会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 24. 发明公开
    • 합성 재조합효소 유전자 및 이를 이용한 선발표지 유전자 제거용 식물형질전환 벡터
    • 用于消除选择性标记基因的改性位点特异性重组体和植物转化载体
    • KR1020130054544A
    • 2013-05-27
    • KR1020110119972
    • 2011-11-17
    • 대한민국(농촌진흥청장)
    • 우희종신공식임명호권순종서석철이진형
    • A01H5/00C12N15/52C12N15/82
    • C12N15/8222C12N15/52C12N15/8205C12N15/8271
    • PURPOSE: A plant transformation vector, pCMF, for removing a selective marker is provided to enable easy modification of the vector and transduction of a target gene, and to develop marker-free GM(genetically modified) crops. CONSTITUTION: A site-specific recombinase gene, mFLP, has a base in sequence number 1. A vector for transformation in a plant contains the gene, mFLP. The vector contains a stress-induced promoter and recombinase recognition site. The vector is able to remove a selective marker by induction of mFP expression and self-cleavage due to stress. The vector contains MCS(multi-cloning site). A plant is transformed with the vector. Agrobacterium tumefaciens LBA4404 is transformed by the vector and is used as a plant carrier. A transgenic plant is prepared by transforming with the vector and is able to remove the selective marker.
    • 目的:提供用于去除选择性标记的植物转化载体pCMF,以便容易地修饰靶基因的载体和转导,并开发无标记的转基因(转基因)作物。 构成:位点特异性重组酶基因mFLP具有序列号1的碱基。用于在植物中转化的载体含有基因mFLP。 载体含有应激诱导的启动子和重组酶识别位点。 该载体能够通过诱导mFP表达和由于应激而自身切割而去除选择性标记。 载体包含MCS(多克隆位点)。 用载体转化植物。 根癌土壤杆菌LBA4404被载体转化并用作植物载体。 通过用载体转化并能够除去选择性标记来制备转基因植物。