专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

21. 发明公开

EP1896617A4 MULTIPLEXED AMPLIFICATION OF SHORT NUCLEIC ACIDS 有权转让
标题翻译： MULTIPLEXVERSTÄRKUNGKURZERNUKLEINSÄUREN
公开(公告)号：EP1896617A4
公开(公告)日：2009-01-21
申请号：EP06850497
申请日：2006-05-31
申请人： APPLERA CORP
发明人： LAO KAI QIN , LIVAK KENNETH J , STRAUS NEIL A
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12N15/1065 , C12Q1/6851 , C12Q1/686 , C12Q2525/301 , C12Q2525/161 , C12Q2563/179 , C12Q2537/143 , C12Q2525/207
摘要： The present teachings provide methods, compositions, and kits for reverse transcribing and amplifying small nucleic acids such as micro RNAs. High levels of multiplexing are provided by the use of a zip-coded stem-loop reverse transcription primer, along with a PCR- based pre-amplification reaction that comprises a zip-coded forward primer. Detector probes in downstream decoding PCRs can take advantage of the zip-code introduced by the stem-loop reverse transcription primer. In some embodiments, further amplification is achieved by cycling the reverse transcription reaction. The present teachings also provide compositions and kits useful for performing the reverse transcription and amplification reactions described herein.
摘要翻译：本教导提供用于反转录和扩增小核酸如微RNA的方法，组合物和试剂盒。通过使用zip编码茎环反转录引物以及包含zip编码正向引物的基于PCR的预扩增反应提供高水平的多重化。下游解码PCR中的探测器探针可以利用由茎环反转录引物引入的zip代码。在一些实施方案中，通过循环逆转录反应来实现进一步的扩增。本教导还提供可用于进行本文所述的逆转录和扩增反应的组合物和试剂盒。

22. 发明公开

EP1552001A4 METHODS AND COMPOSITIONS FOR DETECTING TARGETS 审中-公开
标题翻译：方法和组合物目标检测
公开(公告)号：EP1552001A4
公开(公告)日：2006-01-04
申请号：EP03759406
申请日：2003-09-19
申请人： APPLERA CORP
发明人： ANDERSEN MARK R , HUNKAPILLER MICHAEL W , LIVAK KENNETH J , SPIER EUGENE G , WENZ MICHAEL H
IPC分类号： C07H21/00 , C07H21/02 , C07H21/04 , C12P19/34 , C12Q20060101 , C12Q1/68
CPC分类号： C12Q1/6827 , C12Q2561/101 , C12Q2531/137 , C12Q2525/161
摘要： The present invention relates to methods and kits for detecting the presence or absence of (or quantitating) target nucleic acid sequences using ligation and amplification. The invention also relates to methods, reagents, and kits that employ addressable portions and labeled probes.

23. 发明公开

EP1468111A4 METHOD FOR OPERATING A COMPUTER AND/OR COMPUTER NETWORK TO DISTRIBUTE BIOTECHNOLOGY PRODUCTS 审中-公开
标题翻译：一种用于运行计算机和/或计算机网络的配电生物技术产品
公开(公告)号：EP1468111A4
公开(公告)日：2005-05-25
申请号：EP02784321
申请日：2002-10-30
申请人： APPLERA CORP
发明人： KOEHLER RYAN T , LIVAK KENNETH J , STEVENS JUNKO , DE LA VEGA FRANCISCO M , RHODES MICHAEL , BELLON LAURENT R
IPC分类号： G01N27/62 , C12N15/09 , C12Q1/68 , G06F17/00 , G06F19/00 , G06Q30/00
CPC分类号： C12Q1/686
摘要： A method for distributing a product to a consumer includes utilizing a computer network to interact with the consumer to obtain information that includes at least one target nucleic acid sequence; providing a forward primer sequence, a reverse primer sequence and a probe sequence having specified characteristics, wherein the forward primer sequence and the reverse primer sequence together define an amplicon sequence, the amplicon lies within the target nucleic acid sequence, and the probe sequence is complementary to a portion of the amplicon sequence; manufacturing at least one assay that includes a forward primer in accordance with the forward primer sequence, a reverse primer in accordance with the reverse primer sequence, and a probe in accordance with the probe sequence; validating one or more of the forward primer, the reverse primer, and the probe; and delivering the manufactured assay to the consumer.

24. 发明专利

AT348191T 未知
公开(公告)号：AT348191T
公开(公告)日：2007-01-15
申请号：AT01950861
申请日：2001-07-03
申请人： APPLERA CORP
发明人： BI WANLI , LIVAK KENNETH J , BLOCH WILL
IPC分类号： G01N27/62 , C12N15/09 , C12Q1/68 , C12Q1/6862 , G01N21/64 , G01N21/76 , G01N27/447 , G01N33/53 , G01N33/566 , G01N33/68

25. 发明专利

AT296899T 未知
公开(公告)号：AT296899T
公开(公告)日：2005-06-15
申请号：AT01920751
申请日：2001-03-26
申请人： APPLERA CORP
发明人： LIVAK KENNETH J , LUCERO MICHAEL Y , SHARAF MUHAMMAD A
IPC分类号： C12N15/09 , C12Q1/68 , C12Q1/6823
摘要： The invention relates to improved methods of detecting and characterizing polynucleotide sequences and variations in polynucleotide sequences. The invention further relates to a device that can be used to detect and characterize polynucleotide sequences and variations in polynucleotide sequences.

26. 发明专利

AT288964T 未知
公开(公告)号：AT288964T
公开(公告)日：2005-02-15
申请号：AT01964412
申请日：2001-08-23
申请人： APPLERA CORP
发明人： HEID CHRISTIAN , LIVAK KENNETH J
IPC分类号： G01N33/53 , C07H21/00 , C07H21/02 , C07H21/04 , C12N15/09 , C12P19/34 , C12Q1/68 , C12Q1/70
摘要： External control reagents for nucleic acid amplification are provided that verify the absence or presence of specific target sequences, and correct primers and probes. A single-stranded, external control polynucleotide is amplified with primers of the same sequence as target primers. Probes with detectable labels and sequences specific for target and external control polynucleotides allow for detection and measurement. The primers and the detectable probe are adjacent or substantially adjacent when hybridized to the external control polynucleotide. Target and control amplicons may be detected by increased fluorescence induced by polymerase-mediated 5' nuclease cleavage or hybridization of a self-quenching probe complementary to both target and external control polynucleotides. A kit of PCR reagents can be dispensed into vessels for rapid and accurate nucleic acid amplification assay, with real-time or end-point measurements. The amplification control reagents, kits, and methods of the present invention provide positive and negative control tests which can be conducted concurrently with target amplification. Allelic differences at genetic loci can be detected, including single nucleotide polymorphisms (SNP).

27. 发明专利

CA2474482A1 METHODS FOR PLACING, ACCEPTING, AND FILLING ORDERS FOR PRODUCTS AND SERVICES 未知
公开(公告)号：CA2474482A1
公开(公告)日：2003-08-07
申请号：CA2474482
申请日：2003-01-02
申请人： APPLERA CORP
发明人： GILBERT DENNIS A , MADDEN DAWN , XU LILY , SCOTT JOHN , HEMKEN HEINZ , AVI-ITZHAK HADAR I , SCAFE CHARLES R , TITUS ANNIE , CURLEE JOANNA , YOU XIAOQING , WINN-DEEN EMILY , GLANOWSKI STEPHEN , WU LIN , HEIL JEREMY , MCCULLEN IVY , GIRE CARRY , WILLIAMS JULIE , ZIEGLE JANET , DAILEY DAVID , RHODES MICHAEL , BELLON LAURENT R , EDDINS SUSAN , SPRAGUE ARIAN , WEBSTER MARION N , WANG YU N , SPIER EUGENE G , STEVENS JUNKO , LIVAK KENNETH J , KOEHLER RYAN T , DE LA VEGA FRANCISCO M
IPC分类号： G01N21/78 , B01L3/00 , B01L3/14 , C07H21/04 , C12M1/00 , C12N15/09 , C12P19/34 , C12Q1/68 , G01N20060101 , G01N27/62 , G01N30/88 , G01N33/48 , G01N33/50 , G01N35/00 , G06F20060101 , G06F17/00 , G06F17/30 , G06Q30/06 , G16B45/00 , H04L12/16 , G06F17/60 , G06F19/00
摘要： Methods and systems for ordering assays which detect SNPs or gene expression are provided. The methods use PCR and RT-PCR procedures. Collections of stoc k assays are assembled using pre-and post -manufacturing quality control procedures and made available to consumers via the Internet. In addition, custom assays are prepared upon order from the consumer and these assays are also prepared using pre-and post-manufacturing quality control procedures. T h e assays are then delivered to the consumer.

28. 发明专利

AU2007201863A1 Determination of a genotype of an amplification product at multiple allelic sites 未知
公开(公告)号：AU2007201863A1
公开(公告)日：2007-05-17
申请号：AU2007201863
申请日：2007-04-27
申请人： APPLERA CORP
发明人： LIVAK KENNETH J , GOODSAID FEDERICO
IPC分类号： C12Q1/68

29. 发明专利

AT329056T 未知
公开(公告)号：AT329056T
公开(公告)日：2006-06-15
申请号：AT00906924
申请日：2000-01-14
申请人： APPLERA CORP
发明人： LIVAK KENNETH J , EGHOLM MICHAEL , HUNKAPILLER MICHAEL W
IPC分类号： G01N33/483 , C12N15/09 , C12Q1/68 , C12Q1/6816 , C12Q1/6818 , G01N33/15 , G01N33/53 , G01N33/533 , G01N33/566 , G01N33/58
摘要： Binary probe and clamp compositions conduct methods for target hybridization detection. Where the probe is a substrate for exonuclease cleavage, the composition provides quantitation and detection of PCR products, by real-time and end-point measurements. Where the probe is an amplification primer, the composition provides an improved method for labelling and detection of PCR products. Probes and clamps may be labelled with fluorescent dyes, quenchers, hybridization-stabilizing moieties, chemiluminescent dyes, and affinity ligands. Clamps may be nucleic acid analogs, such as 2-aminoethylglycine PNA.

30. 发明专利

AU2009225303A1 Methods and compositions for detecting targets 未知
公开(公告)号：AU2009225303A1
公开(公告)日：2009-10-29
申请号：AU2009225303
申请日：2009-10-09
申请人： APPLERA CORP
发明人： HUNKAPILLER MICHAEL W , SPIER EUGENE G , ANDERSEN MARK R , LIVAK KENNETH J , WENZ MICHAEL H
IPC分类号： C12Q1/68 , C07H21/00 , C07H21/02 , C07H21/04 , C12P19/34 , C12Q20060101

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式