专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明专利

JP2009017882A Binary probe and clamp composition and method, for target hybridization detection 审中-公开
标题翻译：二进制和夹钳组合物和方法，用于目标杂交检测
公开(公告)号：JP2009017882A
公开(公告)日：2009-01-29
申请号：JP2008195452
申请日：2008-07-29
申请人： Applera Corp , アプレラコーポレイション
发明人： LIVAK KENNETH J , EGHOLM MICHAEL , HUNKAPILLER MICHAEL W
IPC分类号： C12Q1/68 , G01N33/483 , C12N15/09 , G01N21/76 , G01N21/78 , G01N33/15 , G01N33/53 , G01N33/533 , G01N33/566 , G01N33/58
CPC分类号： C12Q1/6816 , C12Q1/6818 , C12Q2549/126 , C12Q2537/119 , C12Q2525/179
摘要： PROBLEM TO BE SOLVED: To provide binary probe and clump compositions useful for performing methods for target hybridization detection. SOLUTION: Where the probe is a substrate for exonuclease cleavage, the composition provides quantitation and detection of PCR products, by real-time and end-point measurements. Where the probe is an amplification primer, the composition provides an improved method for labeling and detection of PCR products. Probes and clamps may be labeled with fluorescent dyes, quenchers, hybridization-stabilizing moieties, chemiluminescent dyes, and affinity ligands. Clamps may be nucleic acid analogs, such as 2-aminoethylglycine PNA. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供用于执行靶杂交检测方法的二元探针和团块组合物。解决方案：当探针是外切核酸酶切割的底物时，组合物通过实时和终点测量提供PCR产物的定量和检测。当探针是扩增引物时，组合物提供了PCR产物的标记和检测的改进方法。探针和夹具可以用荧光染料，猝灭剂，杂交稳定部分，化学发光染料和亲和配体标记。夹具可以是核酸类似物，例如2-氨基乙基甘氨酸PNA。版权所有（C）2009，JPO＆INPIT

2. 发明专利

JP2005160489A Binary probe and clamp composition and method for target hybridization detection 审中-公开
标题翻译：二次探针和夹钳组合物和方法进行目标杂交检测
公开(公告)号：JP2005160489A
公开(公告)日：2005-06-23
申请号：JP2005047966
申请日：2005-02-23
申请人： Applera Corp , アプレラコーポレイション
发明人： LIVAK KENNETH J , EGHOLM MICHAEL , HUNKAPILLER MICHAEL W
IPC分类号： G01N33/483 , C12N15/09 , C12Q1/68 , G01N33/15 , G01N33/53 , G01N33/533 , G01N33/566 , G01N33/58
CPC分类号： C12Q1/6816 , C12Q1/6818 , C12Q2549/126 , C12Q2537/119 , C12Q2525/179
摘要： PROBLEM TO BE SOLVED: To provide methods for target hybridization detection by binary probe and clamp compositions. SOLUTION: Where the probe is a substrate for exonuclease cleavage, the composition provides quantitation and detection of PCR products, by real-time and end-point measurements. Where the probe is an amplification primer, the composition provides an improved method for labelling and detection of PCR products. Probes and clamps may be labelled with fluorescent dyes, quenchers, hybridization-stabilizing moieties, chemiluminescent dyes, and affinity ligands. Clamps may be nucleic acid analogs, such as 2-aminoethylglycine PNA. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供通过二元探针和夹具组合物进行靶杂交检测的方法。解决方案：当探针是外切核酸酶切割的底物时，组合物通过实时和终点测量提供PCR产物的定量和检测。当探针是扩增引物时，组合物提供了PCR产物的标记和检测的改进方法。探针和夹具可以用荧光染料，猝灭剂，杂交稳定部分，化学发光染料和亲和配体标记。夹具可以是核酸类似物，例如2-氨基乙基甘氨酸PNA。版权所有（C）2005，JPO＆NCIPI

3. 发明专利

JP2006055177A Template-dependent ligation using pna-dna chimeric probe 审中-公开
标题翻译：使用PNA-DNA CHIMERIC PROBE的模板依赖关系
公开(公告)号：JP2006055177A
公开(公告)日：2006-03-02
申请号：JP2005329510
申请日：2005-11-14
申请人： Applera Corp , アプレラコーポレイション
发明人： EGHOLM MICHAEL , CHEN CAIFU
IPC分类号： C12N15/09 , G01N21/78 , C12Q1/68
CPC分类号： C12Q1/6862
摘要： PROBLEM TO BE SOLVED: To provide chimeric molecules of PNA (peptide nucleic acid) and DNA monomer units, and their use in ligation methods to generate ligation products. SOLUTION: Disclosed is a method for producing a template-dependent ligation product, wherein the method comprises a process of enzymatically linking a PNA-DNA chimeric probe to a second probe in the presence of a template nucleic acid and ligase, wherein the chimeric probe has a PNA part and a DNA part and the DNA part has at least two nucleotides and 3' hydroxy terminus or 5' hydroxy terminus, wherein the chimeric probe and the second probe are respectively hybridized to the template nucleic acid and are adjacent each other, and wherein at least one part of the PNA part is hybridized to a template and the second probe is a PNA-DNA chimera or oligonucleotide. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：提供PNA（肽核酸）和DNA单体单元的嵌合分子，以及它们在连接方法中的用途以产生连接产物。解决方案：公开了一种制备模板依赖性连接产物的方法，其中该方法包括在模板核酸和连接酶存在下将PNA-DNA嵌合探针酶催化连接到第二探针的方法，其中嵌合探针具有PNA部分和DNA部分，DNA部分具有至少两个核苷酸和3'羟基末端或5'羟基末端，其中嵌合探针和第二探针分别与模板核酸杂交并与每个并且其中至少一部分PNA部分与模板杂交，第二探针是PNA-DNA嵌合体或寡核苷酸。版权所有（C）2006，JPO＆NCIPI

4. 发明申请

WO0194638A3 ASYNCHRONOUS PRIMED PCR 审中-公开
标题翻译：异步PRIMED PCR
公开(公告)号：WO0194638A3
公开(公告)日：2003-07-10
申请号：PCT/US0118464
申请日：2001-06-06
申请人： APPLERA CORP
发明人： CHEN CAIFU , EGHOLM MICHAEL , HAFF LAWRENCE
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N33/566
CPC分类号： C12Q1/686 , C12Q2561/101 , C12Q2527/113 , C12Q2527/107
摘要： An asynchronous thermal cycling protocol for nucleic acid amplification uses two primers with thermal melting temperatures different by about 10 to 30 DEG C. After the higher melting primer has annealed and polymerase mediated extension, the uncopied, single-stranded target sequence may be hybridized and detected by a probe. DNA probes may be cleaved by the exonuclease activity of a polymerase. The probe may be a non-cleaving analog such as PNA. When a probe is labelled with a reporter dye and a quencher selected to undergo energy transfer, e.g. FRET, fluorescence from the reporter dye may be effectively quenched when the probe is unbound. Upon hybridization of the probe to complementary target or upon cleavage while bound to target, the reporter dye is no longer quenched, resulting in a detectable amount of fluorescence. The second, lower-melting primer may be annealed and extended to generate a double-stranded nucleic acid. Amplification may be monitored in real time, including each cycle, or at the end point. The asynchronous PCR thermal cycling protocol can generate a preponderance of the PCR amplicon in single-stranded form by repetition at the end of the protocol of annealing and extension of the higher melting primer.
摘要翻译：用于核酸扩增的异步热循环方案使用两种引物，其热解温度不同于约10至30℃。在较高熔点的引物退火和聚合酶介导的延伸之后，未结合的单链靶序列可能被杂交和检测通过探针。 DNA探针可能被聚合酶的外切核酸酶活性切割。探针可以是非断裂的类似物，例如PNA。当探针用报告染料和选择进行能量转移的猝灭剂标记时，例如，当探针未结合时，来自报告染料的荧光可以被有效地淬灭。当探针与互补靶物杂交时或在与靶标结合时进行切割时，报道染料不再猝灭，导致可检测量的荧光。可以将第二种较低熔点的引物退火并延伸以产生双链核酸。可以实时监测放大，包括每个周期，或在终点。异构PCR热循环方案可以在退火和延伸较高熔点引物的方案结束时通过重复生成单链形式的PCR扩增子的优势。

5. 发明申请

WO0194638A8 ASYNCHRONOUS PRIMED PCR 审中-公开
标题翻译：异步PRIMED PCR
公开(公告)号：WO0194638A8
公开(公告)日：2002-04-11
申请号：PCT/US0118464
申请日：2001-06-06
申请人： APPLERA CORP
发明人： CHEN CAIFU , EGHOLM MICHAEL , HAFF LAWRENCE
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N33/566
CPC分类号： C12Q1/686 , C12Q2561/101 , C12Q2527/113 , C12Q2527/107
摘要： An asynchronous thermal cycling protocol for nucleic acid amplification uses two primers with thermal melting temperatures different by about 10 to 30 DEG C. After the higher melting primer has annealed and polymerase mediated extension, the uncopied, single-stranded target sequence may be hybridized and detected by a probe. DNA probes may be cleaved by the exonuclease activity of a polymerase. The probe may be a non-cleaving analog such as PNA. When a probe is labelled with a reporter dye and a quencher selected to undergo energy transfer, e.g. FRET, fluorescence from the reporter dye may be effectively quenched when the probe is unbound. Upon hybridization of the probe to complementary target or upon cleavage while bound to target, the reporter dye is no longer quenched, resulting in a detectable amount of fluorescence. The second, lower-melting primer may be annealed and extended to generate a double-stranded nucleic acid. Amplification may be monitored in real time, including each cycle, or at the end point. The asynchronous PCR thermal cycling protocol can generate a preponderance of the PCR amplicon in single-stranded form by repetition at the end of the protocol of annealing and extension of the higher melting primer.
摘要翻译：用于核酸扩增的异步热循环方案使用两种引物，其热解温度不同于约10至30℃。在较高熔点的引物退火和聚合酶介导的延伸之后，未结合的单链靶序列可能被杂交和检测通过探针。 DNA探针可能被聚合酶的外切核酸酶活性切割。探针可以是非断裂的类似物，例如PNA。当探针用报告染料和选择进行能量转移的猝灭剂标记时，例如，当探针未结合时，来自报告染料的荧光可以被有效地淬灭。当探针与互补靶物杂交时或在与靶标结合时进行切割时，报道染料不再猝灭，导致可检测量的荧光。可以将第二种较低熔点的引物退火并延伸以产生双链核酸。可以实时监测放大，包括每个周期，或在终点。异构PCR热循环方案可以在退火和延伸较高熔点引物的方案结束时通过重复生成单链形式的PCR扩增子的优势。

6. 发明申请

WO0210182A3 METHODS FOR ISOLATING ONE STRAND OF A DOUBLE-STRANDED NUCLEIC ACID 审中-公开
标题翻译：用于分离双重条纹核酸的一条条的方法
公开(公告)号：WO0210182A3
公开(公告)日：2003-05-30
申请号：PCT/US0122782
申请日：2001-07-18
申请人： APPLERA CORP , CHIESA CLAUDIA , SCHROTH GARY P , EGHOLM MICHAEL
发明人： CHIESA CLAUDIA , SCHROTH GARY P , EGHOLM MICHAEL
IPC分类号： C12N15/09 , C12N15/10 , C07H21/00 , C07H1/08 , C12Q1/68
CPC分类号： C12N15/1006 , C12N15/10 , Y10T436/143333
摘要： The present invention provides methods and kits for isolating one strand of a double-stranded target nucleic acid. The method capitalizes on the differences in the kinetics and thermodynamic stabilities between conventional DNA/DNA, DNA/RNA and RNA/RNA duplexes and heteroduplexes in which one strand of the heteroduplexe is a nucleobase polymer having a net positively charged or net neutral backbone, such as a PNA.
摘要翻译：本发明提供了用于分离双链靶核酸的一条链的方法和试剂盒。该方法利用常规DNA / DNA，DNA / RNA和RNA / RNA双链体之间的动力学和热力学稳定性的差异，其中异源双链体的一条链是具有净正电荷或净中性主链的核碱基聚合物，作为PNA。

7. 发明申请

WO0127326A3 TEMPLATE-DEPENDENT LIGATION WITH PNA-DNA CHIMERIC PROBES 审中-公开
标题翻译：具有PNA-DNA半导体探针的模板依赖性结构
公开(公告)号：WO0127326A3
公开(公告)日：2002-05-10
申请号：PCT/US0027730
申请日：2000-10-06
申请人： APPLERA CORP
发明人： EGHOLM MICHAEL , CHEN CAIFU
IPC分类号： G01N21/78 , C12N15/09 , C12Q1/68 , C07K14/00
CPC分类号： C12Q1/6862
摘要： The invention provides methods, kits, and compositions for ligation of PNA-DNA chimeric probes and oligonucleotides when they are hybridized adjacently to template nucleic acids using ligases and ligation reagents. Structural requirements of the chimeras for ligation include 5 to 15 contiguous PNA monomer units, 2 or more contiguous nucleotides, and a 3' hydroxyl or 5' hydroxyl terminus. The chimera and/or oligonucleotide may be labelled with fluorescent dyes or other labels. The methods include, for example, oligonucleotide-ligation assays (OLA) and single nucleotide polymorphism detection.
摘要翻译：当使用连接酶和连接试剂将它们与模板核酸相互杂交时，本发明提供用于连接PNA-DNA嵌合探针和寡核苷酸的方法，试剂盒和组合物。用于连接的嵌合体的结构要求包括5至15个连续的PNA单体单元，2个或更多个连续核苷酸，以及3'羟基或5'羟基末端。嵌合体和/或寡核苷酸可以用荧光染料或其他标记物标记。所述方法包括例如寡核苷酸连接测定（OLA）和单核苷酸多态性检测。

8. 发明专利

AU6630200A Polymerase extension at 3' terminus of pna-dna chimera 未知
公开(公告)号：AU6630200A
公开(公告)日：2001-03-13
申请号：AU6630200
申请日：2000-08-09
申请人： APPLERA CORP
发明人： EGHOLM MICHAEL , CHEN CAIFU
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N21/78 , G01N33/566 , G01N33/58

9. 发明专利

DE60028534T2 未知
公开(公告)号：DE60028534T2
公开(公告)日：2007-06-06
申请号：DE60028534
申请日：2000-01-14
申请人： APPLERA CORP
发明人： LIVAK J , EGHOLM MICHAEL , HUNKAPILLER W
IPC分类号： C12Q1/68 , G01N33/483 , C12N15/09 , C12Q1/6816 , C12Q1/6818 , G01N33/15 , G01N33/53 , G01N33/533 , G01N33/566 , G01N33/58
摘要： Binary probe and clamp compositions conduct methods for target hybridization detection. Where the probe is a substrate for exonuclease cleavage, the composition provides quantitation and detection of PCR products, by real-time and end-point measurements. Where the probe is an amplification primer, the composition provides an improved method for labelling and detection of PCR products. Probes and clamps may be labelled with fluorescent dyes, quenchers, hybridization-stabilizing moieties, chemiluminescent dyes, and affinity ligands. Clamps may be nucleic acid analogs, such as 2-aminoethylglycine PNA.

10. 发明专利

DE60020124T2 未知
公开(公告)号：DE60020124T2
公开(公告)日：2006-01-19
申请号：DE60020124
申请日：2000-08-09
申请人： APPLERA CORP
发明人： EGHOLM MICHAEL , CHEN CAIFU
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N21/78 , G01N33/566 , G01N33/58

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式