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    • 22. 发明专利
    • MEASUREMENT OF CELL CONCENTRATION
    • JPS56109596A
    • 1981-08-31
    • JP1005380
    • 1980-02-01
    • HITACHI LTD
    • SHIMIZU NORIOYAMAGUCHI TETSUOSAITOU SETSUOUENO MASAOOTAHARA YOUJI
    • C12Q1/06
    • PURPOSE:In the cultivation of a microorganism, the amount of nitrogen consumed by the microorganism is determined and the result and the nitrogen content of the cells that has been previously determined are used to calculate the amount of the cells in the culture medium, whereby the accurate measurement of the cell concentration is given promptly even when the cells are cultured in high concentration. CONSTITUTION:Even when the microorganism cells cultured in tank 1 reach a high concentration level, analyzer 11 determines the nitrogen content on the supernatant collected from sample tube 3 and arithmetic unit 12 calculates the amount of the cells in the tank on the basis of the data on the nitrogen content. Corresponding to the signals from the arithmetic units 12 and 14, controller 13 controls the rotation of pump 7 to keep the addition of the substrate from substrate tank 9 to the cultivation tank 1 to an appropriate level. Thus, effective cultivation of high-concentration cells can be done. Low-concentration cultivation also is effected in the same way.