专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US4438204A Determination of glycosilated hemoglobin 失效
标题翻译：测定糖化血红蛋白
公开(公告)号：US4438204A
公开(公告)日：1984-03-20
申请号：US426510
申请日：1982-09-29
申请人： Rolf Deeg , Urban Schmitt , Joachim Ziegenhorn
发明人： Rolf Deeg , Urban Schmitt , Joachim Ziegenhorn
IPC分类号： G01N33/52 , G01N21/78 , G01N30/88 , G01N33/53 , G01N33/66 , G01N33/72
CPC分类号： G01N33/723 , Y10T436/107497
摘要： A process for the determination of glycosilated hemoglobin in a blood sample comprises liberating glycosilated and non-glycosilated hemoglobin from the erythrocytes by chemical or physical treatment; converting, if desired, the hemoglobin into methemoglobin, and thereafter differentiating the glycosilated and non-glycosilated hemoglobin portion using its reaction with haptoglobin, and determining the glycosilated and/or the non-glycosilated hemoglobin portion.The present invention also provides a reagent for the determination of glycosilated hemoglobin in a blood sample, as well as a reagent for the differentiation of glycosilated and non-glycosilated hemoglobin, comprising haptoglobin.
摘要翻译：用于测定血液样品中糖基化血红蛋白的方法包括通过化学或物理处理从红细胞释放糖基化和非糖基化血红蛋白; 如果需要，将血红蛋白转化成高铁血红蛋白，然后使用其与触珠蛋白的反应来分化糖基化和非糖基化血红蛋白部分，并测定糖基化和/或非糖基化血红蛋白部分。本发明还提供了用于测定血液样品中的糖基化血红蛋白的试剂，以及包含触珠蛋白的糖基化和非糖化血红蛋白的分化试剂。

2. 发明授权

US4695552A Process and reagent for the determination of the hemoglobin-haptoglobin complex in the presence of free hemoglobin 失效
标题翻译：在游离血红蛋白存在下测定血红蛋白 - 触珠蛋白复合物的方法和试剂
公开(公告)号：US4695552A
公开(公告)日：1987-09-22
申请号：US601801
申请日：1984-04-19
申请人： Urban Schmitt , Rolf Deeg , Joachim Ziegenhorn
发明人： Urban Schmitt , Rolf Deeg , Joachim Ziegenhorn
IPC分类号： C12Q1/28 , G01N33/72
CPC分类号： C12Q1/28 , G01N33/725 , C12Q2326/30 , Y10T436/25125
摘要： The present invention provides a process for the determination of the hemoglobin-haptoglobin complex in the presence of free hemoglobin by utilization of the different peroxidate properties of free and of bound hemoglobin, wherein, for the selective inhibition of the peroxidase activity of the free hemoglobin, a detergent is added and the residual peroxidate activity of the reaction mixture is measured.The present invention also provides a reagent for carrying out this process wherein, besides the substances required for the determination of the peroxidase activity, it contains a detergent for the inhibition of the peroxidase activity of free hemoglobin.Furthermore, the present invention provides a process for determining the haptoglobin content of a sample, as well as a process for determining glycosilated hemoglobin in a sample.
摘要翻译：本发明提供了一种通过利用游离血红蛋白和结合血红蛋白的不同过氧化物特性在游离血红蛋白存在下测定血红蛋白 - 触珠蛋白复合物的方法，其中，对于选择性抑制游离血红蛋白的过氧化物酶活性，添加洗涤剂，并测量反应混合物的过氧化物的残留活性。本发明还提供了一种用于进行该方法的试剂，其中除了确定过氧化物酶活性所需的物质外，还含有用于抑制游离血红蛋白的过氧化物酶活性的洗涤剂。此外，本发明提供了测定样品的触珠蛋白含量的方法，以及测定样品中糖基化血红蛋白的方法。

3. 发明授权

US4816393A Nucleoside triphosphate-dependent 1-methylhydantoinase, a process for obtaining it and the use thereof 失效
标题翻译：核苷三磷酸依赖性1-甲基乙内酰脲酶，其获得方法及其用途
公开(公告)号：US4816393A
公开(公告)日：1989-03-28
申请号：US704712
申请日：1985-02-25
申请人： Joachim Siedel , Rolf Deeg , Albert Roder , Joachim Ziegenhorn , Hans Mollering , Helmgard Gauhl
发明人： Joachim Siedel , Rolf Deeg , Albert Roder , Joachim Ziegenhorn , Hans Mollering , Helmgard Gauhl
IPC分类号： C12N9/14 , C12N9/86 , C12Q1/34 , C12R1/01 , C12R1/06 , C12R1/13 , C12R1/265 , G01N33/70 , C12Q1/26 , C12Q1/32
CPC分类号： C12N9/86 , C12Q1/34 , G01N33/70 , G01N2333/986 , Y10S435/81
摘要： The present invention provides 1-methylhydantoinase, which hydrolyses 1-methylhydantoin in the presence of a nucleoside triphosphate and of polyvalent metal ions.The present invention also provides a process for obtaining 1-methylhydantoinase and a reagent containing it.Furthermore, the present invention provides a process for the determination of creatinine by the conversion of the creatinine with creatinine deiminase (E.C. 3.5.4.21) into 1-methylhydantoin, hydrolysis of the latter with the 1-methylhydantoinase in the presence of nucleoside triphosphate and of polyvalent metal ions and determination(a) of the hydrolysis product formed from 1-methylhydantoin with N-carbamoylsarcosinamidohydrolase with formation of sarcosine and detection of the sarcosine with sarcosine oxidase or sarcosine dehydrogenase or(b) of the simultaneously formed nucleoside diphosphate.
摘要翻译：本发明提供1-甲基乙内酰脲酶，其在核苷三磷酸和多价金属离子的存在下水解1-甲基乙内酰脲。本发明还提供了获得1-甲基乙内酰脲酶和含有它的试剂的方法。此外，本发明提供了通过将肌酐与肌酐去酰亚胺酶（EC 3.5.4.21）转化为1-甲基乙内酰脲来测定肌酸酐的方法，后者在核苷三磷酸存在下用1-甲基乙内酰脲酶水解，测定（a）由N-甲基乙内酰脲与N-氨基甲酰基肌氨酰氨基水解酶形成的水解产物（肌氨酸形成），并用肌氨酸氧化酶或肌氨酸脱氢酶检测肌氨酸，或（b）同时形成的二磷酸核苷。

4. 发明授权

US4868139A Aqueous cholesterol standard solution and process for its preparation 失效
标题翻译：水性胆固醇标准溶液及其制备方法
公开(公告)号：US4868139A
公开(公告)日：1989-09-19
申请号：US122019
申请日：1987-11-17
申请人： Rolf Deeg , Gisela Dengler , Joachim Ziegenhorn
发明人： Rolf Deeg , Gisela Dengler , Joachim Ziegenhorn
IPC分类号： G01N33/50 , C12Q1/60 , G01N33/92
CPC分类号： C12Q1/60 , Y10T436/104165 , Y10T436/108331
摘要： The present invention provides an aqueous cholesterol standard solution with a definite content of cholesterol, wherein it contains a detergent mixture of 10 to 90% of cholic acid and 90 to 10% of desoxycholic acid or of appropriate salts or derivatives of these acids.The present invention also provides a process for the preparation of this aqueous cholesterol standard solution, wherein a detergent mixture of cholic acid and desoxycholic acid or of appropriate salts and derivatives of these acids is dissolved in distilled water or in 0.9% aqueous sodium chloride solution, an appropriate preservation agent and/or a buffer effective in the pH range of from 7 to 9 optionally added thereto, and a definite, precisely defined amount of cholesterol is dissolved in the solution thus obtained, while stirring and warming to 40.degree. to 60.degree. C.
摘要翻译：本发明提供具有一定胆固醇含量的胆固醇水溶液，其中含有10-90％胆酸和90-10％脱氧胆酸或这些酸的适当盐或衍生物的洗涤剂混合物。本发明还提供了制备这种胆固醇水溶液标准溶液的方法，其中将胆酸和脱氧胆酸的洗涤剂混合物或这些酸的适当盐和衍生物溶解在蒸馏水中或在0.9％氯化钠水溶液中，适当的保存剂和/或在任意添加的pH范围为7〜9的有效缓冲液中，将一定精确定量的胆固醇溶于所得溶液中，同时搅拌并升温至40〜60℃ C。

5. 发明授权

US4503144A Method and composition for the determination of cholesterol 失效
标题翻译：测定胆固醇的方法和组成
公开(公告)号：US4503144A
公开(公告)日：1985-03-05
申请号：US328350
申请日：1981-12-07
申请人： Rolf Deeg , Helmut Schlumberger , Joachim Ziegenhorn
发明人： Rolf Deeg , Helmut Schlumberger , Joachim Ziegenhorn
IPC分类号： G01N33/50 , C12Q1/60 , C12Q1/26 , C12Q1/28 , C12Q1/44 , C12R1/465
CPC分类号： C12Q1/60 , Y10S435/805 , Y10S435/81 , Y10S435/886
摘要： The present invention provides a process for the determination of cholesterol in free or bound form by means of cholesterol oxidase and optionally of cholesterol esterase by measurement of the oxygen consumption, of the hydrogen peroxide formed or of the cholestenone formed, wherein the determination is carried out kinetically with the use of a cholesterol oxidase which has been obtained from a micro-organism of the genus Streptomyces and with the addition of 3,4-dichlorophenol.The present invention also provides a reagent for the kinetic determination of cholesterol, comprising cholesterol oxidase and a system for the determination of hydrogen peroxide or of cholestenone and optionally cholesterol esterase, wherein it additionally contains 3,4-dichlorophenol and the cholesterol oxidase used originates from a micro-organism of the genus Streptomyces.
摘要翻译：本发明提供了一种通过胆固醇氧化酶和任选的胆固醇酯酶通过测量所形成的过氧化氢或形成的胆碱酯的氧消耗来测定游离或结合形式的胆固醇的方法，其中进行测定动态地使用已经从链霉菌属的微生物获得的胆固醇氧化酶，并加入3,4-二氯苯酚。本发明还提供用于胆固醇的动力学测定的试剂，其包含胆固醇氧化酶和用于测定过氧化氢或胆石素和任选的胆固醇酯酶的系统，其中还含有3,4-二氯苯酚，所用的胆固醇氧化酶源自链霉菌属的微生物。

6. 发明授权

US4645739A Process and reagent for the determination of N-carbamoylsarcosine with the use of a new enzyme 失效
标题翻译：使用新酶测定N-氨基甲酰肌氨酸的方法和试剂
公开(公告)号：US4645739A
公开(公告)日：1987-02-24
申请号：US562072
申请日：1983-12-16
申请人： Rolf Deeg , Albert Roder , Joachim Siedel , Helmgard Gauhl , Joachim Ziegenhorn
发明人： Rolf Deeg , Albert Roder , Joachim Siedel , Helmgard Gauhl , Joachim Ziegenhorn
IPC分类号： C12N9/80 , C12Q1/34 , C12R1/01 , C12R1/06 , C12R1/265 , G01N33/70 , C12Q1/26 , C12Q1/00 , C12Q1/28
CPC分类号： G01N33/70 , C12N9/80 , C12Q1/34 , G01N2333/98 , Y10S435/81 , Y10S435/814 , Y10S435/815 , Y10S435/816 , Y10S435/822 , Y10S435/83 , Y10S435/859
摘要： The present invention provides a process for the determination of N-carbamoylsarcosine, wherein a sample solution containing N-carbamoylsarcosine is reacted with N-carbamoylsarcosine-amidohydrolase to give sarcosine, which is then determined.The present invention also provides the enzyme N-carbamoylsarcosine-amidohydrolase, a process for obtaining it and a reagent containing it.
摘要翻译：本发明提供了测定N-氨基甲酰基肌氨酸的方法，其中将含有N-氨基甲酰基肌氨酸的样品溶液与N-氨基甲酰基肌氨酸 - 酰胺水解酶反应，得到肌氨酸，然后测定。本发明还提供了N-氨基甲酰基肌氨酸 - 酰氨基水解酶，其获得方法和含有它的试剂。

7. 发明授权

US5362624A Process for the determination of an immunologically detectable substance and a suitable reaction vessel therefor 失效
标题翻译：用于测定免疫学可检测物质及其合适的反应容器的方法
公开(公告)号：US5362624A
公开(公告)日：1994-11-08
申请号：US925300
申请日：1992-08-05
申请人： Urban Schmitt , Eberhard Maurer , Wolfgang Rudinger , Rolf Deeg , Thomas Geiger , Wolf D. Engel
发明人： Urban Schmitt , Eberhard Maurer , Wolfgang Rudinger , Rolf Deeg , Thomas Geiger , Wolf D. Engel
IPC分类号： G01N33/531 , G01N33/543 , G01N33/569 , G01N33/574 , G01N33/58 , C01N33/576 , C01N33/547
CPC分类号： G01N33/57473 , G01N33/531 , G01N33/54366 , G01N33/56988 , G01N33/58 , Y10S435/82 , Y10S436/811
摘要： For the determination of an immunologically detectable substance based on a heterogeneous immunoassay by use of a solid phase on which one of the immunologically active reaction components is bound, a reaction vessel is used as the solid phase on the inner surface of which streptavidin or avidin is bound in such an amount that 0.1 to 2.5 .mu.g are present per ml reaction volume.A suitable reaction vessel for this has optically transparent wall areas which face one another and has avidin or streptavidin coated walls which are at least partially within the inner wall region intended as a receptacle for liquid, wherein the inner space of the container intended as a receptacle for liquid and the respective streptavidin or avidin content of the coating are so matched that 0.1 to 2.5 .mu.g streptavidin or avidin are present per ml reaction volume.
摘要翻译：为了通过使用其上结合有免疫活性的反应组分的固相来测定基于非均相免疫测定的免疫学可检测物质，使用反应容器作为其内表面上的固相，其中链霉亲和素或抗生物素蛋白为结合量为每ml反应体积存在0.1至2.5μg。合适的反应容器具有相互面对的光学透明壁区域，并且具有至少部分位于旨在作为液体容器的内壁区域内的抗生物素蛋白或链霉抗生物素蛋白包被的壁，其中容器的内部空间用作容器对于液体，并且涂层的各自的链霉抗生物素蛋白或抗生物素蛋白含量如此匹配，每ml反应体积存在0.1至2.5μg链霉亲和素或抗生物素蛋白。

8. 发明授权

US5776702A Process for the determination of an immunologically detectable substance and a suitable reaction vessel therefor 失效
标题翻译：用于测定免疫学可检测物质及其合适的反应容器的方法
公开(公告)号：US5776702A
公开(公告)日：1998-07-07
申请号：US271855
申请日：1994-07-07
申请人： Urban Schmitt , Eberhard Maurer , Wolfgang Rudinger , Rolf Deeg
发明人： Urban Schmitt , Eberhard Maurer , Wolfgang Rudinger , Rolf Deeg
IPC分类号： G01N33/531 , G01N33/543 , G01N33/569 , G01N33/574 , G01N33/58 , G01N33/547
CPC分类号： G01N33/57473 , G01N33/531 , G01N33/54366 , G01N33/56988 , G01N33/58 , Y10S435/82 , Y10S436/811
摘要： For the determination of an immunologically detectable substance based on a heterogeneous immunoassay by use of a solid phase on which one of the immunologically active reaction components is bound, a reaction vessel is used as the solid phase on the inner surface of which streptavidin or avidin is bound in such an amount that 0.1 to 2.5 .mu.g are present per ml reaction volume. A suitable reaction vessel for this has optically transparent wall areas which face one another and has avidin or streptavidin coated walls which are at least partially within the inner wall region intended as a receptacle for liquid, wherein the inner space of the container intended as a receptacle for liquid and the respective streptavidin or avidin content of the coating are so matched that 0.1 to 2.5 .mu.g streptavidin or avidin are present per ml reaction volume.
摘要翻译：为了通过使用其上结合有免疫活性的反应组分的固相来测定基于非均相免疫测定的免疫学可检测物质，使用反应容器作为其内表面上的固相，其中链霉亲和素或抗生物素蛋白为结合量为每ml反应体积存在0.1至2.5μg。合适的反应容器具有相互面对的光学透明壁区域，并且具有至少部分位于旨在作为液体容器的内壁区域内的抗生物素蛋白或链霉抗生物素蛋白包被的壁，其中容器的内部空间用作容器对于液体，并且涂层的各自的链霉抗生物素蛋白或抗生物素蛋白含量如此匹配，每ml反应体积存在0.1至2.5μg链霉亲和素或抗生物素蛋白。

9. 再颁专利

USRE34312E Process and reagent for the determination of an antibody 失效
公开(公告)号：USRE34312E
公开(公告)日：1993-07-13
申请号：US796545
申请日：1991-11-21
申请人： Thomas Geiger , Wolf D. Engel , Urban Schmitt , Eberhard Maurer , Wolfgang Rudinger , Rolf Deeg
发明人： Thomas Geiger , Wolf D. Engel , Urban Schmitt , Eberhard Maurer , Wolfgang Rudinger , Rolf Deeg
IPC分类号： G01N33/543 , G01N33/576 , G01N33/68
CPC分类号： G01N33/6854 , G01N33/5764 , Y10S435/805 , Y10S435/81 , Y10S436/827
摘要： The present invention provides a process for the determination of an antibody by incubation with three different reagents R.sub.1, R.sub.2 and R.sub.3, of which R.sub. and R.sub.3 are present in liquid phase and are bindable with the antibody, R.sub.2 is present bound to a solid phase and is bindable with R.sub.1 and R.sub.3 carries a label, separation of the solid phase from the liquid phase and measurement of the label in the solid phase, wherein as R.sub.1 there is used a conjugate of a substance specifically recognized by the antibody to be determined and a reaction component of a specific binding system, as R.sub.3 there is used a conjugate of a substance specifically recognized by the antibody to be determined and a label and as R.sub.2 there used the other binding component of the specific binding system. The present invention also provides a composition for the determination of an antibody, wherein it contains two different soluble reagents R.sub.1 and R.sub.3 specifically bindable with the antibody, of which R.sub.1 carries a reaction component of a specific binding system and R.sub.3 carries a label and also a receptor R.sub.2 which is present in solid phase and is bindable with the receptor R.sub.1, the insoluble receptor R.sub.2 and the soluble receptor R.sub.1 being present physically separated from one another.

10. 发明授权

US06630350B1 Monoclonal antibodies against a human act and serine protease complex 失效
标题翻译：针对人类行为和丝氨酸蛋白酶复合物的单克隆抗体
公开(公告)号：US06630350B1
公开(公告)日：2003-10-07
申请号：US09284128
申请日：1999-09-03
申请人： Christa Hubner-Parajsz , Hartmut Schetters , Rosemarie Kientsch-Engel , Thomas Meier , Martin Kaufmann , Andreas Gallusser , Rolf Deeg
发明人： Christa Hubner-Parajsz , Hartmut Schetters , Rosemarie Kientsch-Engel , Thomas Meier , Martin Kaufmann , Andreas Gallusser , Rolf Deeg
IPC分类号： C12H1585
CPC分类号： C07K14/705 , C07K16/38 , C07K16/40 , G01N33/57434
摘要： The invention concerns monoclonal antibodies against a complex of human ACT and a serine protease, preferably against a ACT-PSA, which have essentially no cross-reactivity with free, non-complexed human ACT and with free, non-complexed PSA, as well as diagnostic test methods for detecting serine protease-ACT complexes, in particular PSA-ACT, using these monoclonal antibodies.
摘要翻译：本发明涉及针对人ACT和丝氨酸蛋白酶的复合物的抗体，优选地针对ACT-PSA，其与游离的，非络合的人ACT和游离的，非络合的PSA基本上不具有交叉反应性，以及使用这些单克隆抗体检测丝氨酸蛋白酶-ACT复合物，特别是PSA-ACT的诊断测试方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式