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31. 发明授权

US07326532B2 Detection of rpoB sequences of mycobacterium tuberculosis 有权
标题翻译：检测结核分枝杆菌rpoB序列
公开(公告)号：US07326532B2
公开(公告)日：2008-02-05
申请号：US11494963
申请日：2006-07-28
申请人： Markus T. Jucker , Steven T. Brentano , Francisco D. Delgado , Philippe Cleuziat
发明人： Markus T. Jucker , Steven T. Brentano , Francisco D. Delgado , Philippe Cleuziat
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/689 , C12Q2600/156
摘要： A method of detecting rpoB sequences of Mycobacterium tuberculosis present in a biological sample that includes steps of amplifying the M. Tuberculosis rpoB sequence in vitro in a nucleic acid amplification mixture that includes specific disclosed primer sequences, and detecting the amplified sequences by using probes that provide information by their specific hybridization to portions of the amplified nucleic acid is disclosed. Compositions for amplifying and detecting in vitro the rpoB sequences of M. Tuberculosis in a sample are disclosed.
摘要翻译：一种检测存在于生物样品中的结核分枝杆菌的rpoB序列的方法，其包括在包括特异性公开的引物序列的核酸扩增混合物中体外扩增M.Tuberculosis rpoB序列的步骤，并通过使用提供的探针来检测扩增的序列公开了通过其与部分扩增核酸的特异性杂交的信息。公开了用于在体外扩增和检测结核分枝杆菌的rpoB序列的组合物。

32. 发明授权

US06903206B1 Kits for amplifying target nucleic acid sequences using modified oligonucleotides 有权
标题翻译：使用修饰的寡核苷酸扩增靶核酸序列的试剂盒
公开(公告)号：US06903206B1
公开(公告)日：2005-06-07
申请号：US09523237
申请日：2000-03-10
申请人： Michael M. Becker , Mehrdad Majlessi , Steven T. Brentano
发明人： Michael M. Becker , Mehrdad Majlessi , Steven T. Brentano
IPC分类号： C12N15/09 , C07H21/00 , C12Q1/68 , C07H21/04
CPC分类号： C07H21/00 , C12Q1/6832 , C12Q1/686 , C12Q1/6865 , C12Q2527/107 , C12Q2525/113 , C12Q2523/113 , C12Q2537/101 , C12Q2565/107
摘要： The present invention concerns oligonucleotides containing one or more modified nucleotides which increase the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence. These modified oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence. Such modified oligonucleotides include oligonucleotides containing at least one 2′-O-methylribofuranosyl moiety joined to a nitrogenous base. Oligonucleotides can be modified in accordance with the present invention to preferentially bind RNA targets. The present invention also concerns methods of using these modified oligonucleotides and kits containing the same.
摘要翻译：本发明涉及含有一个或多个修饰的核苷酸的寡核苷酸，其增加寡核苷酸与具有互补核苷酸碱基序列的靶核酸的结合亲和力。这些修饰的寡核苷酸以比具有相同核苷酸碱基序列的未修饰寡核苷酸更快的速率与靶序列杂交。这种修饰的寡核苷酸包括含有至少一个连接到含氮碱基的2'-O-甲基呋喃核糖基部分的寡核苷酸。可以根据本发明修饰寡核苷酸以优先结合RNA靶。本发明还涉及使用这些修饰的寡核苷酸的方法和含有该寡核苷酸的试剂盒。

33. 发明授权

US06245519B1 Protection probes 有权
标题翻译：保护探头
公开(公告)号：US06245519B1
公开(公告)日：2001-06-12
申请号：US09502966
申请日：2000-02-11
申请人： Steven T. Brentano , Sherrol H. McDonough , Norman C. Nelson
发明人： Steven T. Brentano , Sherrol H. McDonough , Norman C. Nelson
IPC分类号： C12Q168
CPC分类号： C12Q1/6813 , C12Q1/6806 , C12Q1/6832 , C12Q2527/125 , C12Q2527/107 , C12Q2525/117 , C12Q2563/103 , C12Q2561/108 , C12Q2537/113 , C12Q2537/162
摘要： The present invention features compositions and methods that are useful for storing labeled detection probes and detecting whether a target nucleic acid sequence is present in a sample. Preferred compositions are made up of a detection probe containing a label susceptible to a chemical or enzymatic alteration and a protection probe that protects the label from alteration and/or decreases the ability of the detection probe to inhibit nucleic acid amplification. Such compositions can be used, for example, to stabilize a detection probe label and to prevent a detection probe from hybridizing prematurely to amplified or target nucleic acid.
摘要翻译：本发明的特征在于可用于存储标记的检测探针并检测样品中是否存在靶核酸序列的组合物和方法。优选的组合物由含有对化学或酶促改变敏感的标记的检测探针和保护标记免受改变和/或降低检测探针抑制核酸扩增能力的保护探针组成。这样的组合物可以用于例如稳定检测探针标记并防止检测探针过早与扩增或靶核酸杂交。

34. 发明申请

US20140378318A1 CIRCULARIZED TEMPLATES FOR SEQUENCING 审中-公开
标题翻译：用于测序的循环模板
公开(公告)号：US20140378318A1
公开(公告)日：2014-12-25
申请号：US14342725
申请日：2012-09-06
申请人： Steven T. Brentano , Dmitry Lyakhov , Matthew C. Friedenberg , Anne-Laure Shapiro
发明人： Steven T. Brentano , Dmitry Lyakhov , Matthew C. Friedenberg , Anne-Laure Shapiro
IPC分类号： C12Q1/68
摘要： The invention provides methods of forming a circular template for sequencing a target nucleic acid. The circular template is generated by amplification of a segment of the target nucleic acid with chimeric primers with complementary 5′ ends. The circular template has a single nick or gap providing a site for initiation of template-directed extension for sequence analysis. Sequencing of a single template generates reads of alternating segments of the same strand of the target nucleic spaced by primer segments. The different reads of the same strand of the target nucleic acid can be compiled to generate a consensus sequence. Because only one strand of the target nucleic acid is sequenced per reaction, the present method avoids errors introduced by unwittingly combining sequences of both strands of a heteroduplex PCR product. Because only one strand of the target nucleic acid is sequenced per reaction, the present method avoids errors introduced by unwittingly combining sequences of both strands of a heteroduplex PCR product.
摘要翻译：本发明提供形成用于测序靶核酸的圆形模板的方法。通过用具有互补的5'末端的嵌合引物扩增靶核酸的片段来产生圆形模板。圆形模板具有单个切口或间隙，提供用于启动用于序列分析的模板指导扩展的位点。单个模板的测序产生与引物区间隔的靶核心的相同链的交替片段的读数。可以编译目标核酸的相同链的不同读数以产生共有序列。因为每个反应只对靶核酸的一条链进行测序，所以本方法避免了通过不经意地组合异源双链PCR产物的两条链的序列引入的错误。因为每个反应只对靶核酸的一条链进行测序，所以本方法避免了通过不经意地组合异源双链PCR产物的两条链的序列引入的错误。

35. 发明授权

US08512955B2 Methods and compositions for nucleic acid amplification 有权
公开(公告)号：US08512955B2
公开(公告)日：2013-08-20
申请号：US12828676
申请日：2010-07-01
申请人： Steven T. Brentano , Dmitry Lyakhov , Norman C. Nelson , James D. Carlson , Michael M. Becker , Lyle J. Arnold, Jr.
发明人： Steven T. Brentano , Dmitry Lyakhov , Norman C. Nelson , James D. Carlson , Michael M. Becker , Lyle J. Arnold, Jr.
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C12Q1/6865 , C12Q1/6844 , C12Q1/6848 , C12Q1/6853 , C12Q2549/119 , C12Q2537/143 , C12Q2531/143
摘要： Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.

36. 发明申请

US20120178636A1 METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION 有权
标题翻译：用于核酸扩增的方法和组合物
公开(公告)号：US20120178636A1
公开(公告)日：2012-07-12
申请号：US13380018
申请日：2010-07-01
申请人： Steven T. Brentano , Dmitry Lyakhov , James D. Carlson , Michael M. Becker , Norman C. Nelson , Lyle J. Arnold, JR.
发明人： Steven T. Brentano , Dmitry Lyakhov , James D. Carlson , Michael M. Becker , Norman C. Nelson , Lyle J. Arnold, JR.
IPC分类号： C40B30/00
CPC分类号： C12Q1/6865 , C12Q1/6844 , C12Q1/6848 , C12Q1/6853 , C12Q2549/119 , C12Q2537/143 , C12Q2531/143
摘要： Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.
摘要翻译：用于进行扩增反应的组合物，反应混合物和方法，包括多重扩增反应，其中所述方法包括使用包含连接的第一和第二扩增寡聚体成员的一种或多种扩增寡聚体复合物。一方面，将扩增寡聚物复合物与靶核酸杂交，然后捕获具有杂交扩增寡聚物复合物的靶核酸，并洗涤其它组分。靶核酸的靶序列被预扩增以产生第一扩增产物。第一扩增产物在一个或多个二级扩增反应中扩增以产生第二扩增产物。

37. 发明授权

US07741027B2 Amplification of HIV-1 sequences for detection of sequences associated with drug-resistance mutations 有权
标题翻译：扩增用于检测与耐药性突变相关的序列的HIV-1序列
公开(公告)号：US07741027B2
公开(公告)日：2010-06-22
申请号：US12100350
申请日：2008-04-09
申请人： Yeasing Y. Yang , Steven T. Brentano , Odile Babola , Nathalie Tran , Guy Vernet
发明人： Yeasing Y. Yang , Steven T. Brentano , Odile Babola , Nathalie Tran , Guy Vernet
IPC分类号： C07H21/00 , C12N15/49 , C12Q1/70
CPC分类号： C07H21/02 , C12Q1/703 , H01L2924/014 , H01L2924/14 , Y10S435/974
摘要： Sequences of nucleic acid oligonucleotides for amplifying different portions of gag and pol genes of HIV-1 and for detecting such amplified nucleic acid sequences are disclosed. Methods of amplifying and detecting HIV-1 nucleic acid in a biological sample using the amplification oligonucleotides specific for gag and pol target sequences are disclosed.
摘要翻译：公开了用于扩增HIV-1的gag和pol基因的不同部分的核酸寡核苷酸的序列和用于检测这种扩增的核酸序列的序列。公开了使用特异于gag和pol靶序列的扩增寡核苷酸来扩增和检测生物样品中HIV-1核酸的方法。

38. 发明授权

US07122316B2 Method for enhancing amplification of a target nucleic acid sequence 有权
公开(公告)号：US07122316B2
公开(公告)日：2006-10-17
申请号：US10375623
申请日：2003-02-27
申请人： Christopher C. Adams , Steven T. Brentano , Gary P. Schroth
发明人： Christopher C. Adams , Steven T. Brentano , Gary P. Schroth
IPC分类号： C12Q1/68 , C12P19/34 , C01H21/02 , C07H21/04
CPC分类号： C12Q1/6865 , C07H21/00 , C12Q1/6806 , C12Q2525/301 , C12Q2549/125
摘要： The present invention features inhibitors of target-independent amplification and the use of such inhibitors for enhancing an amplification protocol. The inhibitors are believed to enhance an amplification protocol by inhibiting the ability of one or more nucleic acid polymerases to use nucleic acid in a polymerase reaction in the absence of target nucleic acid.

39. 发明授权

US06747141B2 Methods and compositions for detection of mycobacterium avium complex species 有权
标题翻译：用于检测鸟分枝杆菌复合物种的方法和组合物
公开(公告)号：US06747141B2
公开(公告)日：2004-06-08
申请号：US09738972
申请日：2000-12-15
申请人： Steven T. Brentano , Roger L. Lankford
发明人： Steven T. Brentano , Roger L. Lankford
IPC分类号： C12Q168
CPC分类号： C12Q1/689
摘要： Methods of detecting Mycobacterium avium complex (MAC) organisms using in vitro nucleic acid amplification with amplification oligonucleotides specific for 16S rRNA or DNA sequences encoding 16S rRNA from MAC species are disclosed. Compositions and kits containing oligonucleotides for amplifying and detecting 16S rRNA or DNA sequences encoding 16S rRNA from MAC species are disclosed.
摘要翻译：公开了使用具有16S rRNA特异性的扩增寡核苷酸或编码来自MAC物种的16S rRNA的DNA序列的体外核酸扩增来检测鸟分枝杆菌复合物（MAC）生物体的方法。公开了含有用于扩增和检测来自MAC物种的16S rRNA的16S rRNA或DNA序列的寡核苷酸的组合物和试剂盒。

40. 发明授权

US10752944B2 Circularized templates for sequencing 有权
公开(公告)号：US10752944B2
公开(公告)日：2020-08-25
申请号：US14342725
申请日：2012-09-06
申请人： Steven T. Brentano , Dmitry Lyakhov , Matthew C. Friedenberg , Anne-Laure Shapiro
发明人： Steven T. Brentano , Dmitry Lyakhov , Matthew C. Friedenberg , Anne-Laure Shapiro
IPC分类号： C12Q1/68 , C12Q1/6869 , C12Q1/6855 , C12Q1/6844
摘要： The invention provides methods of forming a circular template for sequencing a target nucleic acid. The circular template is generated by amplification of a segment of the target nucleic acid with chimeric primers with complementary 5′ ends. The circular template has a single nick or gap providing a site for initiation of template-directed extension for sequence analysis. Sequencing of a single template generates reads of alternating segments of the same strand of the target nucleic spaced by primer segments. The different reads of the same strand of the target nucleic acid can be compiled to generate a consensus sequence. Because only one strand of the target nucleic acid is sequenced per reaction, the present method avoids errors introduced by unwittingly combining sequences of both strands of a heteroduplex PCR product. Because only one strand of the target nucleic acid is sequenced per reaction, the present method avoids errors introduced by unwittingly combining sequences of both strands of a heteroduplex PCR product.

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