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31. 发明授权

US4409327A Process and reagent for the determination of the biological activity of heparin in plasma 失效
标题翻译：用于测定血浆中肝素生物活性的方法和试剂
公开(公告)号：US4409327A
公开(公告)日：1983-10-11
申请号：US233536
申请日：1981-02-11
申请人： Knut Bartl , Helmut Lill , Peter Roeschlau , Joachim Ziegenhorn
发明人： Knut Bartl , Helmut Lill , Peter Roeschlau , Joachim Ziegenhorn
IPC分类号： G01N33/66 , C12Q1/37 , C12Q1/56 , G01N33/50 , G01N33/86 , C12Q1/38
CPC分类号： G01N33/86 , C12Q2337/12 , G01N2333/8117 , G01N2333/96444 , G01N2333/974 , G01N2400/40 , Y10S435/81
摘要： Method and reagent for the determination of the biological activity of heparin in plasma by adding thrombin or Factor X.sub.a as the proteolytic enzyme and a chromogen substrate of the latter and measuring the dye liberated from the chromogen substrate in the absence of exogenous anti-thrombin III, the improvement consisting of adding, additionally, at least one compound of the formula ##STR1## wherein X is oxygen or the group NR.sub.5 ;and R.sub.1, R.sub.2, R.sub.3, R.sub.4 and R.sub.5 are independently selected from hydrogen or alkyl of 1 to 2 carbon atoms.
摘要翻译：通过添加凝血酶或因子Xa作为蛋白水解酶和后者的色底物，测定血浆中肝素生物活性的方法和试剂，并测定在没有外源性抗凝血酶III条件下从色底物释放的染料，该改进包括另外加入至少一种式（IMAGE）化合物，其中X是氧或NR5基团; 并且R 1，R 2，R 3，R 4和R 5独立地选自氢或1至2个碳原子的烷基。

32. 发明授权

US4301028A Control reagent for heparin activity determination 失效
标题翻译：用于肝素活性测定的对照试剂
公开(公告)号：US4301028A
公开(公告)日：1981-11-17
申请号：US110581
申请日：1980-01-09
申请人： Knut Bartl , Joachim Ziegenhorn , Peter Wunderwald , Klaus Beaucamp , Helmut Lill
发明人： Knut Bartl , Joachim Ziegenhorn , Peter Wunderwald , Klaus Beaucamp , Helmut Lill
IPC分类号： G01N33/66 , G01N33/50 , G01N33/86 , G01N33/96 , G01N33/16 , C09K3/00
CPC分类号： G01N33/96 , G01N2400/40 , G01N33/86 , Y10T436/10 , Y10T436/106664
摘要： A control reagent for heparin activity determination comprising20 to 1500 USP heparin,2000 to 30000 IU antithrombin III,0.15 to 1.5 millimoles serum albumin,0.005 to 0.05 mole ethylenediaminetetraacetic acid sodium salt,0 to 0.4 mole saccharose,2000 to 20000 U aprotinin, andbuffer substance, pH 6.5 to 7.5,per liter of control reagent solution.
摘要翻译：用于肝素活性测定的对照试剂，其包含20至1500USB肝素，2000至30000IU抗凝血酶III，0.15至1.5毫摩尔血清白蛋白，0.005至0.05摩尔乙二胺四乙酸钠盐，0至0.4摩尔蔗糖，2000至20000U抑肽酶，和缓冲物质，pH 6.5〜7.5，每升对照试剂溶液。

33. 发明授权

US4868139A Aqueous cholesterol standard solution and process for its preparation 失效
标题翻译：水性胆固醇标准溶液及其制备方法
公开(公告)号：US4868139A
公开(公告)日：1989-09-19
申请号：US122019
申请日：1987-11-17
申请人： Rolf Deeg , Gisela Dengler , Joachim Ziegenhorn
发明人： Rolf Deeg , Gisela Dengler , Joachim Ziegenhorn
IPC分类号： G01N33/50 , C12Q1/60 , G01N33/92
CPC分类号： C12Q1/60 , Y10T436/104165 , Y10T436/108331
摘要： The present invention provides an aqueous cholesterol standard solution with a definite content of cholesterol, wherein it contains a detergent mixture of 10 to 90% of cholic acid and 90 to 10% of desoxycholic acid or of appropriate salts or derivatives of these acids.The present invention also provides a process for the preparation of this aqueous cholesterol standard solution, wherein a detergent mixture of cholic acid and desoxycholic acid or of appropriate salts and derivatives of these acids is dissolved in distilled water or in 0.9% aqueous sodium chloride solution, an appropriate preservation agent and/or a buffer effective in the pH range of from 7 to 9 optionally added thereto, and a definite, precisely defined amount of cholesterol is dissolved in the solution thus obtained, while stirring and warming to 40.degree. to 60.degree. C.
摘要翻译：本发明提供具有一定胆固醇含量的胆固醇水溶液，其中含有10-90％胆酸和90-10％脱氧胆酸或这些酸的适当盐或衍生物的洗涤剂混合物。本发明还提供了制备这种胆固醇水溶液标准溶液的方法，其中将胆酸和脱氧胆酸的洗涤剂混合物或这些酸的适当盐和衍生物溶解在蒸馏水中或在0.9％氯化钠水溶液中，适当的保存剂和/或在任意添加的pH范围为7〜9的有效缓冲液中，将一定精确定量的胆固醇溶于所得溶液中，同时搅拌并升温至40〜60℃ C。

34. 发明授权

US4746605A Process and a reagent for the determination of low density lipoproteins (LDL) 失效
标题翻译：用于测定低密度脂蛋白（LDL）的方法和试剂
公开(公告)号：US4746605A
公开(公告)日：1988-05-24
申请号：US656954
申请日：1984-10-02
申请人： Lorenz Kerscher , Joachim Ziegenhorn , Sigbert Schiefer
发明人： Lorenz Kerscher , Joachim Ziegenhorn , Sigbert Schiefer
IPC分类号： G01N33/53 , C12Q1/00 , C12Q1/60 , G01N33/531 , G01N33/536 , G01N33/68 , G01N33/92
CPC分类号： C12Q1/60 , G01N33/92 , G01N2800/044 , Y10S435/962 , Y10S436/817 , Y10S436/825 , Y10S436/826 , Y10T436/25125
摘要： A method for determining low density lipoproteins (LDLs) in a body fluid sample, as well as a reagent suited for this use, are taught. The method involves precipitating high density lipoproteins (HDLs) from the sample, using an HDL specific antibody or reactive fragment, and then determining the presence and amount of LDL in the supernatant which results. The reagent contains the HDL specific antibodies, as well as polyanions and divalent cations.
摘要翻译：教导了体液样品中低密度脂蛋白（LDL）的测定方法，以及适用于此用途的试剂。该方法包括使用HDL特异性抗体或活性片段从样品中沉淀高密度脂蛋白（HDL），然后测定上清液中LDL的存在和量。试剂含有HDL特异性抗体，以及聚阴离子和二价阳离子。

35. 发明授权

US4705749A Process and reagent for the determination of glycerol with the use of glycerol kinase 失效
标题翻译：使用甘油激酶测定甘油的方法和试剂
公开(公告)号：US4705749A
公开(公告)日：1987-11-10
申请号：US667279
申请日：1984-11-01
申请人： Peter Willnow , Paul Lehmann , Joachim Ziegenhorn , August W. Wahlefeld
发明人： Peter Willnow , Paul Lehmann , Joachim Ziegenhorn , August W. Wahlefeld
IPC分类号： C12Q1/28 , C12Q1/44 , C12Q1/48 , C12N9/99 , C12Q1/26 , C12Q1/32
CPC分类号： C12Q1/44 , C12Q1/28 , C12Q1/48 , C12Q2326/96 , C12Q2334/00 , G01N2333/904 , G01N2333/9121 , Y10S435/805 , Y10S435/81
摘要： The present invention provides a process for the determination of glycerol in free or bound form by reaction with ATP in the presence of glycerol kinase (GK) and optionally of a hydrolase with the formation of glycerol-3-phosphate and ADP and determination of one of these reaction products with the help of at least one subsequent enzymatic reaction, wherein the determination is carried out kinetically and, for this purpose, the reaction with ATP is made rate-determining for the whole reaction and this is allowed to proceed according to the pseudo-first order in that there is added a sugar of the general formula: ##STR1## in which carbon atoms 2 and 3 have the D-threo configuration and R is a carbohydrate radical containing up to 3 carbon atoms and in which one hydroxyl group can also be replaced by a hydrogen atom.The present invention also provides a reagent for the kinetic determination of glycerol, comprising glycerol kinase, ATP and a system for the determination of glycerol-3-phosphate or of ADP and optionally a hydrolase, wherein it additionally contains a sugar of the general formula: ##STR2## in which carbon atoms 2 and 3 have the D-threo configuration and R is a carbohydrate radical containing up to 3 carbon atoms and in which one hydroxyl group can also be replaced by a hydrogen atom.
摘要翻译：本发明提供了一种通过在甘油激酶（GK）存在下与ATP反应来测定游离或结合形式的甘油的方法，并且任选地形成甘油-3-磷酸酯和ADP的水解酶，并测定其中之一这些反应产物借助于至少一个随后的酶反应，其中所述测定在动力学上进行，为此，与ATP的反应对整个反应进行速率确定，并且允许其根据伪添加了具有以下通式的糖：其中碳原子2和3具有D-苏糖构型并且R是含有至多3个碳原子的碳水化合物基团，其中一个羟基也可以被氢原子代替。本发明还提供用于动力学测定甘油的试剂，其包含甘油激酶，ATP和用于测定甘油-3-磷酸或ADP和任选的水解酶的系统，其中其还含有以下通式的糖：其中碳原子2和3具有D-苏结构，并且R是含有至多3个碳原子的碳水化合物基团，其中一个羟基也可被氢原子代替。

36. 发明授权

US4486531A Method and reagent for the determination of .beta.-lipoprotein 失效
标题翻译：用于测定β-脂蛋白的方法和试剂
公开(公告)号：US4486531A
公开(公告)日：1984-12-04
申请号：US489139
申请日：1983-05-04
申请人： Joachim Ziegenhorn , Knut Bartl , Max Brandhuber
发明人： Joachim Ziegenhorn , Knut Bartl , Max Brandhuber
IPC分类号： G01N33/68 , C12Q1/44 , G01N33/92 , C12Q1/60
CPC分类号： C12Q1/44 , G01N33/92 , G01N2800/044 , Y10S435/81 , Y10T436/104165 , Y10T436/105831 , Y10T436/107497 , Y10T436/25125 , Y10T436/25375
摘要： The present invention provides a process for the direct turbidimetric determination of .beta.-lipoproteins (LDL) in body fluids by precipitation with polyanions and divalent cations, wherein the precipitation is carried out in the presence of a complex former at a pH value of from 7 to 9.The present invention also provides a reagent for the direct turbidimetric determination of .beta.-lipoproteins (LDL) in body fluids, comprising a polyanion, a salt of a divalent metal, a complex former and a substance buffering at pH 7 to 9.
摘要翻译：本发明提供一种通过用聚阴离子和二价阳离子沉淀直接比浊测定体液中的β-脂蛋白（LDL）的方法，其中沉淀是在复合物的存在下在pH值为7至本发明还提供用于直接比浊测定体液中的β-脂蛋白（LDL）的试剂，其包含聚阴离子，二价金属的盐，复合物和在pH7至9下缓冲的物质。

37. 发明授权

US5407836A Process and reagent for the determination of low density lipoproteins (LDL) 失效
标题翻译：用于测定低密度脂蛋白（LDL）的方法和试剂
公开(公告)号：US5407836A
公开(公告)日：1995-04-18
申请号：US85456
申请日：1993-06-30
申请人： Joachim Ziegenhorn , Sigbert Schiefer , Brigitte Drager
发明人： Joachim Ziegenhorn , Sigbert Schiefer , Brigitte Drager
IPC分类号： G01N33/53 , C12Q1/26 , C12Q1/28 , C12Q1/44 , C12Q1/60 , G01N33/92 , G01N33/539 , G01N33/543
CPC分类号： C12Q1/60 , G01N33/92 , G01N2800/044 , Y10S435/962 , Y10S436/815
摘要： The present invention provides a process for the determination of low density lipoproteins (LDL) in body fluids, wherein high density lipoprotein (HDL) antibodies are added to a sample to be investigated, insolubles formed are separated off and the LDL or one of its components is determined in the supernatant.The present invention also provides a reagent for the determination of the LDL fraction in body fluids, wherein it contains HDL antibodies.
摘要翻译：本发明提供了一种用于测定体液中的低密度脂蛋白（LDL）的方法，其中将高密度脂蛋白（HDL）抗体添加到待研究的样品中，形成的不溶物被分离出来，LDL或其组分之一在上清液中测定。本发明还提供了用于测定体液中LDL分数的试剂，其中它含有HDL抗体。

38. 发明授权

US4695552A Process and reagent for the determination of the hemoglobin-haptoglobin complex in the presence of free hemoglobin 失效
标题翻译：在游离血红蛋白存在下测定血红蛋白 - 触珠蛋白复合物的方法和试剂
公开(公告)号：US4695552A
公开(公告)日：1987-09-22
申请号：US601801
申请日：1984-04-19
申请人： Urban Schmitt , Rolf Deeg , Joachim Ziegenhorn
发明人： Urban Schmitt , Rolf Deeg , Joachim Ziegenhorn
IPC分类号： C12Q1/28 , G01N33/72
CPC分类号： C12Q1/28 , G01N33/725 , C12Q2326/30 , Y10T436/25125
摘要： The present invention provides a process for the determination of the hemoglobin-haptoglobin complex in the presence of free hemoglobin by utilization of the different peroxidate properties of free and of bound hemoglobin, wherein, for the selective inhibition of the peroxidase activity of the free hemoglobin, a detergent is added and the residual peroxidate activity of the reaction mixture is measured.The present invention also provides a reagent for carrying out this process wherein, besides the substances required for the determination of the peroxidase activity, it contains a detergent for the inhibition of the peroxidase activity of free hemoglobin.Furthermore, the present invention provides a process for determining the haptoglobin content of a sample, as well as a process for determining glycosilated hemoglobin in a sample.
摘要翻译：本发明提供了一种通过利用游离血红蛋白和结合血红蛋白的不同过氧化物特性在游离血红蛋白存在下测定血红蛋白 - 触珠蛋白复合物的方法，其中，对于选择性抑制游离血红蛋白的过氧化物酶活性，添加洗涤剂，并测量反应混合物的过氧化物的残留活性。本发明还提供了一种用于进行该方法的试剂，其中除了确定过氧化物酶活性所需的物质外，还含有用于抑制游离血红蛋白的过氧化物酶活性的洗涤剂。此外，本发明提供了测定样品的触珠蛋白含量的方法，以及测定样品中糖基化血红蛋白的方法。

39. 发明授权

US4521519A Reagent for the precipitation of apo-B-containing lipoproteins 失效
标题翻译：含有载脂蛋白B的脂蛋白的沉淀试剂
公开(公告)号：US4521519A
公开(公告)日：1985-06-04
申请号：US369382
申请日：1982-04-19
申请人： Brigitte Draeger , Joachim Ziegenhorn
发明人： Brigitte Draeger , Joachim Ziegenhorn
IPC分类号： G01N33/68 , G01N33/92
CPC分类号： G01N33/92 , G01N2800/044 , Y10S436/825 , Y10T436/107497 , Y10T436/25
摘要： The present invention provides a reagent for the precipitation of apo-B-containing lipoproteins, wherein it comprises 0.2 to 3 grams per liter of phosphotungstic acid and more than 2 mmols per liter of magnesium ions in aqueous solution.The present invention also provides a process for the preparation of this reagent and a process for the precipitation of apo-B-containing lipoproteins using this reagent.
摘要翻译：本发明提供了用于沉淀含有载脂蛋白B的脂蛋白的试剂，其中它包含0.2-3克/升磷钨酸和大于2毫摩尔/升的镁离子在水溶液中。本发明还提供了制备该试剂的方法和使用该试剂沉淀含有载脂蛋白B的脂蛋白的方法。

40. 发明授权

US4229527A Process and reagent for the kinetic determination of enzyme substrates 失效
标题翻译：用于酶底物动力学测定的方法和试剂
公开(公告)号：US4229527A
公开(公告)日：1980-10-21
申请号：US954139
申请日：1978-10-23
申请人： Joachim Ziegenhorn , August W. Wahlefeld , Alexander Hagen , Wolfgang Gruaber , Hans U. Bergmeyer
发明人： Joachim Ziegenhorn , August W. Wahlefeld , Alexander Hagen , Wolfgang Gruaber , Hans U. Bergmeyer
IPC分类号： G01N33/50 , C12Q1/00 , C12Q1/54 , C12Q1/60 , C12Q1/61 , G01N21/75 , G01N33/48 , G01N33/66 , G01N33/92 , C12Q1/50 , C12Q1/44 , C12Q1/48
CPC分类号： C12Q1/61 , C12Q1/00 , C12Q1/54 , C12Q1/60 , Y10S435/81
摘要： Kinetic determination of enzyme substrates by means of coupled reactions comprising selecting the reaction parameters so that the most specific part reaction of a reaction sequence becomes rate-determining for the whole reaction sequence wherein the rate-determining part reaction follows first or pseudo-first order kinetics.
摘要翻译：通过偶联反应动力学测定酶底物，包括选择反应参数，使得反应序列的最具体的部分反应成为整个反应顺序的速率确定，其中速率确定部分反应遵循第一或伪一级动力学。

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