Croteau , Anne Schoendorf , Stefan Jennewein
IPC分类号： C12N902
CPC分类号： C12N9/0077 , A01K2217/05 , C12P17/02
摘要： Oxygenase enzymes and the use of such enzymes to produce paclitaxel (Taxol™), related taxoids, as well as intermediates in the Taxol biosynthetic pathway are disclosed. Also disclosed are nucleic acid sequences encoding the oxygenase enzymes.

34. 发明授权

US06720172B1 Genes encoding sulfate assimilation proteins 失效
公开(公告)号：US06720172B1
公开(公告)日：2004-04-13
申请号：US09720524
申请日：2000-12-21
申请人： Saverio Carl Falco
发明人： Saverio Carl Falco
IPC分类号： C12N902
CPC分类号： C12N9/1205 , C07K14/415 , C12N9/00 , C12N9/12 , C12N15/8242 , C12N15/8243 , C12N15/8253
摘要： This invention relates to an isolated nucleic acid fragment encoding a sulfate assimilation protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the sulfate assimilation protein in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the sulfate assimilation protein in a transformed host cell.

35. 发明授权

US06686186B2 Mutant fatty acid desaturase 有权
标题翻译：突变型脂肪酸去饱和酶
公开(公告)号：US06686186B2
公开(公告)日：2004-02-03
申请号：US09988929
申请日：2001-12-03
申请人： John Shanklin , Edgar B. Cahoon
发明人： John Shanklin , Edgar B. Cahoon
IPC分类号： C12N902
CPC分类号： C12N9/0083 , C12N15/8247
摘要： The present invention relates to a method for producing mutants of a fatty acid desaturase having a substantially increased activity towards fatty acid substrates with chains containing fewer than 18 carbons relative to an unmutagenized precursor desaturase having an 18 carbon atom chain length substrate specificity. The method involves inducing one or more mutations in the nucleic acid sequence encoding the precursor desaturase, transforming the mutated sequence into an unsaturated fatty acid auxotroph cell such as MH13 E. coli, culturing the cells in the absence of supplemental unsaturated fatty acids, thereby selecting for recipient cells which have received and which express a mutant fatty acid desaturase with an elevated specificity for fatty acid substrates having chain lengths of less than 18 carbon atoms. A variety of mutants having 16 or fewer carbon atom chain length substrate specificities are produced by this method. Mutant desaturases produced by this method can be introduced via expression vectors into prokaryotic and eukaryotic cells and can also be used in the production of transgenic plants which may be used to produce specific fatty acid products.
摘要翻译：本发明涉及一种生产脂肪酸去饱和酶突变体的方法，该突变体相对于具有18个碳原子链长基底特异性的未成熟前体去饱和酶，具有对具有少于18个碳的链的脂肪酸底物具有显着增加的活性。该方法包括在编码前体去饱和酶的核酸序列中诱导一个或多个突变，将突变的序列转化为不饱和脂肪酸营养缺陷型细胞如MH13大肠杆菌，在不存在补充不饱和脂肪酸的情况下培养细胞，从而选择对于接受并且表达具有链长度小于18个碳原子的脂肪酸底物具有特异性提高的突变脂肪酸去饱和酶的受体细胞。通过该方法制备具有16个或更少碳原子链长基底特异性的各种突变体。通过该方法产生的突变型去饱和酶可以通过表达载体导入原核和真核细胞中，并且还可用于生产可用于产生特定脂肪酸产物的转基因植物。

36. 发明授权

US06653115B1 D-sorbitol dehydrogenase gene 有权
公开(公告)号：US06653115B1
公开(公告)日：2003-11-25
申请号：US09635145
申请日：2000-08-08
申请人： Tatsuo Hoshino , Taro Miyazaki , Setsuko Ojima , Masako Shinjoh , Noribumi Tomiyama
发明人： Tatsuo Hoshino , Taro Miyazaki , Setsuko Ojima , Masako Shinjoh , Noribumi Tomiyama
IPC分类号： C12N902
CPC分类号： C12N9/0006 , C12P19/02
摘要： Polynucleotides encoding proteins with D-sorbitol dehydrogenase activity or which function to activate D-sorbitol dehydrogenase proteins, in vivo, vectors and recombinant organisms which contain such polynucleotides, and methods of making D-sorbitol dehydrogenase and L-sorbose.

37. 发明授权

US06613553B1 Enoyl reductases and methods of use thereof 失效
标题翻译：烯酰基还原酶及其使用方法
公开(公告)号：US06613553B1
公开(公告)日：2003-09-02
申请号：US09498520
申请日：2000-02-04
申请人： Charles O. Rock , Richard John Heath
发明人： Charles O. Rock , Richard John Heath
IPC分类号： C12N902
CPC分类号： C12N9/001
摘要： The present provides structurally related enzymes that act as enoyl reductases. These enoyl reductases share a common amino acid consensus sequence, and bind a flavin cofactor. One particular enoyl reductase provided, FabK, catalyzes the identical reaction as the NADH-dependent enoyl-ACP reductase, FabI. Nucleic acids encoding the enoyl reductases, the enoyl reductases, and anitbodies for the enoyl reductases are also included. Methods are also provides for identifying agents that can act to prevent and/or treat bacterial infections.
摘要翻译：本发明提供了用作烯酰基还原酶的结构相关的酶。这些烯酰基还原酶具有共同的氨基酸共有序列，并结合黄素辅因子。提供的一种特定的烯酰基还原酶FabK催化与NADH依赖性烯酰基-ACP还原酶FabI相同的反应。还包括编码烯酰基还原酶的核酸，烯酰基还原酶和烯酰基还原酶的活性。还提供了用于鉴定可以起到预防和/或治疗细菌感染作用的药剂的方法。

38. 发明授权

US06589767B1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids 失效
标题翻译：用于合成长链多不饱和脂肪酸的方法和组合物
公开(公告)号：US06589767B1
公开(公告)日：2003-07-08
申请号：US09377452
申请日：1999-08-19
申请人： Deborah Knutzon , Pradip Mukerji , Yung-Sheng Huang , Jennifer Thurmond , Sunita Chaudhary
发明人： Deborah Knutzon , Pradip Mukerji , Yung-Sheng Huang , Jennifer Thurmond , Sunita Chaudhary
IPC分类号： C12N902
CPC分类号： C12N9/0083 , A01K2217/05 , A23D9/00 , A23K20/158 , A23L33/12 , A23L33/40 , A23V2002/00 , A61K31/202 , A61K38/00 , C12N15/81 , C12N15/8247 , C12P7/6427 , C12P7/6472 , A23V2250/19 , A23V2250/1882 , A23V2250/1862
摘要： The present invention relates to a fatty acid &Dgr;5-desaturase able to catalyze the conversion of dihomo-gamma-linolenic acid to arachidonic acid. Nucleic acid sequences encoding a &Dgr;5-desaturase, nucleic acid sequences which hybridize thereto, DNA constructs comprising a &Dgr;5-desaturase gene, and recombinant host microorganism or animal expressing increased levels of a &Dgr;5-desaturase are described. Methods for desaturating a fatty acid at the &Dgr;5 position and for producing arachidonic acid by expressing increased levels of a &Dgr;5 desaturase are disclosed. Fatty acids, and oils containing them, which have been desaturated by a &Dgr;5-desaturase produced by recombinant host microorganisms or animals are provided. Pharmaceutical compositions, infant formulas or dietary supplements containing fatty acids which have been desaturated by a &Dgr;5-desaturase produced by a recombinant host microorganism or animal also are described.
摘要翻译：本发明涉及能够催化二高-γ-亚麻酸向花生四烯酸转化的脂肪酸DELTA5-去饱和酶。描述了编码DELTA5-去饱和酶的核酸序列，与其杂交的核酸序列，包含DELTA5-去饱和酶基因的DNA构建体和表达增加水平的DELTA5-去饱和酶的重组宿主微生物或动物。公开了通过表达增加水平的DELTA5去饱和酶在DELTA5位置使脂肪酸去饱和并产生花生四烯酸的方法。提供了通过由重组宿主微生物或动物产生的DELTA5-去饱和酶去饱和的含有它们的脂肪酸和油。还描述了由重组宿主微生物或动物产生的DELTA5-去饱和酶已经去饱和的含有脂肪酸的药物组合物，婴儿配方食品或膳食补充剂。

39. 发明授权

US06511833B1 Human fatty acid beta-oxidation enzymes 失效
公开(公告)号：US06511833B1
公开(公告)日：2003-01-28
申请号：US09481277
申请日：2000-01-11
申请人： Olga Bandman , Jennifer L. Hillman , Karl J. Guegler , Neil C. Corley , Y. Tom Tang , Purvi Shah
发明人： Olga Bandman , Jennifer L. Hillman , Karl J. Guegler , Neil C. Corley , Y. Tom Tang , Purvi Shah
IPC分类号： C12N902
CPC分类号： C12N9/001
摘要： The invention provides human fatty acid beta-oxidation enzymes (HUFA) and polynucleotides which identify and encode HUFA. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for treating or preventing disorders associated with expression of HUFA.

40. 发明授权

US06472191B1 DNA FRAGMENT CARRYING TOLUENE MONOOXYGENASE GENE, RECOMBINANT PLASMID, TRANSFORMED MICROORGANISM, METHOD FOR DEGRADING CHLORINATED ALIPHATIC HYDROCARBON COMPOUNDS AND AROMATIC COMPOUNDS, AND METHOD FOR ENVIRONMENTAL REMEDIATION 失效
标题翻译：携带TUNUENE MONOOXYGENASE基因的DNA片段，重组质粒，转化微生物，降解氯化氢化合物和芳族化合物的方法，以及环境补救方法
公开(公告)号：US06472191B1
公开(公告)日：2002-10-29
申请号：US09453956
申请日：1999-12-03
申请人： Tetsuya Yano , Tsuyoshi Nomoto , Takeshi Imamura
发明人： Tetsuya Yano , Tsuyoshi Nomoto , Takeshi Imamura
IPC分类号： C12N902
CPC分类号： C12N9/0071 , B09C1/10 , C02F3/34 , C12N9/0069 , C12P7/02
摘要： A recombinant DNA is constructed by using a toluene monooxygenase gene isolated from Ralstonia eutropha strain TB64 and employed to provide the transformant which can express toluene monooxygenase useful for cleaning of aqueous media such as drain and waste water containing halogenated aliphatic hydrocarbon compounds or aromatic compounds, for remediation of soil polluted with such compounds, and cleaning of air (gas phase) polluted with volatile organic chlorine compounds.
摘要翻译：通过使用从Ralstonia eutropha菌株TB64分离的甲苯单加氧酶基因构建重组DNA，并用于提供能够表达用于清洁含水介质如含卤化脂族烃化合物或芳族化合物的废水的甲苯单加氧酶的转化体，用于用这种化合物污染的土壤的修复以及用挥发性有机氯化合物污染的空气（气相）的清洁。

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