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21. 发明申请

WO2007007933A1 METHOD OF IN-SILICO IMPROVEMENT OF ORGANISMS USING THE FLUX SUM OF METABOLITES 审中-公开
标题翻译：使用代谢物通量的有机硅改善方法
公开(公告)号：WO2007007933A1
公开(公告)日：2007-01-18
申请号：PCT/KR2005/003442
申请日：2005-10-14
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , KIM, Tae Yong , LEE, Dong Yup
发明人： LEE, Sang Yup , KIM, Tae Yong , LEE, Dong Yup
IPC分类号： C12Q1/68
CPC分类号： G06F19/12 , G06F19/18
摘要： The present invention relates to an in silico method for improving an organism on the basis of the flux sum (Φ) of metabolites, and more particularly to a method for screening key metabolites that increase the production yield of a useful substance, the method comprising defining the metabolite utilization of an organism for producing a useful substance as flux sum and perturbing the flux sum, as well as a method for improving an organism producing a useful substance, the method comprising deleting and/or amplifying genes associated with the aforementioned screened key metabolites. According to the present invention, the correlation between specific metabolites and useful substance production can be exactly predicted, so that it is possible to develop an organism having increased useful substance production by introducing and/or amplifying and/or deleting genes expressing enzymes associated with the specific metabolites. In addition, it is also possible to increase the production of a useful substance by adding specific metabolites during culture.
摘要翻译：本发明涉及一种用于基于代谢物的通量和（F）改善生物体的计算机方法，更具体地涉及一种用于筛选提高有用物质的产量的关键代谢物的方法，所述方法包括定义用于产生有用物质的生物体的代谢物利用作为助熔剂和扰乱通量和，以及改善产生有用物质的生物体的方法，所述方法包括删除和/或扩增与上述筛选的关键代谢物相关的基因。根据本发明，可以准确地预测特定代谢物和有用物质生产之间的相关性，从而可以通过引入和/或扩增和/或删除表达与...相关的酶的基因来开发具有增加的有用物质生产的生物体特异性代谢物。此外，还可以通过在培养期间加入特定的代谢物来增加有用物质的产生。

22. 发明申请

WO2005052135A1 NOVEL RUMEN BACTERIA VARIANTS AND PROCESS FOR PREPARING SUCCINIC ACID EMPLOYING THE SAME 审中-公开
标题翻译：新型葡萄酒细菌变异体及其制备使用同系酸的方法
公开(公告)号：WO2005052135A1
公开(公告)日：2005-06-09
申请号：PCT/KR2004/001210
申请日：2004-05-20
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , LEE, Sang Jun
发明人： LEE, Sang Yup , LEE, Sang Jun
IPC分类号： C12N1/21
CPC分类号： C12P7/46 , C12N1/20 , C12R1/01
摘要： The present invention relates to novel rumen bacterial mutants resulted from the disruption of a lactate dehydrogenase gene ( ldhA ) and a pyruvate formate-lyase gene ( pf l) (which are involved in the production of lactic acid, formic acid and acetic acid) from rumen bacteria; a novel bacterial mutant ( Mannheimia sp. LPK7) having disruptions of a lactate dehydrogenase gene ( ldhA ), a pyruvate formate-lyase gene ( plf ), a phosphotransacetylase gene ( pta ), and a acetate kinase gene ( ackA ); a novel bacterial mutant ( Mannheimia sp. LPK4) having disruptions of a lactate dehydrogenase gene ( ldhA ), a pyruvate formate-lyase gene ( pfl ) and a phosphoenolpyruvate carboxylase gene ( ppc ) involved in the immobilization of CO2 in a metabolic pathway of producing succinic acid; and a method for producing succinic acid, which is characterized by the culture of the above mutants in anaerobic conditions. The inventive bacterial mutants have the property of producing succinic acid at high concentration while producing little or no organic acids, as compared to the prior wild-type strains of producing various organic acids. Thus, the inventive bacterial mutants are useful as strains for the industrial production of succinic acid.
摘要翻译：本发明涉及由瘤胃中的乳酸脱氢酶基因（ldhA）和丙酮酸甲酸裂解酶基因（pfl）（其涉及乳酸，甲酸和乙酸的生产）的破坏而产生的新型瘤胃细菌突变体菌; 具有乳酸脱氢酶基因（ldhA），丙酮酸甲酸裂合酶基因（plf），磷酸转乙酰酶基因（pta）和乙酸激酶基因（ackA）的破坏的新型细菌突变体（曼氏血清杆菌LPK7）; 具有破坏乳酸脱氢酶基因（ldhA），丙酮酸甲酸裂合酶基因（pfl）和磷酸烯醇丙酮酸羧化酶基因（ppc）的新型细菌突变体（曼氏血吸虫属LPK4），其涉及将CO 2固定在生产代谢途径中琥珀酸; 以及生产琥珀酸的方法，其特征在于在厌氧条件下培养上述突变体。与生产各种有机酸的现有野生型菌株相比，本发明的细菌突变体具有以高浓度生产琥珀酸的同时产生很少或不含有机酸的性质。因此，本发明的细菌突变体可用作工业生产琥珀酸的菌株。

23. 发明申请

WO2005023847A1 COMPOSITION FOR PROTECTING PROTEINS DEGRADATION COMPRISING SMALL HEAT SHOCK PROTEINS (sHSPs) AND METHOD OF TWO-DIMENSIONAL GEL ELECTROPHORESIS USING THE sHSPs 审中-公开
标题翻译：用于保护包含小热休克蛋白（sHSP）的蛋白质降解的组合物和使用sHSP的两维凝胶电泳方法
公开(公告)号：WO2005023847A1
公开(公告)日：2005-03-17
申请号：PCT/KR2003/002539
申请日：2003-11-24
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , HAN, Mee Jung , PARK, Si Jae
发明人： LEE, Sang Yup , HAN, Mee Jung , PARK, Si Jae
IPC分类号： C07K14/00
CPC分类号： C07K14/195 , C07K14/00
摘要： The present invention relates to a composition containing sHSPs for prevention of protein degradation and a composition for two-dimensional gel electrophoresis. Furthermore, the present invention relates to the improved method of two-dimensional gel electrophoresis, which is characterized by using sHSPs. According to the present invention, decreasing of protein spots was prevented in the two-dimensional gel electrophoresis, thereby obtaining two-dimensional gel with much more protein spots.
摘要翻译：本发明涉及含有用于预防蛋白质降解的sHSP和用于二维凝胶电泳的组合物的组合物。此外，本发明涉及二维凝胶电泳的改进方法，其特征在于使用sHSP。根据本发明，在二维凝胶电泳中可以防止蛋白质斑点的减少，从而获得蛋白质斑点多的二维凝胶。

24. 发明申请

WO2009082148A2 ENHANCED ETHANOL AND BUTANOL PRODUCING MICROORGANISMS AND METHOD FOR PREPARING ETHANOL AND BUTANOL USING THE SAME 审中-公开
标题翻译：生产微生物的增强的乙醇和丁醇和使用它们制备乙醇和丁醇的方法
公开(公告)号：WO2009082148A2
公开(公告)日：2009-07-02
申请号：PCT/KR2008/007577
申请日：2008-12-22
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , BIOFUELCHEM CO. , GS CALTEX CORPORATION , LEE, Sang Yup , JANG, Yu-Sin , LEE, Jin Young , JUNG, Kwang Seop , KIM, Jae Hyun
发明人： LEE, Sang Yup , JANG, Yu-Sin , LEE, Jin Young , JUNG, Kwang Seop , KIM, Jae Hyun
IPC分类号： C12N15/09
CPC分类号： C12P7/065 , C12N9/0006 , C12N9/13 , C12P7/16 , Y02E50/10 , Y02E50/17
摘要： The present invention relates to a recombinant microorganism having an enhanced ability to produce ethanol and butanol and a method for preparing ethanol and butanol using the same, and more particularly to a recombinant microorganism having an enhanced ability to produce ethanol and butanol, into which a gene encoding CoA transferase and a gene encoding alcohol/aldehyde dehydrogenase are introduced, and to a method for preparing ethanol and butanol using the same. The recombinant microorganism according to the present invention, obtained by manipulating metabolic pathways of microorganisms, is capable of producing butanol and ethanol exclusively without producing any byproduct, and thus is useful as a microorganism producing industrial solvents and transportation fuel.
摘要翻译：本发明涉及具有提高乙醇和丁醇生产能力的重组微生物，以及使用该重组微生物制备乙醇和丁醇的方法，更具体地说，涉及生产乙醇和丁醇的能力增强的重组微生物，其中基因引入编码CoA转移酶和编码醇/醛脱氢酶的基因，以及使用其制备乙醇和丁醇的方法。根据本发明的通过操纵微生物代谢途径获得的重组微生物能够仅生产丁醇和乙醇而不产生任何副产物，因此可用作产生工业溶剂和运输燃料的微生物。

25. 发明申请

WO2009031762A2 RECOMBINANT MICROORGANISM CAPABLE OF PRODUCING POLYLACTATE OR POLYLACTATE COPOLYMER FROM SUCROSE AND METHOD FOR PRODUCING POLYLACTATE OR POLYLACTATE COPOLYMER FROM SUCROSE USING THE SAME 审中-公开
标题翻译：由SUCROSE生产聚合物或聚氨酯共聚物的重组微生物和使用其制备聚酯或聚氨酯共聚物的方法
公开(公告)号：WO2009031762A2
公开(公告)日：2009-03-12
申请号：PCT/KR2008/004611
申请日：2008-08-08
申请人： LG CHEM, LTD. , KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , PARK, Si Jae , KIM, Tae Wan , KANG, Hye Ok , YANG, Taek Ho , LEE, Sang Yup
发明人： PARK, Si Jae , KIM, Tae Wan , KANG, Hye Ok , YANG, Taek Ho , LEE, Sang Yup
IPC分类号： C12N15/05
CPC分类号： C12N15/8243 , C12N9/1025 , C12N15/8245 , C12P7/625
摘要： Provided are a microorganism capable of producing a polylactate (PLA) or PLA copolymer from sucrose and a method of producing the PLA or PLA copolymer from sucrose using the same. A gene of an enzyme converting lactate into lactyl-CoA and a gene of a polyhydroxyalkanoate (PHA) synthase using lactyl-CoA as a substrate are introduced into a microorganism capable of using sucrose as a substrate, and the microorganism is cultured using sucrose as the substrate, thereby allowing efficient production of a PLA or PLA copolymer.
摘要翻译：提供能够从蔗糖生产聚乳酸（PLA）或PLA共聚物的微生物和使用其从蔗糖生产PLA或PLA共聚物的方法。将使用乳酰基辅酶A作为底物的将乳酸酯转化为乳酰基-CoA的酶基因和聚羟基链烷酸酯（PHA）合成酶的基因导入到能够使用蔗糖作为底物的微生物中，并使用蔗糖作为培养基从而能够有效地生产PLA或PLA共聚物。

26. 发明申请

WO2007078127A1 METHOD FOR CELL SURFACE DISPLAYING OF TARGET PROTEINS USING BACILLUS ANTHRACIS EXOSPORIUM 审中-公开
标题翻译：用BACILLUS ANTHRACIS EXOSPORIUM对目标蛋白进行细胞表面展示的方法
公开(公告)号：WO2007078127A1
公开(公告)日：2007-07-12
申请号：PCT/KR2006/005886
申请日：2006-12-29
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , PARK, Tae Jung , HEO, Nam Su
发明人： LEE, Sang Yup , PARK, Tae Jung , HEO, Nam Su
IPC分类号： C12N15/64 , C12N15/70 , C12N15/63
CPC分类号： C07K14/32 , C07K16/00 , C07K2317/622 , C07K2319/00 , C12N15/70 , C12P21/02
摘要： The present invention relates to a method for expressing a target protein on the surface of a microorganism using Bacillus anthracis exosporium protein. More particularly, to an expression vector constructed such that it comprises bclA gene encoding Bacillus anthracis exosporium protein BcIA or fragements thereof as a cell surface anchoring motif and the target protein can be expressed on the surface of a cell in a form fused with BcIA or a fragment thereof when the gene encoding the target protein is expressed in a host cell, as well as, a method for expressing a target protein on the surface of a microorganism using the vector. The expression vector according to the present invention is capable of effectively expressing a target protein or a peptide on the cell surface using BcIA, Bacillus anthracis exosporium protein as a cell surface anchoring motif, and since a target protein can be stably expressed on the cell surface in large amounts by culturing a microorganism transformed with the expression vector, thus making it possible to effectively use for the various purposes of recombinant live vaccines, whole cells absorbents, whole cell bioconversion and the like.
摘要翻译：本发明涉及使用炭疽芽孢杆菌外孢菌蛋白质在微生物表面上表达靶蛋白质的方法。更具体地说，涉及这样构建的表达载体，即它包含编码炭疽芽孢杆菌外孢菌蛋白BcA1的bclA基因或其片段作为细胞表面锚定基序，并且靶蛋白可以在细胞表面表达当编码靶蛋白质的基因在宿主细胞中表达时与BcA1或其片段融合的形式，以及使用该载体在微生物表面表达靶蛋白质的方法。根据本发明的表达载体能够使用BcA1，炭疽芽孢杆菌外孢菌蛋白质作为细胞表面锚定基序在细胞表面上有效表达目标蛋白质或肽，并且由于靶蛋白质可以在细胞表面上稳定表达通过培养用表达载体转化的微生物大量培养，从而有可能有效地用于重组活疫苗，全细胞吸收剂，全细胞生物转化等的各种目的。

27. 发明申请

WO2005028654A1 METHOD FOR WHOLE SURROUNDING SURFACE DISPLAY OF TARGET PROTEINS USING BACTERIAL EXOSPORIUM 审中-公开
标题翻译：使用细菌外植体全面表面显示目标蛋白的方法
公开(公告)号：WO2005028654A1
公开(公告)日：2005-03-31
申请号：PCT/KR2003/002882
申请日：2003-12-30
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , PARK, Tae Jung , PARK, Jong Pil , LEE, Seok Jae
发明人： LEE, Sang Yup , PARK, Tae Jung , PARK, Jong Pil , LEE, Seok Jae
IPC分类号： C12N15/63
CPC分类号： C40B40/02 , C12N15/1037
摘要： The present invention relates to a method for expressing a target protein on an exosporium forming the outermost surface of bacterial spores. More particularly, the present invention relates to a method for expressing a target protein on the surface of cells and spores using an exosporium as a matrix for surface expression, and methods for the production of a protein array, the production of antibodies, the separation of a certain substance from a mixture, bioconversion, and the improvement of a target protein, which are characterized by using the cells or spores having the target protein that was expressed on the surface by the above expression method. The method for expressing the target protein on the surface of the spore outer membrane of the gene carriers according to the present invention has effects in that a variety of the target proteins can be expressed and the level of surface expression of the target protein is increased compared to the existing technology, and also the structural stability of the gene carriers having the target protein expressed on their surface, the viability of the host, and the rapidity of the screening method, are greatly increased.
摘要翻译：本发明涉及在形成细菌孢子的最外表面的外孢上表达靶蛋白的方法。更具体地说，本发明涉及使用外鎓作为表面表达基质在细胞和孢子表面上表达靶蛋白的方法，以及用于产生蛋白质阵列，产生抗体的方法，分离特定于使用具有通过上述表达方法在表面表达的靶蛋白的细胞或孢子的来自混合物的某种物质，生物转化和靶蛋白的改良。根据本发明的用于在基因载体的孢子外膜的表面上表达靶蛋白的方法具有以下效果：可以表达多种靶蛋白，并且与目标蛋白的表面表达水平相比提高了靶蛋白的表达表达水平现有技术，以及表面上具有靶蛋白的基因载体的结构稳定性，宿主的活力以及筛选方法的快速性都大大提高。

28. 发明公开

EP4001406A2 RECOMBINANT MICROORGANISM HAVING INCREASED ABILITY TO PRODUCE HYDROPHOBIC MATERIAL AND CELL-MEMBRANE ENGINEERING METHOD FOR PREPARATION THEREOF 无效
公开(公告)号：EP4001406A2
公开(公告)日：2022-05-25
申请号：EP21205995.0
申请日：2021-11-02
申请人： Korea Advanced Institute of Science and Technology
发明人： LEE, Sang Yup , YANG, Dongsoo , PARK, Seon Young
IPC分类号： C12N9/10 , C12P23/00 , C12P17/16 , C07K14/705 , C12N9/04 , C12N9/90 , C12N1/20 , C12N15/52
摘要： Disclosed are a recombinant microorganism for producing a hydrophobic material, which is subjected to cell-membrane engineering in order to be imparted with at least one characteristic among an increase in a cell-membrane area, an increase in formation and secretion of an outer membrane vesicle, and an increase in formation of an inner membrane vesicle, and a cell-membrane engineering method for preparation thereof, whereby an insoluble hydrophobic material can be produced with high efficiency, the recombinant microorganism for high-efficiency production of carotenoids or violacein analogues is useful for producing natural pigments, antioxidants, antibiotics, cosmetic additives, anticancer agents, food additives, or nutritional supplements, and the natural pigment production technology developed herein achieves a great increase in production ability. Therefore, the present invention is effective at preparing a recombinant strain for efficient production of a variety of industrially and medically useful metabolites and at establishing an efficient preparation method.

29. 发明公开

EP3884951A1 COMPOSITION FOR TREATING HAIR LOSS OR PROMOTING HAIR GROWTH 有权转让
公开(公告)号：EP3884951A1
公开(公告)日：2021-09-29
申请号：EP19886811.9
申请日：2019-11-21
申请人： Core Innovations Limited , Korea Advanced Institute of Science and Technology
发明人： LEE, Sang Yup , YOO, Won Min , KIM, Sooncheol , CHUNG, Hannah , NA, Deok Hyun
IPC分类号： A61K35/16 , A61K38/17 , A61P17/14 , A61K8/98 , A61K8/64 , A61Q7/00
摘要： The present invention relates to a composition for treating hair loss or promoting hair growth which contains a silk protein, and plasma or serum components. According to the present invention, due to the synergistic effect of a silk protein and plasma or serum components, the effect of promoting hair growth and the effect of treating hair loss can be expected to be more efficient.

30. 发明公开

EP3741860A1 VARIANT MICROORGANISM HAVING ABILITY TO PRODUCE 1,3-PROPANEDIOL, AND METHOD FOR PREPARING 1,3-PDO BY USING SAME 审中-公开
公开(公告)号：EP3741860A1
公开(公告)日：2020-11-25
申请号：EP19740842.0
申请日：2019-01-15
申请人： Korea Advanced Institute Of Science And Technology , Hanwha Solutions Corporation
发明人： LEE, Sang Yup , CHO, Jae Sung , KIM, Je Woong , KO, Yoo Sung , PRABOWO, Cindy Pricilia Surya , HAN, Taehee , KIM, Euiduk , CHOI, Jae Won , CHO, Changhee , LEE, Jun Kyu
IPC分类号： C12N15/77 , C12P7/18 , C07K14/245 , C12N9/04 , C12N9/12 , C12N9/88
摘要： The present disclosure relates to a mutant microorganism in which a glycerol catabolic pathway and a 1,3-PDO biosynthetic pathway are introduced into a microorganism incapable of using glycerol as a carbon source, and a method of producing 1,3-PDO using the same. According to the present disclosure, it is possible to produce 1,3-PDO while growing a mutant microorganism having 1,3-PDO production ability by using the inexpensive raw material glycerol as a single carbon source. Thus, the present disclosure is useful for the economical production of 1,3-PDO.

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