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1. 发明授权

US10752944B2 Circularized templates for sequencing 有权
公开(公告)号：US10752944B2
公开(公告)日：2020-08-25
申请号：US14342725
申请日：2012-09-06
申请人： Steven T. Brentano , Dmitry Lyakhov , Matthew C. Friedenberg , Anne-Laure Shapiro
发明人： Steven T. Brentano , Dmitry Lyakhov , Matthew C. Friedenberg , Anne-Laure Shapiro
IPC分类号： C12Q1/68 , C12Q1/6869 , C12Q1/6855 , C12Q1/6844
摘要： The invention provides methods of forming a circular template for sequencing a target nucleic acid. The circular template is generated by amplification of a segment of the target nucleic acid with chimeric primers with complementary 5′ ends. The circular template has a single nick or gap providing a site for initiation of template-directed extension for sequence analysis. Sequencing of a single template generates reads of alternating segments of the same strand of the target nucleic spaced by primer segments. The different reads of the same strand of the target nucleic acid can be compiled to generate a consensus sequence. Because only one strand of the target nucleic acid is sequenced per reaction, the present method avoids errors introduced by unwittingly combining sequences of both strands of a heteroduplex PCR product. Because only one strand of the target nucleic acid is sequenced per reaction, the present method avoids errors introduced by unwittingly combining sequences of both strands of a heteroduplex PCR product.

2. 发明授权

US10767208B2 Closed nucleic acid structures 有权
公开(公告)号：US10767208B2
公开(公告)日：2020-09-08
申请号：US14342764
申请日：2012-09-06
申请人： Norman C. Nelson , Jijumon Chelliserry , Steven T. Brentano , Dmitry Lyakhov , Matthew C. Friedenberg , Anne-Laure Shapiro
发明人： Norman C. Nelson , Jijumon Chelliserry , Steven T. Brentano , Dmitry Lyakhov , Matthew C. Friedenberg , Anne-Laure Shapiro
IPC分类号： C12Q1/68 , C12P19/34 , C12Q1/6869 , C12Q1/6855
摘要： The invention provides compositions and methods for making closed nucleic acid structures in which one or both strands are continuous. The closed nucleic acid structures can be used as sequencing templates among other applications.

3. 发明申请

US20140329282A1 CLOSED NUCLEIC ACID STRUCTURES 审中-公开
标题翻译：封闭的核酸结构
公开(公告)号：US20140329282A1
公开(公告)日：2014-11-06
申请号：US14342764
申请日：2012-09-06
申请人： Norman C. Nelson , Jijumon Chelliserry , Steven T. Brentano , Dmitry Lyakhov , Matthew C. Friedenberg , Anne-Laure Shapiro
发明人： Norman C. Nelson , Jijumon Chelliserry , Steven T. Brentano , Dmitry Lyakhov , Matthew C. Friedenberg , Anne-Laure Shapiro
IPC分类号： C12P19/34
摘要： The invention provides compositions and methods for making closed nucleic acid structures in which one or both strands are continuous. The closed nucleic acid structures can be used as sequencing templates among other applications.
摘要翻译：本发明提供用于制备其中一条或两条链是连续的封闭核酸结构的组合物和方法。封闭的核酸结构可以用作其他应用中的测序模板。

4. 发明申请

US20140378318A1 CIRCULARIZED TEMPLATES FOR SEQUENCING 审中-公开
标题翻译：用于测序的循环模板
公开(公告)号：US20140378318A1
公开(公告)日：2014-12-25
申请号：US14342725
申请日：2012-09-06
申请人： Steven T. Brentano , Dmitry Lyakhov , Matthew C. Friedenberg , Anne-Laure Shapiro
发明人： Steven T. Brentano , Dmitry Lyakhov , Matthew C. Friedenberg , Anne-Laure Shapiro
IPC分类号： C12Q1/68
摘要： The invention provides methods of forming a circular template for sequencing a target nucleic acid. The circular template is generated by amplification of a segment of the target nucleic acid with chimeric primers with complementary 5′ ends. The circular template has a single nick or gap providing a site for initiation of template-directed extension for sequence analysis. Sequencing of a single template generates reads of alternating segments of the same strand of the target nucleic spaced by primer segments. The different reads of the same strand of the target nucleic acid can be compiled to generate a consensus sequence. Because only one strand of the target nucleic acid is sequenced per reaction, the present method avoids errors introduced by unwittingly combining sequences of both strands of a heteroduplex PCR product. Because only one strand of the target nucleic acid is sequenced per reaction, the present method avoids errors introduced by unwittingly combining sequences of both strands of a heteroduplex PCR product.
摘要翻译：本发明提供形成用于测序靶核酸的圆形模板的方法。通过用具有互补的5'末端的嵌合引物扩增靶核酸的片段来产生圆形模板。圆形模板具有单个切口或间隙，提供用于启动用于序列分析的模板指导扩展的位点。单个模板的测序产生与引物区间隔的靶核心的相同链的交替片段的读数。可以编译目标核酸的相同链的不同读数以产生共有序列。因为每个反应只对靶核酸的一条链进行测序，所以本方法避免了通过不经意地组合异源双链PCR产物的两条链的序列引入的错误。因为每个反应只对靶核酸的一条链进行测序，所以本方法避免了通过不经意地组合异源双链PCR产物的两条链的序列引入的错误。

5. 发明授权

US06902891B2 Process for labeling a nucleic acid 有权
标题翻译：核酸标记方法
公开(公告)号：US06902891B2
公开(公告)日：2005-06-07
申请号：US09736151
申请日：2000-12-15
申请人： Ali Laayoun , Lionel Menou , Christelle Tora , Aloke R. Banerjee , Michael M. Becker , Kenneth A. Browne , Matthew C. Friedenberg , Fred F. Hajjar
发明人： Ali Laayoun , Lionel Menou , Christelle Tora , Aloke R. Banerjee , Michael M. Becker , Kenneth A. Browne , Matthew C. Friedenberg , Fred F. Hajjar
IPC分类号： C07H21/00 , C12Q1/68 , C07D225/00 , C07D259/00 , C07H21/04 , C12P19/34
CPC分类号： C07H21/00 , C12Q2523/107
摘要： A process of fragmenting and labeling a synthetic or natural nucleic acid, comprising the steps of providing a mixture containing a nucleic acid, a labeling agent containing a detectable label, and at least one multivalent metal cation in a substantially aqueous solution; chemically fragmenting the nucleic acid in the mixture to produce a multiplicity of nucleic acid fragments; and attaching at least one label to at least one of the nucleic acid fragments to produce a detectably labeled nucleic acid fragment.
摘要翻译：包括以下步骤：提供含有核酸，含有可检测标记的标记试剂和至少一种多价金属阳离子在基本水溶液中的混合物的步骤; 将混合物中的核酸化学碎裂以产生多种核酸片段; 以及将至少一个标记附着到至少一个所述核酸片段以产生可检测地标记的核酸片段。

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