专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

21. 发明授权

US06184344B2 Synthesis of proteins by native chemical ligation 失效
标题翻译：通过天然化学结合合成蛋白质
公开(公告)号：US06184344B2
公开(公告)日：2001-02-06
申请号：US08945997
申请日：1998-02-12
申请人： Stephen B. H. Kent , Tom W. Muir , Philip E. Dawson
发明人： Stephen B. H. Kent , Tom W. Muir , Philip E. Dawson
IPC分类号： C07K102
CPC分类号： C07K1/04 , C07K1/026
摘要： Proteins of moderate size having native peptide backbones are produced by a method of native chemical ligation. Native chemical ligation employs a chemoselective reaction of two unprotected peptide segments to produce a transient thioester-linked intermediate. The transient thioester-linked intermediate then spontaneously undergoes a rearrangement to provide the full length ligation product having a native peptide bond at the ligation site. Full length ligation products are chemically identical to proteins produced by cell free synthesis. Full length ligation products may be refolded and/or oxidized, as allowed, to form native disulfide-containing protein molecules. The technique of native chemical ligation is employable for chemically synthesizing full length proteins.
摘要翻译：通过天然化学连接的方法产生具有天然肽骨架的中等体积的蛋白质。天然化学连接采用两个未保护肽段的化学选择性反应产生瞬时硫酯连接的中间体。然后瞬时硫酯连接的中间体自发地进行重排，以在连接位点提供具有天然肽键的全长连接产物。全长连接产物与通过无细胞合成产生的蛋白质化学相同。如所允许的，全长连接产物可以重折叠和/或氧化以形成天然二硫化物的蛋白质分子。天然化学连接的技术可用于化学合成全长蛋白质。

22. 发明授权

US5620844A Assays for detecting hepatitis B virus envelope antigens or antibodies thereto and diagnostic test kits for use in performing the assays 失效
标题翻译：用于检测乙型肝炎病毒包膜抗原或其抗体的检测方法以及用于进行检测的诊断检测试剂盒
公开(公告)号：US5620844A
公开(公告)日：1997-04-15
申请号：US57200
申请日：1993-05-03
申请人： Alexander R. Neurath , Stephen B. H. Kent
发明人： Alexander R. Neurath , Stephen B. H. Kent
IPC分类号： A61K9/127 , A61K39/00 , A61K47/48 , C07K14/02 , C07K16/08 , G01N33/576 , C12Q1/70 , A61K38/08 , G01N33/53 , G01N33/537
CPC分类号： C07K14/005 , A61K47/48176 , A61K47/48315 , A61K47/48815 , A61K9/1271 , B82Y5/00 , C07K16/082 , G01N33/5761 , G01N33/5764 , A61K39/00 , C12N2730/10122
摘要： A hepatitis B vaccine containing a peptide with an amino acid chain of at least six consecutive amino acids within the pre-S gene coded region of the envelope of hepatitis B virus. The vaccine being free of an amino acid sequence corresponding to the naturally occurring envelope proteins of hepatitis B virus and a physiologically acceptable diluent. The peptide being free or linked to a carrier. The carrier being a conventional carrier or a novel carrier including a lipid vesicle stabilized by cross-linking and having covalently bonded active sites on the outer surface thereon. Such novel carrier being useful not only to link the novel peptide containing an amino acid chain with amino acids within the pre-S gene coded region of the surface antigen of hepatitis B virus, but can also be used to bind synthetic peptide analogues of other viral proteins, as well as bacterial, allergen and parasitic proteins of man and animals. The peptides of the invention can be utilized in diagnostics for the detection of antigens and antibodies.
摘要翻译：一种乙型肝炎疫苗，其含有乙型肝炎病毒包膜前S基因编码区内具有至少六个连续氨基酸的氨基酸链的肽。该疫苗不含对应于乙型肝炎病毒的天然存在的包膜蛋白的氨基酸序列和生理上可接受的稀释剂。肽与载体无关或连接。载体是常规载体或包含通过交联稳定并在其外表面上具有共价键合的活性位点的脂质囊泡的新型载体。这种新型载体不仅可用于将含有氨基酸链的新肽与乙型肝炎病毒表面抗原前S基因编码区内的氨基酸连接，还可用于结合其他病毒的合成肽类似物蛋白质，以及人和动物的细菌，变应原和寄生蛋白。本发明的肽可用于诊断抗原和抗体的检测。

23. 发明授权

US4548904A Protein sequencing method 失效
标题翻译：蛋白测序法
公开(公告)号：US4548904A
公开(公告)日：1985-10-22
申请号：US446579
申请日：1982-12-03
申请人： Stephen B. H. Kent , James J. L'Italien
发明人： Stephen B. H. Kent , James J. L'Italien
IPC分类号： G01N33/68
CPC分类号： G01N33/6824 , Y10T436/2525
摘要： A protein sequencing method utilizing a composition of matter which comprises a D-C-B-A reagent wherein A is a moiety which can react with and bind to a terminal amino acid of a protein and can result in removal of the terminal amino amino acid, B is a moiety which provides steric separation between C and A, C is a nucleophilic moiety which can be detected, and D is a moiety which protects the C moiety from degradation or other modification, and is labile in acidic media and stable in neutral or basic media. The protein sequencing method and reagent can be used in micro sequencing apparatus.
摘要翻译：使用包含DCBA试剂的物质组合物的蛋白质测序方法，其中A是可以与蛋白质的末端氨基酸反应并结合到蛋白质的末端氨基酸并可导致末端氨基酸的去除的部分，B是其部分提供C和A之间的空间分离，C是可以检测的亲核部分，D是保护C部分不被降解或其它修饰的部分，并且在酸性介质中是不稳定的，在中性或基本介质中是稳定的。蛋白测序方法和试剂可用于微测序仪。

24. 发明授权

US07094871B2 Solid phase native chemical ligation of unprotected or N-terminal cysteine protected peptides in aqueous solution 失效
公开(公告)号：US07094871B2
公开(公告)日：2006-08-22
申请号：US09987655
申请日：2001-11-15
申请人： Lynne Canne , Stephen B. H. Kent , Reyna Simon
发明人： Lynne Canne , Stephen B. H. Kent , Reyna Simon
IPC分类号： C07K1/02
CPC分类号： C07K1/086 , C07K1/023 , C07K1/026 , C07K1/04 , C07K1/047
摘要： The present invention provides methods, apparatus and kits for synthesizing assembled peptides and proteins on a solid phase with sequential ligation of three or more unprotected peptide segments using chemoselective and mild ligation chemistries in aqueous solution. Also provided are methods of monitoring solid phase sequential ligation reactions using MALDI or electrospray ionization mass spectrometry of reaction products.

25. 发明授权

US06844161B2 Modular protein libraries and methods of preparation 失效
标题翻译：模块化蛋白质文库及其制备方法
公开(公告)号：US06844161B2
公开(公告)日：2005-01-18
申请号：US09144838
申请日：1998-08-31
申请人： Michael A. Siani , Jill Wilken , Reyna Simon , Stephen B. H. Kent
发明人： Michael A. Siani , Jill Wilken , Reyna Simon , Stephen B. H. Kent
IPC分类号： A61K38/00 , C07K1/04 , C07K14/52 , C12Q1/00 , C07K14/00 , G01N33/53
CPC分类号： C07K14/523 , A61K38/00 , C07K1/04 , C07K1/047 , C07K14/522
摘要： Novel proteins and protein libraries are disclosed. The proteins possess one or more functional protein modules from different parent protein molecules. The proteins and protein libraries are exemplified by the preparation of cross-over chemokines that contain various combinations of peptide segments derived from RANTES, SDS-1 and vMIP-I and to vMIP-II. The proteins and libraries are extremely pure and can be provided in non-limiting high yields suitable for diagnostic and high-throughput screening assays.
摘要翻译：公开了新的蛋白质和蛋白质文库。蛋白质具有来自不同亲本蛋白质分子的一种或多种功能性蛋白质模块。蛋白质和蛋白质文库的实例是制备交叉趋化因子，其含有衍生自RANTES，SDS-1和vMIP-1以及vMIP-II的肽段的各种组合。蛋白质和文库是非常纯的，可以提供非限制性高产量，适用于诊断和高通量筛选分析。

26. 发明授权

US06228654B1 Methods for structure analysis of oligosaccharides 失效
标题翻译：寡糖结构分析方法
公开(公告)号：US06228654B1
公开(公告)日：2001-05-08
申请号：US08647405
申请日：1996-05-09
申请人： Brian T. Chait , Ying-Ming Zhao , Stephen B. H. Kent
发明人： Brian T. Chait , Ying-Ming Zhao , Stephen B. H. Kent
IPC分类号： G01N3348
CPC分类号： G01N33/66 , G01N2400/10 , Y10T436/143333 , Y10T436/24 , Y10T436/25125
摘要： A method of increasing the sensitivity and efficiency of MALDI-MS analysis of an oligosaccharide which comprises derivatization, prior to analysis by MALDI-MS, of said oligosaccharide by efficient ligation to a basic aminooxyacetylpeptide by oxime formation reaction to result in the formation of a glycoconjugate.
摘要翻译：提高寡糖的MALDI-MS分析的灵敏度和效率的方法，其包括通过MALDI-MS分析所述寡糖之前通过肟形成反应与碱性氨基氧乙酰肽有效连接导致糖缀合物的形成而衍生化。

27. 发明授权

US5854389A Process for preparing modified proteins 失效
标题翻译：制备修饰蛋白的方法
公开(公告)号：US5854389A
公开(公告)日：1998-12-29
申请号：US312662
申请日：1994-09-26
申请人： Stephen B. H. Kent , Martina Schnolzer-Rackwitz
发明人： Stephen B. H. Kent , Martina Schnolzer-Rackwitz
IPC分类号： C07K1/04 , C07K1/06 , C07K1/10 , C07K1/107 , C07K1/113 , C07K7/02 , C07K14/16 , C12N9/50
CPC分类号： C07K14/005 , C07K1/04 , C07K1/1075 , C07K1/1077 , C07K7/02 , C12N9/506 , C12N2740/16222 , Y02P20/582
摘要： Process for the preparation of modified proteins comprising the coupling of a first peptide segment having a haloacyl group at the N-terminus thereof with a second peptide segment having a carbonylthiol group at the C-terminus thereof are disclosed. Novel modified proteins produced by the process are also disclosed.
摘要翻译：公开了制备修饰蛋白质的方法，该修饰蛋白质包括其N-末端具有卤代酰基的第一肽段与C末端具有羰硫基的第二肽链的偶合。还公开了通过该方法产生的新型修饰的蛋白质。

28. 发明授权

US4861588A Pre-S gene coded peptide hepatitis B immunogens, vaccines, diagnostics, and synthetic lipid vesicle carriers 失效
标题翻译：前S基因编码肽乙型肝炎免疫原，疫苗，诊断和合成脂质囊泡载体
公开(公告)号：US4861588A
公开(公告)日：1989-08-29
申请号：US856522
申请日：1986-04-28
申请人： Alexander R. Neurath , Stephen B. H. Kent
发明人： Alexander R. Neurath , Stephen B. H. Kent
IPC分类号： C12P21/00 , A61K9/127 , A61K39/00 , A61K39/29 , A61K47/48 , C07K7/06 , C07K7/08 , C07K14/00 , C07K14/02 , C07K14/10 , C07K14/18 , C07K14/705 , C07K16/08 , C12Q1/16 , G01N33/53 , G01N33/576
CPC分类号： C07K14/005 , A61K47/48176 , A61K47/48315 , A61K47/48815 , A61K9/1271 , C07K16/082 , G01N33/5761 , A61K39/00 , C12N2730/10122 , Y10S930/223
摘要： A hepatitis B vaccine containing a peptide with an amino acid chain of at least six consecutive amino acids within the pre-S gene coded region of the envelope of hepatitis B virus. The vaccine being free of an amino acid sequence corresponding to the naturally occurring envelope proteins of hepatitis B virus and a physiologically acceptable diluent. The peptide being free or linked to a carrier. The carrier being a conventional carrier or a novel carrier including a lipid vesicle stabilized by cross-linking and having covalently bonded active sites on the outer surface thereon. Such novel carrier being useful not only to link the novel peptide containing an amino acid chain with amino acids within the pre-S gene coded region of the surface antigen of hepatitis B virus, but can also be used to bind synthetic peptide analogues of other viral proteins, as well as bacterial, allergen and parasitic proteins of man and animals. The peptides of the invention can be utilized in diagnostics for the detection of antigens and antibodies.
摘要翻译：一种乙型肝炎疫苗，其含有乙型肝炎病毒包膜前S基因编码区内具有至少六个连续氨基酸的氨基酸链的肽。该疫苗不含对应于乙型肝炎病毒的天然存在的包膜蛋白的氨基酸序列和生理上可接受的稀释剂。肽与载体无关或连接。载体是常规载体或包含通过交联稳定并在其外表面上具有共价键合的活性位点的脂质囊泡的新型载体。这种新型载体不仅可用于将含有氨基酸链的新肽与乙型肝炎病毒表面抗原前S基因编码区内的氨基酸连接，还可用于结合其他病毒的合成肽类似物蛋白质，以及人和动物的细菌，变应原和寄生蛋白。本发明的肽可用于诊断抗原和抗体的检测。

29. 发明授权

US4847080A Pre-S gene coded peptide hepatitis B immunogens, vaccines, diagnostics, and synthetic lipide vesicle carriers 失效
标题翻译：前S基因编码肽乙型肝炎免疫原，疫苗，诊断和合成脂质囊泡载体
公开(公告)号：US4847080A
公开(公告)日：1989-07-11
申请号：US698499
申请日：1985-02-05
申请人： Alexander R. Neurath , Stephen B. H. Kent
发明人： Alexander R. Neurath , Stephen B. H. Kent
IPC分类号： G01N33/576 , A61K9/127 , A61K39/00 , A61K39/29 , A61K39/385 , A61K47/48 , C07K7/04 , C07K14/00 , C07K14/02 , C07K14/10 , C07K14/18 , C07K14/435 , C07K16/08 , C07K19/00 , C12N15/00 , C12N15/09 , C12P21/00 , C12P21/08 , G01N33/543 , G01N33/577
CPC分类号： C07K14/005 , A61K47/48176 , A61K47/48315 , A61K47/48815 , A61K9/1271 , C07K16/082 , G01N33/5764 , A61K39/00 , C12N2730/10122 , Y10S930/221 , Y10S930/222 , Y10S930/223 , Y10S930/26 , Y10S930/27
摘要： A hepatitis B vaccine containing a peptide with an amino acid chain of at least six consecutive amino acids within the pre-S gene coded region of the envelope of hepatitis B virus. The vaccine being free of an amino acid sequence corresponding to the naturally occurring envelope proteins of hepatitis B virus and a physiologically acceptable diluent. The peptide being free or linked to a carrier. The carrier being a conventional carrier or a novel carrier including a lipid vesicle stabilized by cross-linking and having covalently bonded active sites on the outer surface thereon. Such novel carrier being useful not only to link the novel peptide containing an amino acid chain with amino acids within the pre-S gene coded region of the surface antigen of hepatitis B virus, but can also be used to bind synthetic peptide analogues of other viral proteins, as well as bacterial, allergen and parasitic proteins of man and animals. The peptides of the invention can be utilized in diagnostics for the detection of antigens and antibodies.V

30. 发明授权

US4668476A Automated polypeptide synthesis apparatus 失效
标题翻译：自动多肽合成仪
公开(公告)号：US4668476A
公开(公告)日：1987-05-26
申请号：US592638
申请日：1984-03-23
申请人： John Bridgham , Timothy G. Geiser , Michael W. Hunkapiller , Stephen B. H. Kent , Mark P. Marriott , Paul O. Ramstad , Eric S. Nordman
发明人： John Bridgham , Timothy G. Geiser , Michael W. Hunkapiller , Stephen B. H. Kent , Mark P. Marriott , Paul O. Ramstad , Eric S. Nordman
IPC分类号： C07K1/00 , B01J19/00 , C07K1/04 , C40B40/10 , C40B60/14 , C07K1/08
CPC分类号： C07K1/045 , B01J19/0046 , B01J2219/00286 , B01J2219/00389 , B01J2219/00484 , B01J2219/00488 , B01J2219/0049 , B01J2219/00495 , B01J2219/005 , B01J2219/0059 , B01J2219/00596 , B01J2219/00689 , B01J2219/00695 , B01J2219/00725 , C40B40/10 , C40B60/14
摘要： An apparatus is provided for automatically constructing a polypeptide of high purity, up to 50 amino acids in length, using only single couplings. The apparatus includes an activation system for receiving protected amino acids, one kind at a time, having a common vessel (an activator vessel) in which to activate each of the amino acids. Also included is a reaction vessel for containing a resin used in solid-phase peptide synthesis for attaching a peptide chain thereto. A transfer system is also provided, which operates under control of a computer, to transfer the activated species from the activation system to the reaction vessel and to transfer amino acids, reagents, gases, and solvents from one part of the apparatus to another.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式