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    • 21. 发明授权
    • Antigen for developing neutralizing antibodies to human immunodeficiency virus
    • 用于发展针对人类免疫缺陷病毒的中和抗体的抗原
    • US07179468B1
    • 2007-02-20
    • US09877606
    • 2001-06-08
    • Min LuHong Ji
    • Min LuHong Ji
    • A61K39/21
    • A61K39/21A61K39/12A61K2039/545A61K2039/55566C07K14/005C07K2319/73C12N2740/16122C12N2740/16134
    • The present invention provides polypeptides comprising a stabilized trimer of the carboxyl-terminal core region of the ectodomain of an enveloped virus, such as HIV. A subject polypeptide comprises three monomers that form a trimeric coiled coil in a prefusogenic conformation of an enveloped virus such as HIV. The subject polypeptides are useful as vacciness against enveloped virus, such as HIV, which vaccines are also provided. The present invention also provides methods of vaccinating an individual to prevent or treat HIV infection or prevent or treat infection by another enveloped virus, using a subject vaccine. Antibodies or binding portions thereof raised against a C-terminus trimeric coiled coil motif of an HIV gp41 ectodomain, or carboxyl-terminal core region of the ectodomain of another enveloped virus, are also provided as are methods of making such antibodies. Methods for detecting HIV or other enveloped viruses in a sample, as well as methods of screening for drugs which can inhibit HIV infection or other enveloped virus infection, are also provided by the present invention.
    • 本发明提供了包含例如HIV的包膜病毒的胞外域的羧基末端核心区的稳定化三聚体的多肽。 主题多肽包含在包膜病毒如HIV的前引发构象中形成三聚螺旋卷曲的三种单体。 本发明的多肽可用作针对包膜病毒(例如HIV)的疫苗,其还提供疫苗。 本发明还提供使用本发明的疫苗接种个体以预防或治疗HIV感染或预防或治疗由另一包膜病毒感染的方法。 作为制备这种抗体的方法,还提供抗HIV gp41胞外域的C末端三聚体卷曲基序或另一包膜病毒的胞外域的羧基末端核心区域的抗体或其结合部分。 检测样品中HIV或其他包膜病毒的方法以及筛选可抑制HIV感染或其他包膜病毒感染的药物的方法也由本发明提供。