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11. 发明申请

US20110045503A1 The Use of Novel Coumarins as Glutathione and Thiol Labels 有权
标题翻译：使用新型香豆素作为谷胱甘肽和硫醇标签
公开(公告)号：US20110045503A1
公开(公告)日：2011-02-24
申请号：US12666256
申请日：2008-07-24
申请人： Kyle Gee , Michael Janes , Iain Johnson , Hee Chol Kang
发明人： Kyle Gee , Michael Janes , Iain Johnson , Hee Chol Kang
IPC分类号： G01N33/52 , C07D471/06
CPC分类号： G01N33/52 , C07D491/06 , C07D491/16 , C07F9/5442 , C07K5/0215 , G01N33/582 , G01N2333/4701 , Y10T436/17 , Y10T436/182
摘要： Fluorescent quinolizinocoumarin compounds substituted with electrophilic reactive groups that bind thiol compounds are described. The compounds are useful in detecting oxidative stress and processes associated therewith in live cells.
摘要翻译：描述了与结合硫醇化合物的亲电子反应性基团取代的荧光喹诺酮香豆素化合物。该化合物可用于检测活细胞中氧化应激和相关过程。

12. 发明申请

US20060160068A1 Competitive immunoassay 有权
标题翻译：竞争性免疫测定
公开(公告)号：US20060160068A1
公开(公告)日：2006-07-20
申请号：US10943463
申请日：2004-09-17
申请人： Joseph Beechem , Kyle Gee , David Hagen , Iain Johnson , Hee-Chol Kang , Christina Pastula
发明人： Joseph Beechem , Kyle Gee , David Hagen , Iain Johnson , Hee-Chol Kang , Christina Pastula
IPC分类号： C12Q1/70 , C12Q1/68 , C07K16/46 , C07F5/02 , C07D311/88
CPC分类号： G01N33/542 , C07F9/12 , C07F9/65318 , C07F9/65522 , C07F9/6596 , C07K16/46 , Y10S435/968 , Y10S436/80 , Y10S530/807 , Y10T436/13
摘要： The present invention provides ligand-detection reagents, ligand analogs and methods for determining the presence of a ligand in a sample. The ligand-detection reagent comprises a ligand-binding antibody and a ligand analog to form an antibody-ligand analog complex wherein the ligand analog is covalently bonded to a reporter molecule. This complex may additionally comprise a labeling protein non-covalently bonded to the antibody to form a ternary complex wherein the labeling protein comprises a monovalent antibody fragment or a non-antibody protein that is covalently bonded to a label moiety. The reporter molecule is either quenched by the ligand-binding antibody or by the label moiety of the labeling protein, depending on the reporter molecule and the ligand-binding antibody, wherein the amount of quenching is directly related to the amount of ligand present in the sample. Alternatively, the ligand analog is fluorogenic wherein the ligand analog is essentially non-fluorescent in solution but when bound by the ligand-binding antibody the detectable signal increases. In this instance a decrease in signal, as opposed to the relieving of quenching, is measured for the presence of a target ligand.
摘要翻译：本发明提供配体检测试剂，配体类似物和用于测定样品中配体存在的方法。配体检测试剂包含配体结合抗体和配体类似物以形成抗体 - 配体类似物复合物，其中配体类似物共价键合到报告分子。该复合物还可以包含与抗体非共价键合以形成三元复合物的标记蛋白质，其中标记蛋白质包含共价键合到标记部分的单价抗体片段或非抗体蛋白质。取决于报道分子和配体结合抗体，报告分子被配体结合抗体或标记蛋白的标记部分淬灭，其中淬灭量与存在于样品。或者，配体类似物是荧光的，其中配体类似物在溶液中基本上是非荧光的，但当被配体结合抗体结合时，可检测信号增加。在这种情况下，与目标配体的存在相比，测量了与消除淬灭相反的信号降低。

13. 发明申请

US20050214807A1 Environmental sensitive fluorogenic compounds and their application for singlet oxygen and protein detection 审中-公开
标题翻译：环境敏感性荧光化合物及其应用于单线态氧和蛋白质检测
公开(公告)号：US20050214807A1
公开(公告)日：2005-09-29
申请号：US10993908
申请日：2004-11-19
申请人： Iain Johnson , Wai-Yee Leung , Jixiang Liu , Brian Patch
发明人： Iain Johnson , Wai-Yee Leung , Jixiang Liu , Brian Patch
IPC分类号： C07D209/54 , C07D491/02 , C09B11/08 , C09B19/00 , C12Q1/68
CPC分类号： C09B19/00 , C09B1/00 , C09B11/08 , C09B11/24 , C09B57/02 , Y10S436/80 , Y10T436/20
摘要： The present invention provides fluorogenic probes and corresponding fluorescent compounds, methods of using the probes, compounds and kits that include the probes.
摘要翻译：本发明提供荧光探针和相应的荧光化合物，使用探针的方法，包括探针的化合物和试剂盒。

14. 发明授权

US08173698B2 Environmental sensitive fluorogenic compounds and their application for singlet oxygen and protein detection 有权
标题翻译：环境敏感性荧光化合物及其应用于单线态氧和蛋白质检测
公开(公告)号：US08173698B2
公开(公告)日：2012-05-08
申请号：US12040850
申请日：2008-02-29
申请人： Iain Johnson , Jixiang Liu , Brian Patch , Wai-Yee Leung
发明人： Iain Johnson , Jixiang Liu , Brian Patch , Wai-Yee Leung
IPC分类号： A61K31/352 , C07D311/82
CPC分类号： C09B19/00 , C09B1/00 , C09B11/08 , C09B11/24 , C09B57/02 , Y10S436/80 , Y10T436/20
摘要： The present invention provides fluorogenic probes and corresponding fluorescent compounds, methods of using the probes, compounds and kits that include the probes.
摘要翻译：本发明提供荧光探针和相应的荧光化合物，使用探针的方法，包括探针的化合物和试剂盒。

15. 发明申请

US20070269902A1 Antibody complexes and methods for immunolabeling 审中-公开
标题翻译：抗体复合物和免疫标记方法
公开(公告)号：US20070269902A1
公开(公告)日：2007-11-22
申请号：US10666291
申请日：2003-09-17
申请人： Joseph Beechem , David Hagen , Iain Johnson
发明人： Joseph Beechem , David Hagen , Iain Johnson
IPC分类号： G01N33/00 , C12Q1/68
CPC分类号： B82Y30/00 , B82Y5/00 , B82Y10/00 , G01N33/53 , G01N33/58 , G01N33/6857
摘要： The present invention provides labeling reagents and methods for labeling primary antibodies and for detecting a target in a sample using an immuno-labeled complex that comprises a target-binding antibody and one or more labeling reagents. The labeling reagents comprise monovalent antibody fragments or non-antibody monomeric proteins whereby the labeling reagents have affinity for a specific region of the target-binding antibody and are covalently attached to a label. Typically, the labeling reagent is an anti-Fc Fab or Fab′ fragment that was generated by immunizing a goat or rabbit with the Fc fragment of an antibody. The present invention provides for discrete subsets of labeling reagent and immuno-labeled complexes that facilitate the simultaneous detection of multiple targets in a sample wherein the immuno-labeled complexes are distinguished by i) a ratio of label to labeling reagent, or ii) a physical property of said label, or iii) a ratio of labeling reagent to said target-binding antibody, or iv) by said target-binding antibody. This is particularly useful for fluorophore labels that can be attached to labeling reagents and subsequently immuno-labeled complexes in ratios for the detection of multiple targets.
摘要翻译：本发明提供标记试剂和用于标记一抗的标记试剂和使用包含靶结合抗体和一种或多种标记试剂的免疫标记复合物检测样品中靶标的方法。标记试剂包含单价抗体片段或非抗体单体蛋白质，由此标记试剂对靶结合抗体的特定区域具有亲和力，并且共价连接到标记物上。通常，标记试剂是通过用抗体的Fc片段免疫山羊或兔而产生的抗Fc Fab或Fab'片段。本发明提供标记试剂和免疫标记的复合物的离散子集，其有助于同时检测样品中的多个靶标，其中通过i）标记物与标记试剂的比率来区分免疫标记的复合物，或ii）物理或iii）标记试剂与所述靶结合抗体的比例，或iv）所述靶结合抗体。这对于可以连接到标记试剂和随后的用于检测多个靶的比例的免疫标记的复合物的荧光团标记物特别有用。

16. 发明申请

US20050208598A1 Biotin recognition sensors and high-throughput assays 审中-公开
标题翻译：生物素识别传感器和高通量测定
公开(公告)号：US20050208598A1
公开(公告)日：2005-09-22
申请号：US11058345
申请日：2005-02-14
申请人： W. Cox , Iain Johnson
发明人： W. Cox , Iain Johnson
IPC分类号： C07D498/02 , C07F5/02 , C12M1/34 , C12Q1/68 , G01N33/53 , G01N33/542 , G01N33/543
CPC分类号： G01N33/5308 , G01N33/542
摘要： In one embodiment is provided a new class of biotin recognition sensors which comprise a biotin recognition compound (containing a biotin binding moiety), a fluorescent donor moiety, and an acceptor moiety. These compounds are useful for detecting biotin in a sample or on a carrier molecule. In addition to compounds, the invention also provides methods and kits for detecting the presence of biotin in a sample or on a carrier molecule.
摘要翻译：在一个实施方案中提供了一类新的生物素识别传感器，其包含生物素识别化合物（含有生物素结合部分），荧光供体部分和受体部分。这些化合物可用于检测样品或载体分子上的生物素。除了化合物之外，本发明还提供用于检测样品或载体分子上生物素的存在的方法和试剂盒。

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