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21. 发明专利

JPH01168256A PREPARATION OF NATURAL FLAVOR ORIGINATED FROM LACTOBACILLUS STARTER 失效
公开(公告)号：JPH01168256A
公开(公告)日：1989-07-03
申请号：JP32741387
申请日：1987-12-25
申请人： SNOW BRAND MILK PROD CO LTD
发明人： SHIYUKUNOBE YUKITAKA , TAKATO SHINICHI
IPC分类号： A23C21/02 , A23C9/12 , A23L27/24 , C12P7/26 , C12P39/00 , C12R1/46
摘要： PURPOSE:To obtain a natural flavor, by adding citric acid (salt) to animal milk or whey, inoculating and culturing two specific kinds of lactobacilli in the medium, adding citric acid (salt) to the cultured product, subjecting to steam- distillation, adding an oxidizing agent to the distillate and subjecting again to steam-distillation. CONSTITUTION:Citric acid (salt) is added to animal milk or whey. Two kinds of lactobacilli belonging to genus Streptococcus cremoris and genus Streptococcus diacetilactis are inoculated to and cultured in the above medium. The cultured produce is added with citric acid (salt) and subjected to steam-distillation. The obtained distillate is added with an oxidizing agent (e.g., ferric chloride) and subjected again to steam-distillation to obtain a natural flavor having high diacetyl content and suitable for foods such as butter.

22. 发明专利

JPH01128784A PRODUCTION OF CELL CAPABLE OF HIGHLY PRODUCING VITAMIN K1 BY TISSUE CULTIVATION OF IPOMOEA BATATAS L. 失效
公开(公告)号：JPH01128784A
公开(公告)日：1989-05-22
申请号：JP28702287
申请日：1987-11-13
申请人： SNOW BRAND MILK PROD CO LTD
发明人： FUJITA TAKASHI , HATAMOTO HITOSHI , TAKATO SHINICHI
IPC分类号： C12N5/04 , C12N5/00 , C12P7/66 , C12R1/91
摘要： PURPOSE:To obtain cells capable of highly producing vitamin K1, by inducing calluses from a root tuber of Ipomoea batatas L. and subculturing the above- mentioned calluses which are being irradiated with light. CONSTITUTION:A root tuber of Ipomoea batatas L. is cut to a suitable size to provide tissual pieces, which are then cultivated to induce calluses. The resultant calluses are preferably subcultured in the presence of plant hormones, such as auxins and cytokinins, while being irradiated with light to provide cells capable of highly producing vitamin K1. Alternatively, adventitious roots differentiated by cultivating the calluses are also cultivated while being irradiated with light to afford the aimed cells capable of highly producing vitamin K1.

23. 发明专利

JPS6219812B2 失效
公开(公告)号：JPS6219812B2
公开(公告)日：1987-05-01
申请号：JP14489479
申请日：1979-11-08
申请人： SNOW BRAND MILK PROD CO LTD
发明人： TAKATO SHINICHI , KITAMURA KATSUSHI , YOSHIOKA YASUO
IPC分类号： A23C19/00 , A23C19/068 , A23C20/00

24. 发明专利

JPS61254185A METHOD OF CONTINUOUS CULTURE IN HIGH CONCENTRATION AND DEVICE THEREFOR 失效
公开(公告)号：JPS61254185A
公开(公告)日：1986-11-11
申请号：JP9416285
申请日：1985-05-01
申请人： SNOW BRAND MILK PROD CO LTD
发明人： KUMAZAWA EITARO , SATOU MASAO , MINAMIDE YOSHITOMO , TAKATO SHINICHI
IPC分类号： C12M1/12 , C12M1/00 , C12N1/00
摘要： PURPOSE:To eliminate loss of a medium fed and to raise its use efficiency, sending a mold solution remaining at a medium feed side to a filtered solution discharge side by the use of an inert gas when plural filter members are changed. CONSTITUTION:The solenoid value IF is closed, the solenoid valves 2F-4F and the solenoid valves 2P-4P at the filtered solution side are opened, a mold solution in the culture tank 10 is sent through the pump 38, the pipes 39 and 42-44 and the filter chambers MF2-4, filtered, and the filtered solution discharged from the lines 57-59 and 55. On the other hand, the mold solution is circulated from the filter chamber MF1 and the line 50 to the tank 10. Then, an inert gas from the tank 76 is fed under pressure from the top of the membrane of the chamber MF2 through the solenoid valves 2G and 2H to the chamber, a filtered solution in a membrane cartridge is sent through the valve 2P and discharged from the pipe 55. Simultaneously channels are changed, the inert gas is fed under pressure through the solenoid valves 1G and 1H to the chamber MF1, and a medium in the chamber MF1 if transferred through the solenoid valves 1S and 2S to the chamber MF2.

25. 发明专利

JPS61254184A METHOD OF CONTINUOUS CULTURE IN HIGH CONCENTRATION AND DEVICE THEREFOR 失效
公开(公告)号：JPS61254184A
公开(公告)日：1986-11-11
申请号：JP9416185
申请日：1985-05-01
申请人： SNOW BRAND MILK PROD CO LTD
发明人： KUMAZAWA EITARO , SATOU MASAO , MINAMIDE YOSHITOMO , TAKATO SHINICHI
IPC分类号： C12M1/12 , C12M1/00 , C12N1/00
摘要： PURPOSE:To utilize effectively a membrane area and to cultivate continuously a mold in high concentration, by discharging a filtered solution from some filter membranes among plural filter membranes, and changing channels at fixed intervals. CONSTITUTION:The solenoid value 1F is closed, the solenoid valves 2F-4F are opened, the solenoid valves 2P-4P at the filtered solution side are opened, a mold solution in the culture tank 10 is passed through the pipe 66, the circulating pump 38 and the pipes 42-44, sent to the filter changers MF2-4, filtered, the filtered solution is sent through the pipes 57-59 and the pipe 55 and discharged. The mold solution is separated into the filter chamber MF1 and the pipe 49, blended with a medium sent through the solenoid value 1S, provided with a reverse washing effect and circulated through the culture tank 10 (Step 1). Then, after a fixed time is over, the mold solution is circulated while being provided with reverse washing effect by the operation of the solenoid valves shown in the Steps 2-3. through the filter chambers MF2-4.

26. 发明专利

JPS6188872A Method and apparatus for continuous cultivation at high concentration 失效
标题翻译：在高浓度下连续培养的方法和装置
公开(公告)号：JPS6188872A
公开(公告)日：1986-05-07
申请号：JP21174784
申请日：1984-10-09
申请人： Snow Brand Milk Prod Co Ltd
发明人： KUMAZAWA EITARO , SATOU KISAO , MINAMIDE YOSHITOMO , TAKATO SHINICHI , IMON KAZUO
IPC分类号： C12M1/12 , C12M1/00 , C12M1/40 , C12N1/00
摘要： PURPOSE: To enable the culture of microbial cells in high concentration, by supplying the culture medium to the second filtration membrane in the case of normal flow direction in the circulation circuit, and supplying to the first filtration membrane in the case of reverse flow and continuing the cultivation while removing the metabolic products from the medium.
CONSTITUTION: A seed microorganism is inoculated in the culture tank 10, the cultivation is started, and when the concentration of the metabolic product reaches a specific level, the operation of membranes is started. In the case of the normal flow operation shown by the solid arrow, the cell liquid extracted from the culture tank 10 with the pump 37 is passed in the order of the first and the second filtration chambers 11, 12. The first filtration chamber 11 is operated under higher pressure, and the filtrate containing the metabolic product is introduced into the filtration chamber 27, the metabolic product is removed by the RO membrane, and the nutrient component having low molecular weight is returned to the medium supply tank 29. When the system is turned to the reverse-flow operation shown by the dotted arrow by the simultaneous change-over of the three-way solenoid valves 19, 20, 21, 23 and 32, the first filtration chamber is subjected to the back-washing, and is supplied with low-molecular nutrient component.
COPYRIGHT: (C)1986,JPO&Japio
摘要翻译：目的：为了能够高浓度培养微生物细胞，通过在循环回路中在正常流动方向的情况下向第二过滤膜供应培养基，并且在反向流动的情况下供给第一过滤膜并继续培养同时从培养基中除去代谢产物。构成：将种子微生物接种在培养槽10中，开始培养，当代谢产物的浓度达到特定水平时，开始膜的运转。在通过实线箭头所示的正常流动操作的情况下，用泵37从培养罐10中提取的细胞液体以第一和第二过滤室11,12的顺序通过。第一过滤室11是在较高压力下操作，将含有代谢产物的滤液引入过滤室27中，通过RO膜除去代谢产物，并且具有低分子量的营养成分返回到介质供应罐29.当系统通过三通电磁阀19,20,21,23和32的同时转换转到虚箭头所示的反向流动操作，第一过滤室进行反洗，并且提供低分子营养成分。

27. 发明专利

JPS597414B2 JPS597414B2 - 失效
公开(公告)号：JPS597414B2
公开(公告)日：1984-02-18
申请号：JP15978580
申请日：1980-11-13
申请人： Snow Brand Milk Prod Co Ltd
发明人： IZUTSU MASA , KAMITAKE MASANOBU , HIRAOKA YASUNOBU , TAKATO SHINICHI , YAMADA TAMOTSU , KUROSAWA SEIJI , SATO TETSUO
IPC分类号： A23G3/20 , A01J25/00 , A01J25/12 , A23J3/00 , A23J3/10
CPC分类号： A01J25/008 , A01J25/12 , Y10S426/802

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