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1. 发明专利

CA2466821A1 DIGITAL ASSAY 未知
公开(公告)号：CA2466821A1
公开(公告)日：2003-06-05
申请号：CA2466821
申请日：2002-11-21
申请人： APPLERA CORP
发明人： REED MARK , VANN CHARLES S , LAO KAI QIN
IPC分类号： G01N33/53 , A61K20060101 , C07H21/02 , C12N9/22 , C12P19/34 , C12Q1/68 , G01N33/543 , G01N33/566
摘要： The invention relates to methods and compositions for the detection of targe ts in a sample.

2. 发明申请

WO2007011903A3 ANALYZING MESSENGER RNA AND MICRO RNA IN THE SAME REACTION MIXTURE 审中-公开
标题翻译：在同一反应混合物中分析信使RNA和微RNA
公开(公告)号：WO2007011903A3
公开(公告)日：2007-07-12
申请号：PCT/US2006027757
申请日：2006-07-17
申请人： APPLERA CORP , LAO KAI QIN , STRAUS NEIL A
发明人： LAO KAI QIN , STRAUS NEIL A
IPC分类号： C12P19/34 , C07K17/00
CPC分类号： C12Q1/686 , C12Q1/6809 , C12Q1/6844 , C12Q1/6848 , C12Q1/6853 , C12Q2549/101 , C12Q2537/143 , C12Q2527/107 , C12Q2525/301 , C12Q2525/207 , C12Q2533/101
摘要： The present teachings provide methods, compositions, and kits for performing primer extension reactions on at least two target polynucleotides in the same reaction mixture. In some embodiments, a reverse transcription reaction is performed on a first target polynucleotide with a hot start primer comprising a self-complementary stem and a loop, and extension products form at high temperatures but extension products form less so at low temperatures since the self-complementary stem of the hot start primer prevents hybridization of the target specific region to the target. However, non-hot start primers with free target specific regions can hybridize to their corresponding targets at the low temperature and extension can happen at the low temperature.
摘要翻译：本教导提供了用于在相同反应混合物中对至少两个靶多核苷酸进行引物延伸反应的方法，组合物和试剂盒。在一些实施方案中，用包含自身互补茎和环的热启动引物对第一靶多核苷酸进行逆转录反应，并且在高温下形成延伸产物，但延伸产物在低温下形成较少，热启动引物的互补茎阻止靶特异性区域与靶标杂交。然而，具有游离靶标特异性区域的非热启动引物可以在低温下与其相应的靶标杂交并且可以在低温下延伸。

3. 发明申请

WO2007044066A3 METHODS FOR NORMALIZING AND FOR IDENTIFYING SMALL NUCLEIC ACIDS 审中-公开
标题翻译：用于正常化和鉴定小核酸的方法
公开(公告)号：WO2007044066A3
公开(公告)日：2008-01-10
申请号：PCT/US2006012025
申请日：2006-03-29
申请人： APPLERA CORP , LAO KAI QIN , STRAUS NEIL A , BURNS JOHN W
发明人： LAO KAI QIN , STRAUS NEIL A , BURNS JOHN W
IPC分类号： C12Q1/68 , C07H21/02
CPC分类号： C12Q1/6855 , C12Q1/6851 , C12Q2535/138 , C12Q2525/121 , C12Q2525/301
摘要： The present teachings are generally directed to methods for normalizing at least one species of small nucleic acid that is present in a population of small nucleic acid species, wherein the relative concentration of at least one small nucleic acid species is substantially greater than the relative concentration of at least one other small nucleic acid species in the population. At least one small nucleic acid species is normalized using a multiplicity of primers comprising degenerate sequences. In some embodiments, a small nucleic acid species is identified by inserting at least part of an extension product from a normalized population into a vector and subsequently sequencing the insert. In some embodiments, a small nucleic acid species is identified by determining the sequence of at least part of an extension product.
摘要翻译：本教导通常涉及用于归一化存在于小核酸物种群中的至少一种小核酸的方法，其中至少一种小核酸物质的相对浓度基本上大于群体中至少一种其他小核酸物种。使用包含简并序列的多个引物将至少一个小核酸物质归一化。在一些实施方案中，通过将来自标准化群体的至少部分延伸产物插入载体并随后对插入物进行测序来鉴定小核酸物种。在一些实施方案中，通过确定延伸产物的至少一部分的序列来鉴定小核酸物种。

4. 发明申请

WO03045310A8 DIGITAL ASSAY 审中-公开
公开(公告)号：WO03045310A8
公开(公告)日：2004-05-27
申请号：PCT/US0237499
申请日：2002-11-21
申请人： APPLERA CORP , VANN CHARLES S , LAO KAI QIN , REED MARK
发明人： VANN CHARLES S , LAO KAI QIN , REED MARK
IPC分类号： G01N33/53 , A61K20060101 , C07H21/02 , C12N9/22 , C12P19/34 , C12Q1/68 , G01N33/543 , G01N33/566
CPC分类号： C12Q1/6816 , B82Y5/00 , B82Y10/00 , C12Q1/6827 , C12Q2563/155 , C12Q2561/125 , C12Q2545/114 , C12Q2565/102 , C12Q2563/143
摘要： The invention relates to methods and compositions for the detection of targets in a sample.

5. 发明公开

EP1448800A4 DIGITAL ASSAY 审中-公开
标题翻译：数码测定
公开(公告)号：EP1448800A4
公开(公告)日：2007-05-16
申请号：EP02797138
申请日：2002-11-21
申请人： APPLERA CORP
发明人： VANN CHARLES S , LAO KAI QIN , REED MARK
IPC分类号： C12Q1/68 , G01N33/53 , A61K20060101 , C07H21/02 , C12N9/22 , C12P19/34 , G01N33/50 , G01N33/543 , G01N33/566
CPC分类号： C12Q1/6816 , B82Y5/00 , B82Y10/00 , C12Q1/6827 , C12Q2563/155 , C12Q2561/125 , C12Q2545/114 , C12Q2565/102 , C12Q2563/143
摘要： The invention relates to methods and compositions for the detection of targets in a sample.

6. 发明专利

CA2591652A1 ID-TAG COMPLEXES, ARRAYS, AND METHODS OF USE THEREOF 未知
公开(公告)号：CA2591652A1
公开(公告)日：2006-06-22
申请号：CA2591652
申请日：2005-12-06
申请人： APPLERA CORP
发明人： STRAUS NEIL A , LAO KAI QIN , SCHROTH GARY P
IPC分类号： C12Q1/68

7. 发明专利

AU2003233495A8 Universal-tagged oligonucleotide primers and methods of use 未知
公开(公告)号：AU2003233495A8
公开(公告)日：2003-12-02
申请号：AU2003233495
申请日：2003-05-07
申请人： APPLERA CORP
发明人： SCHROTH GARY , LAO KAI QIN , KOEHLER RYAN T , SCAFE CHARLES , CHEN CAIFU
IPC分类号： C07H21/00 , C12Q1/68 , C07H21/04 , C12P19/34
摘要： The present invention relates to universal-tagged oligonucleotide primers, and to methods of using the primers for amplifying the genome.

8. 发明专利

DE602005007874D1 未知
公开(公告)号：DE602005007874D1
公开(公告)日：2008-08-14
申请号：DE602005007874
申请日：2005-03-24
申请人： APPLERA CORP
发明人： LAO KAI QIN , REED MARK T
IPC分类号： C12Q1/68

9. 发明专利

AU2002362013A1 DIGITAL ASSAY 未知
公开(公告)号：AU2002362013A1
公开(公告)日：2003-06-10
申请号：AU2002362013
申请日：2002-11-21
申请人： APPLERA CORP
发明人： VANN CHARLES S , LAO KAI QIN , REED MARK
IPC分类号： G01N33/53 , A61K20060101 , C07H21/02 , C12N9/22 , C12P19/34 , C12Q1/68 , G01N33/543 , G01N33/566
摘要： The invention relates to kits for the detection of targets in a sample. In certain embodiments, methods for quantitating a target are provided. In certain embodiments, the methods comprise forming a reaction mixture comprising: a sample possibly containing the target; a codeable label; one or more target-specific probes, wherein each target-specific probe binds specifically to the target under selective binding conditions; and a separating moiety. In certain embodiments, the methods further comprise treating the reaction mixture with a ligase under reaction conditions such that a detectable complex is produced when the target is present, and such that a detectable complex is not produced when the target is absent, and wherein the detectable complex comprises the codeable label, the target-specific probe, and the separating moiety. In certain embodiments, the methods further comprise separating the detectable complex from codeable labels that are not included in the detectable complex, and quantitating the target by counting the number of codeable labels.

10. 发明申请

WO2008076842A3 SEQUENCING METHODS 审中-公开
标题翻译：序列方法
公开(公告)号：WO2008076842A3
公开(公告)日：2008-11-27
申请号：PCT/US2007087472
申请日：2007-12-13
申请人： APPLERA CORP , LAO KAI QIN , STRAUS NEIL A
发明人： LAO KAI QIN , STRAUS NEIL A
IPC分类号： C12P19/34 , C07H21/04 , C12M1/34 , C12M1/40 , C12N11/18 , C12Q1/66 , C12Q1/68
CPC分类号： C12Q1/6872 , C12Q2537/143 , C12Q2535/101
摘要： The present teachings provide methods and compositions for sequencing one or more target nucleic acids. High levels of multiplexing are provided by the use of an emulsion PCR comprising primer-immobilized beads. The resulting reaction products can be sequenced by any of a variety of mobility-dependent analytical techniques, such as mass spectrometry. In some embodiments, a first collection of amplification products on a first collection of beads are transferred to a second collection of beads. In some embodiments, a first collection of amplification products on a first collection of beads is amplified in a rolling circle amplification reaction. The present teachings also provide compositions, kits, and devices for performing and sequencing the products of the emulsion amplification reactions as described herein.
摘要翻译：本教导提供了用于测序一种或多种靶核酸的方法和组合物。通过使用包含引物固定的珠粒的乳液PCR提供了高水平的复用。所得到的反应产物可以通过各种迁移依赖性分析技术如质谱法中的任何一种进行测序。在一些实施方案中，将第一集合珠上的扩增产物的第一集合转移到第二集合珠。在一些实施方案中，在滚动圆扩增反应中扩增第一集合珠上的扩增产物的第一集合。本教导还提供用于对本文所述的乳液扩增反应的产物进行和测序的组合物，试剂盒和装置。

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